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Journal of Bacteriology, June 1999, p. 3368-3374, Vol. 181, No. 11
Unité des Agents Antibactériens,
Institut Pasteur, 75724 Paris Cedex 15, France
Received 19 October 1998/Accepted 3 April 1999
We determined the sequence and genetic organization of plasmid
pIP823, which contains the dfrD gene; dfrD
confers high-level trimethoprim resistance to Listeria
monocytogenes BM4293 by synthesis of dihydrofolate reductase type
S2. pIP823 possessed all the features of the pUB110/pC194 plasmid
family, whose members replicate by the rolling-circle mechanism. The
rep gene encoded a protein identical to RepU, the protein
required for initiation of the replication of plasmids pTB913 from a
thermophilic Bacillus sp. and pUB110 from
Staphylococcus aureus. The mob gene encoded a
protein with a high degree of amino acid identity with the Mob proteins
involved in conjugative mobilization and interplasmidic recombination
of pTB913 and pUB110. The host range of pIP823 was broad and included L. monocytogenes, Enterococcus faecalis,
S. aureus, Bacillus subtilis, and
Escherichia coli. In all these species, pIP823 replicated by generating single-stranded DNA and was stable. Conjugative mobilization of pIP823 was obtained by self-transferable plasmids between L. monocytogenes and E. faecalis,
between L. monocytogenes and E. coli, and
between strains of E. coli, and by the streptococcal conjugative transposon Tn1545 from L. monocytogenes to E. faecalis, and from L. monocytogenes and E. faecalis to E. coli. These data indicate that the gene flux observed in nature
from gram-positive to gram-negative bacteria can occur by conjugative
mobilization. Our results suggest that dissemination of trimethoprim
resistance in Listeria spp. and acquisition of other
antibiotic resistance determinants in this species can be anticipated.
Most small multicopy plasmids from
gram-positive bacteria replicate by an asymmetric rolling-circle (RC)
mechanism, producing a single-stranded DNA (ssDNA)
intermediate. Recently, plasmids which replicate by the same
mechanism have been detected in gram-negative bacteria (11).
Three structural modules are required for RC replication: (i) the
rep gene, which encodes the Rep protein involved in
initiation of plasmid replication; (ii) the double-stranded origin
(dso or plus origin); and (iii) the single-stranded origin (sso or minus origin) (12). The Rep protein
introduces a strand- and site-specific nick in supercoiled plasmid DNA
at the dso domain (21, 32). Replication proceeds
around the whole plasmid, until the entire dso is
synthesized and the parental plus strand is released as an ssDNA
intermediate. The last stage of RC replication involves the conversion
of plasmid ssDNA to double-stranded DNA (dsDNA) by synthesis of the
lagging strand (8), initiated at the sso domain.
On the basis of homology in the DNA sequence and the genetic
organization of the replication region, these plasmids have been
classified into four families represented by pUB110/pC194, pT181,
pLS1/pE194, and pSN2 (12). Some of these plasmids have a
broad host range and can replicate in both gram-positive and gram-negative bacteria (11).
Frequently, RC replication plasmids contain two other structural
elements: (i) a mob or pre gene, which encodes a
protein involved in plasmid conjugative mobilization and site-specific interplasmidic recombination, and (ii) an antibiotic resistance determinant (12, 24). Mobilization of these small
non-self-transferable plasmids by conjugative plasmids or transposons
has been reported for gram-positive bacterial genera such as
Bacillus and Staphylococcus (24, 25,
28). The mobilization process seems to occur by donation, as has
been observed in gram-negative bacteria: the trans-acting
Mob protein produces a nick in the plasmid DNA at the RSA,
site which functions as a cis-acting origin of transfer (24). The RC replication plasmids are highly recombinogenic, a feature that may favor their intra- and intergeneric dissemination (12). Both the simplicity of the replication mechanism and
the dependence on host functions for their replication may explain the
widespread interspecies transfer of plasmids by the RC replication mode
(11). RC replication plasmids conferring resistance to tetracycline, chloramphenicol, neomycin, bleomycin, erythromycin, and
kanamycin have been reported (11).
