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Journal of Bacteriology, February 2000, p. 898-904, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Function of a Principal Na+/H+ Antiporter, ShaA, Is Required for Initiation of Sporulation in Bacillus subtilis

Saori Kosono,1,* Yoshiaki Ohashi,2,dagger Fujio Kawamura,2 Makio Kitada,1 and Toshiaki Kudo1

Microbiology Laboratory, Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-0198,1 and Laboratory of Molecular Genetics, College of Science, Rikkyo (St. Paul's) University, Toshima-ku, Tokyo 171-8501,2 Japan

Received 19 August 1999/Accepted 30 November 1999


    ABSTRACT
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

ShaA (sodium/hydrogen antiporter, previously termed YufT [or NtrA]), which is responsible for Na+/H+ antiporter activity, is considered to be the major Na+ excretion system in Bacillus subtilis. We found that a shaA-disrupted mutant of B. subtilis shows impaired sporulation but normal vegetative growth when the external Na+ concentration was increased in a low range. In the shaA mutant, sigma H-dependent expression of spo0A (PS) and spoVG at an early stage of sporulation was sensitive to external NaCl. The level of sigma H protein was reduced by the addition of NaCl, while the expression of spo0H, which encodes sigma H, was little affected, indicating that posttranscriptional control of sigma H rather than spo0H transcription is affected by the addition of NaCl in the shaA mutant. Since this mutant is considered to have a diminished ability to maintain a low internal Na+ concentration, an increased level of internal Na+ may affect posttranscriptional control of sigma H. Bypassing the phosphorelay by introducing the sof-1 mutation into this mutant did not restore spo0A (PS) expression, suggesting that disruption of shaA affects sigma H accumulation, but does not interfere with the phosphorylation and phosphotransfer reactions of the phosphorelay. These results suggest that ShaA plays a significant role at an early stage of sporulation and not only during vegetative growth. Our findings raise the possibility that fine control of cytoplasmic ion levels, including control of the internal Na+ concentration, may be important for the progression of the sporulation process.


    INTRODUCTION
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

All living cells actively extrude sodium ions and maintain an inwardly directed gradient of sodium concentration (33). Sodium extrusion is important as a detoxification process, because internal sodium inhibits many metabolic activities when present at high concentrations (29, 41). The major Na+-extruding mechanism in most bacterial cells is the Na+/H+ antiporter, which extrudes Na+ in exchange for H+ (32, 42). This process is driven by an electrochemical gradient of proton across the cytoplasmic membrane, which is established by the respiratory chain or the H+-translocating ATPase (55). Besides its role in Na+ extrusion, the Na+/H+ antiporter plays important roles in pH homeostasis (3, 32), cell volume regulation (17), and establishment of an electrochemical potential of Na+ (33).

Most of the Na+/H+ antiporters reported to date are encoded by single genes (6, 26, 27, 39, 44, 49, 52, 53). However, recent reports have demonstrated the existence of a novel type of cation/H+ antiporters usually encoded by a cluster of seven genes (20, 25, 45). Hiramatsu et al. (20) have recently reported the features of the mnh (multisubunit Na+/H+ antiporter) locus encoding an Na+/H+ antiporter in Staphylococcus aureus. All seven genes (mnhA to mnhG) are required for the antiporter activity, suggesting that the Mnh antiporter consists of seven kinds of subunits and forms a huge ion transport complex. Homologues of the mnh locus have been found in alkaliphilic Bacillus sp. strain C-125, Rhizobium meliloti, and Bacillus subtilis. These may be considered to be members of a multisubunit antiporter family. This gene family was first discovered in alkaliphilic Bacillus sp. strain C-125 (18). The homologue first identified in Bacillus sp. strain C-125 is related to the Na+/H+ antiporter and is required for pH homeostasis in an alkaline environment (18). The pha (pH adaptation) locus of R. meliloti contains a whole set of the seven corresponding genes and is required for invasion of nodule tissue to establish nitrogen-fixing symbiosis (45). pha mutants show sensitivity to K+, but not to Na+, in their growth and are deficient in diethanolamine-induced K+ efflux (45). It seems that the pha locus in R. meliloti may encode a K+/H+ antiporter which is involved in pH adaptation during the infection process (45).

A whole set of the seven genes has also been found in the gram-positive endospore-forming B. subtilis (25). We have recently shown that disruption of the first gene, yufT, results in a decrease in Na+/H+ antiporter activity and impaired growth when the external sodium concentration is increased, indicating that yufT encodes a Na+/H+ antiporter which has a dominant role in the extrusion of cytotoxic sodium (31). Ito et al. have more recently shown that the same set of seven genes is transcribed as an operon and that the operon is responsible for cholate resistance and pH homeostasis as well as sodium resistance (25).

Endospore formation in B. subtilis has been extensively studied at the molecular level as a simple model system with which to understand cellular differentiation. Only a few studies have focused on the role of ions or ion transport in sporulation of B. subtilis. Mn2+ and Fe2+ are known to be essential for sporulation (5, 13), and transport of Mn2+ and Ca2+ is activated during sporulation (46). It has been shown as well that the activities of several proteins related to sporulation, including KinA (16), SpoIIE (11), and RapB (51), are dependent on divalent cations. However, little is known about the role of monovalent cations in sporulation. In the present study, we found that disruption of yufT leads to a diminished Na+ excretion capacity (31), which entails sporulation defects, when the external sodium concentration is increased. To identify the stage at which the function of the Na+/H+ antiporter is required, we examined the expression of early sporulation genes in the yufT mutant, and our findings suggest the possibility that intracellular Na+ levels may play a role in posttranscriptional regulation of sigma H, an alternative sigma factor required for an initial process in the course of sporulation in B. subtilis. This report is the first to provide evidence of a relationship between Na+ and sporulation in B. subtilis.

