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J Bacteriol. 1968 July; 96(1): 51-54
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Metabolism of D-Fructose in Aerobacter aerogenes: Analysis of Mutants Lacking D-Fructose 6-Phosphate Kinase and D-Fructose 1,6-Diphosphatase¹

^a Department of Biochemistry, Michigan State University, East Lansing, Michigan 48823

ABSTRACT

The relative significance of the pathways for the conversion of D-fructose to D-fructose 1,6-diphosphate via D-fructose 1-phosphate or D-fructose 6-phosphate in Aerobacter aerogenes PRL-R3 was assessed by observing growth patterns of mutants lacking either D-fructose 6-phosphate kinase or D-fructose 1,6-diphosphatase. The mutant lacking D-fructose 6-phosphate kinase grew well on D-fructose or glycerol but not on D-glucose, whereas the mutant lacking D-fructose 1,6-diphosphatase grew on D-glucose but not on D-fructose or glycerol. The data indicate that the pathway of D-fructose metabolism is primarily through D-fructose 1-phosphate rather than D-fructose 6-phosphate.

² Predoctoral Fellow of the National Defense Education Act.

¹ Michigan Agricultural Experiment Station Journal Article no. 4323.

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