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J Bacteriol. 1968 June; 95(6): 2078-2082
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Methylation of the Cellular Lipid of Methionine-requiring Agrobacterium tumefaciens

¹ Pioneering Laboratory for Microbiological Chemistry, Northern Regional Research Laboratory, U.S. Department of Agriculture, Peoria, Illinois 61604

ABSTRACT

Mutants of Agrobacterium tumefaciens requiring methionine for growth on a solid basal medium were induced by the use of N-methyl-N'-nitro-N-nitrosoguanidine. In addition to the difference of mutant strains, the extent of methionine dependency differed in a liquid basal medium and in the presence of aspartate or fumarate. When (¹⁴C-methyl)-methionine was added to strain WM-11 growing in a prescribed basal medium, incorporation of ¹⁴C into the cellular "residue" fraction and polar "N-methylated" lipid fraction depended strictly on cellular growth and on external methionine concentration. However, a net synthesis of the "cyclopropane" fatty acid fraction occurred even during the maximal stationary phase if excess methionine was present.