INTRODUCTION

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The oxidation of hydrogen sulfide or sulfur to sulfuric acid is one of the major reactions of the global sulfur cycle and is mediated by various aerobic lithotrophic and anaerobic phototrophic sulfur-oxidizing bacteria. Paracoccus pantotrophus GB17 is an aerobic, gram-negative, neutrophilic facultatively autotrophic bacterium which grows with thiosulfate or molecular hydrogen as an energy source and heterotrophically with a large variety of carbon sources. P. pantotrophus, isolated as Thiosphaera pantotropha (36) and reclassified as Paracoccus denitrificans (26), was recently renamed (33). The gene region of P. pantotrophus coding for sulfur oxidation (Sox) is located on a 13-kb DNA fragment (29). The region comprises 12 open reading frames, i.e., ORF1, shxVW, and soxXYZABCDEF, with an incomplete soxF' at the end of the cloned DNA suggesting the extension of the sox gene cluster (18; F. Bardischewsky and C. G. Friedrich, submitted for publication) (Fig. 1).

View larger version (16K): [in this window] [in a new window]   FIG. 1.   Physical map of the relevant inserts of plasmids pEG12, pSOXF5N, and pVKB9 and of sox-relevant open reading frames and construction of the deletion in soxF.

The thiosulfate-oxidizing enzyme system of P. pantotrophus is located in the periplasm, and four proteins, i.e., SoxXA, SoxYZ, SoxB, and SoxCD, are required for the complete oxidation of thiosulfate (18). SoxXA is a heterodimeric c-type cytochrome, with SoxX (14,216 Da) as a monoheme subunit and SoxA (29,352 Da) as a diheme subunit. The heterodimeric SoxYZ protein is composed of SoxY (10,977 Da) and SoxZ (11,719 Da), neither of which contains a cofactor. The monomeric dimanganese SoxB protein (58,611 Da) exhibits a similarity to bovine 5'-nucleotidase (50). SoxCD is an ₂₂ heterotetramer, with SoxC (43,442 Da) as the molybdenum cofactor-containing subunit with a significant similarity to sulfite oxidases. The diheme c-type cytochrome SoxD (37,637 Da) is tightly associated with SoxC (32). SoxCD increases the yield of electrons in the reconstituted thiosulfate-oxidizing system (18), and SoxC is essential for lithotrophic growth with thiosulfate (50).

soxE was predicted to encode a periplasmic diheme c-type cytochrome (23,616 Da) which was thought to be associated with SoxF to form flavocytochrome c (50). The partial soxF gene predicted a periplasmic polypeptide with high identity to FccB, a flavoprotein of the phototrophic purple sulfur bacterium Allochromatium vinosum which is associated with FccA, the cytochrome subunit of flavocytochrome c. FccAB catalyzes hydrogen sulfide oxidation in vitro (51). Disruption of fccA did not affect phototrophic growth of A. vinosum with hydrogen sulfide. Therefore, sulfide-quinone reductase (SQR) was considered essential for growth of A. vinosum with hydrogen sulfide (35). Inactivation of SQR demonstrates its essential role for phototrophic growth of Rhodobacter capsulatus with hydrogen sulfide (41). SoxF is not required for thiosulfate-dependent cytochrome c reduction of P. pantotrophus in vitro. Also, SoxCD, a sulfite dehydrogenase homologue, is not essential for this reaction (17). P. pantotrophus forms a membrane-bound SQR (40). The presence of SQR, the identification of SoxF as a possible subunit of flavocytochrome c-sulfide dehydrogenase, and the identification of the Sox system posed the question of which system was essential for hydrogen sulfide oxidation in this strain (40). Hydrogen sulfide dehydrogenase activity has been assigned to SoxB (39); however, the exclusive involvement of SoxB in hydrogen sulfide oxidation has been questioned (17), which prompted us to reexamine hydrogen sulfide oxidation in this strain.

In this study we report the cloning of novel genes soxFGH. We describe the thiosulfate-induced formation of SoxF, SoxG, and SoxH and characterize the purified proteins. We demonstrate that the Sox system of P. pantotrophus oxidizes different reduced inorganic sulfur compounds. The SoxFGH proteins are not required for sulfur oxidation in vitro and in vivo as evident from biochemical studies, from a mutant with a deletion in soxF, and from complementation analysis.

	MATERIALS AND METHODS

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Bacterial strains and plasmids. The strains and plasmids used and constructed in this study are listed in Table 1.

