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J Bacteriol. 1968 October; 96(4): 1137-1143
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Cholera Toxins: Purification and Preliminary Characterization of Ileal Loop Reactive Type 2 Toxin

^a Department of Microbiology, University of Chicago, Chicago, Illinois 60637

ABSTRACT

Details for the preparation and partial purification of culture supernatant fluids of Vibrio cholerae (V. comma) 569B which retain rabbit ileal loop fluid-accumulating activity are presented. These preparations were fractionated on Sephadex G-200 and on diethylaminoethyl-Sephadex. On the latter, two fractions were obtained by elution with a linear sodium chloride gradient. The fraction designated "fraction I" retains the toxic activity as demonstrated in the rabbit ileal loop model. Chemical and immunological properties of this active fraction are described.

¹ Research Officer, Indian Council of Medical Research; Research Fellow of the Office of International Research, National Institutes of Health, Public Health Service.

This article has been cited by other articles:

• Finkelstein, R. A., Lospalluto, J. J. (1972). Crystalline Cholera Toxin and Toxoid. Science 175: 529-530 [Abstract]  
• Lewis, A. C., Freeman, B. A. (1969). Separation of Type 2 Toxins of Vibrio cholerae. Science 165: 808-809 [Abstract]