INTRODUCTION

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The bacterial genus Chlamydia comprises four species which live as intracellular parasites in specialized vacuoles within eukaryotic cells (reviewed in reference 22). Chlamydia trachomatis is a human pathogen that infect mucous membranes in the urogenital epithelium and is the leading cause of sexually transmitted diseases. The chlamydial genome (1,043 kbp) is among the smallest of all known prokaryotic genomes (21). Such reduction of the genome size of obligate intracellular bacteria is possible because many intermediates required for their metabolism need not be synthesized by the complex pathways characteristic of free-living bacteria but are transported from the intermediate-rich host cell cytoplasm by unusual transport systems that often have no counterparts in free-living bacteria (16, 32).

Both chlamydiae (8) and Rickettsia prowazekii (30), another obligate intracellular bacterial parasite, are able to exchange their intracellular ADP for the host cell's ATP. This is a system in which the net result is the transport of energy for the benefit of the parasite. The ATP/ADP transporter in R. prowazekii (R. prowazekii translocase [RpTLC]) comprises 497 amino acids and belongs to the family of solute transporters exhibiting 12 transmembrane domains (3, 29). Recently, two functional homologues to the rickettsial ATP/ADP transporter have been identified at the molecular level in higher-plant plastids from Arabidopsis thaliana (10, 15). The amino acid sequences of the plastidic ATP/ADP transporters exhibit 62 to 66% similarity to the evolutionarily widely distant rickettsial ATP/ADP transporter. The plastidic transporters, like the rickettsial homologue, catalyze ATP import in exchange to endogenous ADP (18).

It has been demonstrated that C. psittaci exhibits an ATP/ADP exchange similar to that in R. prowazekii (8). As both bacterial species are obligate intracellular parasitic bacteria but live in very different environmental niches (rickettsiae in the eukaryotic cytosol, chlamydiae in a modified phagosome), it is of interest to identify the transporter protein mediating ATP and ADP movement across the chlamydial cell membrane. From information available from the C. trachomatis genome program, it became evident that two putative membrane proteins with substantial homology to the rickettsial ATP/ADP transporter are encoded in the genome (24).

The demonstration that both rickettsial and plastidic ATP/ADP transporters can functionally be expressed in Escherichia coli (3, 12, 15, 27) suggested that this heterologous expression system could also be used to examine the putative chlamydial transporters. In this approach, we amplified the two structural chlamydial genes via PCR, cloned and expressed the products in E. coli, and analyzed the nucleotide transport properties of the chlamydial proteins across the E. coli cytoplasmic membrane. In this study we characterized the biochemical properties of both transporters, with special attention to whether both structural genes, C. trachomatis npt1 (nucleoside phosphate transporter 1) (npt1_Ct) and C. trachomatis npt2 (npt2_Ct), encode ATP/ADP transport proteins. Answers to these questions are crucial for understanding the physiology of one of the major human pathogens.

	MATERIALS AND METHODS

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Cloning of Npt1_Ct and Npt2_Ct. Genomic C. trachomatis DNA (from serovar L2) was kindly provided by Grant McClarty (University of Manitoba, Winnipeg, Manitoba, Canada). DNA manipulations were performed essentially as described previously (20). The structural genes encoding Npt1_Ct and Npt2_Ct were amplified from the genomic DNA of C. trachomatis by PCR using Pfu DNA polymerase (Stratagene, Heidelberg, Germany), which has proofreading activity. The sense primers used, JT100 (5'-catatgactcaaaccgcggaaaaacc-3') and JT200 (5'-tagattaggaaggagcatatgtcttccgagg-3'), were constructed on the basis of data from the C. trachomatis genome program with NdeI restriction sites at the start codons of npt1_Ct and npt2_Ct, respectively. The antisense primers, JT101 (5'-ttaagaaacaccttctatagcaggagcgg-3') and JT201 (5'-ctataaagttgttacaggttcttctcgagac-3'), contained the stop codons of npt1_Ct and npt2_Ct, respectively. The PCR products obtained for npt1_Ct and npt2_Ct were gel purified and cloned into the EcoRV site of plasmid pBSK (Stratagene); the resulting plasmids were named pJT144 and pJT157, respectively. Both DNAs were sequenced on both strands by chain termination reaction (MWG-Biotech, Ebersberg, Germany). To construct E. coli plasmids expressing an N-terminal histidine tag (pJT167 [encoding His₁₀-Npt1_Ct] and pJT168 [encoding His₁₀-Npt2_Ct]), the NdeI/BamHI DNA inserts of plasmids pJT144 and pJT157 were introduced in frame into the NdeI/BamHI sites of the isopropyl--D-thiogalactopyranoside (IPTG)-inducible T7 RNA polymerase bacterial expression vector pET16b (Novagen, Heidelberg, Germany). Transformation of E. coli was carried out according to standard protocols.

