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Disaccharides such as maltose, sucrose, and trehalose can serve as sole carbon and energy sources for Bacillus subtilis (12, 17, 33). In most cases, the accumulation of sugars is coupled with phosphate bond energy (20). Sucrose and trehalose utilization is dependent on the uptake of the sugars by their specific permeases, which are phosphoenolpyruvate-dependent phosphotransferase systems (PTS) correlated with the phosphorylation of the sugar (9, 12, 26). The phosphorylated sugars are further hydrolyzed in the cytoplasm by a specific phosphosucrase (17, 31) or phospho--1,1-glucosidase (10, 12). The latter reaction results in glucose-6-phosphate and glucose in a ratio of 1:1 from 1 mol of trehalose-6-phosphate (10). In a further reaction, the resulting glucose can serve as a substrate for an ATP-dependent glucose kinase (29). Internal glucose presumably also is produced by hydrolysis of other disaccharides such as maltose. Previous studies indicated that maltose is taken up by a non-PTS system, because uncouplers negatively affected maltose transport. This finding led to the conclusion that B. subtilis does not possess an enzyme II for maltose and that maltose uptake is a proton motive-driven process (33).

Amino acid sequence analysis. Newly available sequence data from the B. subtilis genome sequencing project make possible the identification of several genes coding for potential -glucosidases (15, 32). The predicted gene products deduced from the DNA sequence of one gene cluster (yvdE to yvdM) from 3,545.7 to 3,558.0 kb on the B. subtilis genomic map (Fig. 1) (32) showed amino acid similarities to proteins involved in maltose/maltodextrin utilization systems, which presumably belong to the ABC transporter family. Therefore, we have chosen this region for further investigation with regard to maltose utilization. The derived amino acid sequence from yvdL exhibits high similarities to several -glucosidases (32), indicating that the protein belongs to the glucosidase protein family. Therefore, and because of the results presented in this work, we designated the gene, predicted to encode a protein composed of 561 amino acids with a calculated molecular mass of 66 kDa and a pI of 4.98, malL.

View larger version (13K): [in this window] [in a new window]   FIG. 1.   The yvdE to yvdM region from 3,545 to 3,558 kb of B. subtilis. The yvdL gene (black arrow, now named malL) encoding the -glucosidase is depicted below the DNA. Restriction sites used for malL inactivation by the aphA3 cassette are listed. Potential transcription terminators as proposed in the SubtiList data bank (15, 32) are denoted at the ends of the DNA.

Expression of -glucosidase in B. subtilis. If an -glucosidase involved in maltose utilization exists in B. subtilis, one would expect it to be maltose inducible. Therefore, we investigated the dependence of para-nitrophenyl--D-glucopyranoside (PNPG) hydrolysis (PNPG is a synthetic substrate analogous for many -glucosidases) on the presence or absence of maltose by MalL in crude cell extracts. MalL activity was determined as previously described for the phospho--1,1-glucosidase TreA (10, 12) with C minimal medium containing all the required components (18) and sugars as mentioned; cells were harvested at an optical density at 600 nm (OD₆₀₀) as indicated (Fig. 2). As expected, high -glucosidase activity was detected only in cultures grown in C minimal medium containing 10 mM maltose, whereas only residual PNPG-hydrolyzing activity was present in cells grown without maltose (grown on 0.4% K-glutamate). The latter activity is about 15-fold lower than the maltose-induced level. These data lead to the conclusion that the -glucosidase expression (MalL; see below) is maltose inducible.

View larger version (20K): [in this window] [in a new window]   FIG. 2.   Growth phase-dependent MalL activity. B. subtilis strains were grown in minimal media containing 10 mM maltose. Aliquots were harvested at the indicated times, and MalL activity was determined with PNPG as a substrate and expressed in nanomoles of product formed minute1 milligram of crude protein extract1 for the wild-type () and the MalL strain MD193 (). The corresponding OD600s of the cultures are indicated for the wild-type () and the MalL strain MD193 (). The growth and MalL activities of strains MD192 and MD193 were identical.