We have previously proposed renaming yufT as ntrA (Na+ transporter) (31). However, to avoid confusion with ntr of nitrogen regulation, we have again renamed the operon by including yufT as sha (sodium/hydrogen antiporter).


    MATERIALS AND METHODS
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

Bacterial strains and culture conditions. The B. subtilis strains used in this study are listed in Table 1. All strains were derived from UOT1285, used as the wild-type strain in our laboratory. The shaA::neo allele contains a neomycin-resistant cassette inserted into the EcoRV site in shaA as described previously (31).

                              
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  		TABLE 1.   B. subtilis strains used in this study

B. subtilis cells were routinely cultivated in LB1/2 (10 g of Difco tryptone, 5 g of Difco yeast extract, and 5 g of NaCl per liter [pH 7.0]) in the case of shaA+ strains or LBK1/2 (10 g of Difco tryptone, 5 g of Difco yeast extract, and 5 g of KCl per liter [pH 7]) in the case of shaA mutant strains. Neomycin (7.5 µg/ml) and chloramphenicol (5 µg/ml) were added for selection when necessary. The nutrient sporulation medium used was modified 2× SG medium [16 g of Difco nutrient broth per liter, 1 g of KCl per liter, 1 mM MgSO4, 1 mM Ca(NO3)2, 1 µM FeSO4, 10 µM MnCl2, 1 g of glucose per liter] (34). S7K minimal sporulation medium supplemented with 0.1% (wt/vol) glucose is identical to S7 medium (14), except that sodium glutamate was replaced with potassium glutamate. Cultures were incubated at 37°C with vigorous shaking. Growth was monitored by measuring the optical density at 660 nm (OD660).

Transformation of B. subtilis. Competent B. subtilis cells were prepared and transformed by the method previously described (10). When isolating shaA::neo strains, cells were grown in modified CI (CIK) medium [14 g of K2HPO4 per liter, 6 g of KH2PO4 per liter, 2 g of (NH4)2SO4 per liter, 1 g of tripotassium citrate dihydrate per liter, 5 mM MgSO4, 5 g of glucose per liter, 50 µg of L-tryptophan per ml, 50 µg of L-lysine per ml, 1 g of yeast extract per liter] until early stationary phase. A 0.5-ml portion of the culture was centrifuged, and the cell pellet was resuspended in 1 ml of modified CII (CIIK) medium [14 g of K2HPO4 per liter, 6 g of KH2PO4 per liter, 2 g of (NH4)2SO4 per liter, 1 g of tripotassium citrate dihydrate per liter, 5 mM MgSO4, 5 g of glucose per liter, 25 µg of L-tryptophan per ml, 25 µg of L-lysine per ml, 0.5 g of yeast extract per liter]. A 0.1-ml portion of the suspension of competent cells was mixed with DNA and incubated at 37°C for 90 min with shaking. Then, 0.3 ml of CIK medium was added, and the mixture was further incubated for 60 min. An appropriate volume of the culture was then spread on a CIK plate containing 7.5 µg of neomycin per ml.

Assay of spore formation. Cells were grown in 2× SG medium, and spores were assayed 22 h after the end of the exponential phase (T22). The number of viable cells per ml of culture was determined as the total number of CFU on LB1/2 (shaA+ strains) or LBK1/2 (shaA mutant strains) plates. The number of spores per ml of culture was determined as the number of CFU after heat treatment (80°C, 10 min).

Assay of beta -galactosidase activity. The expression of transcriptional bgaB fusions was monitored by measuring thermostable beta -galactosidase activity (21, 22). Cells were grown in 2× SG medium, and 50- to 500-µl samples of the cultures were removed at appropriate times for the assay. The cell pellets were toluenized in 0.5 ml of Z buffer (60 mM Na2HPO4, 40 mM NaH2PO4, 10 mM KCl, 1 mM MgSO4, 50 mM 2-mercaptoethanol). After preincubation at 62°C, reactions were started by adding 0.2 ml of 4 mg of o-nitrophenyl-beta -D-galactoside (ONPG) per ml to the samples and stopped by adding 0.5 ml of 1 M Na2CO3. After centrifugation, the A420 of the supernatants was measured. The specific activity was expressed as 1,000 × (A420 per min of incubation per ml of culture per OD660 unit).

Western blot analysis. Western blot analysis was performed as described by Asai et al. (2). Cells were grown in 2× SG medium, and the same amount of cells (the culture volume × OD660 = 5) was collected in each instance at appropriate times. The cell pellets were resuspended in 50 µl of lysis buffer A (50 mM Tris-HCl, 1 mM EDTA, 50 g of glycerol per liter, 0.1 M NaCl, 0.1 mM dithiothreitol, 2 mM phenylmethylsulfonyl fluoride, 2 mg of lysozyme per ml [pH 7]) and incubated at 37°C for 6 min to allow cell lysis to occur. Then, 1.5 µl of lysis buffer B (330 mM MgCl2, 6.6 mg of DNase I per ml, 16.6 mg of RNase A per ml) was added, and the mixture was further incubated for 6 min. Aliquots of the whole-cell extracts (15 µg of total protein) were diluted in sodium dodecyl sulfate (SDS) sample buffer, boiled for 5 min, and subjected to electrophoresis on an SDS-12% polyacrylamide gel. Thereafter, the proteins were electrophoretically transferred to a polyvinylidene difluoride membrane (Bio-Rad) by using a Mini Trans-Blot transfer cell (Bio-Rad) in a semidry condition. The blotted membrane was incubated with primary anti-sigma H antibody (2) and secondary goat anti-rabbit immunoglobulin G alkaline phosphatase conjugate (Sigma) and finally reacted with 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitroblue tetrazolium salt (both supplied by Boehringer Mannheim) to detect signals.


