INTRODUCTION

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New therapies are urgently needed to treat Mycobacterium tuberculosis, one of the leading causes of death from a single infectious disease worldwide (15). Although this pathogen can be treated effectively with multidrug therapy (1), incomplete treatment has led to the development of drug-resistant strains (17, 30). In general, antibiotics that inhibit cell wall biosynthesis are among the most effective agents against bacterial pathogens. In the case of M. tuberculosis, a number of highly effective antibiotics including isoniazid (INH) and ethambutol work by inhibiting biosynthesis of the mycobacterial cell wall. Because the cell wall is an attractive target for antibiotic development, considerable effort has been focused on discovering the metabolic steps that are essential for biosynthesis of cell wall components (10, 11, 23, 28). The enzymatic targets of many cell wall active antibiotics also have been discovered recently (7, 12, 25, 38).

Despite progress in characterizing the mycobacterial cell wall, very little is known about the intracellular mechanisms that are activated as a consequence of cell wall injury. Antibiotic-induced cell death may involve events that are not directly related to inhibition of the primary antibiotic target. For example, autolysins are activated when cell wall biosynthesis is inhibited by -lactam antibiotics in many gram-positive bacteria (13, 19, 39). Microarray technology has revealed that diverse sets of genes are induced following treatment of cells with drugs (14, 42). In the case of M. tuberculosis, novel antibiotics that directly target these pathways may be highly effective in the treatment of disease due to this organism, either alone or synergistically with other cell wall-active agents. The identification of promoters that are specifically induced by cell wall damage could also be valuable as part of a screen that used reporter assays to discover novel cell wall-active compounds (25).

In the course of studying the differential gene expression of M. tuberculosis in response to INH, we discovered three INH-induced genes organized in tandem on the M. tuberculosis genome that we termed iniB, iniA, and iniC (3). We postulated that all three ini genes comprised a single operon, iniBAC. Northern blot and reverse transcription-PCR experiments demonstrated that the iniA gene was induced by both INH and ethambutol (3), two antibiotics that act on the cell wall by different mechanisms (7, 12, 25, 38). The kasA gene has been shown to be induced by INH and the related compound ethionamide (25, 42). However, no mycobacterial promoters that are induced by unrelated antibiotics targeting different pathways of cell wall biosynthesis have been identified. Little is known about the structure of regulated promoters in M. tuberculosis. The inducible promoters that have been characterized in M. tuberculosis include promoters for the sigma factors sigB (20) and sigF (27), the DNA repair protein recA (26), and the response regulator mtrA (41). In the case of recA, a putative upstream activation sequence has been identified, and a Cheo-like box that binds to the transcriptional repressor LexA has been found (26). Here, we demonstrate that the promoter of the putative iniBAC operon is specifically induced by a broad range of inhibitors to cell wall biosynthesis including antibiotics that inhibit the synthesis of (i) peptidoglycan (ampicillin and ampicillin/sulbactam [24]), (ii) arabinogalactam (ethambutol [12, 38]), (iii) mycolic acids (INH, ethionamide, and 2-alkynoic acid [KOAs]), and (iv) fatty acids (5-chloropyrazinamide [5-chloro-PZA]) (7, 25; O. Zimhony et al., unpublished data). We characterize the nature of the induction and demonstrate the suitability of the promoter to screen for cell wall-active antibiotics using luciferase reporter plasmids. The structure of the promoter is investigated and likely regulatory sequences are identified.

