This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hsu, H. S. Articles by Radcliffe, A. S. Search for Related Content PubMed PubMed Citation Articles by Hsu, H. S. Articles by Radcliffe, A. S.

 Previous Article  |  Next Article 

J Bacteriol. 1968 July; 96(1): 191-197
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Interactions Between Macrophages of Guinea Pigs and Salmonellae I. Fate of Salmonella typhimurium Within Macrophages of Normal Guinea Pigs¹

^a Department of Microbiology, Medical College of Virginia, Richmond, Virginia 23219

ABSTRACT

A suspended cell culture procedure was described for the cultivation of guinea pig macrophages infected with Salmonella typhimurium. The fate of the intracellular bacteria was assessed by quantitative recovery of viable bacteria with 0.5% solution of sodium desoxycholate. Two strains of S. typhimurium with different degrees of virulence for mice were compared. There was an initial destruction of intracellular bacteria of both strains; however, the extent of this destruction differed. Approximately 1% of the avirulent bacteria initially phagocytized survived at the end of 4 hr, whereas approximately 8% of the virulent bacteria survived at the end of 3 hr. After this initial killing, the intracellular bacteria began to multiply at a logarithmic rate between 3 and 21 hr after phagocytosis, and then a stationary phase was attained. The rate of this multiplication was comparable for both strains.

² A. D. Williams Graduate Fellow, Medical College of Virginia.

¹ Presented in part at the 4th Annual National Meeting of the Reticuloendothelial Society, Winston-Salem, N.C., December 1967.