    RESULTS
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

Sporulation of the shaA mutant is impaired with an increase in external sodium concentration. As shown previously, the shaA-disrupted strain shows diminished Na+/H+ antiport activity and impaired growth when the external NaCl concentration was increased, and it is therefore considered to have a diminished Na+ excretion capacity (31). A 2× SG sporulation medium containing 0.1% glucose was found to have a pH of 6.2 to 6.4, and it contained 18 mM Na+ as a contaminant, as determined by atomic absorption analysis. Under these conditions, SK6, a shaA-disrupted derivative of UOT1285, grew exponentially at a rate almost equal to that of the wild type, as shown in Fig. 1. This finding was consistent with results reported previously (31). As shown in Table 2, the numbers of viable cells at T22 were the same when comparing SK6 and the wild type (both ~108 cells/ml). The number of spores formed at T22, however, was somewhat lower in the case of SK6 (~107 spores/ml) than that of the wild type (~108 spores/ml), as shown in Table 2. When 30 mM NaCl was added to the medium, sporulation of SK6 was severely affected, resulting in less than 10 spores per ml (Table 2), whereas vegetative growth of SK6 was little affected (Fig. 1). On the other hand, addition of NaCl at concentrations up to 200 mM affected neither vegetative growth nor sporulation of the wild type (Table 2) (some data not shown).


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  		FIG. 1.   Growth in 2× SG medium containing 0.1% glucose. Strains UOT1285 (shaA+ [circles]) and SK6 (shaA::neo [triangles]) were grown at 37°C in the absence (open symbols) and presence (solid symbols) of 30 mM NaCl. Growth was monitored by measuring the OD660.

                              
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  		TABLE 2.   Sporulation of the shaA mutant and the wild type

We considered that 18 mM Na+ present as a contaminant in the 2× SG medium may have weakly inhibited the sporulation of SK6. In order to eliminate the effect of the contaminating Na+, the sporulation of SK6 was also examined in S7K medium, which contained less than 1 mM Na+ as a contaminant. SK6 produced 108 spores per ml, the same level as that of the wild type, in S7K medium without added NaCl. The sporulation of SK6 was clearly impaired when the external NaCl concentration was increased. When 50 mM NaCl was added to S7K medium, the SK6 cells reached stationary phase at a lower cell density (~107 cells per ml versus ~108 cells per ml without NaCl), and produced 105 spores per ml, 1,000-fold less than that in the absence of added NaCl. Moreover, the addition of 100 mM NaCl severely blocked the sporulation of SK6 (Table 2). The effect of the added NaCl on SK6 sporulation was apparently due to the increase in Na+ concentration, rather than being a nonspecific effect of increased ionic strength and/or increased osmolarity, because 50 mM KCl had no effect on sporulation (Table 2) and 25 mM Na2SO4 reduced the spore titer to ~105 per ml, the same level as that observed in the presence of 50 mM NaCl (data not shown). The effect of NaCl on sporulation of SK6 was more severe in 2× SG medium than in S7K medium. Both 2× SG medium with 30 mM NaCl added and S7K medium with 50 mM NaCl contain ~50 mM Na+; however, SK6 produced less than 10 spores in the former and ~105 spores in the latter.

The primary environmental signal for initiation of sporulation is nutrient depletion (12). The severe effects on sporulation are often linked to a decrease in growth rate or growth yield. The vegetative growth rate of SK6 was little affected by the addition of 30 mM NaCl (Fig. 1), but we cannot exclude the possibility that the cells do not fully metabolize components of the medium that may act as inhibitors of sporulation. To exclude this possibility, we examined whether the sporulation of SK6 is inhibited by NaCl when added after the end of exponential growth (T0). SK6 cells were grown in 2× SG medium without added NaCl, and NaCl was added at a final concentration of 30 mM at the times indicated in Fig. 2. The sporulation of SK6 was severely inhibited by NaCl when added just at T0 or before T3 (3 h after the end of exponential growth), but was not affected when added at T3 and after. These results clearly indicate that the addition of 30 mM NaCl specifically affects sporulation events in SK6 which precede T3, but not vegetative growth.


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  		FIG. 2.   Effect of NaCl on sporulation of a shaA::neo strain. SK6 (shaA::neo) cells were grown in 2× SG medium containing 0.1% glucose without added NaCl, and 30 mM NaCl was added to the medium at the indicated times after the end of exponential growth (T0). Relative frequency of sporulation is the spore production per milliliter relative to that of SK6 when grown in 2× SG without added NaCl (4.8 × 106 spores/ml).

In 2× SG medium with 30 mM NaCl added, freshly isolated SK6 produced less than 10 spores, but the strain produced 104 to 106 spores under the same conditions after the cells had been repeatedly cultured on LBK1/2 (including 7.5 µg of neomycin per ml) plates. There is a possibility that additional mutations occurred in the repeatedly cultured SK6 cells. Thus, we always used cells freshly prepared from a stock culture of this strain in the following experiments.

Induction of spo0A (PS) expression at the onset of sporulation is blocked by external NaCl in the shaA mutant. Whether or not B. subtilis cells initiate sporulation is believed to be decided by the intracellular level of the phosphorylated form of Spo0A (Spo0A-P), which is the key transcription factor that regulates early sporulation genes positively or abrB negatively (23). Phosphorylation of Spo0A occurs through a multicomponent phosphorelay that involves three sensor kinases (KinA, KinB, and KinC), a response regulator (Spo0F), and a phosphotransferase (Spo0B) (4). Transcription of spo0A is regulated by two promoters, PV and PS, which are, respectively, recognized by E-sigma A during vegetative growth and by E-sigma H at an early stage of sporulation (7). Transcription of spo0A from the PS promoter also requires its own product, Spo0A-P (47). Since sporulation of SK6 was impaired when the external Na+ concentration was increased, it seemed likely that one or more steps in the sporulation process may become sensitive to Na+. We first examined the effect of the disruption of shaA on the expression of spo0A from PS at the time of the onset of sporulation.

