INTRODUCTION

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The proteins encoded by bacterial division and cell wall (dcw) gene clusters are essential for viability (16). Significant progress has been made in recent years in understanding the genes involved in cell wall biosynthesis and division (5, 6, 7, 11, 32, 62). Fifteen genes located at 2 min on the Escherichia coli chromosome have been identified and are called the dcw cluster (4, 64). The genes of this cluster are tightly packed, some overlapping, and are oriented in one direction (4, 64). Although the dcw cluster is highly conserved among evolutionarily diverse bacterial species (48), some variations in the clusters have been reported. These variations include the location of some dcw genes at separate chromosomal locations, such as ftsW, murF, and ddlA of Staphylococcus aureus (48), and the addition of species-specific genes within the dcw cluster, such as spoVD and spoVE of Bacillus subtilis (12, 31). In the case of these B. subtilis sporulation genes, spoVD (12) and spoVE (31) have homology to the dcw cluster genes pbpB and ftsW, respectively. Although not essential for vegetative growth, these genes are essential for sporulation (12, 31).

Since the products of the dcw genes encode proteins involved in peptidoglycan synthesis and cellular division, it is necessary to use conditionally lethal mutants to study these genes (5, 6, 11, 16, 27). Moreover, since they are typically expressed at low levels, it has been difficult to examine their regulation and to map their promoter elements (13). However, using promoter reporter constructs, a putative promoter (P_mra) upstream of the E. coli dcw cluster has been identified. This promoter is required for transcription of at least the first nine genes of the cluster (yabB to ftsW) and may provide the majority of the transcript for the downstream genes (30, 43).

Neisseria gonorrhoeae and N. meningitidis are important, human-specific pathogens and are causative agents of gonorrhea and of meningitis and septicemia, respectively. MtrR is a transcriptional regulator, in gonococci, of both the mtr operon and the far operon, which encode efflux pump proteins (28, 39). While investigating the regulation of the mtr efflux system by MtrR in N. gonorrhoeae, we performed a search of the gonococcal genome sequence database for the recognition site of MtrR (40) in an attempt to identify other genes that might be subject to its regulation. A potential MtrR-binding sequence was found upstream of an open reading frame (ORF) with homology to yabB of E. coli (4). A homologue of yabB is the first gene of the dcw cluster in all of the bacterial species studied to date (4, 13, 20, 38, 48, 64). Since the dcw cluster of the pathogenic Neisseria spp. had not been previously described at that time, we decided to investigate this gene cluster in the pathogenic Neisseria spp. A recent report (21) has described the gonococcal dcw cluster to some extent, although two ORFs (ftsL and the homologue of NMB0417) were not identified and a hypothetical gene was defined as the most 3' gene of the cluster, rather than the conserved ftsZ. We report that while the dcw clusters of gonococci and meningococci are broadly similar to those described in other species, they contain three additional ORFs internal to the dcw cluster that are not present in the dcw clusters of other the bacterial species studied to date. We demonstrate that these ORFs do not have features of recent horizontal acquisition and that they are all transcribed. One of these ORFs, dca, has features suggestive of phase variability associated with polymorphisms between strains and is associated with natural competence in the gonococcal strains, although not in the meningococcal strains tested.