Heterologous expression of ntp1_Ct and ntp2_Ct in E. coli. E. coli C43 cells transformed with plasmid pJT167 or pJT168 were grown in YT-ampicillin medium to an optical density (OD; A₆₀₀) of 0.6. Induction of T7 RNA polymerase was initiated by addition of IPTG (final concentration, 1 mM). Cells were grown to an OD of 1.3 and collected by centrifugation for 8 min at 5,000 × g (4°C). The sediments were resuspended to an OD of 1.0 in potassium phosphate (50 mM, pH 7.5) (12) and stored on ice until use within 1 h.

Uptake of radioactively labeled nucleotides. The washed E. coli cells described above (100 µl) were added to 100 µl of potassium phosphate (50 mM, pH 7.5) containing -³²P-labeled nucleotides (80 to 200 µCi/mmol). -³²P-labeled nucleoside triphosphates (NTPs) were purchased from NEN (Bad Homburg, Germany); synthesis of [-³²P]ADP from [-³²P]ATP and analysis of purity were carried out as described previously (27).

Thin-layer chromatography of radioactively labeled guanosine nucleotides. Polyethyleneamine-cellulose thin-layer chromatography was used to identify the chemical nature of the radioactivity accumulated by E. coli when incubated with GTP (13). Separation was carried out for 0.5 min with 0.5 M sodium formate (pH 3.4) and 2 min with 2 M sodium formate (pH 3.4), and the front was allowed to run for 15 cm with 4 M sodium formate (pH 3.4). R_f values of radioactively labeled nucleotides were determined after autoradiography and corresponded to R_f values of authentic standards visualized under UV light (13).

Nucleotide sequence accession numbers. The amino acid sequences of Npt1_Ct, Npt2_Ct, and RpTLC are available under EMBL database accession no. AJ010586, AJ010587, and M28816, respectively.

	RESULTS

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Sequence and hydropathy analysis of Npt1_Ct and Npt2_Ct. The genome of C. trachomatis contains two genes, named npt1_Ct and npt2_Ct, that show significant similarity at the deduced amino acid level to RpTLC. After amplification of npt1_Ct and npt2_Ct, we cloned the entire PCR products into the pBSK vector and sequenced the inserts. The deduced amino acid sequence of npt1_Ct differed from the amino acid sequence determined in the C. trachomatis genome project at position 420, where we found a Leu instead of a Met (Fig. 1). Npt1_Ct comprised 528 amino acids and exhibited 68% similarity (Genetics Computer Group PileUp program [2]) to RpTLC (Fig. 1).

View larger version (103K): [in this window] [in a new window]   FIG. 1.   Alignment of predicted amino acid sequences of Npt1Ct, Npt2Ct, and RpTLC. Numbers indicate amino acid positions; dots mark artificial sequence gaps introduced to improve similarity between the proteins.

View larger version (41K): [in this window] [in a new window]   FIG. 2.   Hydropathy analysis of the predicted amino acid sequences of Npt1Ct, Npt2Ct, and RpTLC. Hydropathy analysis was carried out as described by von Heijne (28). The values of H, which are essentially identical, have been offset to separate the plots for clarity. The predicted transmembrane domains in RpTLC are shown at the top.