Listeria monocytogenes is a ubiquitous gram-positive,
rod-shaped species causing perinatal infections, meningitis,
meningoencephalitis, and septicemia (26). Penicillin G or
ampicillin, alone or in combination with gentamicin or cotrimoxazole,
constitutes standard treatment for listerial infections
(13). It has been shown that self-transferable plasmids and
conjugative transposons can confer multiple-antibiotic resistance
in L. monocytogenes (4, 6). Several studies have demonstrated that antibiotic resistance in L. monocytogenes emerged recently worldwide, probably
as the result of acquisition of these two types of mobile genetic
elements from enterococci (4, 6).
We previously isolated plasmid pIP823, which carries the
dfrD gene; this gene confers high-level trimethoprim
resistance to L. monocytogenes BM4293 (3,
4). In this study, we report the entire sequence and structural
organization of pIP823, which belongs to the pUB110/pC194 family of RC
replication plasmids. Conjugative mobilization of pIP823 is shown
to be mediated by self-transferable plasmids between L. monocytogenes and Enterococcus faecalis, between
Escherichia coli and L. monocytogenes, and
among strains of E. coli, and by the conjugative
transposon Tn1545 from L. monocytogenes to
E. faecalis and from L. monocytogenes and E. faecalis to
E. coli.
Bacterial strains, plasmids, transposon, and growth
conditions.
The main characteristics of the bacterial strains,
plasmids, and transposon used in this study are listed in Table
1. Bacillus subtilis BM4150;
E. coli DH5
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Conjugative Mobilization of the Rolling-Circle Plasmid pIP823
from Listeria monocytogenes BM4293 among Gram-Positive
and Gram-Negative Bacteria
ABSTRACT
Top
Abstract
Introduction
Materials and Methods
References
INTRODUCTION
Top
Abstract
Introduction
Materials and Methods
References
MATERIALS AND METHODS
Top
Abstract
Introduction
Materials and Methods
References
, HB101, and K802N; E. faecalis JH2-2 and BM4110; L. monocytogenes
BM4293, EGDSmR, and LO17RF; and Staphylococcus aureus
80CR5RF, 80CR5Str, and RN4220 were used as recipients in conjugation
experiments. E. coli DH5 and SM10, E. faecalis JH2-2, L. monocytogenes LO17RF, and
S. aureus RN4220 were used in electrotransformation experiments. B. subtilis 168 and E. coli HB101 and JM83 were used in transformation experiments.
Bacteria were grown in brain-heart infusion broth (Difco Laboratories,
Detroit, Mich.) and on Mueller-Hinton agar (Sanofi Diagnostics Pasteur,
Marnes-la-Coquette, France) at 37°C.
TABLE 1.
Bacterial strains, plasmids, and transposons
Preparation of DNA, DNA techniques, and sequencing.
Total
DNA was prepared as described previously (4). Plasmid pIP823
DNA was purified according to a modification of the alkaline-sodium
dodecyl sulfate extraction procedure (4). Recombinant DNA
techniques, including cleavage of DNA with restriction endonucleases and ligation with T4 DNA ligase, were performed by standard methods (27). Sequencing reactions were performed on both strands of DNA by the dideoxynucleotide chain-termination method (27)
with a Sequenase version 2.0 kit, modified T7 DNA polymerase, and
[-35S]dATP as recommended by the manufacturers.
Computer analysis of sequence data. DNA and amino acid sequence analyses were performed with Genetics Computer Group programs (9).
Transformation and electrotransformation. Transformation was performed as described previously (17, 27). Electrotransformation was carried out by using a Gene Pulser apparatus (Bio-Rad Laboratories, Richmond, Calif.) (18, 27). The antibiotics and the concentrations used for selection of the transformants were as follows: ampicillin, 100 µg/ml; fusidic acid, 20 µg/ml; nalidixic acid, 25 µg/ml; rifampin, 20 µg/ml; streptomycin, 200 µg/ml; and trimethoprim, 5 µg/ml.
Mating experiments.
Filter matings were performed as
described previously (4, 34, 35). Transfer frequencies were
expressed as the number of transconjugants per donor CFU after the
mating period. The antibiotics and the concentrations used for
selection of transconjugants were as follows: chloramphenicol, 15 µg/ml; erythromycin, 10 µg/ml; fusidic acid, 20 µg/ml; kanamycin,
20 µg/ml for E. coli and L. monocytogenes and 1,000 µg/ml for E. faecalis;
nalidixic acid, 25 µg/ml for E. coli DH5 and 50 µg/ml for E. coli K802N; rifampin, 20 µg/ml;
streptomycin, 200 µg/ml for E. coli HB101 and 500 µg/ml for B. subtilis BM4150, E. faecalis BM4110, L. monocytogenes EGDSmR, and
S. aureus 80CR5Str; tetracycline, 10 µg/ml; and
trimethoprim, 5 µg/ml.
