INTRODUCTION

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The osmoregulatory system in Saccharomyces cerevisiae represents a particularly novel signal transduction pathway because it combines widely utilized mechanisms for cellular information processing observed in both prokaryotic and eukaryotic cells. The molecular components of this signal-response pathway have been identified previously by classic yeast genetics (reviewed in references 5, 34, and 54). External changes in osmolarity are detected at the cell surface by a transmembrane autophosphorylating histidine protein kinase, SLN1 (35). Signals are propagated in the cytoplasm by a series of steps involving phosphoryl transfer from SLN1 to YPD1 to SSK1 (Fig. 1), which all share sequence and functional similarity with bacterial "two-component" signal transduction proteins (30, 37). Phosphorylation of SSK1 acts as a molecular switch in controlling downstream effectors, which in the yeast osmosensing pathway consist of a mitogen-activated protein (MAP) kinase cascade. Ultimately, activation of the MAP kinase-dependent pathway leads to an increase in intracellular glycerol concentration (2, 3, 6, 31).

View larger version (7K): [in this window] [in a new window]   FIG. 1.   His-to-Asp phosphorelay in S. cerevisiae osmoregulation. The four-step phosphorelay system involving the SLN1, YPD1, and SSK1 proteins negatively regulates the downstream MAP kinase cascade. Under hyperosmotic stress conditions, dephosphorylation of SSK1 results in activation of the MAP kinase cascade, which raises the level of glycerol within the cell to restore osmotic balance. Solid bars indicate putative transmembrane regions.

The histidine protein kinase SLN1 is a hybrid protein in which the N-terminal region shares sequence similarity with the bacterial histidine kinase family and the C-terminal region has all the conserved features of the regulatory domain of bacterial response regulator proteins (35). SSK1 is a typical two-domain response regulator protein with the exception that the regulatory domain is located at the C terminus rather than at the N terminus (30). The SLN1 and SSK1 response regulator domains are phosphorylated on an aspartic acid residue. Phosphoryl transfer from SLN1 to SSK1 is dependent upon a histidine-phosphorylated protein intermediate, YPD1 (37). SSK1 is unique among characterized response regulator proteins in that phosphorylation acts as a negative regulator of SSK1. It is the unphosphorylated form of SSK1 that interacts with and activates the downstream MAP kinase cascade (36). Under normal environmental conditions, SSK1 must be maintained in a stably phosphorylated state; however, the mechanism(s) by which this is achieved is not yet thoroughly understood.

In recent years, it has become evident that two-component signaling strategies are utilized across several kingdoms, having recently been discovered in archaebacterial species (26, 38, 44), plants (9, 17, 19, 23, 39, 50, 52), fungal organisms (4, 7, 30, 35, 37, 43), and the slime mold Dictyostelium discoideum (42, 51, 59). Although a number of bacterial two-component signaling proteins have been investigated in kinetic detail, very little biochemical information is available for the growing list of eukaryotic two-component signaling proteins that have been discovered thus far.

Posas and coworkers (37) provided the first biochemical evidence that the eukaryotic two-component signaling proteins SLN1, YPD1, and SSK1 function in a multistep phosphorelay pathway analogous to a minor fraction of known bacterial two-component systems, notably, sporulation in Bacillus subtilis (8), virulence in Bordetella pertussis (49), and anaerobic responses in Escherichia coli (20, 21, 48). It has been postulated that the advantages of incorporating multiple phosphoryl transfer steps along a single signaling pathway are to allow for several regulatory checkpoints and possibly additional input from "cross-talk" between parallel pathways (5, 8, 37, 49).

We have initiated studies aimed at the biochemical characterization of the SLN1-YPD1-SSK1 phosphorelay pathway. In this report, part of the yeast osmoregulation system has been reconstituted in vitro by using purified domains. We have compared and contrasted the roles of the homologous regulatory domains associated with SLN1 and SSK1 by examining phosphoryl transfer from YPD1 and the lifetimes of the phosphorylated regulatory domains. Based on our findings, we propose that under normal osmolarity conditions, SSK1 is maintained in its phosphorylated inactive state, at least in part due to the unusual stability of the phosphoaspartate linkage.

