INTRODUCTION

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The bacterium Deinococcus radiodurans is extremely resistant to many lethal and mutagenic agents and conditions that damage DNA, including ionizing radiation, UV radiation, and hydrogen peroxide treatment (35). D. radiodurans is one of the most radiation-resistant organisms; exponentially growing cells are 200 times more resistant to ionizing radiation and 20 times more resistant to UV irradiation than Escherichia coli (2, 3). It has been suggested that this elevated resistance of D. radiodurans is due to unusually efficient DNA repair and recombination mechanisms (3, 36), but the molecular mechanisms responsible for radiation resistance remain unknown. Recently, many putative genes involved in DNA repair and recombination have been identified in the genome of D. radiodurans (27, 50). Information regarding the functions of these repair enzymes is emerging.

To protect their genomes from the oxidative DNA damage caused by ionizing radiation, cells have evolved efficient and accurate repair systems to remove DNA lesions (12). 7,8-Dihydro-8-oxoguanine (GO) is the most stable product known to be caused by oxidative damage to DNA. If not repaired, GO lesions in DNA can produce A/GO mismatches during DNA replication (43) and can result in transversions from G · C to T · A (9, 37, 38, 53). In E. coli, three enzymes, MutY, MutM (Fpg), and MutT, are known to be involved in defending against the mutagenic effects of GO lesions (32, 45). MutT hydrolyzes oxidized dGTP and depletes it from the nucleotide pool (28). The function of the MutM (Fpg) protein is to remove the mutagenic GO and other oxidized purines (46). MutY protein is responsible for correcting A/GO mismatches, as well as A/G and A/C mismatches (1, 23, 48). Thus, MutY provides a measure of defense by removing adenines misincorporated opposite GO or G following DNA replication (25, 31, 32). Homologs of putative MutY, MutM, and MutT are present in D. radiodurans (27, 50).

The E. coli MutY (MutY_Ec) protein is a 39-kDa iron-sulfur protein (33, 48, 49), and its N-terminal domain exhibits structural similarity with those of endonuclease III (endo III) and AlkA (6, 16, 33, 39, 48, 49). This includes the helix-hairpin-helix (HhH) and Gly/Pro...Asp loop motifs. The conserved Asp acts as a general base to activate a nucleophile, such as Lys or water. DNA glycosylases in the endo III superfamily can be divided into two groups (11, 13). Bifunctional DNA glycosylases, including endo III and human 8-oxoG glycosylase (hOGG1), use the conserved lysine (Lys120 in endo III and Lys249 in hOGG1) to form a Schiff base intermediate and also possess strong apurinic/apyrimidinic (AP) lyase activity, which cleaves DNA 3' to an AP site by a -elimination mechanism (14, 39, 40, 47). Monofunctional glycosylases, such as AlkA, lack both the conserved lysine and AP lyase activity (17, 44, 56). The possession of AP lyase activity by MutY is controversial. MutY AP lyase activity has been detected by some researchers (15, 22, 25, 26, 29, 30, 48) but not by other groups (1, 7, 31, 34, 44). Although MutY lacks the conserved lysine, there is evidence that MutY may be a bifunctional glycosylase. MutY can cleave DNA containing an unmodified AP site (30, 54). The enzyme also forms a covalent complex with its DNA substrates in the presence of sodium borohydride (15, 26, 30, 51, 57), a diagnostic tool for bifunctional glycosylase/AP lyase (13, 40, 44). Lys142 of MutY_Ec has been shown to form the Schiff base intermediate with DNA (52, 54, 57); however, K142A mutant MutY has glycosylase activity (52, 54) and can promote / elimination with AP-containing DNA (54). This raises the question of whether Schiff base formation is significant for the MutY function.

