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J Bacteriol. 1968 July; 96(1): 39-42
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Isolation of Acinetobacter from Soil and Water

Paul Baumann1

a Department of Bacteriology and Immunology, University of California, Berkeley, California 94720

ABSTRACT

An enrichment culture procedure for isolating members of the genus Acinetobacter from soil and water is described. It involves the use of vigorously aerated mineral media at relative low pH, supplemented with acetate or other suitable carbon source and nitrate as nitrogen source. With this method, virtually all samples of soil and water yielded representatives of this genus. Semiquantitative comparisons of the numbers of Acinetobacter and of all bacteria capable of aerobic growth in a complex medium revealed that Acinetobacter constituted no less than 0.001% of the total heterotrophic aerobic population in soil and water and was one of the predominant organisms in some water samples.


FOOTNOTES

1 Present address: Retina Foundation, Boston, Mass. 02104.


J Bacteriol. 1968 July; 96(1): 39-42
Copyright © 1968 American Society for Microbiology. All Rights Reserved.




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