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Journal of Bacteriology, June 2003, p. 3654-3660, Vol. 185, No. 12
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.12.3654-3660.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Oxidative Stress in Synechococcus sp. Strain PCC 7942: Various Mechanisms for H₂O₂ Detoxification with Different Physiological Roles

Alexander Perelman, Avraham Uzan, Dalia Hacohen, and Rakefet Schwarz^*

Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

Received 2 October 2002/ Accepted 21 March 2003

Top Abstract Introduction Materials and Methods Results Discussion References

 
This study focuses on the mechanisms for hydrogen peroxide detoxification in Synechococcus sp. strain PCC 7942. To gain better understanding of the role of different routes of hydrogen peroxide detoxification, we inactivated tplA (thioredoxin-peroxidase-like), which we recently identified. In addition, we inactivated the gene encoding catalase-peroxidase and examined the ability to detoxify H₂O₂ and to survive oxidative stress in both of the single mutants and in the double mutant. Surprisingly, we observed that the double mutant survived H₂O₂ concentrations that the single catalase-peroxidase mutant could not tolerate. This phenotype correlated with an increased ability of the double mutant to detoxify externally added H₂O₂ compared to the catalase-peroxidase mutant. Therefore, our studies suggested the existence of a hydrogen peroxide detoxification activity in addition to catalase-peroxidase and thioredoxin-peroxidase. The rate of detoxification of externally added H₂O₂ was similar in the wild-type and the TplA mutant cells, suggesting that, under these conditions, catalase-peroxidase activity was essential for this process and TplA was dispensable. However, during excessive radiation, conditions under which the cell might experience oxidative stress, TplA appears to be essential for growth, and cells lacking it cannot compete with the wild-type strain. Overall, these studies suggested different physiological roles for various cellular hydrogen peroxide detoxification mechanisms in Synechococcus sp. strain PCC 7942.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Cells of photosynthetic organisms possess substantial sources of reactive oxygen species (ROS) in addition to the ROS-producing processes common to all living cells. This stems from the need to harvest light energy for their phototrophic metabolism. Accordingly, these organisms face the challenge of capturing light energy efficiently while avoiding oxidative damage caused by a surplus of absorbed light. Excessive excitation stems from an imbalance between energy absorption and dissipation rates (11, 19, 22). Although high photon flux causes excess excitation, light intensity is not the only effective factor. Any environmental parameter that would slow down anabolism (nonoptimal temperature, nutrient limitation, etc.) would decrease photochemical dissipation and therefore may lead to oxidative stress caused by excess absorbed light.

Certain excited pigment molecules may produce ROS, i.e., singlet chlorophyll while decaying through triplet chlorophyll, causes the formation of singlet oxygen (11, 19). The photosynthetic electron transport chain may produce damaging oxygen species as well. For example, production of ROS may occur on the acceptor side of photosystem II through electron flow from phaeophytin or semiquinone. In addition, it is commonly accepted that under excess of absorbed light, photosystem I can reduce molecular oxygen to superoxide anion (Mehler reaction [1]), which can be converted by superoxide dismutase to H₂O₂. Apart from being potentially harmful by itself, in the presence of reduced metal ions H₂O₂ may be converted to hydroxyl radical, a highly reactive and damaging entity. A recent study (13) provided evidence for the involvement of A-type flavoproteins in photoreduction of O₂ by electron transfer from photosystem I (the Mehler reaction) in Synechocystis sp. strain PCC 6803 (referred to here as Synechocystis strain 6803). One of the flavoproteins essential for photoreduction of O₂ has been shown to reduce O₂ directly to water in vitro (35). It has therefore been suggested that in contrast to eukaryotes, the Mehler reaction in cyanobacteria does not produce ROS.

To avoid the damaging consequences of hydrogen peroxide production, cells possess various enzymes that detoxify this compound (9, 11, 21, 24, 27, 32, 33). Hydrogen peroxide-detoxifying enzymes are traditionally classified as catalases or peroxidases (27). Enzymes of the former group convert H₂O₂ to water and molecular oxygen, whereas the peroxidases rely on electrons supplied by reductants of low molecular weight, such as ascorbate and glutathione, to reduce H₂O₂ or organic hydroperoxides. In general, catalases exhibit lower affinity for H₂O₂ and higher k_cat compared to the peroxidases. A third type of hydrogen peroxide-detoxifying enzyme, one unique to prokaryotes, is designated catalase-peroxidase (38). As a typical catalase, this enzyme converts H₂O₂ to water and molecular oxygen but in vitro, it also shows a peroxidase activity with o-dianisidine or pyrogallol as substrates. As suggested by the substrate specificity (23), it is likely that, in vivo, the cyanobacterial catalase-peroxidase functions as a catalase rather than a peroxidase.

