This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hayano, S. Articles by Tanaka, A. Search for Related Content PubMed PubMed Citation Articles by Hayano, S. Articles by Tanaka, A.

 Previous Article  |  Next Article 

J Bacteriol. 1968 May; 95(5): 1551-1554
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Streptococcal Sialidase II. Kinetic and Immunological Studies of Sialidase Produced by Group K Streptococcus

Saitama Institute of Public Health, Omiya, Saitama, Japan

ABSTRACT

Kinetic and immunological studies were carried out on the sialidase produced by strain 6646, group K streptococcus (K-sialidase). The K_m values of K-sialidase were 0.9 mM for sialyllactose and 0.17 mM for bovine submaxillary mucin. The antibody against K-sialidase was produced in rabbits immunized with this enzyme. An assay procedure for determination of the anti-K-sialidase activity in terms of reciprocal of the serum dilution corresponding to the 50% inhibition point is described. Anti-K-sialidase activity is widely distributed in human sera, but this has not yet been found to be correlated with streptococcal diseases, and no definite relationship was proved between the anti-K-sialidase titer and the anti-streptolysin O titer through this study. Anti-K-sialidase serum had no effect on Vibrio cholerae sialidase.