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J Bacteriol. 1969 March; 97(3): 1009-1011
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Preparation of Cell Walls and Protoplasm of Neisseria with the Ribi Cell Fractionator

^a Venereal Disease Research Laboratory, Venereal Disease Program, National Communicable Disease Center, Atlanta, Georgia 30333

ABSTRACT

The varied pressures required for disruption of Neisseria gonorrhoeae and other species of Neisseria when the Sorvall-Ribi refrigerated cell fractionator is used in the preparation of cell walls and cellular protoplasm are reported. Optimal disruption pressure for the gonococcus was considerably less than that required for other members of the genus Neisseria. Pressures varied from 8,000 psi for N. gonorrhoeae F62, colony type 4, to 22,000 psi for the nonpathogenic Neisseria—N. sicca, N. flava, and N. catarrhalis. Representative electron photomicrographs are shown.

¹ Permanent address: National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratory, Hamilton, Mont. 59840.

² Deceased 18 July 1968.