INTRODUCTION

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Ralstonia (Pseudomonas) solanacearum, which causes a lethal wilting disease of solanaceous and many other types of other plants (15, 16), enters hosts via natural openings or wounds in roots and then proceeds to extensively colonize xylem vessels of the vascular system (37, 44). Although secreted plant cell wall-degrading exoenzymes enhance virulence (possibly by facilitating invasion and vascular colonization [23, 24, 37]), it is exopolysaccharide I (EPS I), a large, nitrogen-rich, acidic exopolysaccharide (34), that is the primary virulence factor of R. solanacearum. EPS I is produced in copious amounts and is required for wilting and killing of hosts (8, 29). EPS I apparently causes wilting by restricting water flow through xylem vessels (6). It also markedly enhances the speed and extent of stem colonization (37).

In R. solanacearum, production of EPS I (as well as some exoenzymes) is stringently controlled by a cascading network of more than 10 regulatory genes (5, 11, 20, 41). Inactivation of any of seven genes in this network causes a >85% reduction in transcription from the eps promoter (P_eps), leading to loss of EPS I production and the ability to wilt and kill. However, inactivation of all but two of these genes (vsrB and vsrC) can be suppressed by constitutive expression of xpsR from a vector promoter (20). These and other data showed that VsrB, VsrC, and XpsR are the most downstream components in the eps regulatory cascade and suggested that they may directly affect interaction of RNA polymerase with P_eps.

The predicted amino acid sequences of VsrB and VsrC imply that they comprise a two-component system in which VsrB is a sensor kinase and VsrC is its cognate response regulator (19, 20). However, no homologs of the very basic XpsR protein have been found. How these three proteins interact to control P_eps is not clear. Nonetheless, analogy to other two-component systems (17) implies that, in response to some unknown signal, VsrB phosphorylates VsrC, thereby stimulating it to turn on transcription, possibly via direct binding to P_eps. EpsR, a putative DNA-binding protein, is another potential regulator of P_eps, since its overproduction strongly represses EPS I synthesis by R. solanacearum strain K60 (18, 25).

Since there was no clear or direct evidence for physical interactions between P_eps and VsrC, XpsR, or EpsR, we first used deletion and PCR mutagenesis to define an upstream region of P_eps that is absolutely required for its transcription activation by VsrB-VsrC and XpsR. Then we generated a vsrC allele that activated P_eps independently of vsrB and used DNase I footprinting to show that both this constitutively active VsrC protein and wild-type VsrC directly bind to the region of P_eps that was identified by mutagenesis to be important in transcription activation. However, since the affinity of VsrC for P_eps was weak and since the constitutively active VsrC protein was unresponsive to VsrB and still required XpsR for activation of P_eps, we speculate that XpsR may facilitate or stabilize binding of VsrC to P_eps.

	MATERIALS AND METHODS

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Bacteria and plasmids. Most strains and plasmids constructed and used in this work are described in Table 1. R. solanacearum was grown in BG or BSM medium (36) at 30°C, while Escherichia coli was grown in LB (33) at 37°C. The host strain and vectors used for cloning were E. coli DH5 (14) and pTZ19U/18U (31), respectively. pPF12 (7) and pEPS1 (21) were described previously. R. solanacearum strain AW22 was constructed by transposon mutagenesis with Tn3lacZ (7). Derivatives of AW22 were constructed by natural transformation (20) with genomic DNA from AW-R164, AW-C, and AW-MG2 (19). Concentrations (micrograms per milliliter) of antibiotics used to select and maintain plasmids were kanamycin (Km), 50; spectinomycin (Sp), 50; ampicillin (Amp), 100; and tetracycline (Tc), 20. 

