INTRODUCTION

Top Abstract Introduction Materials & Methods Results Discussion References

Several bacteria, including some strains of Mycobacterium, Xanthobacter, and Nocardia species, are capable of growth with aliphatic alkenes as carbon and energy sources (8, 9, 20). The pathway for alkene metabolism in these organisms involves an initial monooxygenase-catalyzed reaction producing epoxide intermediates, as illustrated for the substrate propylene and the product epoxypropane in equation 1:

(1)

While the monooxygenase-catalyzed epoxidation of aliphatic alkenes is a well-characterized reaction (13, 17), less is known about the subsequent metabolism of the epoxide intermediates thus formed. The only characterized pathway for an aliphatic-alkene-utilizing bacterium is that of Xanthobacter strain Py2. In this bacterium, epoxides are further metabolized via a novel ring-opening and carboxylation reaction that requires CO₂ as a cosubstrate and forms -keto acids as products (1, 18). Epoxide carboxylase, the enzyme catalyzing this reaction, requires an oxidant (NAD⁺) and a reductant (NADPH or a dithiol) (21), which are reduced and oxidized in the course of epoxide carboxylation as illustrated in equation 2 (3):

(2)

The epoxide carboxylase complex from Xanthobacter strain Py2 has recently been purified and found to consist of four separate proteins (3): component I, a homohexameric protein (41.7-kDa subunits) that is believed to contain the epoxide binding and/or activation site(s); component II, a dimeric flavoprotein (57-kDa subunits) identified as an NADPH:disulfide oxidoreductase which is proposed to generate a reduced thiol(s) essential for catalysis (19); component III, a dimeric protein (26- and 26.2-kDa subunits) with an unidentified catalytic role; and component IV, a homodimeric protein (25.4-kDa subunits) which also has an unidentified catalytic role (3). The mechanistic details involving the interplay of the above-mentioned protein components and cofactors of this complex enzyme have not been elucidated. A preliminary mechanism involving a reductive epoxide ring-opening step, which is followed by an NAD⁺-dependent oxidation step prior to product release, has been proposed (21). Interestingly, in the absence of CO₂, epoxide carboxylase catalyzes the isomerization of aliphatic epoxides to form ketones, although this reaction is apparently of no physiological significance (15, 16). This isomerase activity is, however, relevant to the catalytic mechanism since it demonstrates that a reaction intermediate can alternatively undergo carboxylation to form a -keto acid or protonation to form a ketone.

Epoxide carboxylation represents a new and novel strategy for biological epoxide activation and transformations. To date, it is the only strategy that has been identified for aliphatic epoxide metabolism by the class of bacteria that grow with short-chain aliphatic alkenes as carbon and energy sources. The question of whether epoxide carboxylation is the predominant strategy used to metabolize epoxides in aliphatic-alkene-utilizing bacteria or whether other characterized transformations (i.e., isomerization [10, 14] or hydration [5, 11]) are utilized as well has been raised. One organism that may aid in addressing this question is the gram-positive bacterium Nocardia corallina B276. This bacterium, isolated with propylene as the source of carbon and energy (8), has been reported to convert 1-alkenes ranging from C₂ to C₁₈ in chain length to 1-epoxides, as well as to convert styrene to styrene oxide (7). The alkene monooxygenase which catalyzes these reactions has recently been isolated and shown to be a multiprotein enzyme system (13). However, the pathway(s) by which epoxides are further metabolized in N. corallina B276 has not been studied.

In the present paper, evidence that epoxides are metabolized in a CO₂-dependent manner in N. corallina, similar to that described for Xanthobacter strain Py2, is presented. The reaction requirements and enzymes involved in epoxide carboxylation in N. corallina B276 are described and compared to those of the epoxide carboxylase reaction of Xanthobacter strain Py2. The similarities and differences between these two epoxide carboxylation systems are of interest in the ongoing effort to gain insight into microbial strategies for the metabolism of epoxides.