	MATERIALS AND METHODS

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Materials. All chemicals and biochemicals used were of ultrapure grade. NdeI and chitin beads were obtained from New England Biolabs. Pfu DNA polymerase was purchased from Stratagene. All other restriction endonucleases, DNA-modifying enzymes, and oligonucleotides were purchased from GIBCO/BRL. H₃³²PO₄ (>9,000 Ci/mmol) was purchased from Amersham. Kodak BioMax MS film was used for autoradiography. Chromatography media were purchased from Pharmacia and Sigma. Plasmids pPD2146 and pSSK1220, containing the genes for SLN1 and SSK1, respectively, were a gift from H. Saito (Dana-Farber Cancer Institute). The expression vector encoding Salmonella typhimurium CheY, pME124, was kindly provided by A. M. Stock (University of Medicine and Dentistry of New Jersey).

Construction of plasmid expression vectors. For expression in bacterial cells, gene fragments corresponding to the regulatory domains of the yeast SLN1 and SSK1 proteins (designated R1 and R2, respectively) were amplified by PCR and subcloned into the pCYB2 vector of the IMPACT system (New England Biolabs) to generate fusion proteins consisting of the target protein located at the N terminus, followed by the yeast VMA1 protein-splicing intein domain and a chitin binding domain (CBD) at the C terminus. The SLN1-R1 and SSK1-R2 gene fragments were obtained by PCR amplification from plasmid DNA templates using Pfu DNA polymerase and synthetic oligonucleotides containing unique restriction enzyme recognition sites at the termini. Specifically, plasmid pFJS17 was constructed by subcloning an NdeI-XhoI PCR fragment containing nucleotides 3250 to 3660 of the coding region of SLN1 (encoding amino acids 1084 to 1220) into pCYB2. Similarly, another plasmid was engineered to contain nucleotides 1483 to 2136 of SSK1 (encoding amino acids 495 to 712) cloned into pCYB2 as an NdeI-SmaI fragment. Since the gene fragments were expressed as N-terminal fusion proteins, an ATG start codon was included as part of the NdeI site at the 5' end. In order to improve expression of the SSK1-R2 fusion protein, the entire coding region corresponding to the SSK1-R2-intein-CBD fusion was further subcloned and placed under the control of the T7 polymerase promoter in the pET11a expression plasmid (Novagen). This pET derivative was designated pFJS16.

Expression and purification of SLN1-R1. E. coli DH5 cells transformed with the SLN1-R1 fusion construct (pFJS17) were grown in 1 liter of Luria-Bertani medium in the presence of 100 µg of ampicillin/ml at 37°C. When the optical density (at 600 nm) of the culture reached 0.6, the cells were cooled to room temperature and expression of SLN1-R1 was induced by the addition of isopropyl--D-thiogalactopyranoside (IPTG) to a final concentration of 0.4 mM. The cultures were shaken for an additional 8 h at room temperature and then harvested, washed, and resuspended at 5 ml/g (wet weight) of cells in lysis buffer (20 mM Tris-HCl [pH 7.5], 500 mM NaCl, 1 mM EDTA, 0.1% Triton X-100). Cells were lysed by sonication, and the lysates were clarified by centrifugation at 112,000 × g for 1 h at 4°C. The supernatant was loaded onto a 1.5-ml chitin bead column equilibrated in lysis buffer at 4°C. The column was washed sequentially with 20 ml of lysis buffer, 5 ml of cleavage buffer (20 mM Tris-HCl [pH 7.5], 50 mM NaCl, 1 mM EDTA), 5 ml of cleavage buffer containing 5 mM ATP-10 mM MgCl₂, and 5 ml of cleavage buffer. The ATP-containing wash was included to remove any bound heat shock proteins (47). The column was then washed immediately with 5 ml of cleavage buffer containing 30 mM dithiothreitol (DTT), and then buffer flow was stopped. The column was incubated overnight at 4°C to allow thiol-induced cleavage of the fusion protein. The target protein was eluted with cleavage buffer. SLN1-R1 was further purified by separation on a Sephadex G50 gel filtration column (bed volume, 50 ml) equilibrated in 20 mM Tris-HCl (pH 7.5)-50 mM NaCl-1 mM EDTA-1 mM DTT. Fractions containing SLN1-R1 were pooled and concentrated by using a Centricon 10 (Amicon) filter unit. The protein was judged to be 99% homogeneous based on analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The protein concentration was determined by the absorbance at 280 nm by using a calculated extinction coefficient of 4,020 M¹ cm¹. Typical yields were 0.2 mg of pure protein/g of cells. Purified SLN1-R1 protein was stored in gel filtration buffer in the presence of 10% glycerol at 80°C.