MutY_Ec has an additional C-terminal domain that has no counterpart in other members of the HhH family of proteins. Although the N-terminal domain of MutY_Ec exhibits catalytic activity (15, 29, 30, 41), the C-terminal domain has been shown to play an important role in recognition of GO lesions (15, 20, 41). The truncated MutY has >18-fold-lower binding affinities with GO-containing mismatches than the intact MutY has (20). Deletion of the C-terminal domain of MutY reduces its catalytic preference for A/GO-containing DNA over A/G-containing DNA (10, 20, 41) and confers a mutator phenotype in vivo (10, 20). Moreover, MutY uses its C-terminal domain to attenuate the catalytic activity of MutM (Fpg) protein (20). These findings strongly support the notion that the C-terminal domain of MutY plays an important role in GO recognition and mutation avoidance.

Because D. radiodurans is extremely resistant to oxidative stress, we tried to characterize and compare its MutY homolog (MutY_Dr) with MutY_Ec. Like MutY_Ec, MutY_Dr does not have the conserved lysine downstream of the HhH motif. In contrast to MutY_Ec, an Arg is at the position that corresponds to Lys142 of MutY_Ec. Our results indicate that MutY_Dr is a monofunctional adenine glycosylase. The substrate specificities and kinetic parameters of MutY_Dr are similar to those of MutY_Ec. MutY_Dr that is expressed is able to complement E. coli mutY mutants but not mutM mutants to reduce the mutation frequency. Although MutY_Dr can protect D. radiodurans from GO mutational effects, we identified no specific properties of MutY_Dr that were drastically different from MutY_Ec properties that could contribute to the high levels of resistance to ionizing radiation and oxidative stress of this organism.

	MATERIALS AND METHODS

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Bacteria and DNA. The genomic DNA of D. radiodurans R1 was obtained from M. J. Bessman. E. coli PR8 (Su lacZ X74 galK Sm^r) and PR70 (like PR8 but micA68::modified Tn10) were obtained from M. S. Fox. E. coli CC104 [ara (gpt-lac)5 F'(lacI378 lacZ461 proA⁺B⁺)] and CC104/mutYmutM (like CC104 but mutY::mini-Tn10 mutM) were obtained from J. H. Miller. E. coli MV1161 [thr-1 ara-14 leuB6 (gpt-proA)62 lacY1 tsx-33 supE33 galK2 hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 rfa-550] and MV3867 (like MV1161 but mutM472::Tn10) were obtained from M. Volkert. E. coli Tuner(DE3) [F ompT hsdS_B(r_B m_B) gal dcm lacY1 (DE3)] and DH5 [supE44 lacU169 (80 lacZ M15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1] were obtained from Novagen and Life Technologies, Inc., respectively. Cells harboring DE3 lysogen were constructed by using the procedures described by Invitrogen.

Cloning of MutY_Dr. According to the previously published genomic sequence of D. radiodurans R1 (accession no. NC001263) (50), the putative mutY homolog sequence (DR2285) contains 1,089 base pairs and codes for a 363-residue protein. To clone the MutY_Dr gene, we synthesized the following set of PCR primers: forward strand, 5'-GCGCGCTCATGACGTTGCCCGTGTCTGCC-3'; and reverse strand, 5'-GGCGCGCCTTACGCCTCGCCCAGCGGGGAACTG-3'. Genomic DNA prepared from D. radiodurans R1 was used as a template for PCRs. The PCR mixtures (100 µl) contained 20 ng of chromosomal DNA, 100 pmol of each primer, 1.5 mM MgCl₂, and 5 U of Pfu DNA polymerase (Stratagene). The reactions were performed as follows: 95°C for 2.0 min, 60°C for 1.0 min, 72°C for 2.0 min for 30 cycles, and a final extension step at 72°C for 10 min. The 1.1-kb PCR product was purified with a QIAquick PCR purification kit (QIAGEN), digested with BspHI and AscI, ligated into NcoI- and AscI-digested pETBlue-2 vector (Novagen), and transformed into DH5 cells. Sequence analysis of a selected clone (pETBlue-2DR2285) (GenBank accession no. AF377342) revealed that its DNA sequence is exactly the same as the sequence predicted for MutY_Dr (accession no. NC001263).