Construction of P_eps reporter plasmids. P_eps fragments with various lengths of upstream sequences were made by PCR using primers EPS1 (5'-CCGGATCCCCAACTGTAAATCGTA-3'), EPS2 (5'-CCGGATCCAAACGAAATATGCATT-3'), EPS3 (5'-CCGGATCCTTTCGGTATTGAAGC-3'), EPS4 (5'-CCGGATCCATGCACAACCGTATC-3'), EPS5 (5'-TCGGATCCATCGCCACCGGTACTG-3'), EPS6 (CCGGATCCAGAACGATCCATGTTTC-3'), EPS7 (5'-CCCCGGGTTGGCGTTCTGCCTAT-3'), EPS9 (5'-GAGGATCCAGTTGCAGAAACGGCCA-3'), M13F (TGTAAAACGACGGCCAGT-3'), M13R (5'-AGCGAATAACAATTTCACACAGGA-3'), and T7 (5'-TAATACGACTCACTATAGGG-3'). PCR mixtures (100 µl) contained 10 mM Tris (pH 8.3), 50 mM KCl, 1 mM of each deoxynucleoside triphosphate (dNTP), 2 mM MgCl₂, 0.1 nmol of primers, 1 to 5 ng of template, and 2 U of Taq polymerase (Perkin-Elmer). Each amplification cycle consisted of denaturation at 94°C for 1 min, annealing at 50°C for 1 min, and extension at 72°C for 1 min. After 30 cycles, there was a final extension at 72°C for 10 min. The P_eps DNA fragments from nucleotides 538 to +23, 337 to +23, and 243 to +23 were amplified using pXPS1 as template and primer pairs EPS6-EPS4, EPS7-EPS4, and EPS9-EPS4, respectively. The P_eps DNA fragments from 143 to +23, 101 to +23, 68 to +23, and 44 to +23 were amplified using primer pairs M13F-EPS4, EPS1-EPS4, EPS2-EPS4, and EPS3-EPS4, respectively, and pXPS5 as template. PCR products were digested with BamHI and/or SmaI and ligated into the promoterless lacZ fusion vector pRG970 (43) digested in the same manner. After transformation into E. coli and plating on LB agar with Sp and 5-bromo-4-chloro-3-indolyl--D-galactopyranoside (X-Gal), plasmid DNA was isolated from blue colonies, and the orientation and identity of the insert were confirmed.

PCR mutagenesis of P_eps and vsrC. To mutagenize P_eps, sequences between nucleotides 143 and +23 were PCR amplified essentially as above using a pTZSZ12 template. However, the dATP concentration was 0.2 mM instead of 1 mM, and MnCl₂ was added to 0.5 mM. BamHI-digested PCR products were ligated with BamHI-digested pRG970 and transformed into E. coli, and ~10,000 blue-colored transformants arising on LB plates with Sp and X-Gal were individually picked and pooled, and plasmid DNA was isolated from them. Pooled plasmids were electroporated into R. solanacearum strain AW201 (epsA1::nptl). After plating on BG agar with Sp and X-Gal, white or pale-blue transformants (i.e., those with decreased LacZ activity) were observed at 0.3%. Plasmids from these colonies were individually isolated, transformed into E. coli, reisolated, and analyzed with restriction enzymes to confirm P_eps inserts. Sequence alterations were determined after recloning inserts into pTZ19U.

Purification of His-tagged VsrC proteins. Wild-type vsrC was PCR amplified using pKVC3 as template and primers M13F and VSRCN (5'-ACGGATCCACGAGCTCGCTGCG-3'; complementary to sequences encoding the N terminus of VsrC). The product was digested with BamHI and cloned in frame to the hexahistidine-encoding tag of BamHI-digested pTrc-HisA (Invitrogen), yielding plasmid pVSRCH. To generate pCB92, the SacI-KpnI fragment of pVSRCH was replaced with the analogous fragment from pVSRC1.

Construction of an epsR null mutant. The epsR homolog of R. solanacearum strain AW was PCR amplified from genomic DNA as above except that annealing and extension were at 70°C. Primers (EPSRN, 5'-AAGGATCCAGG CGGCGCAGTG-3' [nucleotides 355 through 381 with an added BamHI site] and EPSRC, 5'-ATGAATTCAGCCCGGCGTGCACGAGGCG-3' [nucleotides 1,055 through 1,075 with an added EcoRI site]) were based on the sequence of epsR from R. solanacearum strain K60 (GenBank M61197). The resultant PCR product was digested with BamHI and EcoRI and cloned into pTZ18U. DNA sequencing showed that epsR from strain AW is >97% identical to epsR from strain K60.