PCR amplification.
The PCR mixture was submitted to a
denaturation step (3 min at 94°C), followed by 35 cycles of
amplification (50 s of denaturation at 94°C, 50 s of annealing
at 50°C, and 1 min 30 s of elongation at 72°C). The primers
used were D1 (5' ATTTCTTTAATTGTTGC 3'OH), D2 (5'
GACATAAGGCAAGAACA 3'OH), M1 (5' ATTACTTGTCACGTCTG 3'OH), M2 (5' TTCTTTTGCTCGATCCC 3'OH), R1 (5'
CGAGTCTTTCTATTCTT 3'OH), and R2 (5' TTTATTGTCACTTCCGT 3'OH).
The amplified DNA fragments were separated by agarose gel
electrophoresis and transferred to Nytran membranes (27).
Plasmid pIP823 DNA was labeled with [-32P]dCTP with a
Nick-Translation kit according to the instructions of the manufacturer.
Prehybridization and hybridization under stringent conditions were
carried out as described previously (4, 27).
Detection of pIP823 ssDNA. Strains harboring pIP823 were grown to mid-logarithmic phase and treated for 2 h with (i) no additions, (ii) erythromycin (100 µg/ml), or (iii) erythromycin plus rifampin (100 µg/ml each). Total DNA was prepared (31), electrophoresed on a 1% agarose gel, and transferred to a Nytran membrane by diffusion with 20× SSC (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate) according to the instructions of the manufacturer. Membranes were prehybridized and hybridized at 68°C with radiolabeled pIP823 dsDNA as described previously (27).
Characterization of ssDNA and determination of plasmid pIP823 copy number. Replication intermediates of RC replication plasmids can be detected in crude extracts from host cells as a band that migrates faster than supercoiled plasmid DNA during electrophoresis of agarose gels containing ethidium bromide (33). If pIP823 replicates by an RC mechanism, then ssDNA intermediates should accumulate in the various bacterial hosts. Erythromycin inhibits protein synthesis and thus prevents initiation of plasmid replication. In the presence of erythromycin, ssDNA molecules are converted to dsDNA by the host RNA polymerases that are still efficient. Since rifampin inhibits the activity of RNA polymerases in the presence of both erythromycin and rifampin, the conversion of ssDNA to dsDNA is inhibited, resulting in the accumulation of ssDNA molecules. Whole-cell lysates were prepared as described previously (33). Appropriate dilutions of DNA were run on 1% agarose gels containing 0.5 µg of ethidium bromide per ml. The plasmid copy number was estimated by comparing the intensity of plasmid DNA in each strain with those of plasmids pSC101, pBR322, and pUC18.
Analysis of plasmid pIP823 segregational stability. Bacterial strains harboring plasmid pIP823 were grown in Mueller-Hinton medium containing trimethoprim (5 µg/ml). Overnight saturated cultures were used to inoculate the same medium without antibiotic and were incubated at 37°C. Ten successive transfers (approximately 100 generations) were performed in antibiotic-free medium. The cells were then plated on nonselective Mueller-Hinton agar after serial dilutions. Approximately 100 colonies from each sample were transferred onto nonselective and selective agar plates to determine the proportion of bacteria containing plasmid DNA.
Enzymes and chemicals.
Restriction endonucleases, T4 DNA
ligase, T7 DNA polymerase, and Taq DNA polymerase (Pharmacia
Biotech SA, Saint Quentin en Yvelines, France) were used according to
the recommendations of the manufacturer. Lysozyme and lysostaphin were
obtained from Sigma Chemical Co. (St. Louis, Mo.) and Applied
Microbiology Inc. (Tarrytown, N.Y.), respectively. A Sequenase version
2.0 DNA sequencing kit was provided by United States Biochemical
Corporation (Cleveland, Ohio). The Nick-Translation reagent kit was
supplied by Amersham International plc (Little Chalfont,
Buckinghamshire, England). Nytran NY13N was obtained from Schleicher
and Schuell (Duren, Germany). [-35S]dATP and
[-32P]dCTP were purchased from Amersham Radiochemical
Center (Amersham, England). The 1-kb-ladder molecular weight marker and
the supercoiled-DNA marker were obtained from Life Technologies Gibco
BRL (Eragny, France). The following antibiotics were provided by the
indicated laboratories: ampicillin, Panpharma (Fougères, France);
chloramphenicol, Roussel-Uclaf (Romainville, France);
erythromycin, Abbott (Rungis, France); fusidic acid, Leo
(Montigny-Le-Bretonneux, France); kanamycin, Bristol (Paris-La
Défense, France); nalidixic acid, Sanofi Winthrop (Gentilly,
France); rifampin, Marion Merrel SA (Levallois-Perret, France);
streptomycin, Diamant (Puteaux, France); tetracycline, Rhône-Poulenc Rorer (Vitry-sur-Seine, France); and trimethoprim, Roche (Fontenay-sous-Bois, France).