Measurement of mutation frequency. Plasmid pETBlue-2DR2285 was transferred to E. coli PR70/DE3 (mutY), CC104/DE3/mutYmutM, and MV3867/DE3 (mutM) cells to check their mutation frequencies. Independent overnight cultures were grown to an A₅₉₀ of 0.7 in Luria-Bertani (LB) medium containing 50 mg of ampicillin per ml when necessary. After 2.5 h of induction by 0.1 mM isopropyl-1-thio--D-galactopyranoside, 0.1 ml of cells from each culture was plated onto LB agar containing 0.1 mg of rifampin per ml. The cell titer of each culture was determined by plating a 10⁶ dilution onto LB agar. The ratio of Rif^r cells to total cells was the mutation frequency.

Overexpression and purification of MutY_Dr. Plasmid pETBlue-2DR2285 was transferred into Tuner(DE3) cells to express the 39.4-kDa MutY_Dr protein. Six liters of a MutY_Dr-overproducing strain was grown to an A₅₉₀ of 0.7 in LB broth containing 50 mg of carbenicillin per ml at 37°C. The cells were induced by adding isopropyl-1-thio--D-galactopyranoside to a concentration of 0.8 mM, cultured overnight at 20°C, and harvested by centrifugation.

MutY_Dr binding assay. Binding of MutY_Dr to various oligonucleotides was assayed by a gel retardation procedure similar to a procedure described previously (21). ³²P-labeled 20-bp oligonucleotides (1.8 fmol) were incubated with MutY_Dr in 20 µl of binding buffer containing 10 mM Tris-HCl (pH 7.6), 0.5 mM dithiothreitol, 20 mM NaCl, 0.1 mM EDTA, 2.9% glycerol, 50 µg of bovine serum albumin per ml, and 5 ng of poly(dI-dC) at 37°C for 30 min. MutY_Dr protein was diluted with a buffer containing 20 mM potassium phosphate (pH 7.4), 1.5 mM dithiothreitol, 0.1 mM EDTA, 50 mM KCl, 200 µg of bovine serum albumin per ml, and 50% glycerol. Protein-DNA complexes were analyzed on 8% polyacrylamide gels in 50 mM Tris borate (pH 8.3)-1 mM EDTA. The apparent dissociation constants (K_d values) of MutY_Dr and DNA were determined by using nine MutY_Dr concentrations, and experiments were repeated at least three times. Bands corresponding to enzyme-bound and free DNA were quantified by using PhosphoImager images, and K_d values were obtained from analyses by using a computer-fitted curve generated by the Enzfitter program (19).

MutY_Dr glycosylase assay. Glycosylase assays were carried out like the binding assay except that no poly(dI-dC) was added to 10-µl reaction mixtures. Unless specified otherwise, after incubation at 37°C for 30 min, reaction mixtures were dried with a Speed Vac (Savant), resuspended in 3 µl of formamide dye (90% formamide, 10 mM EDTA, 0.1% xylene cyanol, 0.1% bromophenol blue), heated at 90°C for 2 min, and loaded onto 14% polyacrylamide-7 M urea sequencing gels that were electrophoresed at 2,000 V. To test for AP lyase activity, the glycosylase reactions were performed in 10-µl reaction mixtures. In reaction condition I, the sample was supplemented with 5 µl of formamide dye and directly loaded onto a gel that was electrophoresed at 1,000 V without drying and heating. The sample in reaction condition II was supplemented with 5 µl of formamide dye and heated at 90°C for 2 min before it was loaded onto a gel without drying. The sample in reaction condition III was treated with 1 M piperidine at 90°C for 30 min after the reaction, dried, resuspended in 3 µl of formamide dye, and heated at 90°C for 2 min.