	MATERIALS AND METHODS

Top Abstract Introduction Materials & Methods Results Discussion References

Chemicals. Tris(hydroxymethyl)aminomethane (Tris) buffer was purchased from Sigma Chemical Co. Horseradish peroxidase color development reagent and a goat anti-rabbit immunoglobulin G-horseradish peroxidase conjugate were purchased from Bio-Rad. Epoxypropane (99% minimum) and 1,2-epoxybutane (99% minimum) were purchased from Aldrich Chemical Co. Methylepoxypropane was purchased from Lancaster Laboratories.

Growth of bacteria and preparation of cell suspensions. Cultures of N. corallina B276 (ATCC 31338) were grown in sealed 4-liter shake flasks containing 0.8 liter of mineral salts medium (1) with propylene (10% [vol/vol] gas phase) or glucose (10 g/liter) as the carbon source. Cultures were harvested at an A₆₀₀ of between 1.0 and 3.0 by centrifugation and washed in buffer (50 mM potassium phosphate, pH 7.2) as described previously (1). Cultures of Xanthobacter strain Py2 were grown on propylene (10% [vol/vol] gas phase) or glucose (10 g/liter) as the carbon source and harvested as previously described (1). Cell pastes were stored at 80°C.

Assay of epoxide degradation activity in whole-cell suspensions. Assays of epoxyalkane degradation by whole-cell suspensions of N. corallina were performed with shaking at 30°C in sealed 9-ml serum vials containing cells, substrate (2 µmol), and buffer (50 mM potassium phosphate, pH 7.2) in a total volume of 1 ml (18). For whole-cell assays performed in the absence of CO₂, residual CO₂ was removed by sparging buffers and flushing sealed vials with CO₂-free nitrogen and including a KOH-saturated filter trap in each of the vials.

Preparation of cell extracts and purified epoxide carboxylase components. Frozen cell paste (15 to 30 g) of N. corallina or Xanthobacter strain Py2 was thawed and resuspended in 2 volumes of buffer (50 mM Tris-HCl [pH 8.2] containing 1 mM dithiothreitol [DTT], 10% [vol/vol] glycerol, DNase I [0.2 mg/ml], and lysozyme [0.3 mg/ml]). The cell suspension was passed four times through a French pressure cell at 110,000 kDa, and the lysate was clarified by centrifugation at 137,000 × g for 30 min at 4°C. After removal of cell debris, the supernatant was dialyzed for 16 h at 4°C against buffer (50 mM Tris-HCl [pH 8.2] containing 10% [vol/vol] glycerol). The dialysate was stored at 80°C and used as the source of enzyme. Methylepoxypropane-treated cell extracts of N. corallina and Xanthobacter strain Py2 were prepared by a method described previously (2). Purified epoxide carboxylase components I to IV from Xanthobacter strain Py2 were prepared as previously described (2, 3).

Assay of epoxide carboxylase activity in cell extracts. Epoxide carboxylase activity was measured by monitoring the time-dependent depletion of epoxypropane by gas chromatography as described previously (1). Assays were performed in sealed 9-ml serum vials containing a source of enzyme (cell extract alone or cell extract plus purified epoxide carboxylase components), in 50 mM Tris-HCl (pH 8.2) containing 10% glycerol, using reagents and reaction conditions described previously (1, 2). For assays performed in the absence of CO₂, CO₂ was removed as described above. Acetoacetate, acetone, and 1,2-epoxybutane were quantified by gas chromatography as previously described (1). Products of epoxyalkane carboxylation were also identified by using ¹³C-enriched NaHCO₃ in the assay mix and analyzing the reaction products by proton-decoupled ¹³C nuclear magnetic resonance spectrometry (NMR) as described previously (1).

Induction of epoxypropane-degrading activity in batch cultures. N. corallina cells which had been grown for several generations with either glucose or propylene as the carbon source were used for induction experiments as previously described (6).

SDS-PAGE and immunoblotting procedures. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (12% total gel; 2.7% cross-linker running gel) was performed in a Mini-Protean II apparatus (Bio-Rad) by the Laemmli procedure (12). Electrophoresed proteins were visualized by Coomassie blue staining. Apparent molecular masses of polypeptides were determined by comparison with the R_f values for molecular mass standard proteins. The standards were bovine serum albumin (66.2 kDa), ovalbumin (45 kDa), carbonic anhydrase (31 kDa), and cytochrome c (12.3 kDa). Immunoblot analysis was conducted as described previously, using a polyclonal antiserum raised against Xanthobacter strain Py2 epoxide carboxylase component I or epoxide carboxylase component II (2).