	MATERIALS AND METHODS

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Bacteria and culture methodology. Escherichia coli DH5 (33) was used for all plasmid constructions. E. coli cultures were grown at 37°C in Luria-Bertani medium (33) with the addition of hygromycin B (200 µg/ml; Sigma, St. Louis, Mo.) or kanamycin (40 µg/ml; Sigma) where appropriate. Mycobacterium bovis BCG Pasteur and BCG Montreal strains ATCC 35735 and ATCC 35747, and M. smegmatis strain mc²155 (34) were grown at 37°C on a rotary shaker in Middlebrook 7H9 medium (Difco, Detroit, Mich.) containing 0.05% Tween 80, 0.02% glycerol, and 10% oleic-albumin dextrose complex (Becton Dickinson, Cockeysville, Md.), with the addition of hygromycin B (50 µg/ml) for strains containing pYUB509-based constructs or kanamycin (24 µg/ml) for mc²155 strains containing pCV125-based constructs. For induction experiments, additional antibiotics were added at the indicated final concentrations. BCG and mc²155 strains were cultured in 150-cm² tissue culture flasks (Corning, Cambridge, Mass.) at 100 ml per flask, starting from 1:100 dilutions of strain stocks. Cultures were grown to an optical density at 590 nm (OD₅₉₀) of approximately 0.4, except for experiments specifically designed to examine promoter induction at various ODs. The cultures were then split into 30-ml square medium bottles (Nalgene, Rochester, N.Y.) at 5 ml per bottle, antibiotics and other reagents were added to the growing cultures as indicated, and the cultures were then returned to the incubator. Culture aliquots were removed at specified time points for luciferase or -galactosidase assays.

Sequence positions. The sequence numbering used in this study corresponds to the M. tuberculosis genomic sequence position (16; National Center for Biotechnology Information [NCBI] database [http://www.ncbi.nlm.nih.gov/cgi-bin/Entrez/framik?db=Genome&gi=135]). By this convention, the iniB gene (Rv0341 or MTCY13E10.01) starts at 409354, the iniA gene (Rv0342 or MTCY13E10.02) starts at 410824, and the iniC gene (Rv0343 or MTCY13E10.03) starts at 412755. The translational start sites designated in this investigation vary slightly from previous annotations.

Plasmids and strains. The plasmids and strains used in this study are listed in Table 1. Plasmid pYUB509, containing lacZ and fflux reporter genes, was used to construct pG4697-6 and pG1697-3 for testing iniBAC promoter activity in luciferase and -galactosidase assays (Table 1). Plasmid pG4697-6, which contained a sequence beginning 211 bp upstream of the first iniBAC open reading frame (iniB) extending to the translational start site, was transformed into the antibiotic-susceptible BCG Montreal strain ATCC 35735 (BCG^S) to create strain BCG^S(pG4697-6). Plasmid pG4697-6 was also transformed into the INH-resistant BCG Montreal strain ATCC 35747 (BCG^R), which contains a deletion in the katG gene, to create strain BCG^R(pG4697-6). Plasmid pG1697-3, which contained the same 211-bp region in the reverse orientation, was transformed into BCG^S to create strain BCG^S(pG1697-3). Plasmid pCV125, an integrating vector containing kanamycin resistance and a promoterless lacZ gene, was obtained from MedImmune (Gaithersburg, Md.). This plasmid was used as the basis for constructs aimed at testing the activity of partial promoter deletions. Fourteen plasmids were constructed by ligating successive 5' or 3' deletions of the 211-bp sequence upstream of iniB into pCV125 (Table 1). These 14 plasmids were transformed into M. smegmatis strain mc²155 to create strains mc²155(pG4799-1) through mc²155(pG4799-12), mc²155(pG15499-1), and mc²155(pG15499-2). The finished plasmid constructs were subjected to automated DNA sequencing in order to exclude mutations that could occur during the PCR or cloning process. The unpublished plasmid pKB15 was a gift of Graham Hatfull, this integrating plasmid carries the fflux gene under control of the L5 phage p_L promoter, resulting in constitutive expression of luciferase. Plasmid pKB15 also contains hygromycin and ampicillin resistance genes and L5 attP, int, and oriE. pKB15 was transformed into BCG Pasteur, resulting in strain BCG(pKB15).

Chromosomal DNA and RNA extraction. Chromosomal DNA from different mycobacterial species was extracted using a sodium dodecyl sulfate (SDS)-hexadecyltrimethylammonium bromide (Fisher, Pittsburgh, Pa.) protocol as described previously (2). For RNA preparation, BCG strain ATCC 35735 was grown to an OD₅₉₀ of 0.5. INH was then added to the culture for a final concentration of 1.0 µg/ml, or the culture was allowed to continue without added INH. After an additional 18-h incubation, RNA was extracted using a TRIzol (Life Technologies, Gaithersburg, Md.) based protocol as described previously (3).