Measurement of PglA. Assay samples were spotted onto nitrocellulose and after blocking for 2 h at 25°C with 5% skim milk (Difco) in TBS (10 mM Tris HCl [pH 7.4], 140 mM NaCl), the membrane was washed three times with TBST (TBS with 0.2% Tween 20) and submerged for 1 h in 10 ml of TBST containing a 1:1,000 dilution of anti-PglA antiserum (39). After three washes with TBST, one with TBST containing 0.85 M NaCl, and one with TBST, bound PglA antibodies were detected with anti-rabbit immunoglobulin G (IgG) conjugated to alkaline phosphatase (Jackson Immunologicals), 5-bromo-4-chloro-3-indolyl-phosphate, and nitroblue tetrazolium (32).

DNase I footprinting. Target DNA fragments were prepared by PCR essentially as described above and previously (46) using 0.2 mM dNTPs, pPSZ17 as template, and primers *M13L (5'-[6-FAM]-CACGACGTTGTAAAACGACGGCCAGT-3'; PE Applied Biosystems) and T7. The resultant PCR product (containing sequences between 337 and +23 of P_eps and labeled at one 5' end with the 6-FAM fluorescent tag) was gel purified, and 40 ng (10 nM) was used in 10-µl footprinting-reaction mixtures that contained 10 mM Tris-HCl (pH 7.5), 5 mM KCl, 1 mM EDTA, 8% glycerol, and 0.5 to 4 µm (0.4 to 3.5 µg) of purified His-tagged VsrC proteins. The total protein concentration was maintained at 4.5 mg/ml using bovine serum albumin. After 30 min at 30°C, reaction mixtures were placed at 26°C, and 5 µl of RNase-free DNase I (1.2 × 10⁵ U/µl, freshly diluted [Boehringer Mannheim]) were added. After 4 min, 15 µl of 0.5 M EDTA (pH 8.0) was added; reactions were extracted with phenol-chloroform and passed through a CENTRI-SEP column (Princeton Separations). Digestion products were vacuum dried, dissolved in 12 µl of deionized formamide, 0.2 µl of GS-500-ROX size standards (PE Biosystems) was added, and the fragmentation patterns were analyzed with an ABI 310 Genetic Analyzer as described previously (46).

General molecular and genetic techniques. Methods for plasmid isolation from E. coli or R. solanacearum and subsequent transfer into E. coli using the CaCl₂-treated competent cells or into R. solanacearum via electroporation were described earlier (21, 22). Restriction enzymes, DNA ligase, Klenow fragment, and other DNA enzymes were used according to the manufacturer's recommendations. DNA sequences were obtained using an ABI 377 sequencer. Other general molecular genetic techniques used are described elsewhere (1, 28).

	RESULTS

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Two regions of the eps promoter are involved in its transcriptional activation. To define the extent of upstream sequences that are required for transcriptional activation of P_eps, we constructed a series of reporter plasmids with various lengths of P_eps fused to lacZ. Expression of these reporter genes was assayed in an R. solanacearum wild-type strain and in strains lacking key regulators. A reporter plasmid with sequences between 538 and +23 of P_eps fused to lacZ(pPSZ15) gave a high level of P_eps expression that was strongly dependent on both xpsR and vsrC, since P_eps expression was reduced at least 50-fold by inactivation of either regulator (Fig. 1). Deletion of sequences upstream of 337 or 243 did not dramatically alter expression or regulation, indicating that sequences between 243 and +23 are sufficient for wild-type expression from P_eps. When sequences between 243 and 143 were deleted (pPSZ12 [Fig. 1]), expression was reduced about threefold, suggesting that a site in the region between nucleotides 243 and 143 stimulates P_eps expression. Residual expression from this reporter construct, however, remained strongly dependent on both vsrC and xpsR. Deletion of sequences between 143 and 101 did not significantly reduce transcription below that observed with a reporter having the 143 to +23 region of P_eps fused to lacZ. However, when sequences between nucleotides 101 and 68 were deleted, P_eps expression was reduced to less than 5% of the levels observed with the 243 to +23 fusion. Expression was only marginally further reduced when P_eps sequences down to 44 were deleted (pPSZ22 [Fig. 1]), regardless of the genetic background. These data show that the 101 to 68 region contains sequences that are absolutely required for activation of P_eps by these two regulators.