Formation of enzyme-DNA covalent complex. Reactions were carried out as described above for the MutY_Dr glycosylase assay, except that the reactions were performed in the presence of NaBH₄. An NaBH₄ stock solution was freshly prepared immediately prior to use. After incubation at 37°C for 30 min, sodium dodecyl sulfate (SDS) dye (final concentrations, 30 mM Tris-HCl [pH 6.9], 5% [vol/vol] glycerol, 1% [wt/vol] SDS, 1% [vol/vol] -mercaptoethanol, and 0.1 mg of bromophenol blue per ml) was added to the samples, which were heated at 90°C for 2 min and separated on a 12% polyacrylamide gel in the presence of SDS as described by Laemmli (18), and the gel was dried and exposed to a PhosphoImager screen.

	RESULTS

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Analysis of the MutY_Dr protein sequence. Because D. radiodurans is extremely resistant to oxidative stress, we tried to clone the mutY gene homolog(s) from the sequenced genome (50). A Blast search identified four open reading frames that exhibit high levels of homology with MutY_Ec: DR2285, DR0289, DR2438, and DR0928. Only DR2285 contains the extra C-terminal domain like MutY_Ec. Because the C-terminal domain of MutY_Ec has no counterpart in other proteins belonging to the HhH family, the presence of this domain in DR2285 suggests that DR2285 is a putative MutY homolog. DR2285 (designated MutY_Dr) contains 363 amino acid residues and exhibits 35% identity to MutY_Ec, as determined by an ALIGN program (Fig. 1). Specifically, two regions with the highest levels of similarity were found; residues 127 to 132 of MutY_Dr are located at the conserved HhH motif, and residues 189 to 219 of MutY_Dr contain the iron-sulfur domain with four conserved Cys residues (16).

View larger version (61K): [in this window] [in a new window]   FIG. 1.   Comparison of amino acid sequences of MutYDr and MutYEc. Identical and conserved residues are indicated by black and gray boxes, respectively. The sequences used were the sequences of E. coli MutY (EcMutY) (accession no. P17802) and D. radiodurans MutY (DrMutY) (accession no. NC 001263). The conserved Asp residue is indicated by a dot. The position of Ser120 of MutYEc and Tyr134 of MutYDr at the conserved Lys downstream of the HhH motif of the HhH family is indicated by an asterisk. A triangle indicates the position of Lys142 of MutYEc and Arg156 of MutYDr.

Reduction of the mutation frequency of an E. coli mutY mutant by MutY_Dr. To demonstrate that the putative MutY_Dr protein is a functional homolog of MutY_Ec in vivo, we measured the mutation frequencies of MutY_Dr-expressing E. coli strains. The mutY_Dr gene under control of the T7 promoter in the pETBlue-2 vector was expressed in three E. coli strains, PR70/DE3 (mutY), MV3867/DE3 (mutM), and CC104/DE3/mutYmutM (mutY mutM double mutant). As shown in Table 1, the mutY mutant (PR70/DE3) exhibited a 46-fold-higher mutation frequency than the wild-type PR8 strain. PR70/DE3 cells expressing MutY_Dr had a mutation frequency as low as that of the wild-type cells, while the vector pETBlue-2 alone had a 22-fold-higher mutation frequency than the wild type (Table 1). Table 1 shows that the mutY mutM double mutant (CC104/DE3/mutYmutM) exhibited a mutation frequency that was more than 900-fold higher than that of wild-type strain CC104/DE3. CC104/DE3/mutYmutM cells expressing MutY_Dr could have a mutation rate close to that of the wild type (Table 1). No obvious effect was observed when MutY_Dr was expressed in the mutM mutant strain, MV3867/DE3. Thus, MutY_Dr indeed is a MutY_Ec functional homolog.

Effects of salt and EDTA on the binding and glycosylase activities of MutY_Dr. To further demonstrate that MutY_Dr encodes a functional MutY protein, we purified the recombinant MutY_Dr expressed in E. coli Tuner(DE3) and assayed its activities. The MutY_Dr protein was purified by ammonium sulfate precipitation and phosphocellulose, hydroxylapatite, MonoQ, and MonoS chromatographic steps. We recovered about 19 mg of MutY_Dr protein from 22 g of cell paste. As judged on an SDS-12% polyacrylamide gel, the protein was purified to >99% homogeneity (Fig. 2, lane 9). The mobility of MutY_Dr (the single band in Fig. 2, lane 8) in the denatured gel matched the predicted size (39.4 kDa). The MutY_Dr band in fraction VIIc cross-reacted weakly with polyclonal antibodies against MutY_Ec and the MutY homolog (MYH) of Schizosaccharomyces pombe (data not shown).

