INTRODUCTION

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The ribosomal proteins of Escherichia coli were systematically characterized in the beginning of the 1970s, and a unified nomenclature, S1 through S21 for the 30S subunit and L1 through L34 for the 50S subunit, was proposed on the basis of their behavior in two-dimensional gel electrophoresis (17, 44, 45, 46). Using a modified and improved method, termed radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis (RFHR 2-D PAGE), we discovered four additional proteins, proteins A, B, C, and D, that were associated with E. coli ribosomes (38, 39, 40).

The gene for protein A (40) was located between infC (initiation factor 3) and rplT (ribosomal protein L20) (26, 27, 31) at 38 min, suggesting that the three constitute a new ribosomal protein operon in E. coli. The gene for protein B (40) was found to be identical to the protein X gene of the spc operon (8). Proteins A and B were consequently named L35 and L36 and their genes were named rpmI and rpmJ, respectively, in accordance with the standard nomenclature for E. coli ribosomal proteins and their genes. Protein C (41) is likely to be the full-length product of the rpmE gene, coding for L31, because the N-terminal amino acid sequence completely matches that of L31 and the molecular mass of protein C is larger than that of L31 by about 1,000 Da. More recently, L31 was proved to arise through cleavage of protein C by protease VII, an outer membrane enzyme (A. Wada, unpublished data).

Protein D is a small basic protein that can be isolated from the 30S subunit after dissociation of the 70S ribosome. It migrates much faster than S21 and to a point below the L32 spot upon RFHR 2-D PAGE of purified 70S ribosomal proteins. Thus, it is the smallest protein component of the 30S subunit of E. coli. When the 70S ribosome was dissociated into the core particle and split proteins by CsCl density gradient centrifugation (34), protein D was found in the soluble split-protein fraction (39). Furthermore, in simultaneous reconstitution experiments with 50S and 30S subunits, protein D was exclusively associated with the 30S subunit (39).

In this study, we have identified the gene encoding protein D by determining its N-terminal amino acid sequence and have analyzed the mechanism of control of its expression in terms of growth phase and the transcriptional factors involved. Protein D was named SRA (stationary-phase-induced ribosome-associated) protein.

	MATERIALS AND METHODS

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Bacterial strains, plasmids, phage, and culture conditions. E. coli K-12 strains, plasmids, and phage (Table 1) were grown in medium E (37) containing 2% Polypeptone and supplemented with 0.5% glucose (EP medium) at 37°C with shaking at 100 cycles/min. Cells were harvested in the middle exponential and/or stationary phase of cell growth and stored at 80°C until use.

Preparation of ribosomes, ribosomal subunits, and ribosomal proteins. Crude and high-salt-washed ribosomes were prepared according to the methods of Noll et al. (29) and Horie et al. (16). Subunits were isolated from the high-salt-washed ribosome preparation by sucrose density gradient centrifugation as described previously (38). Proteins were prepared by the acetic acid method of Hardy et al. (13), dialyzed against 2% acetic acid, lyophilized, and stored at 80°C until use.

RFHR 2-D PAGE and determination of copy number of SRA (protein D). Lyophilized ribosomal proteins (approximately 0.3 mg/gel) were analyzed by RFHR 2-D PAGE as described previously (38) with the following modifications: the volume of glacial acetic acid used in the sample charging buffer (50×) was 7.4 ml, and the gel thickness used was 2 mm. After the gels were stained with amido black 10B in 1% acetic acid and destained with 1% acetic acid, the protein spots on the gels were scanned with a PD 110 personal densitometer (Molecular Dynamics Co.). The copy number of SRA (protein D) was determined by calculating the optical density values obtained per unit of molecular weight and normalizing them with the average values for S18, S19, L32, and L33, each of which has one copy per ribosome (38). The standard deviations for these calculations were approximately 25%.

Amino acid sequence analysis of SRA. After electrophoresis, the SRA protein was blotted from the gel to a polyvinylidene difluoride membrane. Sequence analysis was performed using a model 477A protein sequencer (Perkin-Elmer Applied Biosystems) as described previously (42).