An additional antioxidant enzyme that drew attention recently is thioredoxin-peroxidase, a protein conserved from bacteria to mammals. The exact role and mode of regulation of these enzymes are not clear; however, it is established that they rely on reduced thioredoxin (6, 10, 15-18) which in a photosynthetic cell may be reduced by photosystem I through ferredoxin (5).

During studies on acclimation of Synechococcus sp. strain PCC 7942 (referred to here as Synechococcus) to nutrient limitation and high-light stress, we identified an open reading frame (ORF) that is highly homologous to thioredoxin-peroxidases (tplA, for thioredoxin-peroxidase-like). To obtain a comprehensive picture of the relevance of TplA, as well as of the catalase-peroxidase, for hydrogen peroxide detoxification and cell survival, we inactivated the genes encoding for these enzymes and characterized each of the single mutants, as well as the double mutant.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Culture conditions and competition experiments. Synechococcus and its mutants were cultured as previously described (7). For growth experiments under high-light conditions the cultures were illuminated with 800 µmol of photons m^-2 s^-1 (provided by halogen lamps cooled with water jackets) and bubbled with air. The same conditions were applied for "competition tests" in which a mixture of equal amounts of wild type and a certain mutant (optical density at 750 nm [OD₇₅₀] of 0.02) served to initiate the experiment. The mixed culture was diluted daily to the original OD; thus, the absorbed light remained fairly constant throughout the experiment. The generation time under these conditions was about 12 h. The number of wild-type and mutant cells in the culture at a given time was assessed by diluting and plating the cultures on solid medium, followed by restreaking of single colonies on plates containing the appropriate antibiotic for the selection of a specific mutant.

Construction of mutant strains, DNA manipulation, and isolation of RNA. It is often observed that genes, the products of which are involved in cell growth and survival under certain environmental conditions, are clustered. Therefore, we sequenced further downstream of nblR, the gene encoding for a response regulator that is essential for cell survival during high-light illumination and nutrient starvation (30). This led to identification of an ORF highly homologous to thioredoxin-peroxidases located ca. 2 kb downstream of nblR. A 2-kb EcoRV/EcoRI subclone was used for interposon inactivation of tplA by insertion of a spectinomycin resistance cassette at an XcmI site.

The gene encoding for catalase-peroxidase of Synechococcus, katG, was amplified from genomic DNA by using primers designed according to the published sequences (23) (5'-CCAAACACCAACAGGAGA-3' and 5'-GTTGCGATAGCATCGTGA-3'). The PCR product was cloned into a pGEM-T vector (Promega). Digestion by ClaI was used to delete a 609-bp fragment, which was replaced by a kanamycin resistance cassette. Each of the plasmids containing either the inactivated tplA or katG was used to transform the wild-type strain to yield the tplA mutant (Tpl) and the katG mutant (Kat), respectively.

The double mutant, KatTpl, characterized throughout the present study was obtained by transforming the plasmid bearing the inactivated katG into Tpl. Where indicated, a double mutant constructed by transforming Kat with inactivated tplA was analyzed. PCR on genomic DNA isolated from the transformants by using specific primers confirmed the complete segregation and replacement of the native gene with the inactivated one.

Molecular techniques were performed according to standard procedures (29). For transcript analyses, cultures were illuminated with 70 or 400 µmol of photons m^-2 s^-1 provided by fluorescent lamps. RNA isolation and Northern blot analyses were performed as described earlier (8). The 0.5-kbp EcoRI/XmnI fragment containing part of tplA was used as a probe.

Determination of viability and analysis of H₂O₂ detoxification by whole cells. To determine viability after H₂O₂ treatment, cells at the exponential growth phase were adjusted to an OD₇₅₀ of 0.5, and H₂O₂ was applied to a range of final concentrations (15 µM to 10 mM, depending on the specific strain). After incubation for 24 h under incandescent light (50 µmol of photons m^-2 s^-1), aliquots were placed onto solid medium plates to assess viability.