Media and growth conditions. P. pantotrophus GB17 was cultivated aerobically at 30°C. Lithoautotrophically grown cells were cultivated in mineral medium (pH 8.0) with 20 mM thiosulfate (10). Inocula were cultivated mixotrophically in the same mineral medium with 20 mM thiosulfate and 20 mM disodium succinate. Lithotrophic mass cultivation (32) of P. pantotrophus GBsoxF was performed in a 300-liter fermentor with a 220-liter working volume (Bioengineering, Wald, Switzerland). With formate or molecular hydrogen as an energy source, cells were cultivated as previously described (10).

Plasmid transformation, conjugation, and gene replacement. E. coli was transformed either as described previously (11) or with the aid of RbCl-treated competent E. coli XL1-Blue cells (37). E. coli S17-1 was used to mobilize plasmids into P. pantotrophus GB17 (43). S17-1 was transformed with plasmid pRD123.2. Donor and recipient were cultivated in nutrient broth (Difco). Aliquots of the mating suspensions (10⁹ cells per ml, which contained an initial donor-recipient ratio of 1:1) were plated on nutrient broth plates and incubated at 37°C for 18 h. Cells were scraped off the plates with 36 mM phosphate buffer, pH 7.2. Kanamycin-resistant cointegrates were selected on mineral agar plates containing 10 mM glucose and 300 µg of kanamycin/ml. Cointegrates were resolved after a period of nonselective growth over several days in 10 ml of mineral medium (pH 8.0) with 20 mM succinate. From strain GB17::pDR123.2, kanamycin-sensitive colonies were further analyzed for double-recombinant strains.

DNA techniques. Standard DNA techniques were applied (37). Plasmids were prepared in small scale from E. coli as described by Kieser (22). For DNA sequencing, plasmid DNA was prepared with a High Pure plasmid isolation kit (Boehringer, Mannheim, Germany). Genomic DNA was isolated according to the protocol of Ausubel et al. (4) Restriction enzymes, T4 DNA ligase, alkaline phosphatase, and Klenow polymerase were obtained from Gibco BRL or Boehringer and used as recommended by the manufacturer. Agarose gel electrophoresis was done in Tris-borate-EDTA buffer (37). DNA fragments were eluted from agarose gels using either the QIAquick gel extraction kit (Qiagen, Düsseldorf, Germany) or an electroelution system. Colony and Southern hybridizations were performed using a digoxigenin (DIG) kit (Boehringer). DNA was sequenced by primer walking with fluorescent primers in an automated DNA sequencer (Li-Cor; MWG-Biotech) by the dideoxy-chain termination method (38) with Taq polymerase and 7-deaza-dGTP from Amersham-Buchler. The DNA sequence of the 5-kb insert of pSOXF5N was analyzed at the facilities of Eurogentec (Seraing, Belgium). Sequence analysis was performed with the BLAST Search Program package (2).

Cloning of soxFGH. Genomic DNA from P. pantotrophus GB17 (50 µg) was dialyzed twice against 2 liters of 200 mM NaCl, precipitated with ethanol, and resuspended in distilled water. Ten micrograms of this DNA was digested with NotI and separated in a 0.8% agarose gel. DNA fragments of about 5 kb were recovered from the gel and cloned into the NotI-restricted vector pBluescript SK(+). A DIG-labeled probe for colony hybridization was generated by PCR using the primers SK2 (5'-CGACCTGCTTCTTCTCG-3') and KS1 (5'-GCATCGCCTCGATCAT-3') and the plasmid pBSK⁺E1 as a template, producing a 315-bp DNA fragment. DNA from positive clones was isolated for further restriction analysis. One of the analyzed clones was designated pSOXF5N, and the 5-kb insert was sequenced.