View larger version (18K): [in this window] [in a new window]   FIG. 1.   Identification of upstream regions involved in transcriptional regulation of Peps by VsrC and XpsR. The eps promoter and various lengths of upstream sequences were fused to lacZ on pRG970, generating plasmids pPSZ12 through pPSZ22. Plasmids were transferred into R. solanacearum wild-type (WT) strain AW, into strain AW-C, a vsrC mutant, and into strain AW-R164, an xpsR mutant. Peps expression (i.e., transcription directed by the Peps fragment) was monitored by measuring LacZ activity (given in Miller units [33]) in cells from cultures grown overnight in BG medium as described previously (4, 20). LacZ activity from cells harboring an empty pRG970 vector was 15. Nucleotide numbering is relative to the transcription start site of eps (21). The hatched boxes indicate the two important regions for activation of eps. The sequence at the top is that of the region identified by PCR mutagenesis as essential for transcription activation by xpsR and vsrC. +, positions of substitution mutations that reduced or eliminated activation of Peps (see Table 2). Underlined sequences, putative palindromic recognition sequences for VsrC. Values are averages from three experiments with <25% variation.

Determination of nucleotides required for activation and regulation of P_eps. To delineate more precisely the sequences that are critical for P_eps regulation, a fragment with P_eps nucleotides 143 to +23 was subjected to mutagenic PCR, and the products were joined to lacZ on pRG970 to generate transcriptional fusions. The resultant pooled reporter constructs were introduced into wild-type R. solanacearum, and colonies were screened for altered P_eps::lacZ expression by using X-Gal. Sixteen mutant plasmids were obtained and characterized further by DNA sequencing and quantitative LacZ assays (Table 2). P_eps::lacZ expression from plasmids with single-nucleotide substitutions at P_eps nucleotides 74, 71, 70, and 69 was reduced ~10-fold, almost to the basal levels given by plasmids in which all upstream activation sequences had been deleted (compare to pPSZ22 and pPSZ21 [Fig. 1]). When assayed in xpsR or vsrC mutants of R. solanacearum, expression directed by these mutant promoters was only marginally reduced, indicating that these mutations largely eliminate activation of P_eps transcription by VsrC and XpsR. Fusion plasmids with single nucleotide substitutions at P_eps nucleotides 82, 79, 72, 68, and 67 were reduced about fivefold. However, when assayed in xpsR or vsrC mutant backgrounds of R. solanacearum, P_eps expression from these promoters was reduced an additional three- to fourfold to basal levels, indicating that these mutant promoters are inefficiently activated by VsrC and XpsR. In summary, these results suggest that nucleotides between 82 and 62, and in particular the sequence GTGGGGAA between 74 and 67 (Fig. 1), are important for activation of P_eps. It is plausible that this region contains the binding sites for P_eps activators, perhaps VsrC and/or XpsR.