Analysis of His₁₀-Npt1_Ct. We examined the transport properties of Npt1_Ct as an N-terminal His-tagged transporter fusion protein because an N-terminal histidine extension led to higher activities of the plastidic ATP/ADP transporters when expressed heterologously in E. coli (15, 27) and a histidine tag should allow purification of the fusion proteins in the future. The transport of ATP and ADP catalyzed by His₁₀-Npt1_Ct was linear for the first 2 min; after about 10 min of incubation, no substantial increase of radioactivity in the bacterial cells occurred (Fig. 3). In control experiments, uninduced E. coli cells did not import substantial radioactivity (Fig. 3; see also reference 27), and the activity of the unrelated glucose 6-phosphate and glucose transport systems did not increase after expression of His₁₀-Npt1_Ct (data not shown; see also reference 27). Increasing concentrations of exogenous [-³²P]ATP or [-³²P]ADP stimulated uptake to an apparent saturation at an external concentration of about 300 µM. A Lineweaver-Burk analysis of the data allowed us to estimate apparent K_m values of 48 µM for ATP and 39 µM for ADP and V_max values of 370 nmol of ATP · mg of protein¹ · h¹ and 625 nmol of ADP · mg of protein¹ · h¹.

View larger version (16K): [in this window] [in a new window]   FIG. 3.   Time dependency of [-32P]ATP () and [-32P]ADP () uptake into intact E. coli cells expressing npt1Ct. IPTG-induced E. coli cells harboring plasmid pJT167 were incubated with 50 µM [-32P]ATP or [-32P]ADP for the indicated time periods. Control cells were not induced (IPTG). Data are means of three independent experiments; standard deviations were less than 8% of the mean values.

View larger version (16K): [in this window] [in a new window]   FIG. 4.   Exchange-mediated efflux of intracellular radioactivity. IPTG-induced E. coli cells expressing npt1Ct were preloaded by incubation with [-32P]ATP at 25 µM. After removal of external radioactivity, cells were resuspended in phosphate buffer with or without 1 mM AMP, ADP, or ATP.

Analysis of nucleotide transport catalyzed by His₁₀-Npt2_Ct. Although E. coli cells expressing npt2_Ct were able to transport both [-³²P]ATP and [-³²P]ADP (Fig. 5A)in contrast to nucleotide transport mediated by His₁₀-Npt1_Ct (Fig. 3)transport failed to reach a rapid steady state and ADP uptake was substantially slower than ATP uptake. In addition, the substrate specificity of His₁₀-Npt2_Ct was much broader than that of His₁₀-Npt1_Ct. When GTP, UTP, and CTP were added as putative inhibitors at a concentration 2.5-fold higher than that of the substrate, [-³²P]ATP influx catalyzed by His₁₀-Npt2_Ct was reduced by 93, 80, and 69%, respectively (Table 1).

View larger version (11K): [in this window] [in a new window]   FIG. 5.   Time dependency of [-32P]ATP, [-32P]ADP, [-32P]GTP, [-32P]UTP, and [-32P]CTP uptake into intact E. coli cells expressing npt2Ct. (A) Time dependency of [-32P]ATP (0.8 mM; ) or [-32P]ADP (1 mM; ) uptake. (B) Time dependency of [-32P]GTP (0.05 mM), [-32P]UTP (0.5 mM), and [-32P]CTP (0.5 mM) uptake.

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Effect of CCCP on NTP uptake mediated by His₁₀-Npt2_Ct. Several transport systems in bacteria are energized by the symport of H⁺ driven by the proton motive force. The uptake of all four -³²P-labeled rNTPs by E. coli cells expressing npt2_Ct was markedly inhibited by the protonophore m-chlorocarbonyl cyanide phenyl/hydrazone (CCCP) (Table 1). In contrast, the exchange transport catalyzed by His₁₀-Npt1_Ct was only slightly inhibited. The uptake of [-³²P]GTP, the substrate for which His₁₀-Npt2_Ct exhibited the highest affinity, was 86% inhibited by CCCP (Table 1). Based on cell water determinations in E. coli (33), the intracellular concentration of GTP achieved at steady state in the experiment shown in Fig. 5B is approximately 1.8 mM. This represents a 36-fold (1.8/0.05) concentration gradient, of which 86% can be inhibited by CCCP. The lack of complete inhibition (in which the intracellular concentration equals the extracellular concentration) is most likely due to bound GTP and distribution of radioactivity into compounds other than NTPs. This interpretation was supported by analysis of the intracellular pool after uptake of [-³²P]GTP by thin-layer chromatography and autoradiography, which was demonstrated that about 80% of the extractable radioactivity was transportable [-³²P]GTP and 20% was nontransportable [-³²P]GDP (data not shown).