View larger version (95K): [in this window] [in a new window]   FIG. 2.   SDS-polyacrylamide gel analysis of MutYDr. The proteins were separated on a 12% polyacrylamide gel in the presence of SDS and stained with Coomassie blue. Protein markers (Gibco BRL prestained protein marker) were run in lane 1. Lanes 2 to 9 contained fractions I (11 µg; crude cell extract), II (11 µg; fraction after streptomycin sulfate treatment), III (7.3 µg; fraction after ammonium sulfate treatment), IV (3 µg; fraction after phosphocellulose column elution), V (1.8 µg; fraction after hydroxylapatite column elution), VI (1.5 µg; fraction after MonoQ column elution), VIIc (1.5 µg; fraction after MonoS column elution), and VIIc (7.5 µg; fraction after MonoS column elution), respectively. Excess protein was loaded in lane 9 to show the degree of homogeneity.

View larger version (53K): [in this window] [in a new window]   FIG. 3.   Effects of EDTA on MutYDr binding and glycosylase activities with A/G- and A/GO-containing DNA. (A) Glycosylase activity. Oligonucleotide 20-mer DNA containing an A/G (lanes 1 to 7) or A/GO (lanes 8 to 14) mismatch was reacted with MutYDr for 30 min at 37°C in the presence of different EDTA concentrations. MutYDr reactions were carried out in buffers in the presence of 1 mM (lanes 1 and 8), 5 mM (lanes 2 and 9), 10 mM (lanes 3 and 10), 20 mM (lanes 4 and 11), 30 mM (lanes 5 and 12), 40 mM (lanes 6 and 13), and 50 mM (lanes 7 and 14) EDTA. The reaction products were analyzed on a 14% polyacrylamide DNA sequencing gel. The arrows indicate the positions of intact oligonucleotide (arrow I) and nicking product (arrow N). (B) DNA binding activity. The EDTA concentrations used were similar to those used in the experiment whose results are shown in panel A. Protein-DNA complexes were analyzed on 8% polyacrylamide gels in 50 mM Tris borate (pH 8.3)-1 mM EDTA. The arrows indicate the positions of protein-bound DNA (arrow B), protein-free double-stranded DNA (arrow F), and single-stranded DNA (arrow S).

MutY_Dr exhibits adenine DNA glycosylase activity and weak guanine DNA glycosylase activity but limited AP lyase activity. The glycosylase activities of MutY_Dr and MutY_Ec with several mismatches were compared. As shown in Fig. 4, MutY_Dr could cleave a 20-mer oligonucleotide containing an A/G, A/GO, or A/C mismatch. An A/C mismatch was a better substrate for MutY_Dr than for MutY_Ec (Fig. 4, compare lanes 5 and 13). MutY_Dr also exhibited weak guanine DNA glycosylase activity with G/GO mismatch-containing DNA, and this activity was weaker than that of MutY_Ec (Fig. 4, compare lanes 8 and 16). In addition, 2-aminopurine/G was also a weak substrate of MutY_Dr (Fig. 4, lane 6). MutY_Dr exhibited no catalytic activity with C/G, C/GO, and T/GO mismatches (Fig. 4, lanes 3, 4, and 7). This substrate specificity is very similar to that of MutY_Ec (Fig. 4, compare lanes 1 to 8 with lanes 9 to 16).