Construction of a deletion in soxF. A 282-bp fragment was deleted from soxF by PCR technology due to the lack of appropriate restriction sites; the strategy is summarized in Fig. 1. PCRs were performed with Taq polymerase essentially as described elsewhere (27). Using plasmid pEG5 as a template, a fragment of 1,987 bp, designated the left flank, was generated by primer 1 (5'-AAAAGTCGACAGGTCGTGCTGCCCAAT-3'), complementary to bp 8443 to 8459, and primer 2 (5'-TTTTAAGCTTCATGGCGAAGACGCCGC-3'), complementary to bp 10423 to 10407. The right flank was produced by primer 3 (5'-AAAAAAGCTTGTGATCCCGGCCCAGAG-3'), matching bp 10707 to 10722, and primer 4 (5'-TTTTGTCGACTCATCATCGACCATTGC-3'), matching bp 12690 to 12674, with plasmid pSOXF5N as a template, leading to a 1,991-bp fragment. These primers introduced restriction sites for HindIII and SalI into the ends of the fragments. To obtain 5'-protruding ends, the PCR fragments were digested with HindIII and SalI (Fig. 1) and were cloned between the HindIII and SalI sites of pUC19 (45), respectively, which resulted in pRD119.35 (right flank) and pRD120.1 (left flank). The left and right flanks were reisolated as HindIII-SalI fragments from pRD119.35 and pRD120.1 and inserted in one step into the SalI-treated positive selection vector pJOE773.2 (1), resulting in the plasmid pRD121.1. In this plasmid the two fragments are fused via the generated HindIII site within soxF. The 3,978-bp SalI fragment carrying the deletion in soxF along with its neighboring genomic regions was cloned into the SalI site of the mobilizable suicide vector pJD1 (14), leading to the plasmid pRD123.1, which was conjugated into P. pantotrophus. Homogenote recombinants with the deletion in soxF were detected by amplification of a DNA fragment reduced by 276 bp compared with the wild-type soxF by using primers 5 (5'-AAAATCTAGAATGGCCAGGGATTCCCA-3'), corresponding to bp 10025 to 10041, and 6 (5'-TTTTGTCGACCCAGCCGAGGCTTTCCT-3'), complementary to bp 11113 to 11097. For quick preparation of genomic DNA, 1 ml of an overnight culture (Luria-Bertani medium) was harvested, resuspended in 100 µl of TE buffer, and boiled for 10 min. Ten microliters of the crude extract was subjected to DNA amplification. PCR products were purified with QIAquick (Qiagen) and analyzed by agarose gel electrophoresis.

Identification of the Sox character. To identify the Sox character, single colonies were cultivated in thiosulfate mineral medium (5 ml, pH 7.2) supplemented with 20 mM succinate and 10 µg of phenol red/ml on a roller at 30°C overnight. Thiosulfate oxidation caused a drop in pH that was visible as a color change from red to yellow.

Preparation of cell extracts. Cell extracts were prepared with a French press (16). To eliminate unspecific cytochrome c reductions from the crude extract, the protein was precipitated by 44 to 65% ammonium sulfate saturation. The precipitate was separated by centrifugation, redissolved in 25 mM sodium-potassium phosphate buffer, and designated the cell extract.

Purification of SoxF, SoxG, and SoxH. An extract of cells grown with thiosulfate was subjected to differential centrifugation, ammonium sulfate fractionation, and chromatography on Q Sepharose. Proteins were eluted from the Q Sepharose column with a step gradient of 400 ml each at 0.05, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, and 0.4 M sodium chloride as detailed previously (18).

(i) SoxF. SoxF was eluted mainly with 0.1 M sodium chloride and identified by immunoblot analysis as described below. Of this fraction, 70 ml was concentrated to 15 ml by ultrafiltration using an Amicon PM10 membrane. To the concentrate 30 ml of 25 mM sodium potassium phosphate buffer (pH 6.5) containing stabilizing additives (2 mM sodium thiosulfate, 1 mM magnesium sulfate, and 1 µM phenylmethylsulfonyl fluoride [PMSF]) was added and concentrated to 11 ml. This extract was applied to a 6-ml Resource Q column equilibrated against 10 mM bis-Tris buffer (pH 6.0) containing the stabilizing additives specified above. Protein was eluted with a linear gradient of 0 to 500 mM sodium chloride. SoxF-containing fractions were pooled (11.5 ml) and mixed with 90 ml of 10 mM potassium phosphate buffer containing 2 mM sodium thiosulfate, 1 mM magnesium sulfate, and 1 µM PMSF and concentrated to 20 ml. The extract was applied to a hydroxyapatite column (2.6 by 2.5 cm) equilibrated with the same buffer. SoxF was eluted with equilibration buffer, while contaminating proteins remained bound to the column.

