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Journal of Bacteriology, February 2000, p. 1181-1184, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Two Extracytoplasmic Function Sigma Subunits, ^E and ^FecI, of Escherichia coli: Promoter Selectivity and Intracellular Levels

Hiroto Maeda, Miki Jishage, Tasuku Nomura, Nobuyuki Fujita, and Akira Ishihama^*

National Institute of Genetics, Department of Molecular Genetics, Mishima, Shizuoka 411-5840, Japan

Received 30 August 1999/Accepted 17 November 1999

	ABSTRACT

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The promoter selectivity of two extracytoplasmic function (ECF) subfamily  subunits, ^E (²⁴) and ^FecI (¹⁸), of Escherichia coli RNA polymerase was analyzed by using an in vitro transcription system and various promoters. The E^E holoenzyme recognized only the known cognate promoters, rpoEP2, rpoHP3, and degP, and the E^FecI recognized only one known cognate promoter, fecA. The strict promoter recognition properties of ^E and ^FecI are similar to those of other minor  subunits. Transcription by E^E and E^FecI was enhanced by high concentrations of glutamate, as in the case of other minor  subunits. The optimum temperature for transcription by E^FecI was low, around 25°C, apparently in agreement with the high rate of iron sequestration by E. coli at low temperatures. By quantitative Western blot analysis, the intracellular levels of ^E and ^FecI in the uninduced steady-state culture of E. coli W3110 (type A) were determined to be 0.7 to 2.0 and 0.1 to 0.2 fmol per µg of total proteins (or 3 to 9 and 0.4 to 0.9 molecules per cell), respectively, and less than 1% of the level of the major ⁷⁰ subunit.

	TEXT

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The DNA-dependent RNA polymerase of Escherichia coli is composed of the core enzyme with the subunit structure ₂' and one of seven molecular species of the  subunit, ⁷⁰, ^N, ^S, ^H, ^F, ^E, or ^FecI (11, 13). Molecular properties and functional specificity have been studied in detail for all of these  subunits except for two, ^E and ^FecI. The ^E subunit encoded by the rpoE gene controls transcription of the genes for extracytoplasmic stress response (3-5, 8, 25, 26, 31). The synthesis of ^E is induced upon exposure to heat shock or ethanol stress or following accumulation of unfolded proteins in the periplasm (8, 26, 29, 33). The holoenzyme E^E is responsible for transcription of at least 10 genes (32), of which 4 have been identified, including rpoH, which encodes ^H for transcription of the heat shock response genes (9, 31); degP, which encodes a periplasmic protease for degradation of misfolded proteins (14, 23, 31, 34); fkpA, which encodes a periplasmic peptidyl-prolyl isomerase (4); and rpoE itself (31). On the other hand, the fecI gene was originally identified as a regulatory gene for the ferric dicitrate transport system (30), but after sequencing, the FecI protein was recognized as a member of the extracytoplasmic function (ECF) subfamily of  factor (hereafter referred to as ^FecI in this report) (1, 24). Transcription of the ferric dicitrate transport system of E. coli is repressed by Fe²⁺-Fur and activated by ferric dicitrate (2, 7, 12). Ferric dicitrate does not have to enter into the cytoplasm for transcription activation, but it initiates a signal transduction pathway by binding to the outer membrane receptor FecA (2, 12). The signal is then transmitted through the inner membrane-associated FecR, which ultimately activates the ^FecI subunit. The fecA promoter is the only one identified to date that is transcribed specifically by the E^FecI holoenzyme.

Here we performed the first systematic analysis of the promoter of ^E and ^FecI by using in vitro transcription assay systems. In addition, we determined the intracellular concentrations of these two ECF subfamily  subunits in E. coli W3110 (A) growing at various phases.

Promoter selectivity of the E^E and E^FecI holoenzymes. For analysis of the promoter selectivity of RNA polymerase holoenzymes containing ^E or ^FecI, two ECF family  subunits were overexpressed in E. coli M15 by using plasmid pRPOE (14) or E. coli BL21(DE3) by using plasmid pETFecI. The plasmid pETFecI for the expression of ^FecI protein with a hexahistidine tag (His₆) at the C terminus was constructed by insertion of PCR-amplified FecI-coding sequence into pET-21b (Novagen) between the NdeI and HindIII sites. Both ^E and ^FecI were extracted from the inclusion bodies with an extraction buffer containing 0.5% Triton X-100 and purified by Ni²⁺-nitrilotriacetic acid affinity chromatography. For reconstitution of the holoenzymes, we purified the -free core enzyme by chromatography of the purified RNA polymerase of E. coli W3350 (10) at least three times through phosphocellulose columns (6, 22). The repeated chromatography is essential for complete removal of traces of minor  subunits. To detect the activity in vitro of purified ^E, we used truncated DNA templates, each containing one of the three known promoters, i.e., the 210-bp EcoRI-SphI rpoE promoter fragment, the 220-bp EcoRI-SphI rpoH fragment, or the 214-bp EcoRI-SphI degP fragment, each producing specific transcripts of 71 (rpoE), 81 (rpoH), and 74 (degP) nucleotides in length, respectively. For detection of the ^FecI activity, we used the only known FecI-dependent promoter, fecA, which produces RNA of 62 (fecA) nucleotides in length.