Gene locus and nucleotide sequencing. The amino acid sequence data obtained were used to design a degenerate probe: AAT/CCGT/CCAA/GGCT/C/ G/ACGT/CCAT/CATT/CCTGGG. The probe was used to screen Kohara  clone library clones (19). Positive clones obtained were 1G9 (277), 9B8 (278), and 10C7 (279), which are located at 33 min. A chromosomal insert in clone 277 was subcloned into either pUC118 or pUC119, and the fragment containing the sra gene was sequenced by the dideoxy method (24).

Construction of pF13(Psra-lacZ). Five regions upstream of the ATG initiation codon of the sra gene as well as 39 bp downstream of it were amplified by PCR. The primers used contained a KpnI site to facilitate cloning into the pMS4342 plasmid vector for operon fusion. The inserts thus cloned were recombined in vivo into phage vector pF13 (15, 50), and the resulting phage, pF13(Psra-lacZ), was lysogenized into strain KY1461. The forward primers used and the distances (base pairs) from the ATG initiation codon (in parentheses) are as follows: 5'-CGGGTACCGGATATTCCGAATAAAATCCGG-3' (595), 5'-GCGGTACCTCATGGTGTTAAAATATAAA-3' (455), 5'-GCGGTACCGAATCACTAGTTTACTTAT-3' (325), 5'-GCGGTACCCTGACCAAATGGGTTGAAGC-3' (200), and 5'-GCGGTACCCACGTGTTTCTTCGCTACGG-3' (62). The reverse primer, 5'-CGGTCGACCAGTCCAAGAATATGACGTGCC-3', contained a SalI site. The DNA of plasmid pKV7350 was used as a template in all PCRs. The correctness of the sequences of the PCR products was confirmed by nucleotide sequencing. Unless otherwise stated, pF13(Psra-lacZ) with the region 595 bp upstream of the ATG of sra was used throughout this work.

Measurement of the level of transcription of the sra gene. The level of transcription was estimated by measuring the -galactosidase activity of the lacZ reporter in each lysogen constructed as described above. For the integration host factor (IHF)- or cyclic AMP (cAMP)-defective mutant into which pF13(Psra-lacZ) could not be lysogenized, plasmid pFF6(Psra-lacZ) (18), a mini-F plasmid derivative carrying an ara-trp-lac fusion identical to that carried by pF13, was used instead.

Determination of -galactosidase activity. Aliquots (0.1, 0.2, or 0.5 ml) of a culture were mixed with Z buffer (0.9, 0.8, or 0.5 ml, respectively) in an ice bath. Cells were disrupted by the addition of chroroform and sodium dodecyl sulfate and were assayed for -galactosidase activity essentially as described by Miller (28). The average of four to eight assays is reported.

Construction of deletions of the sra gene. The regions flanking the sra gene were amplified by PCR using pKV7350 (pUC119-277L) as the template and 5'-TTTAACTCCTCAATCCTGTAGCTAG-3' and 5'-TTCATAACCATCAGTCCTCAATGAC-3' as the primers. The resultant PCR product was restricted with HincII and ligated into a kanamycin-resistant cassette with HincII sites at both ends (derived from plasmid pUC-4K). Whether plasmid pKV7351 (pUC119-277L-sra::kan) was correctly constructed or not was examined by nucleotide sequencing. The plasmid was then linearized with SphI and EcoRI and transformed into MG1655 cells carrying plasmid pBAD-, which increases recombination frequency (51). Kanamycin-resistant transformants were selected, and deletion of the sra gene in them was confirmed by PCR. Subsequently, P1 phage prepared on one of the transformants was used to transduce MG1655 cells, and kanamycin-resistant transductants were selected. The sra gene deletion was similarly confirmed by PCR, and the strain was designated MG1655sra::kan.

Primer extension. MG1655, MG1655sra::kan, and MG1655 carrying pKY7350 (pUC119-277L) were grown at 37°C in 10 ml of Luria-Bertani medium to the stationary phase of growth (2 h after cessation of the turbidity increase). Cells in 1 ml of culture were harvested by centrifugation at 5,000 × g for 15 min, and RNA was extracted using an RNA-DNA extraction kit (Qiagen Inc.). All solutions were treated with diethylpyrocarbonate (Sigma) prior to use. The concentration of RNA was estimated from the absorbance at 260 nm, and the quality and degree of degradation of RNA were assessed by electrophoresis on a 1.5% agarose gel containing 1.8% formaldehyde. The primer used for extension was complementary to the coding region of sra and was 5'-TTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCG-3'. For the primer extension assays, 10 pmol of a ³²P-end-labeled oligonucleotide primer and 40 µg of RNA template were mixed, heated at 65°C for 90 min, and annealed by allowing the mixture to cool slowly to room temperature. The extension reaction was performed according to the method described by Triezenberg (35). The reaction mixtures were boiled for 2 min and loaded onto a 5% denaturing polyacrylamide sequencing gel. The sequencing reaction was performed using a BcaBEST dideoxy sequencing kit (Takara) and 3.2 pmol of the same primer as that used for the primer extension reaction.

