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J Bacteriol. 1968 July; 96(1): 126-130
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

In Vitro Production of Choleragen and Vascular Permeability Factor by Vibrio cholerae

¹ Department of Microbiology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27103

ABSTRACT

The in vitro production of significant amounts of extracellular choleragen and vascular permeability factor (PF) by Vibrio cholerae strain VC-12 (Ogawa) in a basal peptone medium required forced aeration, low incubation temperature, and a low initial pH. Filtrates of alkaline peptone cultures of VC-12 grown at 37 C contained an ion translocase inhibitory activity but neither choleragen nor PF activity, Sterile filtrates of pH 6.5 peptone cultures of VC-12 grown at 29 C contained no ion translocase inhibitory activity but possessed choleragen activity (lethality for the suckling rabbit) and PF activity to the extent that the intradermal inoculation of 0.1 ml of a 1:12,288 dilution of such a filtrate gave rise to a vascular permeability reaction (8 by 8 mm in diameter) in the guinea pig. PF excretion occurred during the late logarithmic phase of growth but did not appear to be the consequence of cell lysis. The PF activities of strains VC-12 and 569B (Inaba) were neutralized to the same extent by anticholeragen antiserum prepared against crude 569B choleragen.

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