Nucleotide sequence accession number. The nucleotide sequence of plasmid pIP823 has been deposited in the GenBank database under accession no. u0997.
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RESULTS AND DISCUSSION |
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Nucleotide sequence of pIP823. Plasmid pIP823 DNA was digested with HindIII, cloned into HindIII-linearized pUC18, and transformed into E. coli JM83. The resulting plasmid, pAT459, conferred trimethoprim resistance to this new host. The sequence of the pAT459 insert of 3,712 bp was determined on both strands (Fig. 1). Analysis of the sequence revealed the presence of two open reading frames (ORFs) and of a third ORF, which appeared to be disrupted at the HindIII cloning site. In order to confirm this assumption and prove that no segment of pIP823 was lost during cloning, two primers (5' ATTACTTGTCACGTCTG 3'OH) and (5' TTTCTTTTGCTCGATCC 3'OH) were designed to be complementary to the pAT459 insert at 261 bp upstream and 245 bp downstream from the HindIII site while allowing amplification of a 514-bp fragment with pIP823 DNA as a template. The sequence of the amplification product corresponded to that predicted for the insert of pAT459 circularized at the HindIII site. The third ORF started 735 bp upstream from the HindIII site in the 3' part of the insert and ended at 504 bp downstream from this site in the 5' part of the insert. Thus, the 3,712-bp HindIII insert of pAT459 corresponded to pIP823 linearized at this site.
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pIP823 organization and similarities to RC plasmids.
The first
ORF, from positions 2159 to 2675, corresponds to the dfrD
gene, which encodes trimethoprim-resistant dihydrofolate reductase type
S2 (S2DHFR) (Fig. 1) (3). The second ORF, from positions 970 to 1975, corresponds to the Rep protein with motifs characteristic of
RC replication proteins (Fig. 1). The sequence of Rep was found to be
identical to that of RepU of plasmids pUB110 from S. aureus and pTB913 from a thermophilic Bacillus sp.
(19, 23), which belong to the pC194/pUB110/pBC16 RC
replication plasmid family (12). The degree of amino acid
identity of the deduced Rep protein of pIP823 with Rep proteins of the
other plasmids of this family ranged from 31.8 to 52.6%. The predicted
Rep protein of pIP823 contains a set of three conserved sequence motifs
that are typical of initiator Rep proteins of RC replication replicons related to the E. coli bacteriophage 
X174, and this
Rep is closely related to those of the pC194 family (12). A
sequence similar to that of dso of the RC replication
plasmids was detected 449 bp upstream from the rep gene.
This sequence, from positions 796 to 821, differs by a single
nucleotide from the dso of pTB913 (Fig. 1). We also
identified in plasmid pIP823, between nucleotides 2775 and 3046 upstream from the mob gene, a conversion signal identical to
that of the palU-type sso of pTB913
(8). Therefore, it is likely that both plasmids have the
same mechanism of replication initiation and termination. The product
of the third ORF, from positions 3206 to 742, corresponds to a Mob
protein possessing 97.6% identity with the Pre protein of plasmid
pTB913, 90.4% identity with that of pTB53, but only 60.5%
identity with the Mob protein of pUB110. Proteins Pre and Mob are
involved in site-specific recombination and conjugative mobilization of
these plasmids (23). The high level of similarity between
the product of the ORF of pIP823 and the Pre and Mob proteins of pTB913
and pUB110 makes it likely that this ORF is responsible for
recombination or mobilization of pIP823. A sequence similar to that of
the RSA site of pUB110 was identified between positions
3127 and 3150 upstream from the mob gene. The
RSA site of pUB110 has been shown to be involved in
conjugative mobilization, and it has been suggested that the potential
palindromic structure of this site might have a function similar
to that of the origins of transfer of gram-negative bacteria (24, 28).