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

SRA is present exclusively in ribosomal particles. Strain W3110 was cultured and harvested at the stationary phase (after 24 h of growth), and cell extracts, crude ribosomes, high-salt-washed ribosomes, and the dissociated 30S subunit were prepared and analyzed by RFHR 2-D PAGE (Fig. 1). Subsequently, the quantity of SRA protein on each electropherogram was densitometrically determined as described in Materials and Methods. The copy number of the SRA protein was about 0.4 per ribosome in the cell extracts, and the level was maintained in the subsequent processes of ribosome preparation, including dissociation into subunits. These results indicate that SRA is strongly associated with 30S subunit particles not only in the exponential phase, as described previously (38), but also in the stationary phase. Free SRA protein was not detected in the soluble fraction after sedimentation of crude ribosomes by centrifugation at 165,000 × g for 90 min (data not shown). Therefore, we believe that SRA is an integral part of the ribosome, especially of the 30S subunit.

View larger version (112K): [in this window] [in a new window]   FIG. 1.   Association of SRA (protein D) with the 30S ribosomal subunit. Electropherograms from RFHR 2-D PAGE analysis of the ribosomal proteins in total cell extracts (A), crude ribosomes (B), high-salt-washed ribosomes (C), and 30S subunits (D) prepared from W3110 cells grown to the stationary phase. Protein D (SRA), S21, L32, and L33 spots are indicated.

The copy number of SRA is dependent upon the cellular growth phase. Cells of strain W3110 were harvested in the middle exponential to late stationary phase of growth, and the proteins prepared from crude ribosomes were analyzed by RFHR 2-D PAGE. The results shown in Fig. 2 demonstrate that the copy number of SRA increased from 0.1 in the middle exponential phase to 0.4 in the stationary phase. Cell viability remained constant at 70% or more during the 4 days of culturing (Fig. 2).

View larger version (19K): [in this window] [in a new window]   FIG. 2.   Cellular viability and copy number of SRA in the stationary phase. Cultures of W3110 cells were sampled at different times from the exponential phase through the stationary phase of growth. Optical density at 600 nm and colony-forming ability were determined, and the relative viability values obtained were normalized to that in the late exponential phase. Proteins extracted from harvested cells were analyzed by RFHR 2-D PAGE. The copy number of SRA was determined as described in Materials and Methods.

Amino acid sequence analysis of SRA. An RFHR 2-D PAGE gel of 70S ribosomal proteins was blotted onto a polyvinylidene difluoride membrane, and the SRA spot was subjected to protein sequence analysis. The sequence of the first 37 amino acid residues from the N terminus was MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPT. A protein with this sequence has not yet been identified among the ribosomal proteins or ribosome-associated proteins from E. coli or any other organism. We identified this protein in 1992 (unpublished data) when E. coli genome sequencing had not yet been completed.

Gene locus and DNA sequence of the sra gene. A mixed DNA probe (26-mer) (described in Materials and Methods) was synthesized based on the amino acid sequence of the SRA protein from the fourth asparagine so as to perform screening of Kohara clones (19). Clones 1G9 (#277), 9B8 (#278), and 10C7 (#279) were found to hybridize with the probe. They are all located at 33.6 min on the E. coli genetic map. A total of 2,257 bp containing the gene (138 bp) for SRA (i.e., sra) was sequenced using the dideoxy method (reference 24 and data not shown).

