This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Moore, R. L. Articles by McCarthy, B. J. Search for Related Content PubMed PubMed Citation Articles by Moore, R. L. Articles by McCarthy, B. J.

 Previous Article  |  Next Article 

J Bacteriol. 1969 July; 99(1): 248-254
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Characterization of the Deoxyribonucleic Acid of Various Strains of Halophilic Bacteria

^a Departments of Microbiology and Genetics, University of Washington, Seattle, Washington 98105

ABSTRACT

Bacteria classified as extreme halophiles, in the genera Halobacterium and Halococcus, contain deoxyribonucleic acid (DNA) which displays two components in a CsCl equilibrium density gradient. The base composition of the major DNA component ranges from 66 to 68% guanine plus cytosine (GC), whereas that of the satellite DNA comprising some 11 to 36% of the total, is between 57 and 60% GC. Purification of the bacterial cells in a CsCl density gradient and other more conventional strain purification procedures both indicated that the presence of the satellite DNA component is not a result of mixed cultures.

¹ Present address: Department of Microbiology and Public Health, Michigan State University, East Lansing, Mich. 48823.

This article has been cited by other articles:

• Kim, J.-m., DasSarma, S. (1996). Isolation and Chromosomal Distribution of Natural Z-DNA-forming Sequences in Halobacterium halobium. J. Biol. Chem. 271: 19724-19731 [Abstract] [Full Text]  
• Kim, J.-m., Yang, C.-f., DasSarma, S. (1996). Analysis of Left-handed Z-DNA Formation in Short d(CG)[IMAGE] Sequences in Escherichia coli and Halobacterium halobium Plasmids. J. Biol. Chem. 271: 9340-9346 [Abstract] [Full Text]  
• Rosenshine, I, Tchelet, R, Mevarech, M (1989). The mechanism of DNA transfer in the mating system of an archaebacterium. Science 245: 1387-1389 [Abstract]  
• Fox, G., Stackebrandt, E, Hespell, R., Gibson, J, Maniloff, J, Dyer, T., Wolfe, R., Balch, W., Tanner, R., Magrum, L., Zablen, L., Blakemore, R, Gupta, R, Bonen, L, Lewis, B., Stahl, D., Luehrsen, K., Chen, K., Woese, C. (1980). The phylogeny of prokaryotes. Science 209: 457-463  
• Ng, W. V., Kennedy, S. P., Mahairas, G. G., Berquist, B., Pan, M., Shukla, H. D., Lasky, S. R., Baliga, N. S., Thorsson, V., Sbrogna, J., Swartzell, S., Weir, D., Hall, J., Dahl, T. A., Welti, R., Goo, Y. A., Leithauser, B., Keller, K., Cruz, R., Danson, M. J., Hough, D. W., Maddocks, D. G., Jablonski, P. E., Krebs, M. P., Angevine, C. M., Dale, H., Isenbarger, T. A., Peck, R. F., Pohlschroder, M., Spudich, J. L., Jung, K.-H., Alam, M., Freitas, T., Hou, S., Daniels, C. J., Dennis, P. P., Omer, A. D., Ebhardt, H., Lowe, T. M., Liang, P., Riley, M., Hood, L., DasSarma, S. (2000). From the Cover: Genome sequence of Halobacterium species NRC-1. Proc. Natl. Acad. Sci. USA 97: 12176-12181 [Abstract] [Full Text]