As shown in Fig. 3A, transcription of spo0A from PS was induced in the wild type 1 h after T0 in the presence or absence of 30 mM NaCl. On the other hand, in the absence of 30 mM NaCl, the rate of spo0A (PS) induction was lower in the shaA mutant than that of the wild type. Moreover, spo0A (PS) induction was almost completely blocked in the shaA-disrupted mutant by the addition of 30 mM NaCl. The expression of spo0A (PS) in the shaA mutant was also blocked by 30 mM NaCl when added just at T0, the same as when the mutant was grown in 2× SG with 30 mM NaCl (data not shown). It is, therefore, most likely that the shaA mutation blocks spore development by affecting the formation of Spo0A-P and/or the active sigma H-containing RNA polymerase at an early stage of sporulation.


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  		FIG. 3.   Expression of sigma H-dependent genes in shaA+ (RIK10 and RIK51) and shaA::neo (SK610 and SK651) strains using the bgaB gene coding for a heat-stable beta -galactosidase as a reporter. Cells of shaA+ (circles) and shaA::neo (triangles) strains carrying a spo0A (PS)-bgaB (A) or spoVG-bgaB (B) fusion were grown at 37°C in 2× SG medium containing 0.1% glucose in the absence (open symbols) and presence (solid symbols) of 30 mM NaCl. Samples were taken at the indicated time to determine the extent of growth and to measure beta -galactosidase activity as described in Materials and Methods.

The effect of the sof-1 mutation on the shaA mutant. Spo0A-P is generated through an active phosphorelay system, and it induces the transcription of the components of the system, spo0F as well as spo0A itself, from their sigma H-dependent PS promoters at the time of the onset of sporulation (47, 48). This allows the stimulation of the phosphorelay in a positive feedback manner and then an increase in the level of Spo0A-P at an early stage of sporulation (48). Since the addition of 30 mM NaCl severely blocked the induction of spo0A (PS) expression in the shaA mutant, it may be that the phosphorelay becomes stagnant under these conditions because of an insufficient supply of Spo0A protein. If disruption of shaA affects Spo0A-P production by inhibiting the phosphorelay, it is expected that spo0A (PS)-bgaB expression would be restored when Spo0A is phosphorylated independent of the phosphorelay. We therefore introduced the sof-1 mutation (24, 28), which is a mutation within the spo0A locus and which serves to bypass the need for spo0F in the phosphorylation of Spo0A, into the shaA mutant and examined whether spo0A (PS) induction was restored or not in the presence or absence of 30 mM NaCl.

As shown in Fig. 4, the rate of spo0A (PS) induction in the shaA sof-1 double mutant was lower than that in the wild type in the absence of added NaCl, and the induction was blocked by the addition of 30 mM NaCl. Moreover, sporulation of the shaA sof-1 double mutant occurred with the same yield of spores produced as that in the case of the shaA mutant (~105 to 107 spores per ml in 2× SG medium and less than 10 spores per ml in 2× SG medium with 30 mM NaCl at T22).


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  		FIG. 4.   Effect of the sof-1 mutation on spo0A (PS) transcription in a shaA::neo strain. Cells carrying a spo0A (PS)-bgaB fusion were grown at 37°C in 2× SG medium containing 0.1% glucose in the absence (open symbols) and presence (solid symbols) of 30 mM NaCl. Circles, RIK62 (sof-1 spo0FDelta S shaA+); triangles, SK662 (sof-1 spo0FDelta S shaA::neo).

A posttranscriptional regulation of sigma H is affected in the shaA mutant. The finding that the sof-1 mutation failed to restore spo0A (PS) induction in the shaA mutant suggested that the defect in spo0A (PS) expression in the shaA mutant may involve the loss of sigma H function. We therefore examined the expression of another sigma H-dependent gene, spoVG, in the shaA mutant. As shown in Fig. 3B, induction of spoVG expression was severely inhibited by the addition of 30 mM NaCl, as in the case of spo0A (PS) expression. These results clearly showed that disruption of shaA resulted in Na+ sensitivity of sigma H-dependent transcription during sporulation.

It is expected that the defects in sigma H-dependent transcription are the result of a decrease in the intracellular level of sigma H protein and/or a decrease in E-sigma H transcription activity. We therefore assayed the level of sigma H protein in extracts of cells grown in 2× SG medium in the presence or absence of 30 mM NaCl by Western blot analysis with anti-sigma H antibody. As shown in Fig. 5, the level of sigma H in the wild-type cells began to increase at T0, reached a maximum at T2, and then rapidly decreased after T3, which was in good agreement with results reported previously (38, 40). On the other hand, the level of sigma H in the shaA mutant increased from T0 through T2 and began to decrease at T3 in the absence of added NaCl. The sigma H level at T1 and T2 was slightly lower than that of the wild type, which may explain the lower rate of induction of the sigma H-dependent genes. In the presence of 30 mM NaCl, sigma H accumulated at T0, but the level of sigma H decreased after T1 in the shaA mutant. The addition of NaCl did not affect sigma H accumulation in the wild type. Thus, we concluded that the defects in sigma H-dependent transcription in the shaA mutant are the result of impaired accumulation of sigma H protein at an early stage of sporulation.


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  		FIG. 5.   Western blot analysis of sigma H protein from whole-cell extracts of UOT1285 (shaA+) and SK6 (shaA::neo) cells. Cells were grown at 37°C in 2× SG medium containing 0.1% glucose with or without 30 mM NaCl and harvested at the time indicated. Aliquots of cell lysates (15 µg of total protein) were electrophoresed and analyzed as described in Materials and Methods.