Under the standard transcription assay conditions for the E⁷⁰ holoenzyme (E represents the core enzyme) (20), the reconstituted holoenzymes containing ^E and ^FecI produced specific transcripts directed by the respective cognate promoters (Fig. 1). None of the other holoenzymes containing ⁷⁰, ^N, ^S, ^H, or ^F, however, produced significant levels of transcripts from the ^E-dependent rpoE, rpoH, and degP promoters or from the ^FecI-dependent fecA promoter, even though all of these holoenzymes gave similar levels of the template-sized end-to-end transcripts, which migrated near the top of the gels (Fig. 1). On the other hand, both E^E and E^FecI holoenzymes were unable to transcribe the ⁷⁰-dependent lacUV5 (Fig. 1), trp, and rpsA promoters. Thus, we concluded that the ECF family  subunits carry high selectivity for a specific set of the cognate promoters, as in the case of other minor  subunits, ^N, ^H, and ^F. In contrast to the strict promoter selectivity characteristic of the minor  subunits, the ⁷⁰ subunit recognizes in vitro most ^S-dependent promoters, and the ^S subunit recognizes some ⁷⁰-dependent promoters (21, 35).

View larger version (114K): [in this window] [in a new window]   FIG. 1.   Transcription in vitro of truncated DNA templates by RNA polymerase holoenzymes EE and EFecI. RNA polymerase holoenzymes were reconstituted by mixing the core enzyme with each  subunit in a core-to- molar ratio of 1:4. Single-round transcription was carried out under the standard assay conditions (20) with 1 pmol each of seven different holoenzymes containing 70, N, S, H, H, F, H, and FecI (the species of  subunit used is shown at the bottom of each gel lane) and 0.1 pmol each of three E-dependent (rpoE [A], rpoH [B], and degP [C]) and one FecI-dependent (fecA [D]) promoter. RNA products were separated by 8% PAGE in the presence of 8 M urea, and gels were analyzed with a Bio-Imaging Analyzer BAS-2000 (Fuji). Arrowheads indicate the specific transcripts from the test promoters, while open triangles indicate the template-sized nonspecific transcripts.

Stimulation of ^E- and ^FecI-dependent transcription by potassium glutamate. The reaction conditions such as DNA superhelicity, the species and concentrations of salts, trehalose, and polyphosphate, and the reaction temperature affect in vitro transcription in different ways for the different holoenzymes containing different  subunits, presumably reflecting the difference in physiological conditions under which each  subunit works (reviewed in references 15 and 16).

The standard reaction mixture to give maximum-level transcription of lacUV5 by the E⁷⁰ holoenzyme contains 50 mM NaCl (Fig. 2C) (see also reference 20). The optimum concentrations of NaCl to give maximum transcription activity on trp, recA, and rpsA templates were between 50 and 100 nM (data not shown). The activity of E⁷⁰ holoenzyme is, however, negligible at NaCl concentrations above 200 mM (Fig. 2C) (see also references 8 and 28). In contrast, transcription of rpoE by E^E (Fig. 2A) and of fecA by E^FecI (Fig. 2B) stays almost at the same level, between 50 and 300 mM NaCl, indicating that transcription by these two holoenzymes is relatively resistant to inhibition by high NaCl concentrations. Previously, we found that high concentrations of glutamate enhance transcription by the E^S and E^F holoenzymes (6, 22). Here we also examined the effect of increasing concentrations of potassium glutamate. As shown in Fig. 2A and B, transcription by both E^E and E^FecI holoenzymes was significantly enhanced upon increasing the potassium glutamate concentration up to at least 400 mM. The molecular mechanism underlying the activation of minor -dependent transcription by high concentrations of glutamate remains to be solved.

View larger version (16K): [in this window] [in a new window]   FIG. 2.   Effects of salt concentrations on in vitro transcription by RNA polymerase EH and EFecI holoenzymes. Single-round transcription was carried out by using 0.1 pmol of each of three test promoters, rpoE (A), fecA (B), and lacUV5 (C), and 1 pmol each of three different forms of the reconstituted holoenzyme, EE (A), EFecI (B), and E70 (C), under the standard reaction conditions except that 50 mM NaCl was replaced by the indicated concentrations of either NaCl or K glutamate. Transcripts were fractionated by 8% PAGE, and gels were examined with a Bio-Imaging Analyzer BAS2000 (Fuji). The maximum levels of transcription observed with each template are set at 100%.

Preference for low temperatures of ^FecI-dependent transcription. The optimum temperature for maximum-level transcription by the regular holoenzyme E⁷⁰ is about 37°C, whereas the optimum temperature of transcription of certain promoters by E^H and E^F significantly deviates from this optimum temperature for the E⁷⁰ holoenzyme (22, 36). We then examined the effect of reaction temperature on rpoE promoter-directed transcription by E^E and fecA promoter-directed transcription by E^FecI. As shown in Fig. 3A, the optimum temperature for maximum transcription by E^E was 37°C, but about half the maximum-level activity was retained above 50°C, indicating that transcription by E^E predominates at high temperatures, in good agreement with the expected role of ^E in response to extremely high temperatures (8).