View larger version (66K): [in this window] [in a new window]   FIG. 4.   Glycosylase activities of MutYDr (DrMutY) and MutYEc (EcMutY) with different mismatches. Oligonucleotide substrates (3'-end-labeled 20-mers; 1.8 fmol) containing the mismatches indicated above the lanes were incubated with 3.6 nM MutYDr (lanes 1 to 8) or 3.6 nM MutYEc (lanes 9 to 16) for 30 min at 37°C. Abbreviations: O, GO; 2, 2-aminopurine. The arrows indicate the positions of intact DNA substrate (arrow I) and the cleaved DNA fragment (arrow N). The minor band above the cleaved product was derived from an impurity of DNA substrates that appeared above the intact DNA.

View larger version (51K): [in this window] [in a new window]   FIG. 5.   MutYDr exhibits limited AP lyase activity. (A) Glycosylase reactions performed under different reaction conditions. Lane 1, sample supplemented with 5 µl of formamide dye and directly loaded onto a gel that was electrophoresed at 1,000 V without drying and heating; lane 2, sample supplemented with 5 µl of formamide dye and heated at 90°C for 2 min before it was loaded onto a gel without drying; lane 3, sample treated with 1 M piperidine at 90°C for 30 min after the reaction, dried, resuspended in 3 µl of formamide dye, and heated at 90°C for 2 min; lane 4, DNA alone. The samples were from concurrent experiments, but the products were separated on nonadjacent lanes in one sequencing gel. The arrows indicate the positions of intact oligonucleotide (arrow I), products with a 3'-,-unsaturated aldehyde formed via -elimination (arrow B), and products with a 3' phosphate formed via /-elimination by piperidine (arrow P). (B) Formation of covalent complexes of MutYDr and A/GO-containing DNA in the presence of various concentrations of NaBH4. Lane 1, reaction mixture containing 72 fmol of E. coli MutY in MutYEc buffer (20 mM Tris-HCl [pH 7.6], 1 mM dithiothreitol, 1 mM EDTA, 2.9% glycerol) in the presence of 100 mM NaBH4; lanes 2 to 11, reaction mixtures containing 14.4 fmol of MutYDr with different concentrations of NaBH4 (100, 80, 60, 50, 40, 30, 20, 15, 10, and 5 mM, respectively). The products after heating at 90°C for 2 min were fractionated in an SDS-12% polyacrylamide gel. The positions of free oligonucleotide (arrow F) and a covalent complex (arrow C) are indicated by arrows.

Kinetic parameters of MutY_Dr. The efficiencies of cleavage by MutY_Dr and MutY_Ec of a 20-mer oligonucleotide containing A/G or A/GO were compared (Table 2). As measured at a protein concentration of 0.5 nM, the K_m and V_max values for MutY_Dr with an A/G mismatch were slightly higher than the MutY_Ec values. The turnover number (K_cat) for MutY_Dr with an A/G 20-mer was two times higher than that of MutY_Ec. However, MutY_Dr and MutY_Ec have similar reactivities with both A/G- and A/GO-containing DNA, as measured by specificity constants (K_cat/K_m).

Time course of MutY_Dr activity with A/G- and A/GO-containing DNA. The steady-state kinetics of the MutY_Dr reaction (Table 2), as measured at 37°C for 30 min, cannot reflect the true reactivity because the enzyme may have a low turnover rate. Thus, we used single-turnover glycosylase kinetics to compare the reactivities of MutY_Dr with A/G- and A/GO-containing DNA substrates. Time course studies to determine the extents of glycosylase activity with both A/G- and A/GO-containing substrates were performed. As shown in Fig. 6, the rate of cleavage of MutY_Dr with A/G-containing DNA was lower than the rate of cleavage with A/GO-containing DNA for the first 2 min. The times required to reach 50% of the maximum levels with A/G- and A/GO-containing DNA were 1.28 and 0.14 min, respectively. The difference in the reaction rates was more than ninefold. Figure 6 also shows that at a steady state (>3 min), the MutY_Dr glycosylase activity with a A/GO mismatch was weaker than that with A/G-containing DNA. This result is similar to previous findings for MutY_Ec (20).