It has been shown that the intracellular level of sigma H protein increases at the onset of sporulation through both transcriptional and posttranscriptional mechanisms, and then the sigma H-dependent genes are induced (2, 19, 54). Since the addition of NaCl inhibited the accumulation of sigma H in the shaA mutant, we next determined the level of expression of spo0H, which encodes sigma H. Its promoter is recognized by E-sigma A and is repressed by AbrB during the vegetative phase (54). As shown in Fig. 6, in the absence of added NaCl, the induction of spo0H in the shaA mutant showed normal timing and the level of expression of spo0H was somewhat higher than that in the wild type. In the presence of 30 mM NaCl, the spo0H expression level in the shaA mutant was lower than that in the absence of NaCl, but it was not significantly lower compared with that in the wild type. In particular, the induction rates from T0 through T2 were almost the same for the shaA mutant and the wild type. These results indicated that the decrease in the sigma H level in the shaA mutant that occurred with the addition of NaCl was not due to a decrease in spo0H transcription. These results suggested that posttranscriptional regulation of sigma H was mainly affected in the shaA mutant by the addition of 30 mM NaCl.


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  		FIG. 6.   Expression of a spo0H-bgaB transcriptional fusion in shaA+ (RIK50 [circles]) and shaA::neo (SK650 [triangles]) strains. Cells were grown at 37°C in 2× SG medium containing 0.1% glucose in the absence (open symbols) and presence (solid symbols) of 30 mM NaCl.


    DISCUSSION
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

We showed here that disruption of the Na+/H+ antiporter, ShaA, results in a Na+-sensitive sporulation-deficient phenotype. It is not the growth defect in the shaA mutant that causes the sporulation defect. Since ShaA is the major Na+ extrusion mechanism in B. subtilis (31), the shaA-disrupted mutant is considered to have a diminished ability to maintain a low internal sodium concentration. The defect in sporulation resulting from the disruption of shaA is, therefore, considered to be due to inhibition of some steps in the sporulation process by an increased level of internal Na+. Furthermore, such steps sensitive to Na+ seem to be limited at early stages (before T3).

To understand which step or steps in sporulation are affected by Na+, we examined the expression of early sporulation genes in the shaA mutant. We found that the sigma H-dependent expression of both spo0A (PS) and spoVG was severely affected in the shaA mutant by the addition of 30 mM NaCl. At the same time, the level of sigma H protein was diminished in the mutant cells. The accumulation of sigma H protein was already observed at T0 in SK6 cells, in the presence or absence of 30 mM NaCl. Asai et al. (2) found a similar effect on sigma H accumulation when glucose and glutamine were in excess. Introduction of the sof-1 mutation into the shaA mutant to bypass the phosphorelay did not restore spo0A (Ps) expression, suggesting that the primary defect in sporulation conferred by the shaA::neo mutation is not directly related to phosphorylation of Spo0A.

It is likely that sigma A-dependent transcription is not affected by the shaA mutation, as shown by the absence of any defect in the vegetative growth. The fact that spo0H transcription, which depends on sigma A, was not affected in the shaA mutant shows that the shaA mutation affects some steps in sigma H control subsequent to spo0H transcription. The expression of spo0H is repressed by the repressor AbrB during the vegetative phase of growth and is induced at the onset of sporulation by repression of abrB transcription by Spo0A-P (54). Since less Spo0A-P is needed for the repression of abrB than for the activation of spo0A (PS) or stage II genes (8, 37, 50), it is likely that a small amount of Spo0A-P, but enough to allow spo0H expression through the AbrB pathway, would be produced in the shaA mutant.

The existence of a posttranscriptional regulatory mechanism(s) governing the level of sigma H has been clearly demonstrated (9, 15, 19), but the detailed features of the mechanism remain to be elucidated. Liu et al. (35) have recently reported that sigma H is subject to additional levels of posttranslational control involving the ATP-dependent protease Lon and the regulatory ATPase ClpX. Ohashi et al. (40) have recently isolated spo0H(Ts) mutants in which the level of sigma H is decreased at the nonpermissive temperature. Based on the finding that mutations within rpoB encoding the beta  subunit of RNA polymerase restore the level of sigma H in these temperature-sensitive mutants, they suggest that holoenzyme formation contributes to the stabilization of sigma H at the start of sporulation (40). Our observation that an increased level of internal Na+ may affect posttranscriptional regulation of sigma H leads us to speculate that a reaction(s) or interaction(s) sensitive to Na+ may be involved in the posttranscriptional regulation of sigma H. It seems that sigma H is unable to stably associate with RNA polymerase to form the holoenzyme under conditions in which the internal Na+ concentration is high. Alternatively, the impaired accumulation of sigma H in the shaA mutant may be the consequence of abnormal induction of the Lon- or ClpX-dependent proteolytic regulation of sigma H under conditions in which the internal Na+ concentration is high.