(ii) SoxG. SoxG was identified by immunoblot analysis as described below. SoxG was eluted from the Q Sepharose column at 0.3 M sodium chloride. The pooled fractions (90 ml) were concentrated to 4 ml by Amicon ultrafiltration, and the protein was subjected to gel filtration over a Sephacryl S-200 HR column (1.6 by 60 cm) equilibrated with 25 mM sodium potassium phosphate buffer (pH 6.5) containing 2 mM sodium thiosulfate, 1 mM magnesium sulfate, and 1 µM PMSF. Protein was eluted with the same buffer at a flow rate of 45 ml/h, and fractions of about 1.8 ml were collected. The fractions (1.8 ml) containing SoxG were pooled (9.5 ml) and saturated to 30% with ammonium sulfate. The protein was applied to a phenyl-Sepharose HP column (1.6 by 4 cm) equilibrated against 10 mM potassium phosphate buffer containing 2 mM sodium thiosulfate, 1 mM magnesium sulfate, and 1 µM PMSF that was 30% saturated with ammonium sulfate. Protein was eluted with 240 ml of a linear gradient of 30 to 0% ammonium sulfate at a flow rate of 3 ml/min, and fractions of 4 ml were collected.

(iii) SoxH. SoxH was identified by immunoblot analysis as described below. SoxH was eluted from the Q Sepharose column with 0.35 M sodium chloride. The pooled fractions (100 ml) were concentrated to 4 ml by Amicon ultrafiltration and subjected to gel filtration over a Sephacryl S-200 HR column (1.6 by 60 cm) equilibrated with potassium phosphate as detailed for SoxF. Protein was eluted with the same buffer at a flow rate of 45 ml/h. The fractions (1.8 ml) containing SoxH were pooled (9.5 ml) and brought to 30% ammonium sulfate saturation, and the protein was applied to a phenyl-Sepharose HP column (1.6 by 4 cm) equilibrated against 25 mM sodium potassium phosphate buffer containing 2 mM sodium thiosulfate, 1 mM magnesium sulfate, and 1 µM PMSF that was 30% saturated with ammonium sulfate. Protein was eluted with 240 ml of a linear gradient of 30 to 0% ammonium sulfate at a flow rate of 3 ml/min, and fractions of 4 ml were collected.

Purification of the sulfur-oxidizing enzyme system. SoxXA, SoxYZ, SoxB, and SoxCD, which are required to reconstitute thiosulfate-dependent cytochrome c reduction, were purified to homogeneity from cell extracts of thiosulfate-grown cells as described previously (18).

Enzyme assays. The rate of thiosulfate oxidation from whole cells was determined with an oxygen electrode (Rank Brothers, Bottisham, England). The assay mixture (3 ml) contained 50 µl of cell suspension (optical density at 436 nm = 30) and 100 µmol of K-Na phosphate buffer (pH 8.0). Reactions were started with 30 µl of 0.2 M sodium thiosulfate or 30 µl of 10 M sodium sulfide.

Analytical procedures. Flavin adenine dinucleotide (FAD) was quantified by spectrophotometric analysis at 450 nm using an extinction coefficient of 11.3 cm²/µmol.

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

Cloning of soxFGH NotI-restricted genomic DNA of P. pantotrophus hybridized with the 5' region of soxF using a DIG-labeled probe of the known partial soxF sequence. The signal indicated a fragment with a size of about 5 kb (data not shown). The nucleotide sequence completed soxF and uncovered two additional open reading frames, soxG and soxH.

soxF The soxF gene extended from bp 9884 to 11146. The intervening sequence contained a short inverted repeat with the potential to form a hairpin structure and a ribosome binding site 6 nucleotides 5' of the start codon (50). soxF was predicted to encode a polypeptide of 420 amino acids with a molecular mass of 44,614 Da. A putative signal peptide of 26 amino acids and 2,611 Da exhibited a twin-arginine motif diagnostic for periplasmic proteins which are transported to the periplasm via the Tat system (5, 7, 48). For the mature protein an isoelectric point (pI) of 5.15 was calculated. The deduced amino acid sequence of SoxF was 37% identical and 50% similar to that of the flavoprotein FccB of flavocytochrome c of A. vinosum and exhibited a flavin binding site at cysteine-70 as suggested for FccB (15). Hydrogen sulfide-dependent cytochrome c reduction, designated sulfide dehydrogenase activity, has been demonstrated for FccAB (19). Also, the primary sequence of SoxF is 25% identical and 44% similar to that of DHSU, the predicted flavoprotein of flavocytochrome c sulfide dehydrogenase of Aquifex aeolicus (13).