PCR generation of amplicons. PCRs were performed in 50-µl volumes containing either 10 ng of chromosomal DNA or 1 ng of plasmid DNA with 2.5 mM each deoxynucleosides triphosphate, 20 pmol each of upstream and downstream primers, 1.25 U of Taq polymerase with a final concentration of 1× PCR buffer (Gibco BRL, Grand Island, N.Y.), and 2 mM MgCl₂. DNA was amplified in an Applied Biosystems Geneamp 9700 thermal cycler (Perkin-Elmer, Foster City, Calif.) for 30 cycles of 94°C for 1 min, annealing at 55°C for 1 min, and 72°C for 1 min, followed by 72°C for 10 min.

Luciferase assays. At specified time points, 25 µl of each culture was removed and added to 75 µl of 7H9 medium in a glass cuvette (Lumacuvette-P; Celsis-Lumac, Landgraaf, The Netherlands). Luciferase activity was measured using 40 mM luciferin (Sigma) in 1 M C₆H₅Na₃O₇ · 2H₂O (pH 4.5) in a Lumac 2010A luminometer (Celsis-Lumac) according to the manufacturer's recommendations. Induction was calculated as follows: relative light units (RLU) for sample culture/RLU for medium-only control culture, if RLU for sample > RLU for control. A decrease in luciferase activity of the sample culture compared to the control culture (repression) was calculated as RLU for control/RLU for sample.

-Galactosidase assays. At specified time points, 500 µl of each culture was set aside on ice for subsequent measurement of the OD₅₉₀. An additional 500 µl of each culture was simultaneously removed and added to 500 µl of Z buffer (60 mM Na₂HPO₄ · 7H₂O, 40 mM NaH₂PO₄ · H₂O, 10 mM KCl, 1 mM MgSO₄ · 7H₂O, 50 mM -mercaptoethanol, adjusted to pH 7.0) in a 2-ml microcentrifuge tube. Two drops of chloroform and one drop of 0.1% SDS were then added, and the tubes were vortexed for 30 s. The tubes were incubated at 28°C for 5 min, 200 µl of fresh o-nitrophenyl--D-galactopyranoside (ONPG) (4 mg/ml; Sigma) in Z buffer was added to each tube, and the tubes were shaken well and incubated at 28°C. When a faint yellow color appeared in the control tube (5 to 20 min), the reaction was stopped with 0.5 ml of 1 M Na₂CO₃ and spun in a microcentrifuge at top speed for 5 min, and the OD₄₂₀ of the supernatant was measured. -Galactosidase units were calculated using the formula 1,000 × OD₄₂₀/time (minutes) × 0.5 × OD₅₉₀.

Primer extension. Oligonucleotide primers were end labeled with [³²P]ATP at their 5' ends, using T4 polynucleotide kinase as described in the primer extension kit (Promega, Madison, Wis.); 0.1 pmol of labeled primer was annealed to 6.5 µg of total RNA at 75°C for 0.5 h. Extension was carried out with avian myeloblastosis virus reverse transcriptase (Promega) according to the manufacturer's instructions at 42°C for 0.5 h. The reactions were added to 20 µl of loading dye (98% [vol/vol] formamide, 10 mM EDTA, 0.1% xylene cyanol, 0.1% bromophenol blue) and denatured at 90°C for 10 min. The reaction products were then run on an 8% polyacrylamide-urea sequencing gel. Bands were visualized by autoradiography. Sequencing reactions were carried out by cycle sequencing (Perkin-Elmer) according to the manufacturer's instructions.

Southern blots. Genomic DNA from different mycobacterial species was digested with PvuII, subjected to electrophoresis in a 0.7% agarose gel, and transferred by capillary action to Biotrans Plus nylon membranes (ICN Pharmaceuticals, Costa Mesa, Calif.). The blots were prehybridized at 50°C in Rapid-Hyb buffer (Amersham, Arlington Heights, Ill.) and then hybridized overnight with [³²P]dCTP-radiolabeled (Megaprime labeling kit; Amersham) probes. The probe complementary to a 400-bp segment of the iniA gene was generated by PCR using the primers iniART-T and iniART-B. The probe complementary to the entire iniB gene was generated by a BamHI-NruI digestion of pG7897-4 (Table 1). The blots were washed in progressively more stringent conditions until autoradiography revealed clear bands and minimal background hybridization.