	MATERIALS AND METHODS

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Bacterial strains and growth conditions. We used N. gonorrhoeae strains FA19 and FA1090 (kindly provided by P. F. Sparling and J. Cannon, respectively, of the University of North Carolina School of Medicine, Chapel Hill). N. meningitidis invasive disease isolate strains (NMB [capsular serogroup B], 0929 [serogroup Y], and 2633 [serogroup Y]) and six commensal Neisseria spp. (N. flavescens, N. lactamica, N. macacae, N. polysaccharea, N. sicca, and N. subflava) were kindly provided by D. Stephens (Emory University School of Medicine, Atlanta, Ga.). A transformant of strain FA19 containing an insertional inactivation and deletion in the recA gene was constructed by transformation using pC68a (kindly provided by C. Gibbs and T. Meyer), which contains recA::ermC. All strains were propagated on GCB agar or in GCB broth (Difco Laboratories, Detroit, Mich.) containing the Kellogg supplements and ferric nitrate (36) at 37°C under 3.8% (vol/vol) CO₂. Growth in liquid media employed nonpiliated, transparent colony variants with growth monitored by determining the optical density at 600 nm. Spontaneous streptomycin-resistant (Str^r) mutants of gonococci were selected by plating 10⁹ CFU of strain FA19 onto GCB agar plates containing 100 µg of streptomycin per ml. Str^r mutants of meningococci were made by transformation of strain NMB with Str^r gonococcal chromosomal DNA. Transformants were selected on GCB agar plates containing either 100 µg of streptomycin per ml or 1 µg of erythromycin per ml. All antimicrobial agents were obtained from Sigma Chemical Company (St. Louis, Mo.).

PCR amplification, plasmid preparation, and DNA sequencing. Chromosomal DNA extractions were performed using the method of McAllister and Stephens (41). PCR from chromosomal DNA was performed as described previously (29). Plasmids were purified using the QIAprep Spin Miniprep Kit according to the manufacturer's instructions (QIAGEN, Valencia, Calif.). DNA sequencing of PCR products and plasmids was performed using automated sequencing by the Emory DNA Sequencing Core Facility (Atlanta, Ga.) using a PE Applied Biosystems 377 automated DNA sequencers (Foster City, Calif.). Sequencing was carried out by the modified method of Sanger et al. (50) using BigDye reaction chemistry (ABI PRISM; PE Applied Biosystems, Norwalk, Conn.). PCR products for sequencing of dca were generated with combinations of oligonucleotide primers orfA#1, orfA#3, orfA#5, orfA#6, and murE#4 (Fig. 1 and Table 1). DNAStar was used for nucleotide and amino acid sequence analysis and alignments (8). Protein topology predictions were generated using the algorithm of von Heijne (60, 61), as implemented by TopPredII (10), and PSORT (45).

View larger version (36K): [in this window] [in a new window]   FIG. 1.   Comparison of the dcw clusters from N. gonorrhoeae, N. meningitidis strain Z2491 (46), N. meningitidis strain MC58 (59), E. coli (4, 64), H. influenzae (20), B. subtilis (13, 38), S. aureus, and E. faecalis (48). All genes are transcribed from left to right. Due to variations in nomenclature between the species, functionally conserved genes have different names; the penA, ftsI, and pbp genes are homologues, as are the yab and yll genes. The first two ORFs of the dcw clusters in this figure are named based on the consistent designations of these ORFs in GenBank. The spoVD and spoVE genes of B. subtilis are homologues of pbpB and ftsW, respectively, and the proteins they encode have a sporulation-specific function related to that of the PbpB and FtsW function in vegetative growth (12, 31). NMA and NMB annotation numbers are indicated below each ORF (46, 59). White boxes in N. gonorrhoeae and N. meningitidis represent ORFs not seen in other dcw clusters. The NMB0417 and NMB0419 homologues are of unknown function and are therefore not named in N. gonorrhoeae. The relative positioning of the frameshift in dca in N. meningitidis is indicated (fs). The relative positions of the oligonucleotides from Table 1 are shown (arrows). PE, yabBPE; E4, murE#4; A1, orfA#1; A3, orfA#3; A4, orfA#4; A5; orfA#5; A6, orfA#6; B3, dcaB#3; B4, dcaB#4; C1, dcaC#1; C2, dcaC#2. Apparent overlap is due to the limitations of the figure.

DNS. A DNA sequence can be considered to consist of a string of dinucleotides. Analysis of the proportions of dinucleotides in large sequences has revealed that species differences are consistently present in genome sequence compositions (34, 35). This approach has been used to identify regions of horizontally transferred DNA in N. meningitidis MC58 (59). We used a modification of this methodology that addresses single ORFs (44; J. Mirsky, N. J. Saunders, J. F. Peden, and S. Jarvis, unpublished data). We have used dinucleotide signature analysis (DNS) to evaluate the possibility that the ORFs within the dcw cluster were acquired by recent horizontal transfer of DNA.

