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J Bacteriol. 1969 July; 99(1): 269-273
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Expression of the Arginine Regulon of Escherichia coli W: Evidence for a Second Regulatory Gene1

Elizabeth C. Theil, George W. Forsyth and Evan E. Jones

a Nutritional Biochemistry Section, Department of Animal Science, and the Department of Biochemistry, North Carolina State University, Raleigh, North Carolina 27607

ABSTRACT

Effect of the M (modifier) gene of Escherichia coli W on the expression of wild-type structural genes of four arginine biosynthetic enzymes was studied by examining enzyme activity in cell-free extracts of cultures grown in minimal medium and medium containing arginine. The mutant M gene was originally identified as causing arginine-induced synthesis of acetylornithine {delta}-transaminase in a strain deficient for the enzyme. The strains used in this study received the mutant M gene by recombination. Noncoordinate repression has been demonstrated for two more enzymes of the arginine regulon of E. coli W and the M– gene increases the degree of noncoordinate repression for the regulon. Mutation of the M gene results in altered regulation of acetylornithine {delta}-transaminase, ornithine transcarbamylase, and acetylornithinase. In addition, a decreased growth rate is observed. It is proposed that the M gene is a regulatory gene. A model is presented to explain the data which involves changes in operator-repressor affinity for the structural genes and possibly for the gene controlling arginyl transfer ribonucleic acid synthetase.

FOOTNOTES

1 Paper no. 2821 of the Journal Series of the North Carolina State University Agricultural Experiment Station, Raleigh.

J Bacteriol. 1969 July; 99(1): 269-273
Copyright © 1969 American Society for Microbiology. All Rights Reserved.





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