soxG The soxG gene was located 17 bp downstream of soxF and extended from bp 11164 to 12075, with a putative ribosome binding site 5 bp upstream of the start codon. soxG was predicted to encode a protein of 302 amino acids and 32,401 Da, including a putative signal peptide of 25 amino acids and 2,744 Da with a twin-arginine motif (SRRHFLA) which is indicative of periplasmic proteins with redox centers. From the mature protein, a pI of 4.34 and two zinc binding motifs (THGHPDH and TPGHTPGH) were predicted (28). The protein was partially identical to hypothetical proteins from Streptomyces coelicolor A3 (34) and to beta-lactamase-like proteins from Mycobacterium strains (National Center for Biotechnology Information accession numbers AAD31327 and AAD38170).

soxH The soxH gene extended from bp 12075 to 13028, and the start codon overlapped with the stop codon of soxG. A putative ribosome binding site is located 5 bp upstream of the start codon. From soxH a polypeptide of 317 amino acids and 34,401 Da was deduced, with a leader peptide of 20 amino acids (2,084 Da) containing a double arginine and a pI of 4.56 for the mature protein. The deduced SoxH sequence exhibited identities to a putative beta-lactamase precursor from A. aeolicus (13) and cyclases from different Streptomyces strains (26%) (6, 9) and suggested binding sites for heavy metals (CxxC) and zinc (PGHGHPT).

Sequence analysis of the sox gene region. Reexamination of the sox gene region (18) corrected the former ORF1 as a putative ArsR transcriptional regulator of 149 amino acids (16,470 Da) and uncovered a new ORF2 predicted to encode a periplasmic thioredoxin of 130 amino acids with a putative signal peptide of 26 amino acids and a molecular mass of 11,077 Da for the mature protein, with a predicted pI of 4.29 (Fig. 1). Both genes are also present in Rhodopseudomonas palustris and are part of the sox gene cluster of this strain. They are 47 and 41% identical to those of P. pantotrophus (IGwit database accession numbers RRPA00685 and RRPA00708). Also, both genes are directed inversely to the sox genes in P. pantotrophus and R. palustris.

Induction of SoxE, SoxF, SoxG, and SoxH. SoxE, SoxF, SoxG, and SoxH were specifically detected in cell extracts by immunoblot analysis as specified in Materials and Methods. After SDS-PAGE of extracts from thiosulfate-grown cells, OP-E antibodies detected a 24-kDa antigen (data not shown) whose size matched the predicted size of the mature soxE gene product (23,616 Da). OP-F antibodies determined an antigen of 42 kDa similar to the deduced mature SoxF (42,003 Da). OP-G and OP-H antibodies detected antigens of 30 and 32 kDa, respectively (Fig. 2), similar to those deduced for the mature SoxG and SoxH (31,657 and 32,317 Da). These antigens were detected neither in cells grown lithoautotrophically with molecular hydrogen or autotrophically with formate (Fig. 2) nor in cells grown heterotrophically with glucose (data not shown).

View larger version (62K): [in this window] [in a new window]   FIG. 2.   Western blot analysis of cell extracts from P. pantotrophus GB17 and GBsoxF. Proteins of cell extracts (10 µg of protein per well) were separated by SDS-PAGE. Antibodies raised against OP-C, OP-F, OP-G, and OP-H were used. The wild-type GB17 was cultivated with thiosulfate (lanes 1), with molecular hydrogen (lanes 3), or with formate (lanes 4); GBsoxF was cultivated with thiosulfate (lanes 2).

View larger version (27K): [in this window] [in a new window]   FIG. 3.   Thiosulfate-induced formation of SoxB and SoxG. P. pantotrophus was cultivated in mineral medium (400 ml) with 10 mM sodium succinate plus 10 mM glucose in a 1-liter baffled Erlenmeyer flask. After the cells reached the stationary phase at an optical density at 436 nm of 6.35, sodium thiosulfate (20 mM) was added. Cells (0.7 ml) were collected by centrifugation, resuspended with 150 µl of SDS sample buffer, and incubated at 60 and 100°C for 5 min each. Cell debris was removed by centrifugation, and the supernatant was diluted with four aliquots of sample buffer. From this preparation, 20 µg of protein was subjected to SDS-PAGE. Immunoblotting was performed as described in Materials and Methods. Signal intensity was quantified using the Scion Image software. , oxygen uptake rate; , pH; , SoxG; , SoxB.