Nucleotide sequence searches. The Basic Local Alignment Search Tool (BLAST) (1) was used to search publicly available microbial genome sequences and GenBank. The sequences of yabB, yabC, ftsL, penA, murE, dca, murF, the homologue of NMB0417, mraY, the homologue of NMB0419, murD, ftsW, murG, murC, ddlB, ftsQ, ftsA, and ftsZ for gonococci were obtained from the N. gonorrhoeae Genome Sequencing Project at the University of Oklahoma (http://www.genome.ou.edu/gono.html). The corresponding meningococcal sequences from serogroup A strain Z2491 were obtained from the Sanger Centre. This sequence data was produced by the N. meningitidis Sequencing Group at the Sanger Centre and can be obtained online (http://www.sanger.ac.uk/Projects/N_meningitidis/) (46). The meningococcal sequence from serogroup B strain MC58 was produced by The Institute for Genomic Research (http://www.tigr.org/tdb/CMR/gnm/htmls/SplashPage.html) (59). The sequence of dca from N. meningitidis serogroup C strain FAM18 was produced by the Sanger Centre (http://www.sanger.ac.uk /Projects/N_meningitidis/seroC.shtml). GenBank was accessed through the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/). The sequences of the Actinobacillus actinomycetemcomitans HK1651 dca homologues were obtained from the University of Oklahoma (http://www.genome.ou.edu/act.html). The sequence of the Salmonella enterica serovar Typhi strain CT18 dca homologue was obtained from the Sanger Centre (http://www.sanger.ac.uk/Projects/S_typhi/).

Primer extension analysis. Total RNA was prepared using the hot-phenol method (3). Primer extension products were generated from the yabB-specific oligonucleotide, yabBPE (Table 1), hybridized to 20 µg of total RNA. The DNA sequence was generated using the same oligonucleotide as a primer and therefore corresponds to that of the mRNA (28).

RT-PCR. RNA was prepared as for primer extension analysis. cDNA was generated from 1 µg of RNA reverse transcribed with SuperScript II RNase H reverse transcriptase (Gibco-BRL, Grand Island, N.Y.) and ORF-specific oligonucleotide primers orfA#4, dcaB#4, and dcaC#2 (Table 1). The cDNA was PCR amplified with oligonucleotide primer combinations of orfA#4 and orfA#1, dcaB#4, and dcaB#3 and dcaC#2 and dcaC#1 (Fig. 1 and Table 1). In order to detect contaminating DNA in the RNA preparation, control reverse transcription-PCR (RT-PCR) reactions were conducted in the absence of reverse transcriptase.

Southern blotting. A dca-specific probe of 1.6 kb was generated by PCR amplification of FA19 chromosomal DNA with orfA#1 and orfA#3 (Table 1) and labeled using the Boehringer-Mannheim Non-radioactive DNA Genius 2 Labeling Kit according to the manufacturer's instructions (Indianapolis, Ind.). Three micrograms of chromosomal DNA, determined using a Beckman EU65 spectrophotomer (Fullerton, Calif.) at A₂₆₀, was digested with ClaI, separated by 0.6% (wt/vol) agarose gel electrophoresis, and blotted to a 0.45-µM (pore-size) MagnaGraph nylon transfer membrane (Micron Separations, Inc., Westboro, Mass.). Hybridization, washes, and detection were performed as described in the Genius System User's Guide for Membrane Hybridization using anti-digoxigenin alkaline phosphatase conjugate and CSPD (Boehringer Mannheim).