Construction and characterization of an sra deletion mutant. An sra disruptant (MG1655sra::kan) was constructed as described in Materials and Methods, and its disruption was confirmed by PCR and Northern and Western blot analyses (data not shown) (see below for Northern analysis). To analyze the phenotype of the mutant, it was grown at 37°C for 6 to 7 days in either Luria broth or EP medium along with the parental wild-type strain (MG1655). Samples of each culture were withdrawn at various times from the exponential phase through the stationary phase of growth, and colony-forming ability was tested. Very few differences were observed between the strains (data not shown).

The level of transcription of the sra gene is increased in the stationary growth phase. As described above, the copy number of the SRA protein per ribosome increased during the stationary phase. To elucidate the control mechanism for sra gene expression, we analyzed the level of expression of sra during the transition from the exponential phase to the stationary phase of growth by measuring -galactosidase activity with lacZ reporters.

View larger version (14K): [in this window] [in a new window]   FIG. 3.   Construction of phage pF13(Psra-lacZ) and growth-phase-dependent induction from the sra promoter. (A) Construction of phage pF13(Psra-lacZ). The sra promoter region was PCR amplified, digested, and cloned into plasmid pMS4342 digested with KpnI and SalI. The resultant sra-lacZ fusion was transferred to phage vector pF13 as described in Materials and Methods (see the text for details). (B) Growth-phase-dependent induction from the sra promoter. MG1655 cells lysogenic for pF13(Psra-lacZ) were grown at 37°C in EP medium for 6 days. Samples from cultures prepared in separate tubes were withdrawn at various times, and the optical density at 600 nm (OD600) and -galactosidase activity in Miller units were determined.

Effects of sigma S and global regulators on the transcription of sra. It is known that RNA polymerase with sigma S transcribes a large number of genes in the stationary phase. We examined whether the expression of the sra gene is dependent on sigma S. An rpoS mutant strain, KY1461 katF::tet (KY1471), as well as parental wild-type strain KY1461, carrying one copy of pF13(Psra-lacZ), was grown at 37°C in EP medium. The level of expression of sra in both exponential- and stationary-phase cultures was estimated by measuring -galactosidase activity. The level of expression of sra in the exponential phase was low in both the rpoS mutant strain and the wild-type strain. In contrast, when cultures entered the stationary phase, sra expression increased in the wild-type strain but not in the rpoS mutant strain, which showed only about 30% the sra expression of the wild-type strain (Fig. 4).

View larger version (31K): [in this window] [in a new window]   FIG. 4.   Effects of global regulators on the transcription of sra. Cells of KY1461 lysogenic for pF13(Psra-lacZ) and containing either rpoS, fis, hns, ompR, or relA and spoT mutations and cells of KY1461 wild type or containing cya or hip and him mutations and carrying pFF6(Psra-lacZ) were grown at 37°C in EP medium. Cultures in either the exponential phase (optical density at 600 nm, 0.4) or the stationary phase (after 24 h) of growth were assayed for -galactosidase activity, expressed relative to that of wild-type cells in the stationary phase.

Determination of the transcriptional start site of sra by primer extension. Based on the DNA sequence data alone, it was difficult to predict the promoter sequence of the sra gene. Therefore, its transcriptional start site was experimentally determined by primer extension using mRNA prepared from stationary-phase cells. The start site of the major transcript of sra was determined to be located at the 83rd C counted from the translational initiation codon (ATG) of sra (Fig. 5). The sra transcript was not observed in the stationary-phase culture of the sra deletion mutant strain. The 10 region for the sra promoter is likely to be 5'-TATGCT-3', and the 35 region is likely to be 5'-AGCACC-3'. Since no other transcripts were observed for the region about 600 bp upstream of the translational initiation codon, the promoter identified here is the major one for the sra gene. By Northern analysis, only one sra transcript was detected in the stationary-phase culture, but no transcript was observed in the exponential-phase culture. These results are consistent with the -galactosidase reporter assay results described above.

View larger version (53K): [in this window] [in a new window]   FIG. 5.   Identification of the transcriptional start site of sra. The transcriptional start site was determined by primer extension using the oligonucleotide primer described in Materials and Methods. Sequence ladders were generated by the dideoxy method with the same primer and pKV7350 as the template. RNA was extracted from cultures of MG1655 (lane 1), MG1655sra::kan (lane 2), and MG1655(pKV7350) (lane 3) grown at 37°C to the stationary phase. The nucleotide sequence of the sra promoter region is indicated to the right, and an arrow indicates the transcriptional start site of the major transcript.