View larger version (14K): [in this window] [in a new window]   FIG. 6.   Time course studies of MutYDr glycosylase activities with A/G- and A/GO-containing DNA. A/G-containing () and A/GO-containing () 20-mer oligonucleotides (1.8 fmol) were incubated at 37°C with 72 fmol of MutYDr for various times. After reaction, the products were treated with piperidine, dried, resuspended in formamide dye, heated at 90°C for 2 min, and analyzed on a 14% polyacrylamide denaturing sequencing gel. Data were obtained from PhosphorImager quantitative analyses of gel images from three experiments. The percentages of DNA cleaved were plotted as a function of time.

Binding affinities of MutY_Dr and MutY_Ec with different mismatches. Because MutY_Dr and MutY_Ec have similar glycosylase activities with different substrates (Fig. 4), we determined the apparent dissociation constants (K_d) of MutY_Dr with A/G-, AP/G-, and GO-containing mismatches. As shown in Table 3, MutY_Dr exhibited >100-fold-greater affinity with A/GO-containing DNA than with A/G-containing DNA. MutY_Dr also exhibited affinity with the reaction products AP/G and AP/GO. The affinity of MutY_Dr with the AP/G product was 5.5-fold higher than that with the A/G substrate, while the affinity with the AP/GO product was slightly lower than that with the A/GO substrate. The MutY_Dr binding to a C/GO mismatch was 250-fold lower than that to a A/GO mismatch but only 2-fold lower than that to an A/G mismatch. MutY_Dr exhibited high affinity to a weakly catalytic G/GO substrate and a noncatalytic T/GO substrate (Fig. 4). The K_d values for MutY_Dr are similar to those for MutY_Ec, but the binding affinities of MutY_Dr with all of the substrates tested were slightly lower than those of MutY_Ec.

	DISCUSSION

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In this study, we demonstrated that the DR2285 open reading frame of D. radiodurans encodes a MutY homolog. Expressed MutY_Dr is able to complement the mutator phenotypes of an E. coli mutY mutant and an E. coli mutY mutM double mutant (Table 1). This conservation of the repair function is not surprising as MutY_Dr has high sequence homology with MutY_Ec (Fig. 1). This is the first demonstration that an enzyme involved in repairing oxidized bases of D. radiodurans can complement E. coli mutants. We also demonstrated biochemically that MutY_Dr has MutY_Ec-like activities. Thus, the biological significance of MutY_Dr is similar to that of MutYEc in terms of removal of adenines misincorporated opposite GO lesions and reduction of C · GA · T transversions in the D. radiodurans R1 genome.

Biochemical activities of MutY_Dr resemble activities of MutY_Ec. Like the MutY_Ec protein, purified recombinant MutY_Dr expressed in E. coli exhibits adenine glycosylase activity with A/G, A/C, and A/GO mismatches and weak guanine glycosylase activity with G/GO mismatches. The substrate specificity and kinetic parameters of MutY_Dr are similar to those of MutY_Ec. An A/C mismatch substrate is a better substrate and a G/GO mismatch substrate is a worse substrate for MutY_Dr than for MutY_Ec. Although the K_m and V_max values for MutY_Dr with A/G mismatches are slightly higher than the corresponding MutY_Ec values, the specificity constants (K_cat/K_m) with the same mismatches are similar for the two enzymes. The binding affinities of MutY_Dr with all of the substrates tested are slightly lower than those of MutY_Ec. It is interesting that the affinities of MutY_Dr and MutY_Ec with the AP/G product were 5.5- and 2-fold fold higher, respectively, than those with the A/G substrate. The tighter binding to AP/G may recruit other enzymes to complete the repair process.

MutY_Dr has much less AP lyase activity than MutY_Ec. First, very limited DNA strand cleavage was observed if the product was not heated at 90°C. Piperidine treatment significantly increased strand cleavage by - and -elimination (Fig. 5). Second, when AP/GO-containing DNA was used as the substrate, as the concentration of MutY_Dr was increased, the amount of the -elimination product did not increase compared with the control (enzyme dilution only). Third, MutY_Dr could form only a weak covalent protein-DNA complex in the presence of sodium borohydride, while the catalytic activity of MutY_Dr was similar to that of MutY_Ec. This may have been due to an arginine residue that is present in MutY_Dr at the position corresponding to MutY_Ec Lys142, which forms the Schiff base with DNA. Thus, MutY_Dr most likely belongs to the group of monofunctional DNA glycosylases.

