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J Bacteriol. 1969 February; 97(2): 848-860
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Chromosome Replication in Salmonella typhimurium¹

^a Division of Biology, Kansas State University, Manhattan, Kansas 60502

ABSTRACT

The replication of the Salmonella typhimurium chromosome was studied. As with E. coli 15T^–, replication was sequential. After amino acid starvation, replication proceeded from a unique and heritable region of the chromosome. 5-Bromouracil, when substituted for thymine, did not disturb the sequence of replication nor did it initiate extra replication cycles. By labeling the origin and the terminus of the chromosome with ³H- and ¹⁴C-thymine, respectively, it was possible to determine that the rate of chain elongation decreases as the growth rate decreases. No gap in the replication cycle could be observed.

¹ This research has been submitted by Hilary Chan as part of a thesis in fulfillment of the requirements for the Ph.D. degree.