Expression and purification of SSK1-R2. Purification of SSK1-R2 was performed similarly to that of SLN1-R1 with the following modifications. The SSK1-R2 fusion protein construct (pFJS16) was expressed in E. coli BL21(DE3) cells. When the optical density (at 600 nm) of the culture had reached 0.8, expression of the fusion protein was induced by the addition of IPTG to a final concentration of 1 mM. Changes in the buffers were as follows: the cell lysis buffer (pH 8.0) contained 20% glycerol, the cleavage buffer (pH 8.0) contained 10% glycerol, and the gel filtration buffer (pH 8.0) contained 100 mM NaCl and 10% glycerol. Final purification by gel filtration chromatography was performed by using Sephadex G75 resin. The protein was judged to be 93% homogenous based on analysis by SDS-PAGE. The protein concentration was determined by the absorbance at 280 nm by using a calculated extinction coefficient of 25,440 M¹ cm¹. Typical yields were 0.18 mg of pure protein/g of cells. Purified SSK1-R2 was stored in gel filtration buffer at 80°C.

Purification of CheY and YPD1. S. typhimurium CheY was purified from E. coli HB101 cells harboring a CheY overexpression plasmid (pME124) according to the work of Stock et al. (46). The expression and purification of yeast YPD1 have been described elsewhere (56).

Phosphorylation reactions using acetyl phosphate. [³²P]-labeled acetyl phosphate was prepared according to the method of Stadtman (45) and stored in 0.1 M Tris-HCl, pH 7.0, in small aliquots at 20°C. Unless stated otherwise, phosphorylation reaction mixtures (15 µl) contained 2 µM (each) CheY and/or YPD1 and either SLN1-R1 or SSK1-R2 in 50 mM Tris-HCl (pH 7.5)-5 mM MgCl₂-1 mM DTT. Reactions were carried out at room temperature; they were initiated by the addition of acetyl [³²P]phosphate to 2 to 5 mM and stopped by the addition of 5 µl of 4× stop buffer (0.25 M Tris-HCl [pH 6.8], 8% SDS, 40 mM EDTA, 40% glycerol, 0.008% bromophenol blue). Samples were analyzed by separation of proteins on an SDS-15% polyacrylamide gel followed by autoradiography.

Chemical stabilities of phosphorylated amino acids. Protein phosphorylation reactions containing acetyl [³²P]phosphate were performed as described above and allowed to proceed for 10 min at room temperature. Reactions were terminated by the addition of stop buffer. To assess the acid and base stability of the phospho-amino acid linkage, HCl or NaOH was added to a final concentration of 0.5 or 1 M, respectively. Reaction mixtures were incubated for 10 min at 37°C, after which the reactions were neutralized. Samples were analyzed by SDS-PAGE as described above.

Preparation of phospho-YPD1. Phospho-YPD1 was prepared by incubating YPD1 (12 µM) with CheY (3 µM) in the presence of 3 mM acetyl [³²P]phosphate in 50 mM Tris-HCl (pH 7.5)-5 mM MgCl₂-1 mM DTT in a total volume of 100 µl for 10 min at room temperature. To separate phospho-YPD1 from CheY, 20 µl of a slurry of Q Sepharose Fast Flow resin (Pharmacia) in resin buffer (50 mM Tris-HCl [pH 7.5]-5 mM MgCl₂) was added to the reaction mixture and mixed gently for 5 min. The resin was pelleted by centrifugation at 1,000 × g for 2 min and was then washed four times with 100 µl of resin buffer containing 0.1 M NaCl. Phospho-YPD1 was eluted with 60 µl of resin buffer containing 0.25 M NaCl and was used directly in phosphoryl transfer experiments with SLN1-R1 and SSK1-R2.

Phosphorylation and dephosphorylation of SLN1-R1 and SSK1-R2. Isolated radiolabeled phospho-YPD1 (0.5 µM) was incubated at room temperature with a 10- to 20-fold molar excess of SLN1-R1 or SSK1-R2 in a total volume of 100 µl. Aliquots were removed at indicated time points, mixed with 4× stop buffer to terminate the reaction, and kept at 20°C until gel analysis. Proteins were separated on an SDS-15% polyacrylamide gel and analyzed by autoradiography. Relative amounts of phosphorylated protein were determined by scanning laser densitometry of the autoradiograph. Rate constants for the dephosphorylation reaction and half-lives of the phosphorylated response regulator domains were determined by least-squares fitting of the data to a single exponential, assuming first-order kinetics.