For analysis of H₂O₂ detoxification, cells harvested during exponential growth (5,000 x g, 5 min) were washed once with an equal volume of 20 mM NaNO₃, 0.3 mM MgSO₄ · 7H₂O, and 0.2 mM CaCl₂ · 2H₂O (the concentrations of these salts in the growth medium) and resuspended in the same solution to an OD₇₅₀ of 0.5. H₂O₂ was then added to the desired final concentrations. Aliquots were drawn at various times and diluted when required, and the amount of H₂O₂ was determined by oxidation of Fe2+ in the presence of xylenol orange (36) using a FOX assay (36) by measuring the absorbance with a Multiskan RC (Labsystems) with a 560-nm band-pass filter. The initial slope of the absorbance as a function of time served to quantify the amount of H₂O₂. Qualitative determination of H₂O₂ was obtained by carrying out color development to its maximal level. In these assays, yellow color represents the absence and purple represents the presence of H₂O₂.

Where indicated, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was added to a final concentration of 10 µM. Green light (50 µmol of photons m^-2 s^-1) was provided with a 520-nm filter.

As previously shown for Synechocystis strain 6803 (34), we noticed that the ability to decompose H₂O₂ was strongly dependent on cell density. Therefore, all of the analyses presented in the present study were performed at a fixed cell density. Changing the cell density, however, affected the absolute H₂O₂ concentrations that the strains could tolerate and detoxify but not the relative differences between the mutants.

The nucleotide sequence of the genomic region containing tplA was deposited in GenBank under accession no. AF492495.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Identification of a thioredoxin-peroxidase-like gene. During studies of the acclimation of Synechococcus to nutrient limitation and high-light stress, we have identified an ORF highly homologous to thioredoxin-peroxidases (also termed peroxiredoxins), enzymes conserved from microorganisms to mammals. For example, the Synechococcus TplA exhibits 88% similarity to sll0755 of Synechocystis strain 6803 (http://www.kazusa.or.jp/cyanobase/), 82% similarity to BAS1 from barley (2), 75% similarity to human thioredoxin-peroxidase (31), and 55% similarity to AhpC from Escherichia coli (4). The highest homology was observed between TplA and a subfamily of thioredoxin-peroxidases designated 2-Cys peroxiredoxins. These enzymes function as homodimers in which a disulfide bridge between two conserved cysteins is formed during catalysis (6). Sequence alignment indicated that TplA possesses the two conserved cystein residues essential for catalysis (not shown). Although the physiological role of these enzymes is not completely understood, it has been established that peroxiredoxins reduce hydrogen peroxide or alkyl hydroperoxides by using electrons from thioredoxin (10, 12, 14, 25).

Viability of wild-type and mutant strains after H₂O₂ treatment. To study the physiological role of TplA in Synechococcus, we inactivated its gene and characterized the mutant with respect to its ability to survive externally added H₂O₂, to detoxify H₂O₂, and to grow during high-light illumination, conditions under which the cells might experience oxidative stress. In order to gain better understanding of the ability of the cell to cope with oxidative stress, we also inactivated the gene encoding for catalase-peroxidase and characterized this mutant, as well as the double mutant lacking both genes.

The various strains were exposed to H₂O₂ in liquid cultures for 24 h, followed by transfer of aliquots onto solid growth medium (Fig. 1). Strains possessing catalase-peroxidase (wild type and Tpl) survived exposure to 50- to 100-fold-higher concentrations of H₂O₂ than strains lacking this activity (Kat and KatTpl) (Fig. 1A and C, respectively). Apparently, catalase-peroxidase is required for detoxification of high concentrations of externally added H₂O₂.

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FIG. 1. Cells spotted on solid growth medium (A and C) and qualitative assay for remaining H2O2 (B and D) after incubation for 24 h with the indicated concentrations of H2O2. Yellow represents the absence and purple represents the presence of H2O2. WT, wild type; Tpl, tplA mutant; Kat, catalase-peroxidase mutant; KatTpl, double mutant. In the specific experiment shown, viability and residual H2O2 were determined simultaneously, and thus cells were prepared as described for the determination of H2O2 content (see Materials and Methods).