	DISCUSSION

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During the course of the C. trachomatis genome program, two putative ATP/ADP transporter proteins (herein called Npt1_Ct and Npt2_Ct) have been sequenced. In this study, we demonstrated that Npt1_Ct and Npt2_Ct catalyze nucleotide transport, but of very different sorts. There are obvious similarities in the amino acid sequences and the hydropathy profiles of Npt1_Ct, Npt2_Ct, and RpTLC. Npt1_Ct and Npt2_Ct exhibit 68 and 61% similarity to RpTLC (Fig. 1), comparable to the similarities between the two plastidic ATP/ADP transporters from A. thaliana and the rickettsial homologue (10, 15). npt1_Ct and npt2_Ct generated by PCR from genomic DNA exhibited few, but highly reproducible, differences in the deduced amino acid sequences compared to the sequences published by the C. trachomatis genome program. The differences are most likely due to the difference in serovars of C. trachomatis used for the genome analysis (serovar D) and for the experiments described here (serovar L2).

Hydropathy analysis revealed that both chlamydial proteins belong to the family of membrane-bound carriers exhibiting 12 transmembrane domains (Fig. 2), although they have no similarity in amino acid sequence to other members of this family (14). This configuration seem to represent a general feature of all nonmitochondrial nucleotide transporters analysed so far, although in a few cases 11 transmembrane domain models cannot be eliminated. The topological arrangement of the nonmitochondrial nucleotide transporters resembles those of most secondary carriers, which are constructed of 12 transmembrane domains irrespective of the substrates transported or the mode of transport (uniport, symport, or antiport) catalyzed (11). The main topological differences between the chlamydial and rickettsial transporter are significant C-terminal extensions in the chlamydial transporters, the extra amino acids in Npt2_Ct, and the elimination in Npt1_Ct of transmembrane domain 12 by the MEMSAT program (Fig. 1 and 2). Although small truncations at the C-terminal end of RpTLC resulted in drastically reduced transports rates (34) and a 10-his extension on the rickettsial C terminus did not interfere with activity (1), it remains to be established whether the C-terminal extensions of the chlamydial transporters influence their transport properties.

His₁₀-Npt1_Ct catalyzed ATP and ADP transport when expressed heterologously in E. coli (Fig. 3). The apparent affinities for the two compounds, K_ms of 48 and 39 µM, respectively, are similar to the adenylate affinities of RpTLC (K_m for ATP, 75 µM [29] or 100 µM [3]; K_m for ADP, 50 µM [18a]). Hatch et al. (8) estimated in C. psittaci cells K_m values for ATP and ADP of approximately 5 µM. The reason for this large difference is not known.

The observations that [-³²P]ATP uptake was strongly inhibited by ADP and that external ADP or ATP promoted efflux of preloaded [-³²P]ATP (Fig. 4) support the conclusions that the two compounds are transported by the same transport protein (namely, Npt1_Ct) and that both metabolites move across the cytoplasm membrane in an exchange mode of transport. These conclusions are consistent with the biochemical properties of the rickettsial ATP/ADP transporter and the plastidic homologues in A. thaliana (12, 15, 18, 27, 30), as well as with findings for C. psittaci cells (8).