View larger version (13K): [in this window] [in a new window]   FIG. 3.   Effect of temperature on in vitro transcription by RNA polymerase EE and EFecI holoenzymes. Single-round transcription was carried out with 0.1 pmol each of two test promoters, rpoE (A) and fecA (B), and 1 pmol each of either EE (A) or EFecI (B) holoenzyme under the standard reaction conditions in the presence of 400 mM K glutamate. Both preincubation for RNA polymerase-promoter complex formation and incubation for transcription were carried out at the various temperatures indicated. The maximum levels of transcription observed with each template are set at 100%.

In contrast, fecA promoter-directed transcription by E^FecI was at the maximum at around 25°C and linearly decreased thereafter up to 52°C, where the activity was almost negligible (Fig. 3B). Among seven species of the holoenzyme examined under the same conditions, the E^FecI species required the lowest temperature to give the maximum activity of transcription. The acquisition of iron by bacteria is known to be more efficient at low temperatures (37). The in vivo activation of ^FecI-dependent transcription at low temperatures awaits further analysis.

Intracellular levels of the ^E and ^FecI proteins. Previously, we determined the intracellular concentrations of ⁷⁰, ^N, ^S, ^H, and ^F in E. coli W3110 (type A lineage) by the quantitative Western blot method (17, 19). Here we determined the concentration of two ECF family subunits, ^E and ^FecI, in the same cell extracts of E. coli W3110 (A) used in our previous determination. In the exponential growth phase, the concentrations of ^E and ^FecI were 0.7 to 2.0 and 0.1 to 0.2 fmol per µg of total proteins, respectively (Fig. 4). In the same extract, the concentration of the major  subunit, ⁷⁰, is 150 to 170 fmol/µg of total proteins (17). The number of ⁷⁰ molecules per cell is estimated to be around 700 (19). The numbers of ^E and ^FecI molecules per cell can then be calculated to be 3 to 9 and 0.4 to 0.9, respectively. The level of ^E stayed constant throughout the growth phase examined, but the ^FecI level further decreased in the stationary phase (Fig. 4). The critical factors leading to induction of the synthesis or activation of the ECF family  subunits, however, remain unclear. Taken together with the previous determinations (17, 19), we conclude that under the steady state of cell growth, the uninduced levels of the minor subunits ^S, ^H, ^E, and ^FecI are all lower than 1% of the level of the major ⁷⁰ subunit.

View larger version (32K): [in this window] [in a new window]   FIG. 4.   Intracellular concentrations of E and FecI in E. coli W3110 (type A). E. coli W3110 (type A) was grown with shaking in Luria-Bertani medium at 37°C. Cell extract was prepared by the method of Jishage et al. (17, 19). The protein concentration of cell lysates was determined by using the Bio-Rad protein assay kit. Polyclonal antibodies against E and FecI were raised in rabbits by injecting the overexpressed and purified  proteins. The quantitative Western blot analysis was employed for the measurement of  subunits exactly as described in previous reports (17, 19). The immunostained blots were developed with 3,3'-diaminobenzidine tetrahydrochloride (Dojindo). Staining intensity was measured with a PDI image analyzer system equipped with a white light scanner. (A) Aliquots containing 10 µg of total proteins from cell lysates of E. coli W3110 (A) prepared at various times of the cell culture (see B for the growth curve) were subjected to quantitative Western blot analysis using anti-E and anti-FecI antibodies. (B) E. coli W3100 (type A) was grown in Luria-Bertani medium at 37°C under the same conditions employed in the determination of other  subunits (17, 19), and growth was monitored by measuring the turbidity with a Klett-Summerson photometer. At the indicated time points labeled 1 to 7, aliquots were taken for preparation of the cell lysates.

In addition to the synthesis control, the activity is negatively regulated, at least in the case of ^E subunit, by a membrane-bound anti- factor, RseA (5, 27). Such an activity control of the  subunit has been found for both ^F (28) and ⁷⁰ (18). However, the factor affecting the ^FecI activity has not yet been identified.

We thank S. Kusano and T. S. Kundu for preparation of ^S, ^H, and ^F and A. Iwata and S. Ueda for preparation of anti-^E and anti-^FecI antibodies.

This work was supported by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan and from CREST (Core Research for Evolutional Science and Technology) of the Japan Science and Technology Corporation.

	FOOTNOTES

^* Corresponding author. Mailing address: Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan. Phone: 81-559-81-6741. Fax: 81-559-81-6746. E-mail: aishiham{at}lab.nig.ac.jp.

Permanent address: Kagoshima University, Faculty of Fisheries, Kagoshima 890-0056, Japan.

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Journal of Bacteriology, February 2000, p. 1181-1184, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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