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J Bacteriol. 1969 July; 99(1): 18-24
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Ammonium Sulfate Coprecipitation Antibody Determination with Purified Staphylococcal Enterotoxins

¹ Medical Sciences Laboratory, Fort Detrick, Frederick, Maryland 21701

ABSTRACT

The ammonium sulfate coprecipitation technique of Farr was applied in a study of the purified enterotoxins of Staphylococcus aureus. Ammonium sulfate coprecipitation of iodine-131-labeled enterotoxins A, B, and C, with the use of a 1.6 M concentration of (NH₄)₂SO₄, revealed differences in the antigen-binding capacity of normal and immune rabbit sera for the enterotoxins. The coprecipitation technique provided a quantitative test for detecting antibody to enterotoxin that was more sensitive than agar-gel diffusion methods. Antigen-binding tests suggested the presence of similar antigenic determinant groups in all three toxins. Measurable antigenbinding capacities for enterotoxins A, B, and C were detected in sera of normal human subjects and became elevated in several subjects accidently exposed to enterotoxin.