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J Bacteriol. 1968 December; 96(6): 2043-2048
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Production of L-Asparaginase II by Escherichia coli

¹ Department of Microbiology, New York University School of Medicine, New York, New York 10016

ABSTRACT

L-Asparaginase II was synthesized at constant rates by Escherichia coli under anaerobic conditions. The enzyme was produced optimally by bacteria grown between pH 7 and 8 at 37 C. Although some enzyme was formed aerobically, between 100 and 1,000 times more asparaginase II was produced during anaerobic growth in media enriched with high concentrations of a variety of amino acids. Bacteria grown under these conditions should provide a rich starting material for the large-scale production of the enzyme. No single amino acid specifically induced the synthesis of the asparaginase, nor did L-asparagine, even when it was used as the only source of nitrogen. The enzyme was produced at lower rates in the presence of sugars; glucose was the most inhibitory.

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