There is another report that offers an important suggestion concerning posttranscriptional control of the sigma H protein. Cosby and Zuber (9) showed that an increase in the pH of the culture medium from ~5 to 7 results in an increase in the level of expression of sigma H-dependent genes and, thus, an increase in the level of sigma H protein, whereas spo0H transcription is not fully increased, under conditions in which glucose and glutamine are present in excess. An active tricarboxylic acid (TCA) cycle seems to be required for the induction of sigma H-dependent gene expression caused by elevation of the pH (9). It is unknown, however, how elevation of the external pH causes an increase in the internal sigma H level. We think that the increase in pH itself rather than the absolute value of external pH may be important in stabilization of sigma H based on the following considerations. Cytoplasmic pH is not maintained entirely constant, but it changes far less than the external pH; that is, there is substantial pH homeostasis (3, 32, 43). Since the magnitude of the proton motive force (Delta p) is relatively independent of external pH, the composition of Delta p (the chemical component Delta pH versus the electrical component Delta Psi ) has to change in response to external pH to provide such pH homeostasis (43). Under acidic conditions, a large inwardly directed Delta pH mainly contributes to Delta p (30). Thus, it is likely that transient elevation of the external pH under the above conditions would result in a decrease in Delta pH, and, therefore, Delta p. As a consequence, the TCA cycle and respiration would be activated in order to support recovery of the Delta p. The improved Delta p would activate several secondary membrane transporters, including Na+/H+ antiporters, and thereby change internal ion levels, which would be expected to result in the stabilization of sigma H. Thus, the results reported by Cosby and Zuber (9) indicating that an increase in external pH results in stabilization of sigma H and our results indicating that disruption of shaA leads to an increased level of internal Na+ which affects the accumulation of sigma H may raise the possibility that posttranscriptional regulation of sigma H is influenced by internal ion levels, including the internal pH and/or the internal Na+ concentration.

Many studies on bacterial Na+/H+ antiporters have focused on pH homeostasis or sodium extrusion during vegetative growth. We have shown here that the function of the ShaA antiporter is required for the initiation of sporulation. Considering that a subtle change in internal pH and/or ion concentrations can alter cellular reactions or interactions, fine control of cytoplasmic ion levels is likely to be very important for cellular functions that involve many complicated reactions and steps, such as sporulation.

It has been shown recently that shaA and the six genes adjacent to it are transcribed as an operon (25). According to our preliminary findings in primer extension analyses, three bands of reverse transcripts were detected, suggesting that there are three transcriptional start sites in the regulatory region of the sha operon (unpublished results). In the region upstream of each start site, two apparent sigma A-dependent promoters and a sigma B-like promoter were found. However, the primer-extended band corresponding to the sigma B-like promoter did not disappear in the case of a sigB-null mutant, suggesting that expression of the sha operon may depend on other sigma factors, such as the extracytoplasmic function-type sigma factors (36). The regulatory mechanism controlling transcription of the sha operon during both vegetative growth and sporulation is the subject of future study.


    ACKNOWLEDGMENTS

We are grateful to Roy H. Doi for critical reading of the manuscript. We are also grateful to Abraham L. Sonenshein for helpful suggestions.

This work was partially supported by a grant for the Biodesign Research Program from RIKEN to S.K., M.K., and T.K. and by a Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Science, and Culture of Japan to F.K.


    FOOTNOTES

* Corresponding author. Mailing address: Microbiology Laboratory, Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. Phone: 81-48-467-9545. Fax: 81-48-462-4672. E-mail: kosono{at}postman.riken.go.jp.

dagger Present address: National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan.