Protein determination. Protein concentrations were determined by a modified biuret assay with bovine serum albumin as the standard (4). The protein concentration of epoxide carboxylase component II from Xanthobacter strain Py2 was routinely determined by using its reported extinction coefficient (2).

	RESULTS

Top Abstract Introduction Materials & Methods Results Discussion References

CO₂-dependent epoxide degradation in whole-cell suspensions of N. corallina B276. While the oxidation of aliphatic alkenes to epoxides is a well-characterized reaction in N. corallina (8, 13), the pathway by which epoxides are further converted to central metabolites has not been investigated. As an initial step in characterizing the epoxide-converting reaction, the degradation of two aliphatic epoxides, epoxypropane and 1,2-epoxybutane, was investigated using whole-cell suspensions of propylene-grown N. corallina. As shown in Fig. 1, both epoxides were degraded in the presence of CO₂ and bicarbonate but not in their absence. These results suggest a role for CO₂ as a cosubstrate in epoxide degradation similar to that identified in Xanthobacter strain Py2; i.e., aliphatic epoxides are carboxylated to form the corresponding -keto acids. Interestingly, in the absence of CO₂, cell suspensions and cell extracts of propylene-grown Xanthobacter cells catalyze the isomerization of aliphatic epoxides to form the corresponding ketones (acetone in the case of epoxypropane isomerization; methyl ethyl ketone in the case of 1,2-epoxybutane isomerization) at rates approximately 40% lower than those observed for epoxide carboxylation (1, 18). This epoxide isomerization has been proposed to be a fortuitous reaction of no physiological importance (16). To determine whether a similar CO₂-dependent isomerization of epoxides occurs in N. corallina, the rates of epoxypropane and 1,2-epoxybutane degradation were measured in the absence of CO₂. As shown in Fig. 1, no significant degradation of either substrate was observed during the time frame of these assays. These results suggest either that the epoxide-converting enzyme of N. corallina does not catalyze epoxide to ketone isomerization or that the rate of isomerization, relative to that of carboxylation, is much lower than that in the corresponding Xanthobacter enzyme system.

View larger version (20K): [in this window] [in a new window]   FIG. 1.   Requirement of CO2 for epoxyalkane degradation by propylene-grown N. corallina. Assays were performed with whole-cell suspensions (0.5 mg of protein). Closed symbols, assays performed with CO2 and NaHCO3 (50 mM combined concentration); open symbols, assays performed without CO2 and NaHCO3; squares, epoxypropane remaining; circles, 1,2-epoxybutane remaining.

Carboxylation of epoxides to -keto acids in cell extracts of N. corallina. As mentioned in the introduction and shown in equation 2, epoxide carboxylase from Xanthobacter strain Py2 couples the carboxylation of epoxyalkanes to the transhydrogenation of NAD⁺ and NADPH. While NADPH is believed to be the physiological reductant for the reaction, dithiols (e.g., DTT) are capable of substituting for NADPH. With these requirements in mind, the in vitro degradation of epoxypropane and epoxybutane in cell extracts of N. corallina was investigated. As observed for extracts of Xanthobacter strain Py2 (1, 21), a time-dependent consumption of epoxypropane or 1,2-epoxybutane was observed in assay mixtures that contained CO₂, NAD⁺, and either NADPH or DTT. In the absence of both NADPH (or DTT) and NAD⁺, or in the individual absence of either reductant or oxidant, the rates of epoxyalkane degradation were approximately five- to sevenfold lower at the outset of the assays and decreased during the course of the assays. These results suggest that epoxide degradation in N. corallina is coupled to the transhydrogenation of NAD⁺ and NADPH, as has been previously shown to occur for the epoxide carboxylase system of Xanthobacter strain Py2 (3). To identify the products of epoxyalkane degradation, assays were performed with cell extract in the presence of NaH¹³CO₃ and the reaction products were analyzed by ¹³C NMR. The ¹³C NMR spectrum of the reaction products formed on complete consumption of epoxypropane and 1,2-epoxybutane showed resonance peaks with chemical shifts identical to the C-1 (carboxyl) carbon atoms of acetoacetate and 3-keto-pentanoic acid, respectively (data not shown).

