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J Bacteriol. 1969 August; 99(2): 450-458
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Biosynthesis of Ubiquinone in Escherichia coli K-12: Location of Genes Affecting the Metabolism of 3-Octaprenyl-4-hydroxybenzoic Acid and 2-Octaprenylphenol

Department of Biochemistry, John Curtin School of Medical Research, Australian National University, Canberra, A.C.T., Australia

ABSTRACT

Two genes (ubiB and ubiD) concerned with two successive reactions in ubiquinone biosynthesis in Escherichia coli were mapped and found to be closely linked. Mutant strains of E. coli carrying the ubiB^– and ubiD^– alleles were shown to accumulate 2-octaprenylphenol and 3-octaprenyl-4-hydroxybenzoic acid, respectively. These compounds were isolated and identified by using nuclear magnetic resonance and mass and infrared spectroscopy. Cell extracts from the mutant strain carrying the ubiD^– allele lack 3-octaprenyl-4-hydroxybenzoate decarboxylase activity.

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