EpsR can inhibit transcription activation of P_eps by XpsR and VsrC. epsR encodes a putative DNA-binding protein that inhibits EPS production by R. solanacearum strain K60, but only when plasmid borne (18, 25, 30). Moreover, the two reported phenotypes of epsR mutants of strain K60 are contradictory (3, 25). Therefore, we needed to explore a possible role for epsR in regulating P_eps transcription in our R. solanacearum strain. To do this we transferred plasmid pKL4 containing the K60 epsR gene (25) into our wild-type AW strain harboring a genomic eps::lacZ fusion (AW19A). The addition of pKL4 reduced expression of eps by sevenfold (Table 4). To determine what P_eps sequences are required for this effect, pKL4 was transferred into strain AW harboring reporter plasmids with different lengths of P_eps sequences fused to lacZ (Fig. 1). Expression from reporters with P_eps sequences between 143 and +23 or between 101 and +23 fused to lacZ was specifically reduced by greater than ninefold by the presence of pKL4 (Table 4). However, deletion of P_eps sequences between nucleotides 101 and 44 eliminated this effect. When pKL4 was placed in a strain harboring fusion plasmid pEPSM9 or pEPSM3, which cannot be transcriptionally activated due to mutations in the 82 to 62 regulatory region, expression from P_eps was not significantly decreased. Thus, epsR affects P_eps only if it has been activated, suggesting that epsR interferes with transcriptional activation of P_eps mediated by vsrC and xpsR via the 82 to 62 upstream region.

Isolation and characterization of vsrB-independent vsrC alleles. Previous genetic studies (19, 20) and analogy to other two-component systems (17) suggest that, in response to some signal, VsrB phosphorylates VsrC, converting it into a form that can activate transcription from P_eps. However, since XpsR is also required, this assumption is tentative. To explore the dependency of VsrC on the VsrB sensor kinase, as well as XpsR, we set out to isolate vsrC alleles that activate P_eps independently of VsrB and/or XpsR. A pool of 20,000 plasmids containing heavily PCR-mutagenized vsrC alleles was transferred into R. solanacearum strain AW22B (eps::lacZ vsrB::), and transformants with elevated eps::lacZ expression were selected. After reisolation and retransformation into AW22B, only one plasmid (pVSRC1) consistently and strongly increased P_eps expression in the absence of VsrB. LacZ assays showed that in either AW22B or AW22BC (vsrB vsrC eps::lacZ) double mutants, pVSRC1 caused a greater than ninefold increase in transcription of eps::lacZ (Table 5). In contrast, pRKVC3 with wild-type vsrC only slightly increased eps expression. This suggested that the vsrC1 allele on pVSRC1 harbors a mutation that makes it nearly fully active in the absence of phosphorylation by VsrB. When pVSRC1 was placed in the wild-type reporter strain AW22 or strain AW22C (vsrC eps::lacZ), P_eps activation was similar to that observed in strain AW22BC (Table 5), suggesting that the activity of VsrC1 cannot be dramatically increased by VsrB. When pVSRC1 was placed in a strain lacking VsrC, VsrB, and XpsR (AW22RBC), transcription of eps::lacZ showed only a small (less than twofold) increase. Restoring vsrB to this strain (i.e., converting it to a vsrC xpsR mutant) had no effect on its vsrC1-mediated activation of P_eps (data not shown). Thus, although VsrC1 protein is very active without VsrB, it still requires xpsR for P_eps activation. Not surprisingly, all of our attempts to isolate vsrC alleles that functioned independently of xpsR by screening the pool of mutant vsrC alleles in xpsR mutants were unsuccessful.

View larger version (73K): [in this window] [in a new window]   FIG. 2.   Regulation of pglA by wild-type vsrC and mutant alleles of vsrC. Concentrated supernatants of 24-h-old BSM cultures of strain AW22 (wild-type background [WT]) or from double mutant strain AW22BC (vsrB vsrC) containing the indicated plasmids were serially diluted twofold in BSM and 5 µl of each dilution (2× through 1/8×) spotted on nitrocellulose. The amount of PglA was assayed using anti-PglA antiserum and alkaline phosphatase-conjugated secondary antibody. pRKVC3 contains wild-type vsrC; pVSRC1 and pVSRC2 contain the vsrB-independent alleles vsrC1 and vsrC2, respectively. PglA, supernatant from R. solanacearum PG3 (pglA::nptl [39]). Purified PglA (50, 25, 12, 6, and 3 µg, respectively, for each of the 5 dilutions listed at the left) was used for calibration.