	RESULTS

Top Abstract Introduction Materials and methods Results Discussion References

Expression and purification of the regulatory domains SLN1-R1 and SSK1-R2. The regulatory domains of the yeast osmoregulation proteins SLN1 and SSK1 were originally identified based on their conserved sequence homology to known bacterial response regulator proteins (30, 35). Each of the two domains, termed SLN1-R1 and SSK1-R2 in this report, is located at the carboxyl terminus of the respective protein. In order to investigate and compare the biochemical properties of these two domains to each other and to other bacterial response regulator proteins, we designed expression plasmids containing only the coding regions for the response regulator domains: amino acids 1084 to 1220 of SLN1 and amino acids 495 to 712 of SSK1. Both domains were overexpressed in E. coli as fusion proteins by using the IMPACT system (Fig. 2). Due to differences in expression levels and solubilities of the resulting fusion proteins, the SLN1-R1 fusion protein was expressed under the control of the P_tac promoter in DH5 cells, whereas the SSK1-R2 fusion protein was expressed under the control of the T7 RNA polymerase promoter in BL21(DE3) cells. Bacterial cultures were grown at room temperature upon induction in order to minimize the formation of inclusion bodies. After overnight incubation with DTT, which induces self-cleavage of the intein fusion protein, the isolated response regulator domains were obtained in relatively pure form (Fig. 2, lanes 4) and were further purified to near-homogeneity by gel filtration chromatography (Fig. 2, lanes 5). As expected, SLN1-R1 and SSK1-R2 migrate on an SDS-polyacrylamide gel with apparent molecular weights of 15,300 and 24,300, respectively (Fig. 2). Both domains were active in terms of phosphoryl transfer activity, as shown below.

View larger version (40K): [in this window] [in a new window]   FIG. 2.   Purification of SLN1-R1 and SSK1-R2 domains using the IMPACT fusion protein system. Protein expression and purification of SLN1-R1 (A) and SSK1-R2 (B) were analyzed by SDS-PAGE. Lanes 1, whole-cell lysates from uninduced cells; lanes 2, whole-cell lysates of induced cells, grown for 8 h at room temperature; lanes 3, soluble protein fraction after cell lysis; lanes 4, pooled fractions from chitin column following overnight thiol-induced cleavage; lanes 5, pooled fractions after gel filtration. Proteins were denatured in SDS-PAGE sample buffer in the absence of reducing agents, separated on SDS-15% polyacrylamide gels, and stained with Coomassie blue. Sideways carets (<) indicate induced expression of full-length fusion protein. Sizes of molecular markers are given on the left.

Phosphorylation of SLN1-R1 and SSK1-R2 using small-molecule phosphoryl donors. A number of bacterial response regulator domains can be phosphorylated in vitro by using small-molecule phosphoryl donors (13, 14, 28, 33, 40, 58). For example, the chemotaxis response regulator CheY can be phosphorylated by using acetyl phosphate (Fig. 3, lane 1). In order to assess the activities of the SLN1-R1 and SSK1-R2 response regulator domains, we examined the abilities of the isolated domains to catalyze phosphoryl transfer by using acetyl [³²P]phosphate. Both SLN1-R1 and SSK1-R2 were also able to use acetyl phosphate as a phosphoryl donor (Fig. 3, lanes 4 and 7), although with greatly diminished efficiency in comparison to CheY. The weak band representing phosphorylated SLN1-R1 (Fig. 3, lane 4) was observed upon longer exposure of the film.

View larger version (43K): [in this window] [in a new window]   FIG. 3.   Phosphorylation of YPD1, SLN1-R1, and SSK1-R2 by using a heterologous in vitro system. Phosphorylation reaction mixtures (15 µl) contained 2 µM (each) CheY and/or YPD1, and either SLN1-R1 or SSK1-R2 as indicated in 50 mM Tris-HCl (pH 7.5)-5 mM MgCl2-1 mM DTT. After the addition of 3 mM acetyl [32P]phosphate, reaction mixtures were incubated for 10 min at room temperature, and reactions were stopped by the addition of 5 µl of 4× stop buffer. Reaction products were separated on an SDS-15% polyacrylamide gel. Immediately following SDS-PAGE, the wet gel was wrapped in plastic film and autoradiography was performed at 80°C. Molecular size markers are indicated on the left.