View larger version (20K): [in this window] [in a new window]   FIG. 2.   Effect of CO2 on the time courses of epoxypropane degradation and acetoacetate formation catalyzed by cell extracts of N. corallina. Assays were performed with 7.5 mg of cell extract. Closed symbols, assays performed with CO2 and NaHCO3 (60 mM); open symbols, assays performed without CO2 and NaHCO3; squares, epoxypropane remaining; circles, acetoacetate formed; triangles, acetone formed.

Induction of epoxide-degrading activity in N. corallina and identification of inducible polypeptides. The alkene monooxygenase and epoxide carboxylase enzymes in Xanthobacter strain Py2 are inducible proteins that are not expressed in cultures grown on other carbon sources, such as glucose or acetone (6, 16, 18). Induction of alkene monooxygenase and epoxide carboxylase activities by the addition of alkenes or epoxides to glucose-grown Xanthobacter strain Py2 results in the de novo synthesis of new polypeptides, several of which are expressed at high levels and are hence readily visible on SDS-PAGE gels of cell extracts (6). Several of these polypeptides represent components of the alkene monooxygenase and epoxide carboxylase enzymes. For example, the polypeptides constituting epoxide carboxylase components I (42 kDa) and II (57 kDa) and two subunits of the three-subunit alkene monooxygenase (the 43- and 53-kDa subunits) are readily visible in gels of cell extracts (2, 6, 17). To obtain more information about similarities and possible differences between the Xanthobacter and N. corallina epoxide carboxylases, we investigated whether the alkene- and epoxide-degrading enzymes of N. corallina were similarly induced and, if so, whether similar patterns of polypeptides could be observed in induced cells.

View larger version (23K): [in this window] [in a new window]   FIG. 3.   Requirement of new protein synthesis for the degradation of propylene and epoxypropane by N. corallina grown with glucose as the carbon source. Assays were conducted in 150-ml serum bottles containing 15 ml of glucose-grown cells (A600 = 1.47) or propylene-grown cells (A600 = 0.93) that had been transferred from 200-ml cultures grown in shake flasks. Closed symbols, assay bottles containing chloramphenicol (10 mg) and rifampin (5 mg); open symbols, assay bottles without chloramphenicol or rifampin present; circles, propylene-grown cells; squares, glucose-grown cells. (A) Propylene remaining; (B) epoxypropane remaining.

View larger version (60K): [in this window] [in a new window]   FIG. 4.   Gel electrophoretic analysis of propylene-induced polypeptides in N. corallina and comparison to purified epoxide carboxylase components (comp.) I and II from Xanthobacter strain Py2. Lane 1, molecular mass standards (2 µg each); lane 2, epoxide carboxylase component I from Xanthobacter strain Py2 (3 µg); lane 3, epoxide carboxylase component II from Xanthobacter strain Py2 (3 µg); lane 4, glucose-grown cell extract from Xanthobacter strain Py2 (25 µg); lane 5, propylene-grown cell extract from Xanthobacter strain Py2 (25 µg); lane 6, glucose-grown cell extract from N. corallina (25 µg); lane 7, propylene-grown cell extract from N. corallina (25 µg); lane 8, epoxide carboxylase components I (3 µg) and II (3 µg) from Xanthobacter strain Py2.

Immunoblot analysis of glucose- and propylene-grown N. corallina cell extracts. The polypeptides in glucose- and propylene-grown N. corallina cell extracts were analyzed by immunoblotting with polyclonal antibodies raised against purified epoxide carboxylase components I and II from Xanthobacter strain Py2 to determine whether there is any cross-antigenicity between these proteins. Antibodies raised against epoxide carboxylase component I from Xanthobacter strain Py2 cross-reacted weakly with a protein, present in cell extracts of propylene-grown N. corallina B276 cells, that migrated on SDS-PAGE with the same apparent molecular mass as the 42-kDa inducible polypeptide (Fig. 5A). Cross-reaction to a lesser degree was observed at this same position in cell extracts of glucose-grown N. corallina. Antibodies raised against purified epoxide carboxylase component II from Xanthobacter strain Py2 cross-reacted with a protein, present in both glucose- and propylene-grown N. corallina cell extracts, that migrated on SDS-PAGE with an apparent molecular mass of 37 kDa but did not cross-react with the 59-kDa inducible polypeptide (Fig. 5B).