In vitro analysis of VsrC binding to P_eps. Genetic data could not distinguish whether VsrC directly binds to and activates P_eps or works via an intermediate, so we investigated the ability of VsrC to bind to P_eps in vitro. First we constructed expression plasmids harboring either wild-type vsrC or the constitutively active vsrC1 with their N termini translationally fused in frame to a hexahistidine encoding tag. Both His-tagged alleles were nearly as active as wild-type vsrC; when cloned on pRK415 and placed in an R. solanacearum vsrC mutant, both alleles increased eps::lacZ expression by >10-fold, to ca. 50% of wild-type levels (data not shown). After both His-tagged proteins were purified to >90% homogeneity, up to 20 µg of each was used in gel mobility shift assays with appropriate ³²P-labeled P_eps fragments. However, no consistent specific mobility shifting was detected under a variety of conditions (data not shown).

View larger version (53K): [in this window] [in a new window]   FIG. 3.   DNase I footprinting analysis of VsrC binding to Peps. Reactions were set up, processed, and analyzed using the ABI 310 as described in Materials and Methods and previously (46). (A) Electropherograms from reactions with increasing amounts of His-VsrC. The y-axis gives fluorescence intensity, which is proportional to fragment abundance; the x-axis gives elution position of fragments, which is proportional to their size. Bottom scale gives nucleotide position relative to the eps transcription start site (21) determined using the elution positions of internal size standards. Solid bar, upstream region of Peps that is protected from DNase I digestion by His-VsrC. Dashed line, region made hypersensitive by VsrC. The DNA sequence of the protected region, marked with the same bars, is shown above with nucleotides identified by mutagenesis as critical for transcription activation (see Table 2) marked +. (B) "False gel image" representation of electropherograms in panel A generated by Genotyper 2.5 software, which converts the fluorescence intensity of peaks into proportional gray-scale bands. The sequence of the VsrC-protected region is given on the right.

	DISCUSSION

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Transcription of eps, an operon encoding biosynthesis of the EPS I virulence factor of R. solanacearum, is controlled by a complex network composed of at least three interacting and environmentally responsive systems (41). We previously found that activation of transcription driven by P_eps sequences downstream of nucleotide 143 was completely dependent on xpsR and the vsrB-vsrC two-component system (21). Extending that work here, we showed that sequences downstream of nucleotide 243 are required and sufficient for wild-type P_eps expression and regulation. However, when P_eps sequences between 243 and 143 were deleted, expression was reduced threefold but remained fully dependent on xpsR and vsrB-vsrC. Thus, the 243 to 143 region enhances the transcription activation that is mediated by these regulators. The global virulence regulator of R. solanacearum, PhcA (5, 41), may play a role in this enhancement, because purified PhcA binds to and protects the 185 to 140 region of P_eps from DNase I digestion and because the enhanced activation that requires the 243 to 143 region is absent in phcA mutants (our unpublished data).

