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J Bacteriol. 1969 August; 99(2): 480-486
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Biosynthesis of Glucosyl Diglycerides by Mycoplasma laidlawii Strain B

Department of Microbiology, School of Medicine, University of South Dakota, Vermillion, South Dakota 57069

ABSTRACT

Monoglucosyl diglyceride is synthesized from 1,2-diglyceride and uridine-5'-diphosphoglucose (UDP); diglucosyl diglyceride from monoglucosyl diglyceride, and uridine-5'-diphosphoglucose by membranes of Mycoplasma laidlawii strain B. All of these enzymatic activities reside in the membrane. Membranes solubilized by detergent action or succinylation and acetone powders of membranes were inactive. Requirements for Mg²⁺, UDP, and appropriate lipid acceptor were demonstrated for biosynthesis of both glycolipids. Glucose-1-phosphate plus uridine triphosphate could replace the UDP requirement. A medium of relatively high ionic strength and a critical concentration of sodium lauryl sulfate stimulated biosynthesis of the monoglucosyl diglyceride. The optimal pH for both reactions was 8.0. A specificity for 1,2-diglyceride from the homologous organism was found for optimal synthesis of the monoglucosyl diglyceride, and a specificity for monoglucosyl diglyceride was found in the case of diglucosyl diglyceride synthesis. Both reactions were specific for UDP.

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• Li, L., Karlsson, O. P., Wieslander, A. (1997). Activating Amphiphiles Cause a Conformational Change of the 1,2-Diacylglycerol 3-Glucosyltransferase from Acholeplasma laidlawii Membranes According to Proteolytic Digestion. J. Biol. Chem. 272: 29602-29606 [Abstract] [Full Text]  
• Karlsson, O. P., Dahlqvist, A., Vikstrom, S., Wieslander, A. (1997). Lipid Dependence and Basic Kinetics of the Purified 1,2-Diacylglycerol 3-Glucosyltransferase from Membranes of Acholeplasma laidlawii. J. Biol. Chem. 272: 929-936 [Abstract] [Full Text]