View larger version (58K): [in this window] [in a new window]   FIG. 5.   Immunoblot analysis of glucose- and propylene-grown cell extracts of N. corallina. (A) Immunoblot prepared using antibodies raised against epoxide carboxylase component I from Xanthobacter strain Py2 as the probe. The arrow indicates the position of component I. Lanes 1 and 6, epoxide carboxylase component I from Xanthobacter strain Py2 (0.5 µg); lane 2, glucose-grown cell extract from Xanthobacter strain Py2 (15 µg); lane 3, propylene-grown cell extract from Xanthobacter strain Py2 (15 µg); lane 4, glucose-grown cell extract from N. corallina (15 µg); lane 5, propylene-grown cell extract from N. corallina (15 µg). (B) Immunoblot prepared using antibodies raised against epoxide carboxylase component II from Xanthobacter strain Py2 as the probe. The arrow indicates the position of component II. Lane 1, glucose-grown cell extract from N. corallina (15 µg); lane 2, propylene-grown cell extract from N. corallina (15 µg); lane 3, epoxide carboxylase component II from Xanthobacter strain Py2 (0.5 µg).

Inhibition of epoxide carboxylase activity in N. corallina by methylepoxypropane. As mentioned in the introduction, epoxide carboxylase from Xanthobacter Py2 is a multiprotein enzyme complex consisting of four separate proteins that are required for epoxide carboxylation or isomerization (3). Since epoxide carboxylation in cell extracts of N. corallina has cofactor requirements identical to those of the Xanthobacter strain Py2 epoxide carboxylase, it would be reasonable to speculate that N. corallina epoxide carboxylase functions as a multiprotein complex as well. One mechanistic probe that might prove useful in addressing this question is the substrate analog methylepoxypropane, which has previously been characterized as a time-dependent, irreversible inactivator of epoxide carboxylase from Xanthobacter strain Py2 (2). Methylepoxypropane differs from epoxypropane in the presence of a methyl group rather than a hydrogen substituent on the C-2 carbon atom. Epoxide carboxylation (or isomerization) requires the abstraction of hydrogen, possibly as a hydride, from the C-2 carbon and the formation of a carbonyl carbon at this center (21). Methylepoxypropane is proposed to undergo a covalent reaction with the catalytic, active-site-containing component of epoxide carboxylase but, due to the lack of an abstractable hydride, not to react further to form a product (2). The addition of epoxide carboxylase component I, but not that of component II, III, or IV, to methylepoxypropane-inactivated cell extracts of Xanthobacter strain Py2 restored epoxide carboxylase activity, demonstrating that component I is the specific target of inactivation and suggesting that it contains the epoxide binding and/or activation site(s) (3).

View larger version (21K): [in this window] [in a new window]   FIG. 6.   Concentration- and time-dependent inactivation of epoxide carboxylase in whole-cell suspensions of N. corallina by methylepoxypropane. Each assay mixture contained cell suspension (0.35 mg of protein) and CO2 and NaHCO3 (50 mM total). Assays were initiated by the addition of 2 µmol of epoxypropane. Symbols: , no methylepoxypropane; , 0.5 mM methylepoxypropane; , 1 mM methylepoxypropane; , 2 mM methylepoxypropane; , 4 mM methylepoxypropane.

Restoration of epoxide carboxylase activity in methylepoxypropane-treated N. corallina cell extract by Xanthobacter strain Py2 component I. As shown in Fig. 7A, the addition of Xanthobacter epoxide carboxylase component I to methylepoxypropane-inactivated N. corallina cell extract restored epoxide carboxylase activity in a concentration-dependent fashion. The addition of Xanthobacter epoxide carboxylase component II, III, or IV up to the highest amount of component I added (1.1 mg) had no effect on activity (data not shown). Importantly, purified component I did not display epoxide carboxylase activity alone (in the absence of methylepoxypropane-inactivated N. corallina cell extract) or in the presence of N. corallina glucose-grown cell extracts (data not shown). These results demonstrate that the combination of Xanthobacter epoxide carboxylase component I with active N. corallina epoxide carboxylase components provides a heterologous system capable of catalyzing epoxide carboxylation. These results also demonstrate that an N. corallina protein with functions similar to that of Xanthobacter component I is the specific target of methylepoxypropane inactivation.