Statistical analysis. Mean induction or repression and 95% confidence internals were calculated using Microsoft Excel 97 software.

	RESULTS

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Induction of the iniBAC promoter. The discovery that the iniA gene was induced by ethambutol as well as INH (3) suggested that induction was not specific to inhibition of mycolic acid biosynthesis. Integrating reporter plasmids containing the iniBAC promoter fused to the genes encoding luciferase and -galactosidase were constructed to further investigate the induction characteristics. The iniA gene appeared to be the second gene in a three-gene operon consisting of iniB, iniA, and iniC. We chose to investigate the promoter activity of the sequence extending 211 bp upstream of iniB to the translational start site of iniB (positions 409142 to 409353). BCG^S(pG4697-6), which contained the full-length 211-bp sequence, was cultured to log phase and then split into untreated portions or portions that were treated with antibiotics and other reagents. Induction was assessed by comparing luciferase activity in the treated portions to that in the untreated control. Significant induction occurred in the presence of many different cell wall-active agents despite the divergence of their known mechanisms of action (Fig. 1A). After 24 and 48 h of incubation with antibiotics, induction by INH, ethambutol, ethionamide, 5-chloro-PZA, and KOA was 10- to 30-fold greater than control cultures. Induction by ampicillin, and by the combination -lactam--lactamase inhibitor Unasyn (ampicillin/sulbactam), was consistently three- to fivefold greater than in control cultures. Induction by INH was reversed by coincubation with rifampin, indicating that the induction was due to increased rates of transcription. Among the cell wall biosynthesis inhibitors tested, only cycloserine did not result in induction of the reporter.

View larger version (3619K): [in this window] [in a new window]   FIG. 1.   Effects of different compounds on iniBAC promoter activity as measured by luciferase assays of integrated transcriptional fusion plasmids. Induction is shown after incubation with compounds for 24 h (grey bars) or 48 h (black bars). Final concentrations are indicated in micrograms per milliliter. Error bars represent 95% confidence intervals. (A) INH-susceptible BCGS(pG4697-6) containing the iniBAC promoter fused to lacZ and fflux genes [-Pro indicates assays performed with BCGS(pG1697-3), which contains the same construct, except that the transcriptional fusion was performed by inserting the promoter in the opposite orientation from the coding region]. (B) INH-susceptible BCG(pMKB15) containing the L5 phage pL promoter fused to the fflux gene. (C) INH-resistant BCGR(pG4697-6) containing the iniBAC promoter fused to lacZ and fflux genes.

View larger version (13K): [in this window] [in a new window]   FIG. 2.   Induction of the iniBAC promoter in BCGS(pG4697-6) after incubation with INH for 24 () and 48 () h and with ethambutol for 24 () and 48 () h.

Induction kinetics. The time course of iniBAC promoter induction after incubation with antibiotics was tested by incubating BCG^S(pG4697-6) with INH (1 µg/ml) or ethambutol (5 µg/ml) and assessing serial aliquots for luciferase activity. Induction was apparent as early as 4 h after incubation with either antibiotic, reaching a maximum between 25 and 48 h (Fig. 3).

View larger version (12K): [in this window] [in a new window]   FIG. 3.   Induction of the iniBAC promoter in BCG strain BCGS(pG4697-6) after incubation with INH (1 µg/ml; ) and ethambutol (5 µg/ml; ) as a function of incubation time.

View larger version (38K): [in this window] [in a new window]   FIG. 4.   Induction in different phases of growth. BCGS(pG4697-6) was subcultured by performing a 1:100 dilution of an actively growing culture. Serial aliquots were removed at increasing OD590 and split into paired subcultures. One subculture of each pair was not treated with antibiotics (); the other subculture of each pair was treated with ethambutol at a final concentration of 5 µg/ml (). After an additional 24 h, -galactosidase activity was measured. -Galactosidase units are shown as a function of the OD590 of the paired subcultures at the time that they were removed from the parent culture.

