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J Bacteriol. 1969 July; 99(1): 238-241
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Escherichia coli K-12 Mutants Resistant to Nalidixic Acid: Genetic Mapping and Dominance Studies1

Michael W. Hane2 and Thomas H. Wood

a Department of Physics, University of Pennsylvania, Philadelphia, Pennsylvania 19104

ABSTRACT

Escherichia coli K-12 strains tested so far (approximately 20) can be separated into three groups on the basis of their abilities to form colonies on nutrient agar supplemented with nalidixic acid (NAL): (i) Nals or wild type (no growth at 1 to 2 µg/ml); (ii) NalAr (growth at 40 µg/ml or higher); and (iii) NalBr (growth at 4 µg/ml, but no growth at 10 µg/ml). The NalAr group has a spectrum of sensitivity ranging from 60 to over 100 µg/ml. All Hfr strains of the NalAr and NalBr groups transfer NAL resistance to recipient cells at genetic loci which are at 42.5 ± 0.5 and 51 ± 1 min, respectively, on the Taylor-Trotter map. Some members of the NalAr group also have the genetic locus for NalBr. The nalAs allele is completely dominant to nalAr in a partial diploid configuration. In haploids, nalAr-nalBr is phenotypically NalAr; nalAr-nalBs is NalAr; and nalAs-nalBr is NalBr. The map location of nalA and the easy differentiation between NalAr and NalAs allow this marker to be used as a counterselector in bacterial conjugation experiments.

FOOTNOTES

2 Present address: Department of Molecular Biology, University of California, Berkeley, Calif.

1 The data presented in this paper are taken from a thesis submitted to the Committee on Molecular Biology, University of Pennsylvania, by M. W. Hane in partial fulfillment of the requirements for the Ph. D. degree.

J Bacteriol. 1969 July; 99(1): 238-241
Copyright © 1969 American Society for Microbiology. All Rights Reserved.



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