The ability to detoxify H₂O₂ likely contributes to cell survival during oxidative stress and, therefore, we expected the double mutant to be the most sensitive to the application of H₂O₂. Surprisingly, the double mutant survived higher H₂O₂ concentrations than did the catalase-peroxidase mutant (Fig. 1C). Since the mutants were fully segregated (see Materials and Methods), the data suggested that inactivation of both katG and tplA resulted in the induction of H₂O₂ detoxification activity that is supplementary to catalase-peroxidase and thioredoxin-peroxidase.

Detoxification of H₂O₂ by wild-type and mutant cultures. Qualitative determination of the residual H₂O₂ remaining after 24 h of incubation was performed on the cultures that served to assess the viability (Fig. 1). These analyses showed that the higher ability of the double mutant to survive H₂O₂ (compared to the catalase-peroxidase mutant) coincided with its increased capacity to detoxify these levels of H₂O₂ (Fig. 1C and D). These experiments also indicated that catalase-possessing strains were able to detoxify 100- to 200-fold-higher concentrations of H₂O₂ than catalase-lacking strains (Fig. 1B and D). It is interesting that in catalase-lacking strains (Kat and KatTpl), cell survival under a certain concentration of H₂O₂ correlated with the ability to decompose it (Fig. 1C and D). On the other hand, catalase-possessing strains (wild type and Tpl) could detoxify H₂O₂ concentrations that caused cell death (Fig. 1A and B). Presumably, KatG remains active in H₂O₂-damaged cells, whereas peroxidases can no longer function if their electron source is exhausted.

The contribution of the different enzymes to H₂O₂ detoxification was assessed by monitoring the rate of H₂O₂ decomposition by the various strains. Comparisons between the rates of detoxification by illuminated and darkened cultures were performed since photosynthetic electron transport may be essential for replenishment of the reducing equivalents required for peroxidase activity.

The Kat and the double mutant decomposed relatively low concentrations of H₂O₂ (15 or 30 µM) at a similar rate under either illumination or darkness (Fig. 2A and B). Challenging these strains with higher H₂O₂ levels (50 and 100 µM) revealed a substantial difference between these mutants (Fig. 2C and D). The KatTpl mutant completely eliminated 50 µM within 12 min of incubation in the light and was capable of partially reducing even 100 µM H₂O₂ within 40 min of illumination, unlike Kat, which did not reduce such concentrations (Fig. 2C and D). These experiments confirmed our finding (Fig. 1) that the ability of the double mutant to survive high H₂O₂ levels originated from its ability to detoxify them.

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FIG. 2. Time courses of decomposition of H2O2 by illuminated (solid lines) or darkened (dashed lines) cultures of catalase-peroxidase mutant () or the double mutant KatTpl (). At time zero, 15 µM (A), 30 µM (B), 50 µM (C), or 100 µM (D) H2O2 was added to the cultures.

In the dark, incubation with H₂O₂ did not reveal a difference between Kat and the double mutant; both strains were unable to reduce either 50 or 100 µM H₂O₂ (Fig. 2C and D). These two mutants, however, detoxified lower H₂O₂ concentrations similarly, although at a slower rate compared to cultures incubated in the light. The dependence of H₂O₂ detoxification in these strains on light suggests a requirement for reductants produced by the photosynthetic electron transport chain. This was further supported by the lack of light-dependent H₂O₂ detoxification upon addition of DCMU or illumination with a nonphotosynthetic (green) light (not shown). Therefore, reduction of relatively low concentrations of H₂O₂ in the dark (Fig. 2A and B) may depend on the availability of cellular pools of reduced compounds.

In accordance with their ability to survive high levels of H₂O₂, wild type and Tpl were capable of detoxifying relatively high concentrations of H₂O₂ compared to Kat and the double mutant; 1 and 4 mM H₂O₂ were completely decomposed within 15 and 65 min, respectively. High concentrations of H₂O₂, such as 7 and 10 mM, were only partially reduced within 65 min (Fig. 3), presumably due to accumulating damage to enzyme activities. In addition, the rate of detoxification of H₂O₂ in the wild type and the tplA mutant were essentially identical (Fig. 3), suggesting that catalase activity was not affected by the inactivation of tplA.

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FIG. 3. Time courses of decomposition of H2O2 by illuminated cultures of wild type (A) and tplA mutant (B). At zero time, 1 mM (), 4 mM (), 7 mM (), and 10 mM () H2O2 was added to the cultures. Identical curves were obtained with darkened cultures.

