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J Bacteriol. 1968 May; 95(5): 1560-1566
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Rabbit Ileal Loop Response to Strains of Clostridium perfringens¹

^a Department of Foods and Nutrition and the Food Research Institute, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C in Skim Milk (Difco), 14 of 29 type A strains isolated from food-poisoning outbreaks consistently produced exudation of fluid and consequent dilation of the ileal segments. In contrast, 15 of the 18 strains derived from other sources failed to elicit a response. By use of different inoculum preparations, nearly all strains could be made to give at least an occasional positive loop reaction. Diarrhea was not obtained in rabbits by intraluminal injection into the normal ileum or by per os administration of the cultures. Lecithinase, purified and in concentrated culture supernatant fractions, failed to produce a response in the isolated ileal loops.

¹ Published with the permission of the Director of the Wisconsin Agricultural Experiment Station, Madison.

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