Effects of upstream cis elements on sra transcription. Experimental determination of the transcriptional start site of the sra gene led to the prediction of the 10 and 35 regions of the sra promoter. Based on these data, we searched in the region upstream of the sra promoter elements for likely cis elements that might be recognized by global regulators of transcription. sra-lacZ fusion constructs containing regions 595, 455, 325, 200, or 62 bp upstream of the initiation (ATG) codon of sra (see Materials and Methods) were used for this purpose, and the -galactosidase activity of cell samples taken at various times was assayed (Fig. 6). The sra 62 construct showed very low activity, most likely because it lacked some of the promoter elements. The sra 200 and sra 325 constructs, which contained the sra promoter, were both active, but the sra 455 construct showed about 75% higher -galactosidase activity. These results suggested that the region from 455 to 325 bp might contain a cis element(s) that can be recognized by one or more of the global regulators that were shown to positively affect sra expression in the stationary phase (Fig. 4).

View larger version (12K): [in this window] [in a new window]   FIG. 6.   Search for cis elements in the region upstream of the sra promoter. KY1461 cells lysogenized with various pF13(Psra-lacZ) constructs were grown at 37°C in EP medium. Cultures in either the exponential phase (optical density at 600 nm [OD600], 0.4) or the stationary phase (OD600, 1.5) were subjected to a -galactosidase assay. The negative numbers indicate the construct endpoints measured from the A of the translation initiation codon ATG. The start sites and directions of transcription of sra and osmC are indicated by arrows.

Homologous genes in other bacteria. To determine to what extent the sra gene is phylogenetically conserved in other organisms, nucleotide and protein databases were searched by BLAST. Homologous genes were identified in Salmonella enterica serovars Typhimurium, Typhi, and Paratyphi and Klebsiella pneumoniae (Fig. 7A). The genes in these bacteria code for proteins of 47, 47, 41, and 46 amino acid residues, respectively. The N-terminal domain of the proteins predicted from these genes was particularly well conserved in all five bacteria. Moreover, by aligning the nucleotide sequences of the upstream region of the gene, the Shine-Dalgarno sequence, mRNA start site, and 10 and 35 regions identified in E. coli were found to be perfectly conserved in all the bacteria except for K. pneumoniae, in which the degree of conservation was lower (Fig. 7B). These data suggest not only that each of the four bacteria harbors a gene identical to E. coli sra but that it is transcribed in a fashion identical to that found in E. coli.

View larger version (79K): [in this window] [in a new window]   FIG. 7.   Comparison of SRA and its homologs. (A) Alignment of amino acid sequences of SRA proteins from E. coli (ECO), Salmonella serovar Typhimurium (STM), Salmonella serovar Typhi (STY), Salmonella serovar Paratyphi (SPA), and K. pneumoniae (KPN). Dark boxes indicate complete sequence identity in all, and light boxes indicate identity in three or four organisms. (B) Alignment of the nucleotide sequence of the region upstream of the sra gene. Shaded boxes indicate identity in at least four organisms. The asterisk, 10 and 35, and SD show the transcriptional start site, RNA polymerase consensus sequences, and Shine-Dalgarno sequence of the E. coli sra gene, respectively.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

In this paper, we have presented evidence indicating that the ribosome of E. coli contains a protein of unknown function, termed SRA, whose copy number increases in the stationary phase of growth. The relative copy number of the SRA protein remained constant throughout the ribosome purification steps, including washing with high concentrations of salts (Fig. 1). In addition, the protein bound exclusively to the 30S subunit in an in vitro simultaneous reconstitution experiment with both ribosomal subunits (39). Also, the protein was not detected in a free form in the supernatant after sedimentation of ribosomes by centrifugation. These data suggest that SRA is exclusively associated with ribosomes; hence, ribosomes must be the site of its function. Among the ribosomal proteins identified and characterized so far in various organisms, there are quite a few, including the S1 protein of E. coli, whose copy numbers are rather low. However, the copy number of SRA is even lower than that of S1, especially in the exponential phase of growth although, interestingly, it increases about fourfold during the transition to the stationary phase (Fig. 2). Taking these features into account, we adopted the name SRA.