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Cloning of the -glucosidase-encoding gene. The malL gene was cloned by amplification via PCR (19) with a set of appropriate primers. B. subtilis chromosomal DNA was used as a template with the oligonucleotides 5'-CGATGTGAAAGGAGAAGGATCCATGAGTG and 5'-GATATTCTGCAGTATCTGTTATCACTCCG, introducing a BamHI site 5' and a PstI site 3' to malL. The resulting 1,731-bp DNA fragment was ligated to the appropriate cloning sites of plasmid pQE-9 (21) after digestion with BamHI and PstI. In the resulting plasmid, pMalL, malL transcription is under an isopropyl--D-thiogalactopyranoside (IPTG)-inducible promoter and the gene for the -glucosidase is fused N-terminally in frame to the His-tag coding region of the plasmid. The encoded protein has a 12-residue N-terminal extension including the affinity tag (underlined): Met-Arg-Gly-Ser-His-His-His-His-His-His-Gly-Ser-Met .... Plasmid pMalL was transformed into Escherichia coli RB791 (2) and selected on Luria broth (25) supplemented with ampicillin (100 µg/ml). The addition of 2 mM IPTG to liquid cultures yielded an intense protein band in crude cell extracts migrating at the expected molecular mass of 66 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on 12% gels (16) which were stained with Coomassie blue R250. Thus, the construct pMalL results in the overexpression of MalL in E. coli.

Inactivation of MalL. To show that MalL is indeed involved in maltose utilization in B. subtilis and that malL encodes the observed maltose-inducible -glucosidase, we have constructed two malL mutations by insertional mutagenesis (Fig. 1 and Table 1) of the cloned gene in plasmid pMalL. In the first step, malL was inactivated on the plasmid, leading to plasmids pMalLK1 and pMalLK2, which were obtained by replacing an internal 549-bp HincII fragment of pMalL by a 1,494-bp SmaI/StuI DNA fragment of plasmid pDG792 (11) carrying the aphA3 gene (Fig. 1). Recombinants were selected in E. coli on Luria broth plates supplemented with ampicillin (100 µg/ml) and kanamycin (30 µg/ml). The orientation of the aphA3 cassette was determined by digestion with AvaI, whose cleavage site is asymmetrically located in aphA3. The resulting plasmid, pMalK1, carries aphA3 in the same orientation as malL, whereas in pMalLK2 aphA3 is oriented in the opposite direction. Strains MD192 and MD193 were constructed by transformation (14) of a 2,676-bp BamHI/PstI DNA fragment obtained from plasmids pMalLK1 and pMalLK2, respectively, in B. subtilis, followed by selection on kanamycin (30 µg/ml) for recombinants, which had arisen by a double crossover. The correct insertion in the chromosome of the integrants has been verified by PCR (see above) (19) with chromosomal DNA of B. subtilis wild type and the resulting strains MD192 and MD193 as templates. Both B. subtilis mutants were also analyzed for maltose-inducible -glucosidase activity. No significant enzymatic activity could be observed even in the maltose-induced state (Fig. 2).

Purification of the -glucosidase. E. coli RB791/pMalL was grown in 100 ml of Luria broth at 37°C. Expression of malL was induced by the addition of 2 mM IPTG when the culture reached an OD₆₀₀ of 0.5. Growth was allowed to proceed for another 5 h, and the cells were harvested by centrifugation at 5,000 × g. The resulting cell pellet was washed once in lysis buffer (25 mM imidazole [pH 7.0], 10 mM potassium chloride, 1 mM magnesium chloride, 1 mM calcium chloride, 2 mM 1,4-dithiothreitol), resuspended in the same buffer, and frozen at 70°C. Frozen cell pellets were thawed on ice and sonicated six times for 30 s each at 40 W with 30-s intervals with a Labsonic U sonicator (B. Braun, Melsungen, Germany). After centrifugation for 30 min at 40,000 × g in a Sorvall SS34 rotor, overproduced soluble MalL was present in the supernatant. The crude extract was passed over a 1-ml Ni²⁺-loaded HiTrap chelating column (Pharmacia, Freiburg, Germany) which had been equilibrated with lysis buffer with a Pharmacia Äkta purifier apparatus. The column was washed with 5 column volumes of lysis buffer until the absorption at 280 nm showed a stable baseline, and protein was eluted with a 10-ml linear gradient between lysis buffer and lysis buffer containing 500 mM imidazole at a flow rate of 1 ml/min (Fig. 3). Fractions (2 ml for column washing and 0.5 ml for gradient elution) were collected. The fusion protein typically eluted at about 250 mM imidazole. During protein purification, we analyzed the total and specific MalL activities from each purification step and the enrichment of MalL protein by SDS-PAGE (Fig. 3). The enrichment of MalL activity was calculated to be 45-fold. A 100-ml culture yielded about 2.4 mg of pure protein. Purified MalL was reasonably stable when stored in elution buffer at 4°C for at least 4 weeks.

