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J Bacteriol. 1969 April; 98(1): 16-22
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Lipids of Pseudomonas aeruginosa Cells Grown on Hydrocarbons and on Trypticase Soy Broth¹

^a Department of Biology, University of Dayton, Dayton, Ohio 45409

ABSTRACT

Lipids were extracted from cells of Pseudomonas aeruginosa grown on a pure hydrocarbon (tridecane), mixed hydrocarbons (JP-4 jet fuel), and on Trypticase Soy Broth. Total lipids produced from each substrate represented from 7.1 to 8.2% of cellular dry weight, of which 5.0 to 6.4% were obtained before cellular hydrolysis (free lipids) and 1.7 to 2.0% were extracted after cellular hydrolysis (bound lipids). Free lipids from cells grown on each medium were separated into four fractions by thin-layer chromatography. All fractions were present in cells from each type of medium, and the "neutral fraction" constituted the largest fraction. The fatty acid composition of free lipids was determined by gas-liquid chromatography. Cells grown on each medium contained saturated and unsaturated C₁₄ to C₂₀ fatty acids. Trace amounts of C₁₃ fatty acids were found in tridecane-grown cells. Saturated C₁₆ and C₁₈ were the major acids present in all cells. Quantitative differences were found in fatty acids produced on the three media, but specific correlations between substrate carbon sources and fatty acid content of cells were not evident. Tridecane-grown cells contained only traces of C₁₃ acid and small amounts of C₁₅ and C₁₇ acids, suggesting that the organism's fatty acids were derived from de novo synthesis rather than by direct incorporation of the hydrocarbon.

¹ This paper represents part of a thesis submitted by the senior author to the Graduate School, University of Dayton, in partial fulfillment of the requirements for the M.S. degree.