The physiological functions of the plastidic and rickettsial ATP/ADP exchange systems are obvious. For example, heterotrophic plastids are unable to synthesize ATP at sufficient rates and must import ATP for anabolic reactions to proceed (4). Both C. trachomatis and R. prowazekii are obligate intracellular bacterial parasites that exploit the host cells for both nutrients and energy. While rickettsiae exhibit both oxidative phosphorylation and the ability to transport ATP, the apparent absence of both oxidative activity and an electron chain in chlamydiae supported the assumption that these bacteria are energy parasites (16) and thus require an ATP/ADP exchange to satisfy their energy needs. Information obtained in the genome project suggests that, in fact, chlamydiae may have the ability to regenerate their own ATP. Thus, most likely the exchange of host cell ATP for bacterial ADP is an alternative mechanism to acquire energy in both organisms. In C. trachomatis, this transport is obviously mediated by Npt1_Ct. After infection of eukaryotic cells with chlamydiae, the rate of glucose consumption is strongly increased and a concomitant increase of the ATP concentration occurs (19). Assuming that the inclusion membrane that surrounds the parasites is permeable to ATP, perhaps this facilitates the provision of the bacterial parasite with ATP. It is worth mentioning that little is known about transport across the inclusion membrane (6, 17).

Although Npt2_Ct exhibits remarkable structural and topological similarities to both Npt1_Ct and RpTLC (Fig. 1 and 2), Npt2_Ct possesses totally different biochemical properties. Npt2_Ct exhibited no substantial affinity for ADP, moderate affinity for ATP, UTP, and CTP, and very high affinity for GTP (Table 3). Npt2_Ct does not mediate an exchange of NTPs (as catalyzed by Npt1_Ct); rather, nucleotides accumulate in an energy-coupled manner inside the cell. For GTP, an approximately 30-fold gradient was mediated by this transporter. The inhibitory effect of CCCP on Npt2_Ct-mediated uptake of NTPs (Table 1) indicated that NTPs enter the bacterial cells in an H⁺-cotransport mode. Such a mode of transport depends ultimately on the presence of a proton motive force across the parasitic cytoplasmic membrane. Indeed, it has been shown that such a force exists in obligate intracellular bacteria (35) and supports the accumulation of lysine (8, 23). The stoichiometry between proton and NTP cotransport is unknown but must be large, since GTP is assumed to be tetravalent in the cytoplasm. If the stoichiometry is 4, then the transported complex is neutral and the driving force is pH and is independent of . Interestingly, with the high stoichiometry of 4, a pH of 0.37 is adequate to support a 30-fold gradient of tetravalent GTP. If the stoichiometry is 3, then the transported complex is negative and a pH of 1.2 would be necessary to obtain this gradient of GTP even assuming a modest of 120 mV (the situation would become worse as increased). If the stoichiometry is 5, then the complex is positively charged and no pH would be needed, and a of 89 mV would suffice as a driving force.

But what is the physiological reason for a second type of nucleotide transport system mediating net influx of molecules? GTP, ATP, UTP, and CTP are required for RNA synthesis, the formation of lipids and carbohydrate-activated intermediates, protein synthesis, and signalling. However, C. trachomatis is auxotrophic for all NTPs except CTP, which can be derived from UTP (26). Therefore, RNA synthesis, and other processes, in C. trachomatis depends strictly on the exogenous provision of the bacterium with NTPs, in agreement with data for C. psittaci cells (7, 25).

It is remarkable that transport proteins with a high degree of structural similarity exhibit a totally different mode of transport. Npt1_Ct catalyzes an exchange mode of transport with high substrate specificity for ATP and ADP, whereas Npt2_Ct exhibits high substrate specificity toward GTP and other NTPs and is energized by the proton motive force. Keeping these differences in mind, how great do the molecular differences between two proteins have to be to allow significant changes in substrate specificity or mode of catalysis? There are examples indicating that comparable small changes in the molecular architecture suffice to alter such fundamental biochemical properties. For example, the exchange of only two amino acids in the strict NAD-specific leucine dehydrogenase from Thermoactinomyces intermedius leads to an enzyme which strongly prefers NADP as a coenzyme (5). Moreover, the chemical modification of four cysteine residues in the mitochondrial ADP/ATP carrier by application of thiol reagents alters the mode of transport from obligate exchange to uniport without loss of the maximal catalytic activity (2a). It will be interesting to study the structure-function relationships in more detail by generating site-directed mutants and chimeric proteins of Npt1_Ct and Npt2_Ct.