View larger version (30K): [in this window] [in a new window]   FIG. 3.   Elution profile in Ni2+ HiTrap chelating column and purification of MalL. Protein absorption at 280 nm (solid line) and the imidazole gradient as the percentage of buffer B (dashed line) are presented as a function of the column volume (in milliliters). Analysis of selected fractions (indicated by numbers on the elution profile) on SDS-PAGE is shown in the insert. Pure MalL is in fraction 4. Molecular mass standards with the indicated sizes in kilodaltons (kD) are shown in lane st. mAU, milli-absorption units.

Physical properties of MalL activity. To compare the stability of MalL with that of other -glucosidases, we examined its activity under the influence of different parameters. A standard MalL activity test was used (containing 3 µg of purified protein per ml at 25°C) to determine the optimum pH. The pH of the reaction buffer was varied with either HCl or NaOH or supplemented with different concentrations of NaCl and KCl. With PNPG as the substrate, the pH profile was essentially symmetrical with optimal activity at pH 7.0. At pHs 5.75 and 8.0, the activity was reduced to 50%. MalL activity was inhibited by salt and exhibited 50% inhibition at 500 mM NaCl and 40% inhibition at 1 M KCl. These findings differ from the previously described effects of salt on the homologous phospho--1,1-glucosidase TreA, whose enzymatic activity is stimulated up to 10-fold at the appropriate salt concentrations (10).

Substrate specificity and kinetic parameters of MalL. The phenotype of the malL mutants and the analysis of the amino acid sequence of MalL suggested that the gene encodes an -glucosidase. However, the enzyme specificity cannot be determined definitively from the sequence analysis. Therefore, the specific activity of purified MalL was monitored by determining the release of free glucose after disaccharide hydrolysis by coupling the enzymatic activity with hexokinase and glucose-6-phosphate dehydrogenase following the method described by Seno and Charter (28). For this purpose, we used the glucose determination kit HK10 from Sigma (Deisenhofen, Germany) with modifications as described by the manufacturer. The reaction mixture contained 50 mM Tris-HCl, (pH 7.0), 25 mM MgCl₂, 0.5 mM NAD, 1 mM ATP, and 0.8 U of glucose-6-phosphate dehydrogenase and was incubated at 25°C. Glucose-6-phosphate dehydrogenase activity was assayed with NAD as the cofactor by monitoring the change in OD₃₄₀. Kinetic parameters were determined with 3 µg of purified MalL in a 1-ml reaction volume. Using substrates at 8 mM allowed us to conclude initially that in addition to PNPG, MalL hydrolyzes sucrose, maltose, and isomaltose, with specific activities of 4.47, 1.7, and 2.02 µmol of glucose per min per mg of protein, respectively. Under these assay conditions, no hydrolysis of trehalose, ortho-nitrophenyl galactopyranoside (ONPG), or melibiose and only weak lactose hydrolysis (0.04 µmol/min/mg) were observed. Analysis of the K_m and V_max of MalL for different substrates showed that MalL has 22- to 76-fold-higher affinities for isomaltose and maltose than for sucrose (Table 2). However, the V_max for sucrose is faster than that for cleavage of isomaltose and maltose. The K_m for PNPG is similar to that for maltose, but the V_max for cleavage of PNPG is up to 100-fold lower than that observed for various disaccharides. From these data, it can be concluded that MalL efficiently hydrolyzes -glycosidic 1,4- and 1,6-disaccharides but not -1,1- or -glycosidic bonds. The enzyme must also discriminate between galactosides and glucosides, because no hydrolysis of melibiose (-1,6-galactopyranosyl--D-glucose) was detected. Therefore, we categorize this enzyme as an oligo-1,4-1,6--glucosidase. By virtue of its ability to hydrolyze sucrose and PNPG, this enzyme may be assigned the general designation oligo--glucosidase.