View larger version (21K): [in this window] [in a new window]   FIG. 7.   Restoration of epoxide carboxylase activity in methylepoxypropane-treated cell extracts of N. corallina by addition of epoxide carboxylase component I from Xanthobacter strain Py2. Symbols: , assays performed with CO2 and NaHCO3 (60 mM); , assays performed without CO2 and NaHCO3. (A) Assays of cell extract (4.8 mg) prepared from methylepoxypropane-treated N. corallina; (B) assays of cell extract (5.4 mg) prepared from methylepoxypropane-treated Xanthobacter strain Py2.

Xanthobacter strain Py2 component II confers epoxide isomerase activity in cell extracts of N. corallina. The difference in the relative ratios of epoxide isomerization and carboxylation catalyzed by the Xanthobacter and N. corallina epoxide carboxylase systems was further investigated by individually adding each of the four purified Xanthobacter components to active N. corallina extracts and measuring the resultant rates of epoxide isomerase activity observed in the absence of CO₂. As shown in Fig. 8, the addition of component II resulted in a significantly higher rate of isomerase activity in the N. corallina cell extract. In contrast, the addition of component I, III, or IV did not stimulate isomerization to any noticeable degree.

View larger version (22K): [in this window] [in a new window]   FIG. 8.   Epoxide carboxylase component II from Xanthobacter strain Py2 confers epoxide isomerase activity in cell extracts of N. corallina. Assays were performed with cell extracts of propylene-grown N. corallina (7.5 mg) in the absence of CO2. Closed symbols, epoxypropane remaining; open symbols, acetone produced; inverted triangles, addition of epoxide carboxylase component I (0.8 mg); squares, addition of epoxide carboxylase component II (0.6 mg); circles, addition of epoxide carboxylase component III (0.5 mg); triangles, addition of epoxide carboxylase component IV (0.6 mg).

	DISCUSSION

Top Abstract Introduction Materials & Methods Results Discussion References

The results of the studies presented in this paper demonstrate that epoxide metabolism in N. corallina B276 proceeds by a CO₂-dependent reaction that forms -keto acids as products. This represents only the second example of biological epoxide carboxylation, the other being that of Xanthobacter strain Py2 (1, 18). These results demonstrate that epoxide carboxylation is not an isolated phenomenon and that it may, in fact, be the sole or predominant strategy for epoxide metabolism by the class of bacteria that grow with aliphatic alkenes as carbon and energy sources. N. corallina and Xanthobacter strain Py2 are phylogenetically distinct bacteria; N. corallina is a gram-positive actinomycete, while Xanthobacter strain Py2 is a gram-negative rod. The fact that two unrelated bacteria utilize the same pathway of aliphatic alkene and epoxide metabolism provides support for the idea that this is a fundamental microbial strategy for the utilization of these compounds.

The initial characterization of epoxide carboxylase from N. corallina suggests that it, like epoxide carboxylase from Xanthobacter strain Py2, functions as a multiprotein complex and requires an oxidant (NAD⁺) and a reductant (NADPH or DTT) as cofactors. In the purified epoxide carboxylase system from Xanthobacter strain Py2, the carboxylation of epoxides is coupled to the transhydrogenation of NAD⁺ and NADPH (equation 2). Presumably, epoxide carboxylation in N. corallina has the same reaction stoichiometry. These cofactor requirements, and the associated transhydrogenation, are unprecedented among the known carboxylases. The mechanism of epoxide carboxylation and the roles that the individual components play in the reaction are not well understood. Epoxide carboxylase component I from Xanthobacter strain Py2 has been proposed to play a key role in epoxide binding and activation, based on the specific inactivation of this component by the irreversible inactivator methylepoxypropane (2). Epoxide carboxylase component II has been proposed to function as an NADPH:disulfide oxidoreductase which generates a reduced thiol, possibly on component I, that serves as a nucleophile for attack on and ring opening of the epoxide substrate (19). It is unclear what role(s) epoxide carboxylase components III and IV play in the carboxylation reaction, but they may be involved in the reduction of NAD⁺ and/or the formation of the protein-protein complexes necessary for catalysis (3).