Further promoter deletion experiments clearly showed that nucleotides below 101 are absolutely critical for activation of P_eps. Subsequent mutagenesis studies (Table 2) revealed that many of the critical nucleotides lie between 82 and 62, in particular within the GTGGGGAA located between 74 and 67. Inactivation of either vsrC or xpsR did not further reduce expression from P_eps fragments with mutations in this region, suggesting that VsrC and/or XpsR may directly bind to this site to mediate transcription activation. Using DNase I footprinting we confirmed that VsrC does indeed directly bind to and protect the 74 to 67 region. Recent footprinting experiments (W. Yindeeyoungyeon and M. Schell, unpublished data) have identified another VsrC-protected binding site upstream of a new eps gene. The sequences of these two VsrC-protected sites show extensive similarity and suggest that VsrC may recognize a conserved palindromic consensus sequence found in both regions (TCCNC-N₈-GGGGA; Fig. 1). However, in contrast to similar footprinting experiments with another DNA-binding protein (46), a >100-fold excess of wild-type or constitutively active VsrC did not fully protect either site from DNase I, implying that the affinity of VsrC for these sites is relatively low. Perhaps XpsR (or another factor) is required for strong binding. In support of this hypothesis, we found that transcriptional activation by both wild-type and the constitutively active vsrC1 allele still requires xpsR. Although enhancement of DNA binding by an auxiliary protein is not a common property of response regulators (17), another R. solanacearum response regulator, VsrD, also requires an auxiliary protein for its transcriptional regulation (22). Unfortunately, in vitro testing for the effect of XpsR on VsrC binding has not been possible due to the insolubility of our purified XpsR preparation. Alternatively, it is plausible that XpsR may be involved in the phosphorylation status of VsrC; however, the activity of VsrC1 in vivo and in vitro was essentially wild type regardless of the presence of the VsrB sensor kinase. While these data suggest that VsrC1 is fully active in the absence of phosphorylation, other possibilities remain. Site-directed mutagenesis of Asp-58 in VsrC's receiver domain, the presumed site of phosphorylation by VsrB, should better define the role of VsrB and XpsR in phosphorylation and/or activation of VsrC .

The position of the substitution in VsrC that conferred independence from VsrB (residue 209, 12 residues from the C terminus) is interesting and novel, but not surprising considering that vsrC1 was the only bona fide VsrB-independent allele found in a population of >20,000 heavily mutagenized alleles. VsrC, a FixJ-type response regulator (17), is very similar to NarL, the crystal structure of which has been determined (2). Alignment of the C termini of VsrC and NarL (which are 80% similar) suggests that the S209L substitution is located in the middle of helix 10 which follows the helix-turn-helix DNA-binding domain. The region containing helix 10 has not been implicated in transcription activation by response regulators, nor is its amino acid sequence highly conserved. However, circumstantial evidence that the C terminus of a response regulator may interact with RNA polymerase has been reported: deletion of the last two or three residues of BvgA severely inhibited growth of Bordetella pertussis, but this effect was suppressed by mutations affecting the -subunit of RNA polymerase (42). We have found that vsrC1 also can cause growth inhibition in R. solanacearum. Since genetic and biochemical evidence suggests that interactions between transcriptional regulators and the -subunit of RNA polymerase are sometimes important in transcriptional activation (35), it is plausible that the C-terminal region of some response regulators (e.g., helix 10) may interact with RNA polymerase to stimulate transcription. Site-directed mutagenesis and chemical cross-linking studies are required to further investigate this possibility.

In contrast, most mutations conferring sensor independence on response regulators are N terminal (e.g., V88L for NarL [10] and some N-terminal deletions [13]). These mutations are thought to mimic changes caused by sensor kinase-mediated phosphorylation; i.e., they cause a conformational change that alleviates occlusion of the C-terminal helix-turn-helix DNA-binding domain (9, 12). Similarly, the substitution at the C terminus of VsrC1 may also alter its conformation in a way that relieves or prevents inhibitory interactions of its helix-turn-helix DNA-binding domain with other parts of the polypeptide that block or reduce its function.

The role of EpsR in P_eps regulation and its relationship to xpsR and vsrC remain unclear, because we found that inactivation of epsR did not dramatically affect expression from P_eps or EPS I biosynthesis, whereas when present on a multicopy plasmid, epsR did reduce P_eps expression by greater than sevenfold. This inhibitory effect occurred only when the VsrC-binding site at P_eps was intact. Although indirect evidence for the binding of EpsR to P_eps has been reported (3), we were unable to confirm this. Preliminary analyses with lacZ reporters show that elevated levels of EpsR slightly (threefold) reduce expression of xpsR but not of vsrC. Although this implies that environmentally directed overproduction of EpsR could reduce levels of XpsR and hence shut down EPS production, further in vivo and in vitro studies are needed to clarify the physiological role and mechanism of action of EpsR and XpsR with VsrC at the 82 to 62 region of P_eps.