Species distribution of the iniBAC operon. We previously determined that INH induced the iniA genes from both M. tuberculosis H37Rv and BCG (3). The 211-bp promoter region was sequenced in BCG strain ATCC 35735. Alignment with the corresponding sequence in M. tuberculosis strain H37Rv (16; NCBI database) revealed that the two sequences were 100% identical. Because mycobacterial species have similar cell wall structures, the presence of the iniBAC operon in different species of mycobacteria was assessed. Two probes were used, one complementary to a 400-bp region of the iniA gene and the other complementary to the entire iniB gene. The radiolabeled probes were hybridized separately to Southern blots containing genomic digests of three M. tuberculosis strains (Erdman, H37Rv, and H37Ra), of mycobacterial strain BCG, and of M. smegmatis (strain mc²155), M. avium, M. marinum, M. microti, and M. nonchromogenicum. The iniA probe hybridized strongly to single bands in all of the species except M. avium and M. nonchromogenicum. Hybridization to the M. nonchromogenicum genomic DNA resulted in two weak bands (Fig. 5). The iniB probe also failed to hybridize to M. avium, although it hybridized strongly to all of the other species except M. marinum. A control probe complementary to a region of the M. tuberculosis 16S rRNA gene hybridized to all of the species tested.

View larger version (81K): [in this window] [in a new window]   FIG. 5.   Testing of the iniBAC operon in different mycobacterial species. Mycobacterial species were tested by low-stringency Southern blot hybridization for the presence of the iniA and iniB genes: M. marinum (lane 1), H37ra (lane 2), M. tuberculosis strains Erdman (lane 3) and H37Rv (lane 4), M. avium (lane 5), BCG (lane 6), M. smegmatis (lane 7), M. nonchromogenicum (lane 8), and M. microti (lane 9). (A) Agarose gel showing PvuII digests of chromosomal DNA. (B) Southern blot of the gel in panel A hybridized with the iniA gene probe. (C) Hybridization with the M. tuberculosis 16S RNA gene probe. (D) Hybridization with the iniB gene.

Mapping the transcriptional start site of the BCG iniBAC operon. The transcriptional start site for the operon was determined by primer extension analysis in order to better understand the structure of the promoter region. Total RNA was extracted from BCG^S grown in the presence or absence of INH during the last 18 h of culture. Primer extension was performed with both samples using iniBprimer ext-3, which is complementary to the first 21-bp of the translated M. tuberculosis iniB gene (Table 2). No products were visualized in the primer extensions of BCG RNA that had not been treated with isoniazid. A single product was detected after primer extension of RNA from isoniazid treated BCG. The band was situated 45 bp upstream of the likely translational start site (position 409308) (Fig. 6). Primer extensions performed with two additional primers complementary to other iniB gene sequences (iniBprimer ext-1 and iniBprimer ext-2) yielded identical results (not shown). The complete absence of product from the RNA that was not treated with INH was confirmed by prolonged exposure of the gels. This result is consistent with previously described Northern blot hybridizations of M. tuberculosis in which no iniA RNA was detected unless the samples were treated with INH (3).

View larger version (64K): [in this window] [in a new window]   FIG. 6.   Mapping the transcriptional start sites of the iniBAC operon. Primer extension experiments were performed with end-labeled iniBprimer ext-3 using either no RNA or RNA isolated from BCG cultured in the absence of INH (INH) or in the presence of INH at a final concentration of 1 µg/ml (INH+). Sequencing reactions were performed with the identical primer and run alongside the primer extension reactions.

