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J Bacteriol. 1968 May; 95(5): 1804-1812
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Nature of the Carrier State of Bacteriophage SP-10 in Bacillus subtilis¹

^a Department of Biology, Georgetown University, Washington, D.C. 20007

ABSTRACT

Although the association of phage SP-10 with Bacillus subtilis W-23-S^r persists in heat- and antiserum-resistant form through the spore stage, it is unstable in vegetative cells and frequently terminates in loss of the carried phage or in lysis. On low-tonicity media, the plating efficiency of carrier cells is low. However, high concentrations of succinate or sucrose or a slowed growth rate preserve viability: on 0.48 M succinate-agar, the viable count per optical density unit is the same as that of a noncarrier control culture. Carrier clones retain phage on 0.48 M succinate-agar. At higher succinate levels, many colonies emerge free of phage; at 1 M succinate, all are cured, probably because high succinate inhibits reinfection. Growth of carrier cells in liquid medium with antiphage serum results in rapid curing; events in such cultures with and without succinate were studied quantitatively by tracing the emergence of sensitive cells, the multiplication and induction of carrier cells, and the sensitivity of carrier cells to superinfection with virulent phage. During log phase, 40 to 70% of the carrier cells became sensitive to virulent phage, although the same cells were insensitive during lag and stationary phase. Apparently, fluctuations in repressor levels are responsible. Spontaneous induction of carrier cells followed a qualitatively similar pattern, perhaps in response to changes in level of the same repressor. Production of sensitive segregants by carrier followed a different course, presumably because the repressor does not affect segregation. Many sensitive cells were found two to three divisions after inoculation in antiserum medium. This suggests that each inoculum cell contained one or only a few phage replicons. The data are compatible with the idea that the carrier state in media without antisera is maintained entirely by reinfection and without replication of phage in the latent state. Alternative models which involve replication of latent phage are not ruled out, however.

² Present address: Department of Microbiology, Gunma University School of Medicine, Maebashi, Japan.

¹ A summary of this work was presented at the Annual Meeting of the American Society for Microbiology, Los Angeles, May, 1966.