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J Bacteriol. 1969 February; 97(2): 867-872
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Gene-Enzyme Relationships in Neurospora Invertase¹

^a Department of Biological Sciences, Stanford University, Stanford, California 94305

ABSTRACT

A spontaneous, single-gene mutation responsible for a total lack of invertase activity in Neurospora crassa is described. The mutation is believed to lie in the structural gene for invertase, since an immunologically cross-reacting protein is made by the mutant strain. In addition, there was no evidence for a defect in regulation of invertase activity or synthesis by the following criteria. (i) The invertaseless condition was recessive in heterokaryons; (ii) no invertase inhibitor was found in mutant extracts by mixing experiments; and (iii) none of the several sugars able to induce activity in wild-type strains was able to induce activity in the mutant strain. It was also discovered that most of the wild-type enzyme (55 to 75%) cannot be washed free from the rapidly sedimenting cell debris. This finding provided additional support for the hypothesis that Neurospora invertase is located within or about the cell wall.

² Present address: Department of Botany, University of Illinois, Urbana, Ill. 61801.

¹ Taken in part from a thesis submitted by M. L. S. to Stanford University in partial fulfillment of the requirements for the Ph.D. degree.