SoxF. To identify the soxF gene product, SoxF was subjected to amino acid sequence analysis. The N terminus of SoxF was blocked, and the chemical nature of this block could not be resolved. After tryptic digestion of SoxF, the amino acid sequences of two peptides (DFESLQHGYDK and LVLSPGJEFKPDSVPGXSLE) identified SoxF as the soxF gene product. The molecular mass of SoxF as determined by SDS-PAGE was 42 kDa (Fig. 4A), identical to that deduced from the nucleotide sequence of the mature protein of 42,003 Da. Size exclusion chromatography by native gradient PAGE revealed a mass of 45,000 Da, indicating that SoxF was monomeric (Fig. 4B). Determination of the molecular mass by electrospray ionization mass spectroscopy revealed two masses of 42,832 and 42,481 Da, indicating the presence of a prosthetic group (data not shown). Spectrophotometric analysis of SoxF (1.04 mg/ml) showed two peaks of absorption at 450 and 460 nm, characteristic of FAD (_{450 nm} = 11.3 cm²/µmol), and the absorbance at 450 nm of 0.062 revealed 0.71 mol of FAD per mol of SoxF. No metals were detected in SoxF. Also, SoxF was not associated with a cytochrome, while flavocytochromes c from Chlorobium limicola (42), A. vinosum (51), and Thiobacillus strain W5 (46) are associated with a cytochrome c.

View larger version (19K): [in this window] [in a new window]   FIG. 4.   Determination of molecular masses of SoxF, SoxG, and SoxH by native gradient PAGE (A) and denaturing SDS-PAGE (B) using 1.5 µg of each protein. Lanes: 1, SoxF; 2, SoxG; 3, SoxH.

SoxG. SoxG was verified as the soxG gene product by analysis of the N terminus (ATTVAMGPVRIDRLXDGHLE), whose amino acid sequence is identical to that deduced from soxG, also demonstrating the specificity of the immunochemical detection (Fig. 2). SoxG appeared as a single band of 31 kDa after SDS-PAGE (Fig. 4B). The molecular mass determined by native gradient PAGE was 64 kDa (Fig. 4A), indicating that SoxG was homodimeric. Molecular mass determination by electrospray ionization mass spectroscopy revealed a single mass of 29,656 Da, which was identical to that deduced from the nucleotide sequence of the mature SoxG of 29,657 Da (data not shown). In the SoxG preparation 0.90 mol of zinc, 0.13 mol of copper, and 0.13 mol of iron per mol of SoxG were present, while only trace amounts of other metals were detected (data not shown).

SoxH. SoxH was identified as the soxH gene product by the N-terminal amino acid sequence (SEDIPDQYPQSVLYS), which was identical to that deduced from soxH. Purified SoxH appeared as single bands of 31 kDa in SDS-PAGE (Fig. 4B) and of 54 kDa in native gradient PAGE (Fig. 4A), indicating its homodimeric structure. SoxH as isolated contained 0.28 mol of zinc, 0.20 mol of copper, and 0.11 mol of cobalt per mol of subunit. Other metals were present in negligible amounts (data not shown). The molecular mass of 32,315 Da as determined by electrospray ionization mass spectroscopy was almost identical to that deduced from the nucleotide sequence for the mature SoxH (32,317 Da) (data not shown).

Sulfur-dependent cytochrome c reduction. The specific rate of hydrogen sulfide-dependent horse heart cytochrome c reduction was 0.018 U/mg of protein in cell extracts of thiosulfate-grown P. pantotrophus (data not shown). This activity was reconstituted from fractions of proteins of the cell extract eluted from Q Sepharose at 0.05, 0.25, 0.30, and 0.35 M sodium chloride (data not shown). The pure Sox proteins SoxXA, SoxYZ, SoxB, and SoxCD mediated thiosulfate- and sulfite-dependent cytochrome c reduction (18). This system also mediated hydrogen sulfide- and sulfur-dependent cytochrome c reduction (Table 2). The ratio of activities with hydrogen sulfide, sulfur, thiosulfate, and sulfite as electron donors was 1:0.63:0.39:0.17. Residual sulfur- and hydrogen sulfide-dependent activities of about 3 and 11% were observed when either SoxB or SoxYZ was omitted from the assay (Table 2). Thus, the Sox system of P. pantotrophus represents a new type of hydrogen sulfide dehydrogenase. Moreover, since it oxidized different reduced inorganic sulfur compounds, it was considered to be a general sulfur-oxidizing system.

