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J Bacteriol. 1969 July; 99(1): 274-278
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Transduction of Merodiploidy: Induced Duplication of Recipient Genes

^a Department of Biochemistry, Stanford University School of Medicine, Palo Alto, California 94305

ABSTRACT

Escherichia coli PB160, which carries a tandem duplication with the gene order metB⁺argH^–su₁₅₉⁺thi⁺: metB⁺argH⁺su₁₅₉^–thi⁺, was used to study the mechanism of P1 transduction of genes in the duplicated region. Transduction of the su₁₅₉⁺ allele contained within the duplicated segment yields two kinds of su₁₅₉⁺ recombinants: 91% are haploid su₁₅₉⁺ and 9% are su₁₅₉⁺/su₁₅₉^– merodiploids. The duplication in these merodiploid transductants includes the metB locus; however, both copies of the metB locus usually are derived from the recipient. Thus, the requirements for transduction of the "condition of merodiploidy" appear to be the cotransduction of the repeat point (the region where the duplication begins to repeat itself) and, of course, the selected marker (in this case su₁₅₉⁺). A mechanism whereby two recipient chromosomes interact with the transduced "repeat point" region to regenerate the tandem duplication is implicated. It appears that a duplication much larger than the quantity of genetic material carried by a P1 phage can be produced in a transductant.

¹ Present address: Department of Biological Chemistry, Pennsylvania State University, Milton S. Hershey Medical Center, Hershey, Pa. 17033.

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