Identical rates of H₂O₂ detoxification were observed in illuminated (Fig. 3) or darkened cultures (not shown) of the wild-type and Tpl cells. Since peroxidases but not catalases rely on reduced constituents originating from photosynthetic electron transfer, this result indicates that the catalase function of the catalase-peroxidase provides the dominant activity required for detoxification of the high H₂O₂ levels. Catalase also appears to be the principal means for decomposition of lower concentration of H₂O₂; Tpl detoxified 5 µM H₂O₂ much faster than did Kat (not shown).

Response of mutant strains to high-light conditions during growth. In addition to detoxification of externally supplied H₂O₂ (Fig. 1 to 3), it was important to assess the ability of the various strains to cope with environmental conditions that might result in oxidative stress, such as growth under intensive radiation. Mutants where tplA was inactivated (Tpl and KatTpl) grew very poorly under these conditions, whereas Kat grew similarly to the wild-type cells (Fig. 4). Furthermore, growth experiments performed under high-light irradiance demonstrated that Tpl and the double mutant were outcompeted by the wild type, specifically under these conditions (Fig. 5), but not when the light intensity was 100 µmol of photons m^-2 s^-1 (not shown). Taken together, these data may indicate a crucial role for TplA under a high-light regime. Furthermore, the novel H₂O₂ detoxification activity observed in the double mutant cannot compensate for the lack of TplA under these conditions. An additional support for the importance of TplA for high-light growth was provided by the fivefold rise in the abundance of the tplA transcript (Fig. 6) after the high-light treatment.

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FIG. 4. Growth as measured by the change in OD750 of wild type (), Kat (), Tpl (), and the double mutant KatTpl () during illumination with high-light intensity.

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FIG. 5. Percentage of CFU of wild type (shaded), Kat (hatched), Tpl (dotted), and the double mutant KatTpl (check pattern) at time zero and after 5 days of growth in a mixed culture illuminated with 800 µmol of photons m-2 s-1 (see Materials and Methods for details of the competition tests).

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FIG. 6. Northern blot hybridization with a tplA specific probe to RNA isolated from Synechococcus grown at 70 µmol of photons m-2 s-1 (lane 1) or exposed to 400 µmol of photons m-2 s-1 for 45 min (lane 2) or 2 h (lane 3). The probe hybridized to a single band of ca. 800 bp. Each lane was loaded with 5 µg of RNA.

Top Abstract Introduction Materials and Methods Results Discussion References

 
A novel H₂O₂ detoxification activity in Synechococcus. The double mutant, KatTpl, survived and detoxified H₂O₂ concentrations that were not tolerated or reduced by the Kat (Fig. 1 and 2). These observations indicated a novel H₂O₂ detoxifying activity, in addition to that of TplA and catalase-peroxidase. Presumably, the supplementary H₂O₂ detoxification activity is expressed in the absence of both KatG and TplA. This novel activity relies on reductants produced by the photosynthetic electron transport chain, as suggested by its dependence on photosynthetic light and inhibition by DCMU. It is plausible that inactivation of both tplA and katG caused the induction of the novel H₂O₂ detoxification activity. An alternative possibility is that a spontaneous mutation present in Tpl contributed to the phenotype of the double mutant since the latter was raised by inactivation of katG in Tpl. However, a TplKat mutant that was raised by inactivation of tplA in Kat also exhibited higher resistance to H₂O₂ compared to Kat (not shown), thus supporting the hypothesis that the lack of both KatG and TplA causes the induction of a supplementary H₂O₂-decomposing activity.

Interestingly, the activity observed in the katG mutant (most likely originating from TplA) is more susceptible to H₂O₂ treatment than is the activity exhibited by the double mutant. For example, the lack of detoxification of 50 or 100 µM H₂O₂ by the katG mutant implies rapid oxidation of the reduced substrates required for H₂O₂ detoxification and/or damage to the enzyme itself. On the other hand, the activity present in the double mutant was not impaired under these conditions (Fig. 2), although the addition of 200 µM of H₂O₂ eliminated this activity as well (not shown).

Although originally classified as lacking ascorbate peroxidase (21), Synechococcus was shown to possess an ascorbate peroxidase-like activity (28). This activity, which appeared to be cytosolic, copurified with a small non-heme iron-containing compound and was heat stable. This ascorbate-peroxidase-like activity probably does not account for the H₂O₂-detoxifying activity observed in the double mutant since initial characterization of this activity indicated that it is membrane associated and heat inactivated, unlike the ascorbate-dependent activity. Currently, we are characterizing the novel H₂O₂-detoxifying activity observed in the double mutant to clarify its nature and physiological significance.