Deletion analysis of the iniBAC promoter. The induction kinetics of the 211-bp M. tuberculosis iniBAC promoter region was investigated in M. smegmatis using strain mc²155(pG4799-12). This strain contained the full-length M. tuberculosis promoter region cloned into the integrating vector pCV125 upstream of lacZ. We observed that M. smegmatis had an induction phenotype similar to that of BCG, although higher concentrations of INH were required because M. smegmatis is relatively INH resistant. Induction by INH (100 µg/ml) and 5 ethambutol (5 µg/ml) was seen as early as 30 min after incubation with antibiotics and was maximal after 4 h of incubation. These conditions were used to investigate the amount of upstream DNA sequence that was required to induce the -galactosidase reporter. Inserts containing serial 19 to 25-bp 5' deletions of the 211-bp promoter region were cloned into pCV125 (Fig. 7A), and -galactosidase activity was measured. Induction by both antibiotics was preserved until a 22-bp region 169 to 147 bp upstream from the translational start site (409184 to 409206) was deleted. After deletion of this sequence, both induction and uninduced expression fell to levels of the promoterless plasmid control (Fig. 7B).

View larger version (32K): [in this window] [in a new window]   FIG. 7.   Effect of promoter deletions on antibiotic induction. Serial 5' and 3' deletions of the 211-bp sequence immediately upstream of the translational start site of iniB were fused to the lacZ gene in the integrating plasmid pCV125 and transformed into M. smegmatis strain mc2155. (A) Schematic of promoter deletions and induction after 4 h of treatment with either INH (100 µg/ml) or ethambutol (EMB; 5 µg/ml). The dotted line indicates the position of a 19-bp spacer that is unrelated to the sequence it replaced; induction represents mean results from at least three experiments. Numbering corresponds to the distance from the translational start site. (B) -Galactosidase activity of promoter deletions without (grey) or with (black) 4 h of incubation with INH (100 µg/ml). Values represent means of at least three experiments. Error bars represent 95% confidence intervals.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

The iniBAC operon encodes genes that are induced specifically by a broad range of antibiotics that inhibit cell wall biosynthesis. With the exception of cycloserine, the promoter was induced by all clinically relevant antibiotics that act on the M. tuberculosis cell wall. Other toxic stimuli did not induce the promoter, demonstrating the specificity of induction. Significant repression of luciferase activity was observed after treatment with a number of toxic agents that do not act by cell wall inhibition. It is likely that the decreased availability of intracellular ATP levels in the dying cells contributed to this effect.

With the exception of general stress responses, it is extremely uncommon for antibiotics with different mechanisms of action to induce the same set of genes in bacteria. VanA-type vancomycin resistance can be induced by different antibiotics that inhibit cell wall synthesis, possibly through the binding of peptidoglycan precursors in a two-component regulatory system (4, 22, 40). AmpC, the chromosomal -lactamase in gram-negative bacteria, is induced by different -lactam antibiotics. Induction is oppositely controlled by cytoplasmic concentrations of biosynthetic and degradative intermediates of murein metabolism (muropeptides) (21). However, VanA-type vancomycin resistance is also induced by cell wall hydrolytic enzymes such as lysozyme (40). We found that the iniBAC promoter was not induced by either lysozyme or granulysin, a molecule released by cytotoxic CD8⁺ lymphocytes that directly kills extracellular M. tuberculosis by altering the membrane integrity of the bacillus (35). The ampC gene is induced by different -lactam antibiotics, but these compounds are likely to have similar mechanisms of action. In contrast, the iniBAC promoter is induced by cell wall biosynthesis inhibitors that act on different components of the cell wall. It is possible that the iniBAC operon is induced by osmotic stress; however, we believe this to be unlikely. While antibiotics might lead to alterations in permeability of the bacterial cell wall and subsequent osmotic shock, both lysozyme and granulysin would also be expected to result in permeability changes. Neither of these compounds led to induction of the promoter, suggesting that a different mechanism is involved in induction.