dca-inactivation and transformation. dca was PCR-amplified from genomic DNA of strain FA19 using gene-specific primers (orfA#1 and orfA#3) and inserted into the pCR 2.1-TOPO vector using the TOPO TA Cloning Kit according to manufacturer instructions (Invitrogen, Carlsbad, Calif.). dca was excised with EcoRI and cloned into pUC19 (New England Biolabs, Beverly, Mass.). For construction of pLS1, the nonpolar aphA-3 cassette (42) was inserted into the NsiI site 1,319 bp into dca, disrupting the gene at the 3' end. To create pLS3, a PCR product of dca was created that would include 349 bp of DNA upstream of the BssHII site, 62 bp within dca using primers murE#4 and orfA#3. This product was then inserted into the pCR 2.1-TOPO vector, excised with EcoRI, and inserted into pUC19, as for pLS1. This construct was then cut with BssHII and NsiI, deleting 1,257 bp from dca, and the aphA-3 cassette was inserted. Restriction endonucleases were obtained from New England Biolabs (Beverly, Mass.). The pLS1 and pLS3 constructs were used as templates for PCR. Piliated gonococci (strain FA19) were transformed with PCR products as described by Gunn and Stein (26). Kanamycin-resistant (Km^r) transformants were selected using 50 µg of kanamycin (Sigma) per ml. Sequencing of the representative transformants confirmed the insertion of the aphA-3 cassette in strains LS1 and LS3 and the deletion of 1,257 bp from dca and that this transformation produced no changes in the upstream murE gene in strain LS3 (data not presented). RT-PCR studies showed no detectable polar effect of either the aphA-3 cassette or the deletion on the transcription of the downstream murF (data not presented). These transformants, along with strains FA19 and FA19 recA::ermC, were tested for natural competence by the method of Gunn and Stein (26) and by the agar overlay procedure of Sparling et al. (55) using chromosomal DNA containing the Str^r marker.

Accession numbers. The GenBank accession numbers for dca are as follows: N. gonorrhoeae strain FA19, AF195057; N. meningitidis strain NMB, AF195058; N. meningitidis strain 2633, AF195059; and N. meningitidis strain 0929, AF195060.

	RESULTS

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Identification and organization of the dcw gene cluster in gonococci and meningococci. The dcw gene cluster of N. gonorrhoeae was originally identified in a search of the FA1090 genome sequence database for sites with homology to the MtrR regulator consensus binding sequence (40). One putative MtrR-binding site (18 of 31 bases identical) is located 883 bp 5' of an ORF predicted to encode a protein with homology to the yabB-encoded protein of E. coli (32.9% amino acid similarity over the whole protein) (4). Subsequent experiments failed to demonstrate specific binding of MtrR to this site (data not presented), suggesting either that MtrR does not bind to this site or that additional cofactors or sequence determinants are required for its binding. However, the DNA sequence 3' of the putative yabB homologue includes an additional 17 tightly packed ORFs orientated in the same direction (Fig. 1), ending with ftsZ, usually ascribed as the most 3' gene of the dcw cluster (48). Of the 18 ORFs in this cluster, 15 encode putative proteins with homology to those encoded by dcw genes of other bacterial species (Fig. 1). These have between 28.4 and 55.3% amino acid similarity with the corresponding E. coli gene products, except for ftsL (17% amino acid similarity). This is consistent with ftsL frequently being the least-conserved dcw gene between different species. For example, although only 16% amino acid identity exists between the E. coli and B. subtilis genes, Daniel et al. (14) recently named the B. subtilis gene ftsL on the basis of its position in the dcw cluster, the ORF length, and the predicted protein features. Using this approach and the homologies to E. coli proteins, we were able to ascribe gene identifications to 15 of the 18 genes in the pathogenic Neisseria spp. Of the genes in this cluster, only penA, encoding penicillin-binding protein 2, and ftsZ, encoding cell division protein FtsZ, have been previously investigated in the Neisseria spp. (49, 56, 57). These ascribed identifications are consistent with the combined annotations of the recently published N. meningitidis genome sequences, including the annotation of NMB0412 as ftsL (46, 59). Recently, Francis et al. (21) published a description of the dcw cluster of N. gonorrhoeae, but our interpretation differs from theirs in several respects, including the definition of the 3' end of the cluster as the conserved ftsZ rather than an undefined ORF, the number of genes in the cluster as 18 rather than 17, the identification of ftsL, and the identification of an additional novel ORF within the cluster, the homologue of NMB0417. Additionally, the present work provides a comparative analysis of the dcw cluster from the three sequenced pathogenic Neisseria spp. strains, including N. meningitidis strains MC58 and Z2491 (Fig. 1).