Construction of GBsoxF. SoxF was not required for the oxidation of reduced sulfur compounds in vitro (Table 2). Therefore, a deletion in soxF was designed to examine its function in vivo. Within soxF, bp 10424 to 10705, coding for 94 amino acids including three cysteine residues, were deleted by PCR technology. The deletion was verified by the reduction of the size of the mutant PCR fragment. A 1,109-bp fragment was generated from the wild type, and an 833-bp fragment was generated from the mutated genome of GBsoxF (Fig. 5). The construction of the deletion generated a HindIII restriction site by combining the left and right genomic flanks, and two additional amino acids, Lys and Leu, were created in the altered SoxF. The correct construction was demonstrated from the HindIII restriction site within the 833-bp PCR fragment amplified from mutant DNA (data not shown).

View larger version (40K): [in this window] [in a new window]   FIG. 5.   PCR analysis of the deletion in soxF of GBsoxF. Genomic DNA was used as a template for PCR with primers 5 and 6 binding within soxF. Purified PCR assay mixtures (4 µl) were applied to each well. Lanes: 1, HindIII-digested  DNA; 2, plasmid control pRD123.2; 3, mutant GBsoxF; 4, cointegrate GB17::pRD123.2; 5, GB17.

Characterization of GBsoxF. Cell extracts from autotrophically grown P. pantotrophus and the mutant GBsoxF were analyzed for SoxF antigens using Western blot analysis. With antibodies against OP-F, a band of 42 kDa was detected for GB17 (Fig. 2), as for pure SoxF (Fig. 4A). As a result of the deletion no signal was detected in extracts of GBsoxF (Fig. 2), as the immunogenic epitope was located within the deleted middle part of the SoxF protein (32.2 kDa). Strain GBsoxF grew lithotrophically with thiosulfate, and no significant differences were detected with respect to specific growth rate, cellular yield, and rates of oxidation of hydrogen sulfide and thiosulfate compared to the wild type (data not shown).

Complementation of the Sox system. Using OP-B and OP-C antibodies, the respective antigens were not detected in extracts of the Tn5 insertional mutant strain TP19 when cultivated mixotrophically with succinate and thiosulfate (data not shown). Thus, a functional Sox system could not be reconstituted. Therefore, the structural genes required for sulfur oxidation in vitro were complemented with pVKB9, which does not contain the soxFGH genes (Fig. 1). P. pantotrophus TP19(pVKB9) grew lithotrophically with thiosulfate at a rate and extent similar to those of the wild type, and SoxB and SoxC antigens were detected by Western blot analysis in this strain. SoxG antigens were not detected in strain TP19(pVKB9) (data not shown). This result indicated that the soxFGH gene products were not essential for lithotrophic growth with thiosulfate.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

In the present study the sequence of the sox gene cluster of P. pantotrophus was completed, and three genes, soxFGH, and their products were characterized. The respective proteins were predicted to be located in the periplasm, and their formation was induced by thiosulfate. However, the soxFGH gene products were not required for growth with thiosulfate. Homogenous SoxF, SoxG, and SoxH did not exhibit sulfur-oxidizing activity and were not required for sulfur oxidation in vitro. Instead, the Sox system reconstituted from SoxXA, SoxYZ, SoxB, and SoxCD was found to generally oxidize inorganic reduced sulfur compounds, namely, thiosulfate, sulfite, sulfur, and hydrogen sulfide.

SoxF is a flavoprotein, as evident from the identification of FAD. The molecular mass (42 kDa) of SoxF of P. pantotrophus was similar to those of flavoproteins (40 to 47 kDa) from other sources (17). SoxF was not associated with a c-type cytochrome as described for flavocytochromes c of different strains of the genus Chlorobium, of A. vinosum, and of Thiobacillus strain W5, which exhibit sulfide dehydrogenase activity in vitro (46, 51). No catalytic activity was detected for SoxF alone or in combination with a Q Sepharose fraction containing SoxE. However, SoxF inhibited hydrogen sulfide oxidation significantly, by about 20%. This mode of action is so far unexplained. Also, a deletion within soxF and complementation analysis did not indicate a function for growth with thiosulfate. No catalytic activity was detected for the homodimeric zinc-containing protein SoxG and the homodimeric SoxH protein, which possibly contains zinc and copper. No possible function could be deduced from alignments of SoxG and SoxH; thus, their specific functions are presently unknown.