    REFERENCES
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

1. Asai, K., M. Fujita, F. Kawamura, H. Takahashi, Y. Kobayashi, and Y. Sadaie. 1998. Restricted transcription from sigma H or phosphorylated Spo0A dependent promoters in the temperature-sensitive secA341 mutant of Bacillus subtilis. Biosci. Biotechnol. Biochem. 62:1707-1713[CrossRef][Medline].
2. Asai, K., F. Kawamura, H. Yoshikawa, and H. Takahashi. 1995. Expression of kinA and accumulation of sigma H at the onset of sporulation in Bacillus subtilis. J. Bacteriol. 177:6679-6683[Abstract/Free Full Text].
3. Booth, I. R. 1985. Regulation of cytoplasmic pH in bacteria. Microbiol. Rev. 49:359-378[Free Full Text].
4. Burbulys, D., K. A. Trach, and J. A. Hoch. 1991. The initiation of sporulation in Bacillus subtilis is controlled by a multicomponent phosphorelay. Cell 64:545-552[CrossRef][Medline].
5. Charney, J., W. P. Fisher, and C. P. Hegarty. 1951. Manganese as an essential element for sporulation in the genus Bacillus. J. Bacteriol. 62:145-148[Free Full Text].
6. Cheng, J., A. A. Guffanti, and T. A. Krulwich. 1994. The chromosomal tetracycline resistance locus of Bacillus subtilis encodes a Na+/H+ antiporter that is physiologically important at elevated pH. J. Biol. Chem. 269:27365-27371[Abstract/Free Full Text].
7. Chibazakura, T., F. Kawamura, and H. Takahashi. 1991. Differential regulation of spo0A transcription in Bacillus subtilis: glucose represses promoter switching at the initiation of sporulation. J. Bacteriol. 173:2625-2632[Abstract/Free Full Text].
8. Chung, J. D., G. Stephanopoulos, K. Ireton, and A. D. Grossman. 1994. Gene expression in single cells of Bacillus subtilis: evidence that a threshold mechanism controls the initiation of sporulation. J. Bacteriol. 176:1977-1984[Abstract/Free Full Text].
9. Cosby, W. M., and P. Zuber. 1997. Regulation of Bacillus subtilis sigma H (Spo0H) and AbrB in response to changes in external pH. J. Bacteriol. 179:6778-6787[Abstract/Free Full Text].
10. Dubnau, D., and R. Davidoff-Abelson. 1971. Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex. J. Mol. Biol. 14:209-221.
11. Duncan, L., S. Alper, F. Arigoni, R. Losick, and P. Stragier. 1995. Activation of cell-specific transcription by a serine phosphatase at the site of asymmetric division. Science 270:641-644[Abstract/Free Full Text].
12. Fisher, S. H., and A. L. Sonenshein. 1991. Control of carbon and nitrogen metabolism in Bacillus subtilis. Annu. Rev. Microbiol. 45:107-135[CrossRef][Medline].
13. Fortnagel, P., and E. Freese. 1968. Inhibition of aconitase by chelation of transition metals causing inhibition of sporulation in Bacillus subtilis. J. Biol. Chem. 243:5289-5295[Abstract/Free Full Text].
14. Freese, E. B., N. Vasantha, and E. Freese. 1979. Identification in developmental mutants of Bacillus subtilis. Mol. Gen. Genet. 170:67-74[Medline].
15. Frisby, D., and P. Zuber. 1994. Mutations in pts cause catabolite-resistant sporulation and altered regulation of spo0H in Bacillus subtilis. J. Bacteriol. 176:2587-2595[Abstract/Free Full Text].
16. Grimshaw, C. E., S. Huang, C. G. Hanstein, M. A. Strauch, D. Burbulys, L. Wang, J. A. Hoch, and J. M. Whiteley. 1998. Synergistic kinetic interactions between components of the phosphorelay controlling sporulation in Bacillus subtilis. Biochemistry 37:1365-1375[CrossRef][Medline].
17. Grinstein, S., M. Woodside, C. Sardet, J. Pouyssegur, and D. Rotin. 1992. Activation of the Na+/H+ antiporter during cell volume regulation. Evidence for a phosphorylation-independent mechanism. J. Biol. Chem. 267:23823-23828[Abstract/Free Full Text].
18. Hamamoto, T., M. Hashimoto, M. Hino, M. Kitada, Y. Seto, T. Kudo, and K. Horikoshi. 1994. Characterization of a gene responsible for the Na+/H+ antiporter system of alkaliphilic Bacillus species strain C-125. Mol. Microbiol. 14:939-946[CrossRef][Medline].
19. Healy, J., J. Weir, I. Smith, and R. Losick. 1991. Post-transcriptional control of a sporulation regulatory gene encoding factor sigma H in Bacillus subtilis. Mol. Microbiol. 5:477-487[CrossRef][Medline].
20. Hiramatsu, T., K. Kodama, T. Kuroda, T. Mizushima, and T. Tsuchiya. 1998. A putative multisubunit Na+/H+ antiporter from Staphylococcus aureus. J. Bacteriol. 180:6642-6648[Abstract/Free Full Text].
21. Hirata, H., T. Fukazawa, S. Negoro, and H. Okada. 1986. Structure of a beta -galactosidase gene of Bacillus stearothermophilus. J. Bacteriol. 166:722-727[Abstract/Free Full Text].
22. Hirata, H., S. Negoro, and H. Okada. 1985. High production of thermostable beta -galactosidase of Bacillus stearothermophilus in Bacillus subtilis. Appl. Environ. Microbiol. 49:1547-1549[Abstract/Free Full Text].
23. Hoch, J. A. 1993. Regulation of the phosphorelay and the initiation of sporulation in Bacillus subtilis. Annu. Rev. Microbiol. 47:441-465[CrossRef][Medline].
24. Hoch, J. A., K. Trach, F. Kawamura, and H. Saito. 1985. Identification of the transcriptional suppressor sof-1 as an alteration in the spo0A protein. J. Bacteriol. 161:552-555[Abstract/Free Full Text].
25. Ito, M., A. A. Guffanti, B. Oudega, and T. A. Krulwich. 1999. mrp, a multigene, multifunctional locus in Bacillus subtilis with roles in resistance to cholate and to Na+ and in pH homeostasis. J. Bacteriol. 181:2394-2402[Abstract/Free Full Text].
26. Ivey, D. M., A. A. Guffanti, J. S. Bossewitch, E. Padan, and T. A. Krulwich. 1991. Molecular cloning and sequencing of a gene from alkaliphilic Bacillus firmus OF4 that functionally complements an Escherichia coli strain carrying a deletion in the nhaA Na+/H+ antiporter gene. J. Biol. Chem. 266:23483-23489[Abstract/Free Full Text].
27. Ivey, D. M., A. A. Guffanti, J. Zemsky, E. Pinner, R. Karpel, S. Schuldiner, E. Padan, and T. A. Krulwich. 1993. Cloning and characterization of a putative Ca2+/H+ antiporter gene from Escherichia coli upon functional complementation of Na+/H+ antiporter-deficient strains by the overexpressed gene. J. Biol. Chem. 268:11296-11303[Abstract/Free Full Text].