Host range, stability, and copy number of pIP823.
Plasmid
pIP823 was introduced by transformation into B. subtilis 168, E. coli HB101, E. faecalis JH2-2, L. monocytogenes LO17RF, S. aureus RN4220, and E. coli DH5.
The plasmid conferred high-level resistance to trimethoprim (MICs
from 1,028 to 2,056 µg/ml) and was stably maintained in the
various hosts, with 100% of the cells retaining the plasmid after
growth for ca. 100 generations in the absence of trimethoprim. The copy
number of pIP823 was estimated to vary from 2 to 20 copies per
chromosome equivalent, depending on the host.
Identification of ssDNA pIP823 replication intermediates. Numerous RC replication plasmids from gram-positive bacteria accumulate ssDNA molecules (32, 33). We screened for the presence of pIP823 ssDNA intermediates in lysates of L. monocytogenes BM4293, E. faecalis JH2-2 (pIP823), S. aureus RN4220 (pIP823), B. subtilis 168 (pIP823), and E. coli HB101 (pIP823). As shown in Fig. 2 (lane 2), in the absence of rifampin and erythromycin L. monocytogenes BM4293 accumulated ssDNA molecules, suggesting that sso of pIP823 is not efficient in this host. In the presence of erythromycin (lane 3), ssDNA was converted to dsDNA. In the presence of erythromycin and rifampin, ssDNA molecules accumulated in L. monocytogenes BM4293 (lane 4) but also in B. subtilis (pIP823) (lane 7), S. aureus (pIP823) (lane 6), and E. coli HB101 (pIP823) (lane 8), confirming that conversion of ssDNA to dsDNA is dependent on RNA polymerase activity in these hosts. Under similar culture conditions, multimer ssDNA intermediates were detected in E. faecalis JH2-2 (pIP823) lysates (lane 5). In contrast to pUB110, which does not replicate in E. coli, pIP823 replicated and accumulated ssDNA in this species. The sso of pIP823 was RNA polymerase dependent (Fig. 2 and data not shown). Usually sso shows activity only in a limited number of hosts (11, 12). The amount of ssDNA molecules accumulated during RC replication is plasmid and host dependent since the conversion rate of ssDNA to dsDNA depends on the efficiency of the host machinery to recognize a given plasmid's sso (36).
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pIP823 was not self-transferable by conjugation.
In a previous
study, we proposed that transferable plasmid-mediated
multiple-antibiotic resistance and transposon-borne tetracycline resistance in L. monocytogenes result from acquisition
of these genetic elements from enterococci (4). Based on the
identity of dfrD of L. monocytogenes BM4293
with the corresponding gene in Staphylococcus haemolyticus,
we also suggested that trimethoprim resistance in Listeria
may have originated in Staphylococcus (3). The
data obtained since then show that trimethoprim resistance in strain
BM4293 is associated with an RC replicon, a plasmid type common in
staphylococci. It is thus possible that trimethoprim resistance is due
to plasmid transfer, and we therefore studied the transferability of
pIP823. As expected, attempts to transfer pIP823 by conjugation
between L. monocytogenes, E. faecalis,
S. aureus, B. subtilis, and
E. coli were unsuccessful, suggesting that pIP823 was
not self-transferable (data not shown). The ability of pIP823 to be
mobilized by various conjugative plasmids and transposons between these
gram-positive and gram-negative species in which it replicates was then
studied (Table 2).
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Conjugative mobilization of pIP823 by self-transferable
plasmids from gram-positive bacteria.
The broad-host-range
enterococcal plasmid pAM
1 was introduced by conjugation from
E. faecalis BM4110 (pAM1) into L. monocytogenes BM4293 and S. aureus RN4220
(pIP823) at mobilization frequencies of 3.7 × 10
2 and 9.3 × 106, respectively.
Transfer by conjugative mobilization of plasmid pIP823 was
obtained from the L. monocytogenes BM4293
(pAM1) donor to L. monocytogenes LO17RF and
E. faecalis JH2-2 recipients. From transconjugant
L. monocytogenes LO17RF (pAM1 plus pIP823),
pIP823 could be mobilized to L. monocytogenes
EGDSmR and E. faecalis BM4110, and from transconjugant
E. faecalis JH2-2 (pAM1 plus pIP823), it could be
mobilized to L. monocytogenes EGDSmR. The resistance
phenotypes of 100 transconjugants from each mobilization experiment
indicated that pAM1 was cotransferred in all cases. Mobilization of
pIP823 by pAM1 was not obtained with (i) L. monocytogenes BM4293 (pAM1) as a donor and the other
gram-positive bacteria or E. coli as recipients or (ii)
an S. aureus donor and either L. monocytogenes or S. aureus recipients.