Sequence composition of the additional neisserial ORFs within the dcw gene cluster. The pathogenic Neisseria spp. are naturally transformable, and this can lead to the acquisition of genes from both related and unrelated bacterial species (51). Given that the pathogenic Neisseria spp. examined contain ORFs within the dcw cluster that are not present in other bacterial species, we sought to investigate the evolutionary history of these genes by determining whether they have features of recent horizontal acquisition. The dcw cluster of N. meningitidis strain MC58 was analyzed using a modification of DNS that can assess single ORFs (44; Mirsky et al. unpublished) (Fig. 2). This analysis failed to detect evidence of recent interspecies horizontal acquisition of any of the genes in the dcw cluster, including the additional ORFs. The most atypical of the ORFs are murF and ftsA, but the degree of divergence is less than that normally associated with horizontally transferred genes in this genome, such as sodC (37) (Fig. 2). The short nature of ftsL (264 bp) and NMB0417 (177 bp) is sufficient to account for their elevated values in this analysis due to a sampling effect, and the moderate elevation of the NMB0419 homologue (381 bp) can be accounted for on a similar basis.

View larger version (28K): [in this window] [in a new window]   FIG. 2.   Dinucleotide signature of the genes within the dcw cluster. The figure shows the divergence of the dinucleotide signature for each gene within the dcw cluster in N. meningitidis strain MC58 from the mean value determined for the complete genome sequence (59) using the method of Mirsky (44). Genes which have been recently horizontally transferred tend to have a divergence of greater than 16% using this type of analysis in this genome (N. J. Saunders, unpublished data), once elevations due to sampling in short ORFs has been accounted for. For example, the sodC gene, which is believed to have been horizontally acquired from or via H. influenzae (37), has a dinucleotide signature divergence from the mean of 25% (black bar). Striped bars indicate the three additional dcw genes: dca, NMB0417, and NMB0419.

dcw cluster-associated ORF dca. We selected dca, which is located between murE and murF in both gonococci and meningococci, for further investigation on the basis of its homology to hypothetical proteins present in other gram-negative species and the presence of a homopolymeric tract suggestive of phase variation (see below). The sequence of dca (1,647 bp) from gonococcal strain FA19 was determined. dca encodes a predicted protein of 548 amino acids with five transmembrane domains located within the N-terminal region between residues 16 and 174 and an N-terminal signal sequence of 35 amino acids. Similar hypothetical proteins are present in other microbial genome sequences, but they appear to be restricted to gram-negative bacteria. Alignment of the gonococcal sequence with related proteins of A. actinomycetemcomitans, E. coli (ybiP), H. influenzae (HI1005), and serovar Typhi are shown in Fig. 3. Protein structural predictions, obtained by using TopPredII, suggest that these homologues have a structure and conformation similar to that of the predicted dca product.

View larger version (108K): [in this window] [in a new window]   FIG. 3.   dca homologues. Alignment of the predicted amino acid sequence of dca from N. gonorrhoeae FA19 (N.g.) with hypothetical proteins from A. actinomycetemcometans (A.a. 1 and A.a. 2), H. influenzae HI1005 (H.i.) (20), E. coli ybiP (E.c.) (4), and serovar Typhi (S.t.). Identical residues are indicated by black boxes.

View larger version (19K): [in this window] [in a new window]   FIG. 4.   Alignment of the repeat region predicted to mediate phase variation within dca from the gonococcal strains FA1090 and FA19 and the meningococcal strains Z2491, MC58, NMB, 2633, and 0929. The frameshift caused by the presence of nine guanines, as in Z2491, MC58, and NMB, brings the underlined termination codon in frame, resulting in a premature termination of the ORF. aa, amino acids.

