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J Bacteriol. 1969 April; 98(1): 1-3
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Plaquing of Toxoplasma gondii in Secondary Cultures of Chick Embryo Fibroblasts

¹ Palo Alto Medical Research Foundation, Division of Allergy, Immunology, and Infectious Diseases, and Stanford University School of Medicine, Division of Infectious Diseases, Palo Alto, California 94301

ABSTRACT

A tissue culture system that employed chick embryo fibroblasts was described for plaquing of the obligate intracellular parasite Toxoplasma gondii. High plaquing efficiency and reproducibility were accomplished by the use of secondary rather than primary cultures and the use of toxoplasma obtained from disrupted peritoneal cells of mice infected 48 hr earlier. The monolayers were cultured in a special medium which maintained the fibroblasts until the maximal number of plaques was produced. Optimal plaque formation was obtained in 5 days, and the plaques were easily counted macroscopically.