Phosphorylation of YPD1 using CheY. To obtain sufficient quantities of phospho-SLN1-R1 and phospho-SSK1-R2 for biochemical studies, we developed a heterologous system that utilized the bacterial response regulator CheY. In this system, acetyl [³²P]phosphate was used to phosphorylate CheY, which in turn served as a phosphoryl donor for the yeast protein YPD1 (Fig. 3, lane 3). Phosphoryl transfer between CheY and YPD1 is reversible (data not shown). To verify proper phosphoryl transfer from the aspartic acid residue of CheY to the histidine residue of YPD1, both phosphorylated proteins were treated with 0.5 M HCl or 1 M NaOH for 10 min at 37°C. Phospho-YPD1 was stable under basic conditions but sensitive to acidic conditions, whereas phospho-CheY was sensitive to both pH extremes (Fig. 4, lanes 3 and 4). This is consistent with an acyl phosphate linkage (aspartyl-phosphate) on CheY and a phosphoramidate linkage (histidinyl-phosphate) on YPD1 (15).

View larger version (44K): [in this window] [in a new window]   FIG. 4.   Chemical stability of phosphorylated YPD1 and SSK1-R2. Phosphorylation reactions (30 µl) were carried out as described in the legend to Fig. 3 and stopped by the addition of stop buffer. The sample was divided into four aliquots, which were either left untreated (lane 1) or incubated for 10 min at 37°C alone (lane 2) or after the addition of HCl to a final concentration of 0.5 M (lane 3) or NaOH to a final concentration of 1 M (lane 4). Reaction products were separated on an SDS-15% polyacrylamide gel and analyzed by autoradiography.

Phosphoryl transfer from YPD1 to SLN1-R1 and SSK1-R2. According to the proposed phosphorelay of the yeast osmoregulation pathway, a phosphoryl group is transferred from SLN1 to YPD1, which in turn serves as a phosphoryl donor for SSK1. When either SLN1-R1 or SSK1-R2 was included in a reaction mixture containing acetyl [³²P]phosphate, CheY, and YPD1, the phosphoryl group was efficiently transferred to the yeast response regulator domain (Fig. 3, lanes 6 and 9). In the absence of CheY (data not shown) or YPD1 (Fig. 3, lanes 5 and 8), only limited direct phosphorylation of the SLN1-R1 and SSK1-R2 domains with acetyl phosphate was observed. The weak band representing phosphorylated SLN1-R1 (Fig. 3, lane 5) was observed upon longer exposure of the film.

View larger version (38K): [in this window] [in a new window]   FIG. 5.   YPD1-dependent phosphoryl transfer from phospho-CheY to SSK1-R2. CheY (3 µM) and YPD1 (12 µM) were incubated in the presence of 0.7 mM acetyl [32P]phosphate in a total volume of 100 µl in 50 mM Tris-HCl (pH 7.5)-5 mM MgCl2-1 mM DTT at room temperature. Aliquots (9 µl) were removed at the indicated time points and added to 3 µl of 4× stop buffer (lanes 1 to 4). After 30 min, SSK1-R2 was added to 4 µM, incubation was continued, and aliquots (12 µl) were removed and mixed with 4 µl of 4× stop buffer at indicated time points (lanes 5 to 9). Reaction products were separated on an SDS-15% polyacrylamide gel and analyzed as described in the legend to Fig. 3. Molecular size markers are indicated on the left.

Half-lives of the phosphorylated regulatory domains. Most phosphorylated bacterial response regulators examined to date have a relatively short half-life in the presence of magnesium ions, ranging from seconds for CheY (16) to about 10 h for the vancomycin resistance protein VanR (53). To address the stability of the phosphorylated form of SSK1-R2, and to compare it to SLN1-R1 and other response regulator proteins, we measured rates of dephosphorylation of phospho-SLN1-R1 and phospho-SSK1-R2. Purified phospho-YPD1 (0.5 µM) was incubated with a molar excess of SLN1-R1 (10 µM) or SSK1-R2 (5 µM) in the presence of MgCl₂. Phosphoryl transfer from YPD1 to SLN1-R1 or SSK1-R2 was complete within 1 min. Aliquots were removed at designated time points thereafter, and stop buffer was added. The reaction products were separated on SDS-PAGE gels and analyzed by autoradiography and densitometry (Fig. 6A and 7A). Throughout the time period analyzed, radiolabeled YPD1 was not detectable, indicating that there is no appreciable reverse reaction. The results indicate that the phosphorylated response regulator domains of SLN1 and SSK1 have distinctly different intrinsic stabilities. Phospho-SLN1-R1 exhibited a half-life (t_1/2) of 13 ± 1 min (Fig. 6) with a corresponding rate constant (k) of 0.054 min¹, whereas under identical reaction conditions, phospho-SSK1-R2 exhibited a half-life nearly 200-fold greater (t_1/2 = 42 ± 10 h; k = 0.017 h¹) (Fig. 7).