SoxF has high identity to the flavoprotein FccB of flavocytochrome c sulfide dehydrogenase of A. vinosum. Flavocytochrome c of A. vinosum also binds sulfite, thiosulfate, and mercaptans, and its function in vivo as a sulfide dehydrogenase has been questioned (12, 15). There is no experimental support for a crucial role of flavocytochrome c in sulfur metabolism, although the ability to grow with thiosulfate is seemingly correlated with its presence in different sulfur-oxidizing bacteria (17). Disruption of the gene for the cytochrome subunit of flavocytochrome c of A. vinosum did not affect phototrophic growth with hydrogen sulfide, and SQR was suggested to be responsible for this metabolism (35). In fact, deletion of the sqr gene of R. capsulatus demonstrated that SQR is essential for phototrophic growth with hydrogen sulfide (41), the only sulfur compound that this strain is able to utilize for this metabolism. SQR is also present in membranes of P. pantotrophus, and its physiological function in this strain is not known (40).

The reconstituted sulfur-oxidizing enzyme system of P. pantotrophus performs sulfite-, thiosulfate-, sulfur-, and hydrogen sulfide-dependent cytochrome c reduction. The highest activity was observed with hydrogen sulfide. This substrate versatility characterizes the enzyme complex as the first to generally oxidize reduced inorganic sulfur compounds and corrects a previous report suggesting SoxB to act as a sulfide dehydrogenase (39). The biochemical data are supported by previous reports of a soxB::Tn5 insertional mutant unable to oxidize thiosulfate and hydrogen sulfide (10, 49).

Using the reconstituted Sox system, 1 mol of thiosulfate yielded about 8 mol of electrons, which was in accordance with the theoretical yield. However, with hydrogen sulfide or sulfur as substrate, a yield of only about 4 mol of electrons per mol of substrate was observed. Autoxidation of hydrogen sulfide was excluded by anaerobic performance of the assays. Thus, the low electron yield is presently unexplained. The material balance of sulfur substrates and products is hampered by the interference of proteins present in the assays for quantitative analysis. Omission of SoxCD from assays with sulfur or hydrogen sulfide as an electron donor resulted in a yield of 2 mol of electrons, which was in accordance with a previous finding with sulfite and thiosulfate as substrates (18) and supported the finding that anaerobic conditions did not alter the yield of electrons. The enhancement of the yield of electrons by SoxCD points to a function different from sulfite dehydrogenase and suggests for SoxCD the novel function of a sulfur dehydrogenase.

The Sox system described for P. pantotrophus appears to be also present in polythionate-oxidizing sulfur-oxidizing bacteria. Using the system for insertional mutagenesis previously applied for P. pantotrophus (10), a soxA gene was inactivated in the polythionate-utilizing facultatively lithotrophic thiobacterium KCT001, resulting in a Sox phenotype (30). Analysis of the partial nucleotide sequence of strain KCT001 indicated the presence of a soxZ'AB' gene sequence. The deduced partial SoxZ is 64% identical in a 33-amino-acid overlap to SoxZ of P. pantotrophus, the diheme SoxA is 45% identical, and the twin-arginine motif of the putative leader peptide of SoxB is identified. The order of sox genes in strain KCT001 suggested a gene cluster similar to that of P. pantotrophus. Also, the order of the soxB'CD' genes has been detected in the thiosulfate- and tetrathionate-oxidizing bacterium Starkeya novella (20). Moreover, genes homologous to the sox genes of P. pantotrophus have also been detected in phototrophic bacteria like R. palustris (IGwit database accession number RRPA00660) and Chlorobium tepidum (IGwit database accession number RCL01710). Thus, the sox gene cluster of P. pantotrophus may represent a general Sox system also present in different sulfur-oxidizing bacteria, including polythionate-utilizing strains. Polythionate hydrolysis yields sulfate and sulfane monosulfonic acids. These compounds or spontaneous decomposition products may be further metabolized via such a common sulfur-oxidizing system. In light of the recent genetic data (18, 30) and the biochemical data reported here, the distinction between polythionate-utilizing and non-polythionate-utilizing sulfur-oxidizing bacteria (17, 21) is not applicable with respect to the system that actually oxidizes sulfur.