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J Bacteriol. 1968 June; 95(6): 2035-2043
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Carbohydrate Composition of the Phenol-soluble Lipopolysaccharides of Citrobacter freundii

Rudolf A. Raffa,1 and R. W. Wheatb

a Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27706
b Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27706

ABSTRACT

Phenol-soluble lipopolysaccharides were obtained from the interphase and phenol phase fractions of 44% aqueous phenol-extracted Citrobacter species. Upon detailed investigation of C. freundii 8090, the two lipopolysaccharide fractions were found to contain different amounts of lipid A, although qualitative composition was similar. Both contained lipid A, 2-keto-deoxyoctonic acid, heptose, phosphate, D-glucose, galactose, rhamnose, 2-acetamido-2 deoxy-D-glucose, 3-acetamido-3,6-dideoxy-D-glucose, O-acetyl, and trace amino acids. Partially purified phenol-phase lipopolysaccharide partitioned into the phenol-soluble phase when refractionated with 44% aqueous phenol, and was further found to be soluble in 88% phenol, 95% ethyl alcohol, and chloroform-methanol (2:1).

FOOTNOTES

1 U.S. Public Health Service predoctoral trainee supported by Training Grant 5T01 GM 00233-07. This work was taken in part from a dissertation submitted by R. A. Raff to Duke University in partial fulfillment of the requirements for the Ph.D. degree. Present address: Armed Forces Radiobiology Research Institute, Bethesda, Md. 20014.

J Bacteriol. 1968 June; 95(6): 2035-2043
Copyright © 1968 American Society for Microbiology. All Rights Reserved.





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