28. Kawamura, F., and H. Saito. 1983. Isolation and mapping of a new suppressor mutation of an early sporulation gene spo0F mutation in Bacillus subtilis. Mol. Gen. Genet. 192:330-334[CrossRef][Medline].
29. Kawano, M., K. Igarashi, and Y. Kakinuma. 1998. The Na+-responsive ntp operon is indispensable for homeostasis of K+ and Na+ in Enterococcus hirae at limited proton potential. J. Bacteriol. 180:4942-4945[Abstract/Free Full Text].
30. Khan, S., and R. M. Macnab. 1980. Proton chemical potential, proton electrical potential and bacterial motility. J. Mol. Biol. 138:599-614[CrossRef][Medline].
31. Kosono, S., S. Morotomi, M. Kitada, and T. Kudo. 1999. Analyses of a Bacillus subtilis homologue of the Na+/H+ antiporter gene which is important for pH homeostasis of alkaliphilic Bacillus sp. C-125. Biochim. Biophys. Acta 1409:171-175[Medline].
32. Krulwich, T. A., J. Cheng, and A. A. Guffanti. 1994. The role of monovalent cation/proton antiporters in Na+-resistance and pH homeostasis in Bacillus: an alkaliphile versus a neutralophile. J. Exp. Biol. 196:457-470[Abstract/Free Full Text].
33. Lanyi, J. K. 1979. The role of Na+ in transport processes of bacterial membrane. Biochim. Biophys. Acta 559:377-397[Medline].
34. Leighton, T. J., and R. H. Doi. 1971. The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis. J. Biol. Chem. 246:3189-3195[Abstract/Free Full Text].
35. Liu, J., W. M. Cosby, and P. Zuber. 1999. Role of Lon and ClpX in the post-translational regulation of a sigma subunit of RNA polymerase required for cellular differentiation in Bacillus subtilis. Mol. Microbiol. 33:415-428[CrossRef][Medline].
36. Missiakas, D., and S. Raina. 1998. The cytoplasmic function sigma factors: role and regulation. Mol. Microbiol. 28:1059-1066[CrossRef][Medline].
37. Mueller, J. P., and A. L. Sonenshein. 1992. Role of the Bacillus subtilis gsiA gene in regulation of early sporulation gene expression. J. Bacteriol. 174:4374-4383[Abstract/Free Full Text].
38. Nanamiya, H., Y. Ohashi, K. Asai, S. Moriya, N. Ogasawara, M. Fujita, Y. Sadaie, and F. Kawamura. 1998. ClpC regulates the fate of a sporulation initiation sigma factor, sigma H protein, in Bacillus subtilis at elevated temperatures. Mol. Microbiol. 29:505-513[CrossRef][Medline].
39. Nozaki, K., T. Kuroda, T. Mizushima, and T. Tsuchiya. 1998. A new Na+/H+ antiporter, NhaD, of Vibrio parahaemolyticus. Biochim. Biophys. Acta 1369:213-220[Medline].
40. Ohashi, Y., K. Sugimaru, H. Nanamiya, T. Sebata, K. Asai, H. Yoshikawa, and F. Kawamura. 1999. Thermo-labile stability of sigma H (Spo0H) in temperature-sensitive spo0H mutants of Bacillus subtilis can be suppressed by mutations in RNA polymerase beta  subunit. Gene 229:117-124[CrossRef][Medline].
41. Padan, E., N. Maisler, D. Taglicht, R. Karpel, and S. Schuldiner. 1989. Deletion of ant in Escherichia coli reveals its function in adaptation to high salinity and an alternative Na+/H+ antiporter system(s). J. Biol. Chem. 264:20297-20302[Abstract/Free Full Text].
42. Padan, E., and S. Schuldiner. 1994. Molecular physiology of Na+/H+ antiporters, key transporters in circulation of Na+ and H+ in cells. Biochim. Biophys. Acta 1185:129-151[Medline].
43. Padan, E., D. Zilberstein, and S. Schuldiner. 1981. pH homeostasis in bacteria. Biochim. Biophys. Acta 650:151-166[Medline].
44. Pinner, E., E. Padan, and S. Schuldiner. 1994. Kinetic properties of NhaB, a Na+/H+ antiporter from Escherichia coli. J. Biol. Chem. 269:26274-26279[Abstract/Free Full Text].
45. Putnoky, P., A. Kereszt, T. Nakamura, G. Endre, E. Grosskopf, P. Kiss, and A. Kondorosi. 1998. The pha gene cluster of Rhizobium meliloti involved in pH adaptation and symbiosis encodes a novel type of K+ efflux system. Mol. Microbiol. 28:1091-1101[CrossRef][Medline].
46. Scribner, H. E., J. Mogelson, E. Eisenstadt, and S. Silver. 1975. Regulation of cation transport during bacterial sporulation, p. 346-355. In P. Gerhardt, R. N. Costilow, and H. L. Sadoff (ed.), Spores VI. American Society for Microbiology, Washington, D.C.
47. Strauch, M. A., K. A. Trach, J. Day, and J. A. Hoch. 1992. Spo0A activates and represses its own synthesis by binding at its dual promoters. Biochimie 74:619-626[Medline].
48. Strauch, M. A., J. J. Wu, R. H. Jonas, and J. A. Hoch. 1993. A positive feedback loop controls transcription of the spo0F gene, a component of the sporulation phosphorelay in Bacillus subtilis. Mol. Microbiol. 7:967-974[Medline].
49. Taglicht, D., E. Padan, and S. Schuldiner. 1991. Overproduction and purification of a functional Na+/H+ antiporter coded by nhaA (ant) from Escherichia coli. J. Biol. Chem. 266:11289-11294[Abstract/Free Full Text].
50. Trach, K. A., and J. A. Hoch. 1993. Multisensory activation of the phosphorelay initiating sporulation in Bacillus subtilis: identification and sequence of the protein kinase of the alternate pathway. Mol. Microbiol. 8:69-79[Medline].
51. Tzeng, Y.-L., V. A. Feher, J. Cavanagh, M. Perego, and J. A. Hoch. 1998. Characterization of interactions between a two-component response regulator, Spo0F, and its phosphatase, RapB. Biochemistry 37:16538-16545[CrossRef][Medline].
52. Utsugi, J., K. Inaba, T. Kuroda, M. Tsuda, and T. Tsuchiya. 1998. Cloning and sequencing of a novel Na+/H+ antiporter gene from Pseudomonas aeruginosa. Biochim. Biophys. Acta 1398:330-334[Medline].
53. Waser, M., D. Hess-Bienz, K. Davies, and M. Solioz. 1992. Cloning and disruption of a putative Na+/H+ antiporter gene of Enterococcus hirae. J. Biol. Chem. 267:5396-5400[Abstract/Free Full Text].
54. Weir, J., M. Predich, E. Dubnau, G. Nair, and I. Smith. 1991. Regulation of spo0H, a gene coding for the Bacillus subtilis sigma H factor. J. Bacteriol. 173:521-529[Abstract/Free Full Text].
55. West, I. C., and P. Mitchell. 1974. Proton/sodium ion transport in Escherichia coli. Biochem. J. 144:87-90[Medline].

Journal of Bacteriology, February 2000, p. 898-904, Vol. 182, No. 4
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