In E. coli, MutY, MutM, and MutT are involved in defending against the mutagenic effects of GO lesions (32, 45), and similar mechanisms to protect cells from the deleterious effects of GO are present in human cells. Homologs of putative MutY, MutM, and MutT proteins are present in D. radiodurans (27, 50). An activity similar to MutM_Ec activity has been detected in D. radiodurans R1 (4, 42). In contrast to MutM_Ec (8), MutM_Dr is able to excise GO from A/GO mismatches at a detectable rate (42), and the excision rates for 2,6-diamino-4-hydroxyl-5-formamidopyrimidine (FapyGua) and 4,6-diamino-5-formamidopyrimidine (FapyAde) are significantly greater than that for GO when these lesions are paired with C (42). Remarkably, there are 21 potential MutT homologs called Nudix hydrolases in the D. radiodurans R1 genome (27, 50). The D. radiodurans R1 Nudix hydrolases contain the conserved Nudix box (GX₅EX₇REUXEEXGU, where U is usually I, V, or L) and can hydrolyze nucleoside diphosphate derivatives (55). However, it is unlikely that many of these Nudix hydrolases are functional homologs of MutT_Ec because several of them cannot complement an E. coli mutT strain (55). Bauche and Laval (4) reported that MutY-like activity could not be detected in crude extracts of D. radiodurans R1. Our study showed that D. radiodurans R1 does have MutY_Dr activity that removes adenines from A/G- and A/GO-containing DNA and can complement an E. coli mutY mutation. Thus, MutY_Dr can protect D. radiodurans from GO mutational effects. However, no specific properties of MutY_Dr were found to be drastically different from MutY_Ec properties and to contribute to the high resistance to ionizing radiation and oxidative stress of the organism. On the other hand, MutY_Dr may have undiscovered activities that are different from MutY_Ec activities. So far, the reason(s) for the high tolerance to oxidative stress remains unknown. Comparison of repair and recombination enzymes of D. radiodurans and another highly radiation-resistant bacterium, Rubrobacter radiotolerans, may shed some slight on this.

In view of the fact that MutM_Dr can excise GO from A/GO mismatches and cleave at AP sites (42), it is important that MutY_Dr binds tightly to A/GO and AP/GO. MutY_Dr should be the preferred enzyme to act on A/GO mismatches in which adenines are misincorporated opposite template GO. After the removal of adenines from A/GO mismatches by MutY_Dr, the remaining AP/GO is a substrate for MutM_Dr (4). If MutY_Dr were to release this product before repair synthesis, MutM_Dr would incise both the AP site and the GO lesion, resulting in a double-stranded break. Strong binding to AP/GO by MutY_Dr minimizes this potential lethal action. The tight binding of MutY_Dr to the catalytically inactive substrate T/GO and weak substrate G/GO may also have biological significance. It has been shown that MutY_Ec can attenuate the MutM_ec glycosylase activity (20). Similar to the E. coli MutY-MutM interaction, the binding of MutY_Dr to T/GO and G/GO may inhibit MutM_Dr activity with these substrates. It remains to be determined whether MutM_Dr can act on T/GO and G/GO mismatches. The modulation of MutM activity is especially important if T/GO and G/GO mismatches arise from misincorporation of T and G opposite oxidized guanines on the template strand and if T/GO mismatches are derived from deamination of 5-methylcytosine opposite GO. The removal of GO from T/GO and G/GO mismatches, when GO is on the parental strand, can lead to G · CA · T transitions and G · CC · G transversions, respectively. Hence, it is reasonable that MutM_Dr activity with these unfavorable substrates is inhibited.