View larger version (28K): [in this window] [in a new window]   FIG. 6.   Dephosphorylation rate of phospho-SLN1-R1. (A) Autoradiograph showing dephosphorylation of phospho-SLN1-R1 as a function of time. Phospho-YPD1 (0.5 µM) was incubated with a 20-fold molar excess of SLN1-R1 in 50 mM Tris-HCl (pH 7.5)-5 mM MgCl2-1 mM DTT at room temperature for 1 min to allow complete transfer of the phosphoryl group. An aliquot was then removed, mixed with stop buffer, and labeled t0. Subsequently, aliquots were removed at specific time points over the course of 80 min and mixed with stop buffer to terminate the reaction. Proteins were separated on SDS-15% polyacrylamide gels and analyzed as indicated in the legend to Fig. 3. (B) The autoradiographs were further analyzed by scanning laser densitometry to determine the fraction of phospho-SLN1-R1 remaining. Dephosphorylation of phospho-SLN1-R1 followed first-order rate kinetics, and the rate constant and half-life of phospho-SLN1-R1 were determined accordingly. The lines represent computer-generated least-squares fitting to a single exponential or a linear relationship (inset). Data shown are from a representative experiment which was performed multiple times.

View larger version (28K): [in this window] [in a new window]   FIG. 7.   Dephosphorylation rate of phospho-SSK1-R2. (A) Autoradiograph showing dephosphorylation of phospho-SSK1-R2 as a function of time. Phospho-YPD1 (0.5 µM) was incubated with a 10-fold molar excess of SSK1-R2 as described in the Fig. 6 legend. Subsequent aliquots were removed and mixed with stop buffer over the course of 5 days. Proteins were separated on SDS-15% polyacrylamide gels and analyzed as indicated in the Fig. 3 legend. (B) Autoradiographs were further analyzed by scanning laser densitometry to determine the fraction of phospho-SSK1-R2 remaining. Dephosphorylation of phospho-SSK1-R2 followed first-order rate kinetics, and the rate constant and half-life of phospho-SSK1-R2 were determined as described in the Fig. 6 legend.

	DISCUSSION

Top Abstract Introduction Materials and methods Results Discussion References

We expressed and purified the SLN1-R1 and SSK1-R2 domains and YPD1 from E. coli in order to compare and contrast the phosphorylation and dephosphorylation activities of these two response regulator domains that are part of the yeast osmosensing pathway. Although both isolated SLN1-R1 and SSK1-R2 domains could be phosphorylated to a limited extent by using acetyl phosphate, a heterologous system consisting of acetyl phosphate, the bacterial chemotaxis protein CheY, and YPD1 was developed as a more efficient means of phosphorylating SLN1-R1 and SSK1-R2 in vitro.

Histidine-to-aspartate phosphoryl transfer. Using the heterologous in vitro phosphorylation system, we demonstrated that CheY, phosphorylated by using acetyl phosphate, can serve as a phosphoryl donor to YPD1. This interaction between the bacterial response regulator CheY and the yeast phosphorelay protein YPD1 suggests that molecular recognition elements have been conserved throughout evolution.

Stabilities of the phosphorylated response regulator domains. Studies of several response regulator domains have indicated a much shorter Mg²⁺-dependent half-life for the phosphorylated state compared to half-lives of small-molecule acyl phosphates (27), ranging from seconds for CheY and CheB (16, 55) to several hours for OmpR and Spo0F (18, 57). These domains thus exhibit an intrinsic phosphatase activity that most likely reflects the duration of the cellular response required for that particular pathway. The response regulator having the longest reported phosphorylated lifetime is VanR (t_1/2 = 10 to 12 h) (53). In comparing the in vitro stabilities of the phosphorylated response regulator domains associated with SLN1 and SSK1, we found that while SLN1-R1 exhibited a phosphorylated t_1/2 of ~13 min in the presence of Mg²⁺, in marked contrast, under identical conditions, the half-life for the phosphorylated SSK1-R2 domain was approximately 200-fold longer (t_1/2 = ~42 h), which to our knowledge is the longest half-life that has been observed for any phosphorylated response regulator domain examined to date. Our preliminary results with full-length SSK1 indicate that the phosphorylated lifetime does not differ significantly from that of the isolated R2 domain, which suggests that the N-terminal effector-like domain does not influence the lifetime of the phosphorylated protein (22).