The increase in the amount of the SRA protein is likely to reflect its unknown physiological role in the stationary phase. However, we were unable to discern any anomalies associated with the sra deletion mutation, not only during exponential growth but also upon prolonged culturing of the mutant. Thus, the precise function of the protein in relation to ribosomes remains to be investigated further.

RMF is highly expressed in the stationary phase and binds to the 50S subunits of 70S ribosomes. RMF plays a role in the dimerization of 70S ribosomes into translationally inactive 100S particles. It is believed that the loss of aminoacyl-tRNA binding is responsible for the reduced translational activity (43). The formation of 100S ribosomes and the levels of expression of RMF and SRA appear to be in parallel with each other over the entire growth cycle of E. coli. Nonetheless, an sra deletion mutant and its parental wild-type strain were indistinguishable from each other in terms of the expression of RMF and the formation of 100S ribosomes.

From the expression profile of sra, its transcription appears to be dependent on factors that are involved in the regulation of genes active in the stationary phase. Indeed, the -galactosidase assay of the sra-lacZ operon fusion indicated that the level of transcription of sra in the rpoS mutant was only about 30% the wild-type level in the stationary phase (Fig. 4). In accordance with these results, we observed by Northern analysis that transcription from the sra promoter was lower in the rpoS mutant (data not shown). Therefore, the promoter of sra is partly dependent on sigma S. Furthermore, global transcriptional regulators, such as FIS, IHF, cAMP, and ppGpp, were found to positively regulate directly or indirectly the transcription of sra during the stationary phase, and the region between 455 and 325 bp is likely to be responsible for the action of the regulators (Fig. 6). The low level of transcription of sra observed in the exponential phase with the wild-type strain was also the case with mutants defective in the global regulators mentioned above (Fig. 4). Therefore, these global regulators do not interfere with sra expression in the exponential phase.

Various global regulators have been demonstrated to have both upregulatory and down-regulatory effects on genes in E. coli. For instance, the cAMP-cAMP receptor protein (CRP) complex has been implicated as an activator of carbon starvation-responsive genes (32). Various genes, including rpoS and several sigma S-dependent genes, such as bolA, are negatively controlled by cAMP-CRP (20, 21). Other global regulators, such as IHF, H-NS, and FIS, are histone-like DNA-binding proteins with a variety of functions. IHF stimulates the expression of dps in the stationary phase in a sigma S-dependent fashion (2), and the level of IHF increases approximately 10-fold during the transition to the stationary phase. Mutants of hns generally have increased levels of sigma S-controlled genes during exponential growth, and the expression of sigma S is stimulated by a posttranscriptional mechanism in hns mutants (3). Thus, it is known that H-NS is a pleiotropic exponential-phase inhibitor of the expression of many stationary-phase-responsive genes. It is an abundant protein in growing cells, and its concentration further increases upon entry into the stationary phase (9, 36). FIS is a relatively abundant DNA-binding protein found in exponentially growing cells. FIS binds sites upstream of promoters for rRNA and tRNA genes and strongly stimulates their promoter activity (30). The level of ppGpp in cells increases under stringent growth conditions. ppGpp has pleiotropic effects on various genes. In a relA spoT double mutant, which is essentially devoid of ppGpp, the cellular sigma S content is greatly decreased because ppGpp has a positive regulatory action on sigma S (10). Thus, many global regulators, including cAMP, H-NS, IHF, and ppGpp, influence the expression of sigma S, which in turn may regulate the transcription of sra in the stationary phase. However, details of the mechanisms of fine control of sra expression in the exponential phase as well as in the stationary phase by sigma S, OmpR, FIS, IHF, cAMP, and/or ppGpp remain to be investigated further.

A search for sra homologs in other organisms in the nucleotide databases revealed the presence of homologous genes in Salmonella serovar Typhimurium and three other enterobacteria (Fig. 7). However, no homologous genes could be found in gram-positive bacteria such as Bacillus subtilis, in spite of extensive searches with different search options (K. Isono and N. Ogasawara, personal communication). This result suggests that the sra gene is likely to be specific to gram-negative, most likely enteric, bacteria. Knowing that its copy number increases in the stationary phase, we are interested in establishing the function of SRA in association with ribosomes, particularly in the stationary phase of growth of E. coli and other enteric bacteria. Perhaps SRA plays a role in cells survival under unfavorable environmental conditions upon prolonged culturing.