Insertional-inactivation of dca. Based on its location within the dcw gene cluster, we hypothesized that the dca-encoded protein might participate in the processes of cell division and/or cell wall biosynthesis. In order to investigate this possibility, we constructed two separate nonpolar mutations within dca using the Km^r-containing aphA-3 cassette and then transformed them into their original chromosomal locations in strain FA19. Transformants of strain FA19 were readily obtained with the DNA sequence generated from pLS1 and pLS3, indicating that dca is not an essential gene. The transformant generated with the pLS1 sequence contains the aphA-3 cassette inserted into the NsiI site 1,319 bp into dca, disrupting the gene at the 3' end. The transformant generated with the sequence from pLS3 has 1,257 bp deleted from dca (between the BssHII and NsiI sites), leaving only 62 bp of dca before the insertion of the aphA-3 cassette. PCR amplification and sequencing (data not presented) was used to confirm that the aphA-3 cassette had been inserted into dca at the predicted sites within the transformants, which were termed LS1 and LS3, respectively. In transformant LS3, 1,257 bp were deleted from dca. This deletion and replacement with the nonpolar aphA-3 cassette had no detectable effect on transcription of the downstream murF gene as determined by RT-PCR (data not presented).

Natural competence for transformation of dca mutants. In the course of investigating the phenotypes of the mutants, an apparent deficiency in transformation was noted in strain LS3. To determine whether strains LS1 and LS3 were deficient in transformation, we selected piliated variants (on the basis of colony morphology with the presence of pili confirmed by electron microscopy) and examined their capacity to be transformed with chromosomal DNA, using piliated variants of parental strain FA19 and FA19 recA::ermC as controls. As shown in Table 2, we found that strains FA19 and LS1 were readily transformable (frequencies of 10³) using a chromosomal DNA marker (streptomycin resistance), while LS3 was reproducibly nontransformable (frequency of <10⁸). In parallel experiments, a recA::ermC insertional mutant of strain FA19 was also nontransformable (frequency of <10⁸) (Table 2). The association between the transformation defect in strain LS3 and the dca::aphA-3 mutation was confirmed by analyzing three independent dca::aphA-3 transformants of strain FA19 obtained during the original construction of LS3. All three transformants displayed the same transformation defect exhibited by the first LS3 strain (frequency of <10⁸). We also examined the capacity of these piliated strains to be transformed with donor DNA using the agar overlay transformation procedure of Sparling et al. (55). However, regardless of the liquid or plate transformation conditions, transformants of strains LS3 or FA19 recA::ermC could not be recovered (frequencies of <10⁸).

3

	DISCUSSION

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Horizontal gene transfer is thought to be an important component of bacterial adaptation and evolution. The Neisseria spp. are naturally competent for transformation (9, 54), meaning that they are capable of taking up external DNA and incorporating it into their chromosome by homologous recombination. Natural transformation predominantly occurs within populations of cells that are of the same or closely related species, but it is probably also involved in the acquisition of new genes from unrelated species. In Neisseria spp., species-specific targeting is largely mediated by the presence of a 10-bp uptake signal sequence (GCCGTCTGAA) which is frequently located 3' of ORFs, (18, 25). Transformation facilitates the generation of mosaic genes through recombination between existing alleles, new gene acquisition, and possibly repair of genes whose function has been lost by mutation. Recognized examples of this process in Neisseria spp. include (i) the argF gene of N. meningitidis, which includes sections derived from a species similar to N. cinerea and from an additional unidentified source (65); (ii) the generation of Opa variants within a population (2); (iii) the acquisition of penicillin resistance through the incorporation of sequences from N. flavescens and N. cinerea (56, 57, 58); and (iv) the acquisition of foreign genes from other species (37). This is also likely to be the primary route by which silent pilus cassettes are provided for antigenic variation by recombination with the expressed pilE gene (53).

