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J Bacteriol. 1969 July; 99(1): 51-56
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Occult Endotoxin in Bacterial Protoplasm

National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratory, Hamilton, Montana 59840

ABSTRACT

Protoplasm separated from disrupted cells of gram-negative bacteria was extracted with hot phenol-water or was precipitated with ethyl alcohol after digestion with Pronase. These methods recovered about 10 times more endotoxin than was detectable in the untreated protoplasm. Inactivation of endotoxin by protoplasm also occurred in vitro when the endotoxin was first dissociated into subunits before reaction with protoplasm. Despite this increased yield from another source, the major proportion of endotoxin was still found in the cell walls.