This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Khan, P. L. Search for Related Content PubMed PubMed Citation Articles by Khan, P. L.

 Previous Article  |  Next Article 

J Bacteriol. 1968 July; 96(1): 205-214
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Isolation of High-Frequency Recombining Strains from Escherichia coli Containing the V Colicinogenic Factor

¹ College of Biological Sciences, Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101

ABSTRACT

The isolation and characterization of high-frequency recombining strains from different Escherichia coli host cells containing either the F factor or the Col V factor are described. The strains (with one exception) formed from three of the V⁺ parents showed the same origin and polarity of transfer (xyl-arg-pro-trp-his-mal). The Hfr strains formed from the one remaining V⁺ and the F⁺ host cells showed a greater variety in their points of origin. In addition, several Hfr strains isolated from V⁺ parents lost the ability to produce colicin V. F_v⁺ segregants of these were isolated, and the F_v factors appeared to retain their preferential site for Hfr formation, but they lacked other propertes controlled by the Col V factor. Chromosomal integration of episomes and its relation to the fertility of F⁺ and V⁺ strains are discussed. Production of colicin V appeared to be uninfluenced by the state of the Col V factor within the cell.

This article has been cited by other articles:

• Panke, S., Meyer, A., Huber, C. M., Witholt, B., Wubbolts, M. G. (1999). An Alkane-Responsive Expression System for the Production of Fine Chemicals. Appl. Environ. Microbiol. 65: 2324-2332 [Abstract] [Full Text]  
• Stambuk, S., Radman, M. (1998). Mechanism and Control of Interspecies Recombination in Escherichia coli. I. Mismatch Repair, Methylation, Recombination and Replication Functions. Genetics 150: 533-542 [Abstract] [Full Text]  
• Vulic>, M., Dionisio, F., Taddei, F., Radman, M. (1997). Molecular keys to speciation: DNA polymorphism and the control of genetic exchange in enterobacteria. Proc. Natl. Acad. Sci. USA 94: 9763-9767 [Abstract] [Full Text]