Nevertheless, pAM1 could be conjugated from L. monocytogenes BM4293 to S. aureus 80CR5RF and from
S. aureus RN4220 to L. monocytogenes
LO17RF at frequencies of 105 and 2 × 109, respectively. No mobilization of pIP823 by pAM1
could be detected from L. monocytogenes to
S. aureus, B. subtilis, and
E. coli (Table 2). The gram-negative and gram-positive
shuttle plasmid pAT191 contains the origin of replication and the
transfer functions of pAM1, the replication origin of pBR322, and
the transfer origin of IncP plasmid RK2 from gram-negative bacteria
(33). This bireplicon, which conjugates from E. faecalis to E. coli, was introduced by conjugation
from E. faecalis BM4110 (pAT191) to L. monocytogenes BM4293 and E. faecalis JH2-2
(pIP823) with frequencies of 1.8 × 103 and
1.7 × 102, respectively. Conjugative mobilization
of pIP823 by pAT191 was obtained from L. monocytogenes BM4293 and E. faecalis JH2-2
(pIP823) donors to E. faecalis BM4110 and E. coli HB101 recipients. Plasmid pIP823 could not be mobilized by
conjugation by pAT191 from E. coli HB101 to
E. coli K802N, L. monocytogenes LO17RF,
or E. faecalis JH2-2.
Conjugative mobilization of pIP823 by the streptococcal conjugative transposon Tn1545. Plasmid pIP823 was introduced by electrotransformation into E. faecalis JH2-2::Tn1545 and L. monocytogenes LO17RF::Tn1545. The plasmid was successfully mobilized by conjugative transposon Tn1545 from L. monocytogenes LO17RF::Tn1545 (pIP823) to L. monocytogenes EGDSmR and E. coli HB101 and from E. faecalis JH2-2::Tn1545 (pIP823) to L. monocytogenes EGDSmR (Table 2). None of the transconjugants contained Tn1545. No conjugative mobilization of pIP823 by Tn1545 from L. monocytogenes LO17RF::Tn1545 (pIP823) to E. faecalis BM4110 and E. coli HB101 and from E. faecalis JH2-2::Tn1545 (pIP823) to E. faecalis BM4110 was obtained.
Conjugative mobilization of pIP823 by self-transferable
plasmids from gram-negative bacteria.
Plasmids RP4, R64, and
pIP55-1 were introduced by conjugation into E. coli
HB101 (pIP823) with frequencies of 1.4 × 104,
2.2 × 104, and 1.4 × 104,
respectively. Plasmid pIP823 could be mobilized from E. coli HB101 to E. coli K802N by the three plasmids
(Table 2). In the experiment with RP4 as a helper plasmid, of the 100 transconjugants analyzed, all had acquired both pIP823 and RP4 whereas
R64 was cotransferred to only 32% of the 100 transconjugants studied. Plasmid pIP823 was mobilizable from E. coli K802N
(RP4 plus pIP823) to L. monocytogenes EGDSmR but
not to E. faecalis BM4110 or from E. coli K802N (RP64 plus pIP823) or K802N (pIP55-1 plus pIP823) to
L. monocytogenes EGDSmR and E. faecalis BM4110.
(pIP823) at frequencies of 1.2 × 104
and 1.1 × 101, respectively. Plasmid pIP823
was mobilized from E. coli DH5 (pOX38-Km plus
pIP823) to E. coli HB101 but not from E. coli HB101 (pOX38-Km plus pIP823) to E. coli
DH5 and L. monocytogenes LO17RF (Table 2).
E. coli SM10, which contains the IncP group
transfer functions of RK2 integrated into the chromosome, was
electrotransformed with pIP823 DNA. Conjugative mobilization was
obtained from E. coli SM10 (pIP823) to
E. coli K802N and L. monocytogenes
EGDSmR but not to E. faecalis BM4110. This result
indicates that mobilization of pIP823 by RK2 occurs in the absence of cointegration.