Natural transformation is an integral part of neisserial biology and involves several genes. In addition to the requirement for the expressions of pili (22, 54), other proteins have been shown to have a role in natural transformation in the Neisseria spp. These include Tpc, mutants of which form tetracocci deficient in epithelial cell invasion and competence (23); ComA, an inner membrane protein which may be involved in translocation of DNA into the cytoplasm (19); ComL, a peptidoglycan-bound lipoprotein, mutants of which are significantly decreased in transformation (24); and PilT, which is involved in twitching motility and DNA uptake (63). Our results indicate that dca is also essential for transformation in gonococci. The fact that LS1 retained its competence indicates that the 3' end of dca is not involved in this function. In separate experiments we also evaluated the capacity of strain FA1090 and FA1090 dca::aphA-3 (obtained using donor DNA from LS3) to be transformed with gonococcal DNA. Using an erythromycin resistance marker from strain KH15 (as FA19 but mtrR-171 [28]), we found that dca was also required for transformation in strain FA1090 (frequency of <10⁸).

The ability of the meningococcal strain NMB to retain its competence suggests that N. meningitidis transformation occurs through an alternate and/or additional pathway. Alternatively, there may be functional redundancy with the dca-encoded product or the dca-associated system. Although no homologues to dca were found in either the N. meningitidis strain Z2491 or MC58 genomes in addition to dca itself and only one band appears on Southern blots probed for dca, this does not rule out the possibility of more diverse proteins with similar functions. Similar inactivation of dca in serogroup Y meningococcal strain 0929, which has a (G)8 HPT (Fig. 4), and thus a full-length dca ORF, also did not affect competence (frequency of 10³). The fact that this ORF is retained in the chromosome and is putatively phase variable suggests that this protein has an additional function. Due to the proximity of the termination codon to the HPT (Fig. 4) and the presence of strains with both (G)8 and (G)9 sequences, this gene is a strong candidate for phase variation (52). The phase variation of this gene is currently being further investigated.

The insertion-deletion mutation in the dca mutants created for the competence experiments was generated using an aphA-3 cassette designed by Ménard et al. (42) to be nonpolar. It is demonstrated here that the mutation in dca did not affect transcription of the 3' gene, murF, and, given that the conserved dcw genes are essential, the effects on their transcription and translation should negatively impact the organism. Due to the fact that the functions of the homologues of NMB0417 and NMB0419 are not known, the possibility still exists that this mutation is having an effect on the expression or function of those gene products, effecting the competence phenotype. Classical complementation studies, which could rule out this possibility, cannot be done due to the lack of genetic tools for complementation in the pathogenic Neisseria spp.

In summary, the dcw cluster of the pathogenic Neisseria spp. is highly homologous to those seen in other diverse organisms (Fig. 1). Similar to E. coli, there is a ⁷⁰ promoter located 5' of yabB. It is not known if this one promoter drives transcription for the entire cluster, regardless of additional transcription from internal promoters, as P_mra is thought to do in E. coli. The three unique ORFs found within the highly conserved dcw cluster do not have features of recent horizontal acquisition (Fig. 2) but rather are integral parts of the genome. Unlike B. subtilis, which also has additional genes in its dcw cluster (12, 31), these are not homologues of other dcw genes. dca, one of these additional genes, encodes a putative inner membrane protein that has similarity to hypothetical inner membrane proteins present in several gram-negative bacteria that are located outside of their dcw clusters (Fig. 3). DNA sequencing of this ORF from several gonococcal and meningococcal strains revealed the presence of a homopolymeric tract of G's of variable length in this ORF, which suggests that this gene is phase variable (Fig. 4). We observed that while loss of the dcw cluster-associated ORF dca had no significant effect on peptidoglycan synthesis and growth rate, it had a profound effect on transformation, which could be localized to the 5' region of the reading frame (Table 2). Thus, its loss rendered gonococci incapable of being transformed by chromosomal DNA, although meningococcal transformation was unchanged by similar mutagenesis of dca. We propose that Dca is a necessary component of the transformation machinery of gonococci, perhaps functioning to facilitate movement of DNA across the cytoplasmic membrane. The lack of necessity of Dca in the meningococci may suggest the presence of an alternate pathway in this species and that Dca may have additional, as yet undefined, functions.