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J Bacteriol. 1968 December; 96(6): 1991-1996
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Fractionated Strands of Bacterial Deoxyribonucleic Acid III. Transformation Efficiencies and Rates of Phenotypic Expression¹

^a Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27706

ABSTRACT

Preferential binding of guanine-rich ribopolymers to one of the complementary strands of denatured deoxyribonucleic acid (DNA) of Diplococcus pneumoniae permitted the fractionation of the complements in CsCl density gradients. Phenotypic expression of the newly acquired genes for four drug resistances was more rapid in cells transformed by the heavy fractions than in those transformed either by light fractions or by unfractionated DNA. The efficiencies of transformation with the two complements were nearly equal for the four markers tested. Both efficiency and expression results were the same whether we assayed the residual activity or the activity obtained by annealing the fractions with excess recipient DNA.

¹ Parts of this study have been abstracted from a Ph.D. dissertation (Duke University, 1968) by J. M. P.