The presence of pIP823 in transconjugants from all of the mobilization
experiments described above was examined by amplification of total DNA
with oligonucleotides specific for rep, dfrD, and mob followed by hybridization of the PCR products obtained
with pIP823 DNA as a probe (data not shown). For each strain, fragments of the expected size which hybridized with the pIP823 probe were obtained. Small nonconjugative plasmids have been shown to be mobilized
by conjugative plasmids among gram-positive bacteria. The S. aureus plasmid pC221 is mobilized by pG01 (25), whereas pLS20 can mediate transfer of pBC16 and pUB110 (15).
The nonconjugative streptococcal plasmid pMV158 can be mobilized by
both pAM1 and pIP501 (24). The mechanism of
mobilization of relaxable plasmids in S. aureus appears
to be analogous to that of mobilization by donation in gram-negative
bacteria (25). Transfer by mobilization of pC194 and pUB110
by the conjugative transposons Tn925 and Tn916 between Bacillus species has been described previously
(20, 29). In contrast to pIP823, pTB913 was reported to be
nonmobilizable (22). This is surprising in view of the
similarities between the Mob proteins and the RSA sites of
the two plasmids. It would be interesting to examine transfer of pTB913
in the context developed here.
In conclusion, we have characterized the natural RC plasmid pIP823 from
L. monocytogenes BM4293, which bears the gene that encodes trimethoprim-resistant S2DHFR. This plasmid belongs to the
pC194/pUB110 family of RC replication plasmids. The similarities with
pUB110 from Staphylococcus and pTB913 from
Bacillus include all the features essential for this
replication mode: (i) a dso sequence for initiation of
plus-strand synthesis, (ii) a rep lication protein, and (iii) an
sso sequence for initiation of minus-strand synthesis.
Rep of pIP823 was identical to those of pTB913 and pUB110. Plasmid
pIP823 bears the gene that encodes a Mob protein showing a high degree
of identity with Mob of pTB913 and possesses a potential
RSA site closely related to those of members of the pC194/pUB110 RC replication plasmid family. Plasmid pIP823 has an
exceptionally broad host range of replication since it is maintained stably in L. monocytogenes, E. faecalis, S. aureus, B. subtilis, and E. coli, in which plasmid ssDNA intermediates
accumulated. One of the main features of RC plasmids is their
promiscuity, which is attributed to replication functions that appear
to be active in a large variety of hosts. Other functions such as
transferability by mobilization, cassette exchange, and recombination
may also contribute to dissemination of these plasmids. The
bleo and knt genes of pTB913 and pUB110 confer
resistance to bleomycin and kanamycin, respectively. Plasmid pIP823 may
have diverged from pTB913 by replacement of the bleomycin and kanamycin
resistance cassettes by the trimethoprim resistance determinant at the
recombination-specific site RSA (Fig. 1); the reverse
filiation is as likely. However, it is noteworthy that pTB913 has been
detected only in an integrated state as part of plasmid pTB19 of
Bacillus. The high degree of similarity between the RC
plasmids indicates easy horizontal transfer of these replicons between
gram-positive cocci and bacilli, including Listeria. In
addition, we have demonstrated conjugative mobilization of pIP823 by
plasmids and a transposon between L. monocytogenes, E. faecalis, and E. coli. These
findings raise, once more, the question of the so-called genetic
barrier between gram-positive and gram-negative bacteria
(6). Based on its properties, pIP823 might be
attractive for the development of new broad-host-range cloning and
expression gram-positive-gram-negative shuttle vectors. Characterization of pIP823 is also of interest in view of the possible
dissemination of trimethoprim resistance in Listeria.
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ACKNOWLEDGMENTS |
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E.C. was a recipient of a Pasteur-Weizmann fellowship during part of this work.
We are grateful to A. Gruss for helpful discussions and P. Trieu-Cuot for critical reading of the manuscript. We thank R. Gardan for the protocol of B. subtilis transformation.
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FOOTNOTES |
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* Corresponding author. Mailing address: Unité des Agents Antibactériens, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: (33) (1) 45 68 83 20. Fax: (33) (1) 45 68 83 19. E-mail: pcourval{at}pasteur.fr.
Present address: New York University Medical Center, Department of
Cell Biology, New York, NY 10016.
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REFERENCES |
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Top
Abstract
Introduction
Materials and Methods
References
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Journal of Bacteriology, June 1999, p. 3368-3374, Vol. 181, No. 11
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Copyright © 1999, American Society for Microbiology. All rights reserved.
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