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J Bacteriol. 1968 June; 95(6): 2242-2248
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Biosynthesis of the O Antigen from Citrobacter 139¹

^a Department of Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461

ABSTRACT

The biosynthesis of the O antigen of Citrobacter 139 (Escherichia coli 3 Zurich 4,5,12:z₂₀) was shown to proceed through a series of lipid-linked intermediates, similar to those involved in O-antigen synthesis in Salmonella. Galactose was the first sugar incorporated, followed by rhamnose and mannose. Abequose was incorporated from cytidine diphosphate (CDP)-abequose only when all three of the other nucleotide sugars (uridine diphosphate galactose, guanosine diphosphate mannose, and thymidine diphosphate rhamnose) were present. Rhamnosyl-galactosyl 1-phosphate and mannosyl-rhamnosyl-galactosyl 1-phosphate were identified as the products of mild alkaline hydrolysis of the lipid-linked intermediates.

² Predoctoral trainee, Public Health Service training grant no. GM 1191. Present address: Harvard University, Biological Laboratories, Cambridge, Mass.

¹ Communication no. 111 from the Joan and Lester Avnet Institute of Molecular Biology.