The functions of the genes encoded by the iniBAC operon are unknown. The iniB gene has weak homology to cell wall structural proteins. The iniA and iniC genes are hypothetical proteins with no close homologs (3). Given the induction pattern of this operon, it is possible that these genes participate in the regulation of cell wall growth. It is also possible that the iniBAC operon has either a causal or a protective role in cell death, when killing is initiated by inhibition of cell wall biosynthesis. Induction of the iniBAC operon was not detectable until 4 h of incubation with antibiotics; in contrast, induction of the FAS-II complex and most of the other genes known to be INH induced occurs as early as 20 min after exposure to INH (42). The delay in iniBAC induction corresponds to the time required for INH to decrease the viability of M. tuberculosis in culture (37), suggesting a link between the iniBAC operon and cell death. The rapid induction kinetics of the FAS-II complex and other INH-induced genes closely parallel the repressive effect of INH on mycolate biosynthesis (37). This suggests that unlike the iniBAC operon, these genes are induced by events related to the initial binding of INH to its target. The genes of the iniBAC operon lack close homologs in nonmycobacterial species. However, the iniA and iniB genes were detectable by low-stringency hybridization in the fast-growing and avirulent M. smegmatis and in both virulent and avirulent slow-growing mycobacteria. It is intriguing that only M. avium did not hybridize to either gene probe. It remains possible that these genes are present in M. avium but have insufficient homology to be detected by this method.

Deletion studies of the region upstream of the coding sequences demonstrate that regulatory elements essential for gene expression are located in two regions, an upstream region 169 to 147 bp 5' from the translational start site (409184 to 409206) and a downstream region 65 to 39 bp 5' from the translational start site (409314 to 409288) (Fig. 8). The upstream region contains a 10-bp sequence flanked by 6-bp inverted repeats and part of two tandem 8-bp direct repeats. Deletion of this region resulted in decreased induction, suggesting the presence of UP elements, curved sequences, or bent sequences that could influence initiation of transcription (32). The downstream region includes both the transcriptional start site and a sequence with strong homology to 10 promoter sequences (9, 29). The decreased induction that resulted from deletion of this region is in agreement with observations in E. coli that changes (or deletions) in the 10 region and the region immediately downstream of the transcriptional start site strongly influence promoter strength (32). The observation that induction can be increased by deleting the 19-bp 3' end of this promoter region is intriguing and suggests possible binding of a transcriptional repressor downstream of the transcriptional start site. The increased induction is sequence specific and not due to a change in spacing because replacement of the deletion with a spacer sequence does not alter the increased induction that was observed. Database analysis of regulatory sites has shown that the region downstream of 30 binds repressors almost exclusively, whereas activators bind predominantly to positions between 80 and 30 (18). The possibility that a repressor protein binds to this region is currently being investigated. The repressor sequence also appears to include a ribosomal binding site. It is possible that a second, less apparent ribosomal binding site could exist upstream of this sequence.

View larger version (31K): [in this window] [in a new window]   FIG. 8.   Proposed organization of iniBAC promoter region. Regions found to be essential for induction are underlined with solid lines; the dotted underline indicates the sequence whose deletion leads to increased induction. Also shown are the transcriptional start site (arrowhead), 6-bp inverted repeats (solid arrows), 8-bp direct repeats (dotted arrows), the position of the primer iniBprimer ext-3 used in the primer extension experiments (striped arrow), and possible 10 sequence and ribosomal binding site (rbs). The asterisk marks an alternate translational start site as annotated by Cole et al. (16; NCBI database).

The reporter assays that we describe can be easily adapted to a 96-well plate or solid-phase format. These assays can be used for high-throughput screening of combinatorial libraries with the aim of discovering new classes of compounds that inhibit Mycobacterium cell wall biosynthesis. Assays that are specific for cell wall inhibition may be more useful at finding biologically active compounds than simple screens for inhibition or killing of M. tuberculosis. When testing for inhibitory or cidal compounds through repression of the constitutive luciferase reporter strain BCG(pKB15), highly effective drugs such as INH resulted in little inhibition of luciferase activity. Furthermore, these antibiotics were indistinguishable from relatively ineffective agents such as paraminosalicylic acid using the BCG(pKB15) assay (Fig. 1B).

In conclusion, the iniBAC operon is specifically induced by inhibitors of cell wall biosynthesis. Regulation of transcription is likely to be complex, involving both activator and repressor molecules. Further investigation of the regulatory elements of the iniBAC operon can potentially improve the understanding of the intracellular mechanisms that are activated by cell wall inhibition. Characterization of the proteins encoded by the iniB, iniA, and iniC genes may aid in the development of new antibiotics that may be effective alone or synergistically with other cell wall-active drugs.