This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lehrer, R. I. Search for Related Content PubMed PubMed Citation Articles by Lehrer, R. I.

Next Article 

J Bacteriol. 1969 August; 99(2): 361-365
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Antifungal Effects of Peroxidase Systems

¹ Cancer Research Institute and Department of Medicine, University of California Medical Center, San Francisco, California 94122

ABSTRACT

In the presence of hydrogen peroxide and either potassium iodide, sodium chloride, or potassium bromide, purified human myeloperoxidase was rapidly lethal to several species of Candida. Its candidacidal activity was inhibited by cyanide, fluoride, and azide, and by heat inactivation of the enzyme. A hydrogen peroxidegenerating system consisting of D-amino acid oxidase, flavine-adenine dinucleotide, and D-alanine could replace hydrogen peroxide in the candidacidal system. Horseradish peroxidase and human eosinophil granules also exerted candidacidal activity in the presence of iodide and hydrogen peroxide; however, unlike myeloperoxidase or neutrophil granules, these peroxidase sources were inactive when chloride replaced iodide. Cells of Saccharomyces, Geotrichum, and Rhodotorula species, and spores of Aspergillus fumigatus and A. niger were also killed by the combination of myeloperoxidase, iodide, and hydrogen peroxide. Peroxidases, functionally linked to hydrogen peroxide-generating systems, could provide phagocytic cells with the ability to kill many fungal species.

This article has been cited by other articles:

• Bellocchio, S., Gaziano, R., Bozza, S., Rossi, G., Montagnoli, C., Perruccio, K., Calvitti, M., Pitzurra, L., Romani, L. (2005). Liposomal amphotericin B activates antifungal resistance with reduced toxicity by diverting Toll-like receptor signalling from TLR-2 to TLR-4. J Antimicrob Chemother 55: 214-222 [Abstract] [Full Text]  
• Reeves, E. P., Nagl, M., Godovac-Zimmermann, J., Segal, A. W. (2003). Reassessment of the microbicidal activity of reactive oxygen species and hypochlorous acid with reference to the phagocytic vacuole of the neutrophil granulocyte. J Med Microbiol 52: 643-651 [Abstract] [Full Text]  
• Marodi, L., Tournay, C., Kaposzta, R., Johnston, R. B. Jr., Moguilevsky, N. (1998). Augmentation of Human Macrophage Candidacidal Capacity by Recombinant Human Myeloperoxidase and GranulocyteMacrophage Colony-Stimulating Factor. Infect. Immun. 66: 2750-2754 [Abstract] [Full Text]  
• Weiss, S., Test, S., Eckmann, C., Roos, D, Regiani, S (1986). Brominating oxidants generated by human eosinophils. Science 234: 200-203 [Abstract]  
• Klebanoff, S. J. (1970). Myeloperoxidase: Contribution to the Microbicidal Activity of Intact Leukocytes. Science 169: 1095-1097 [Abstract]