By analogy, proteins with functions similar to those of Xanthobacter epoxide carboxylase components I to IV are likely to be involved in epoxide carboxylation in N. corallina. Evidence for the involvement of a protein with component I activity was provided by the demonstration that Xanthobacter strain Py2 component I restored epoxide carboxylase activity when added to cell extracts of N. corallina that had been inactivated by methylepoxypropane (Fig. 7). Furthermore, induction of epoxide carboxylase activity in N. corallina led to the high-level expression of a polypeptide that migrated on SDS-PAGE with a molecular mass nearly identical to that of component I from Xanthobacter strain Py2 (Fig. 4) and that cross-reacts with polyclonal antibodies raised against component I. Together, these results suggest that a protein homologous to component I is required for epoxide carboxylation in N. corallina. A polypeptide with a molecular weight and level of expression similar to those of component II is present in cell extracts of propylene-grown N. corallina as well (Fig. 4), suggesting that this polypeptide may function in a role analogous to that of component II, i.e., in the oxidation of NADPH and the reduction of a disulfide. This polypeptide did not, however, cross-react to any detectable degree with polyclonal antibodies raised against Xanthobacter strain Py2 component II. At present, we have not obtained evidence that proteins analogous to components III and IV are present in N. corallina, although we suspect that such proteins are required. In Xanthobacter strain Py2, the levels of expression of components III and IV are significantly lower than those of components I and II, and they cannot be distinguished from the protein background on one-dimensional SDS-PAGE gels of cell extracts (3). Additional experiments, culminating with the purification and characterization of the necessary components from N. corallina, will be required to definitively define the proteins required by this multiprotein enzyme system.

While there are many apparent similarities between the epoxide carboxylase systems of Xanthobacter strain Py2 and N. corallina, there is one striking difference: the lower level of isomerase activity exhibited by N. corallina epoxide carboxylase when CO₂ is excluded from the assays. While epoxide isomerase activity was detected in cell extracts of N. corallina, the rate of isomerization was significantly lower (approximately five- to sevenfold) than that observed with cell extracts or purified components from Xanthobacter strain Py2. In Xanthobacter strain Py2, epoxide-to-ketone isomerization is apparently a fortuitous reaction of no physiological importance (16). The observation that isomerization can occur is, however, significant since it demonstrates that a reaction intermediate that can alternatively undergo carboxylation (with CO₂ present) or protonation (without CO₂ present) is generated. Since component I has been implicated in epoxide binding and activation in the Xanthobacter system (2, 3), and since the addition of purified component I from Xanthobacter strain Py2 restored epoxide carboxylase activity to methylepoxypropane-inactivated N. corallina extracts (Fig. 7A), it seemed reasonable to predict that differences in the respective component I proteins might be the determinant in the observed differences in epoxide carboxylation and isomerization in the two systems. It was therefore surprising to find that the addition of purified component II (the NADPH:disulfide oxidoreductase) from Xanthobacter strain Py2 to N. corallina extracts conferred an increase in epoxide isomerase activity to the level observed for the Xanthobacter system while components I, III, and IV had no stimulatory effect (Fig. 8). At present we have no clear explanation for these observations. Possibly, component II, as well as component I, contains a portion of the epoxide binding and activation sites or accepts an intermediate formed at some point during the reaction from another protein component. Differences in the properties of the respective component II proteins could thus result in increased or decreased rates of protonation when CO₂ is not present. The binding of component II to component I may also confer conformational changes that make the active site more or less accessible for proton abstraction from water or a general base. Once purified sources of the N. corallina epoxide carboxylase proteins are available, it will be interesting to compare their molecular properties to those of the Xanthobacter proteins and to investigate the ratios of carboxylase and isomerase activities when heterologous mixtures of the various components are combined. These studies should provide further insight into microbial strategies for the metabolism of xenobiotic compounds generated as intermediates in unsaturated-hydrocarbon metabolism.