Differential role for catalase-peroxidase and TplA. Analyses of viability (Fig. 1) and of H₂O₂ detoxification (Fig. 1 to 3) indicated that catalase-peroxidase is essential for survival and the elimination of relatively high concentrations of externally added H₂O₂. Studies of Synechocystis strain 6803 and its katG mutant also suggested such a role (34). Despite its importance for the elimination of relatively high concentrations of H₂O₂, catalase-peroxidase seems to be dispensable for growth under high-light illumination (Fig. 4 and 5), conditions under which the cell might experience severe oxidative stress (26). Further, although it reduced externally added H₂O₂ as efficiently as the wild type (Fig. 3), a mutant lacking TplA grew very poorly and was outcompeted by wild-type cells (Fig. 4 and 5, respectively) during excessive radiation. Under the latter conditions, the double mutant exhibited a phenotype similar to that of Tpl (Fig. 4 and 5). This indicated that TplA was crucial for growth under high-light conditions and the supplementary H₂O₂-detoxifying activity observed in the double mutant could not replace it. Evidence for the importance of thioredoxin-peroxidase for high-light growth is also provided by the effect of high-light treatment on the quantum yield in Synechocystis strain 6803 and its thioredoxin-peroxidase mutant; the latter exhibited a lower quantum yield compared to wild-type cells (18). In addition to the high-light-sensitive phenotype of the TplA-lacking mutants (Fig. 4 and 5), transcription induction of tplA observed in wild-type cells, following high-light illumination (Fig. 6), also supports the physiological relevance of this gene product under high-light conditions. The tplA transcript appears to be monocistronic (the probe hybridized to a 800-bp transcript), and therefore it is likely that the phenotype is not a result of a polar effect.

As is apparent from our study, neither catalase-peroxidase nor the additional peroxidase activity present in the double mutant could substitute for TplA function during high-light growth. Given that the photosynthetic electron transport chain reduces thioredoxin, one may speculate that a thioredoxin-dependent peroxidase provides an energy dissipation route, which might be critical under excessive excitation. Support for this hypothesis comes from studies on Synechococcus by Miller et al. (20), which showed photosystem II-dependent oxygen evolution and fluorescence quenching consequent on photoreduction of H₂O₂. Further support is provided by experiments on Synechocystis strain 6803 showing that when linear electron flow is blocked, the addition of t-butyl-hydroperoxide enabled oxygen evolution by wild type but not by a thioredoxin-peroxidase mutant (37). Production of organic hydroperoxides during excessive radiation and their specific reduction by TplA may provide an additional explanation for the indispensable role of this enzyme.

It is important to point out that the Arabidopsis genome contains 10 ORFs showing homology to members of the peroxiredoxins (thioredoxin-peroxidases) family (10). Use of Arabidopsis plants expressing antisense to the chloroplast-located thioredoxin-peroxidase suggested a protective function for the enzyme (3). However, the exact role and physiological significance of these enzymes and that of peroxiredoxins identified in other plants is yet to be established. Sequences showing homology to catalase-peroxidase were not identified in the complete genomes of Anabaena sp. strain PCC 7120, Nostoc punctiforme ATCC 29133, Synechococcus strain WH 8102, and two Prochlorococcus marinus strains (MI9313 and MED4). In fact, the latter three marine organisms do not possess sequences that show homology to any catalase (mono-functional heme or manganese enzyme). On the other hand, each of the above-mentioned organisms possesses at least one sequence that is highly homologous to thioredoxin-peroxidase. These observations further emphasize the physiological significance of thioredoxin-peroxidases.

 
A.P. and A.U. contributed equally to this study.

This work was supported by United States-Israel Binational Science Foundation (grant 9800146) and by The Israel Science Foundation (grant 210/99).

 
* Corresponding author. Mailing address: Faculty of Life Sciences, Bar-Ilan University, 52900 Ramat-Gan, Israel. Phone: (972) 3-5317790. Fax: (972) 3-5351824. E-mail: schwarr2{at}mail.biu.ac.il.

Top Abstract Introduction Materials and Methods Results Discussion References

 

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Journal of Bacteriology, June 2003, p. 3654-3660, Vol. 185, No. 12
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.12.3654-3660.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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