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Journal of Bacteriology, September 2004, p. 5614-5620, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5614-5620.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

A Regulatory Trade-Off as a Source of Strain Variation in the Species Escherichia coli

Thea King,¹ Akira Ishihama,² Ayako Kori,² and Thomas Ferenci^1*

School of Molecular and Microbial Biosciences G08, The University of Sydney, Sydney, Australia,¹ Nippon Institute for Biological Science, Ome, Tokyo, Japan²

Received 30 March 2004/ Accepted 18 May 2004

Top Abstract Introduction Materials and Methods Results Discussion References

 
There are few existing indications that strain variation in prokaryotic gene regulation is common or has evolutionary advantage. In this study, we report on isolates of Escherichia coli with distinct ratios of sigma factors (RpoD, ^D, or ⁷⁰ and RpoS or ^S) that affect transcription initiated by RNA polymerase. Both laboratory E. coli K-12 lineages and nondomesticated isolates exhibit strain-specific endogenous levels of RpoS protein. We demonstrate that variation in genome usage underpins intraspecific variability in transcription patterns, resistance to external stresses, and the choice of beneficial mutations under nutrient limitation. Most unexpectedly, RpoS also controlled strain variation with respect to the metabolic capability of bacteria with more than a dozen carbon sources. Strains with higher ^S levels were more resistant to external stress but metabolized fewer substrates and poorly competed for low concentrations of nutrients. On the other hand, strains with lower ^S levels had broader nutritional capabilities and better competitive ability with low nutrient concentrations but low resistance to external stress. In other words, RpoS influenced both r and K strategist functions of bacteria simultaneously. The evolutionary principle driving strain variation is proposed to be a conceptually novel trade-off that we term SPANC (for "self-preservation and nutritional competence"). The availability of multiple SPANC settings potentially broadens the niche occupied by a species consisting of individuals with narrow specialization and reveals an evolutionary advantage offered by polymorphic regulation. Regulatory diversity is likely to be a significant contributor to complexity in a bacterial world in which multiple sigma factors are a universal feature.

Top Abstract Introduction Materials and Methods Results Discussion References

 
The major source of variation in prokaryotes is thought to be the loss or gain of functional genes or elements, such as pathogenicity islands (14, 33). Members of a bacterial species such as Escherichia coli have common properties and similar chromosomal organizations, but the species is phenotypically diverse (44). Isolates of E. coli exhibit many distinct properties, including distinct growth rates (28) and stress sensitivities (1, 43). Some of the differences are undoubtedly due to loss or gain of genes, but is there also a difference in gene usage or expression between strains? The gene regulatory consistency of bacteria is relatively poorly studied, but it needs to be understood if the full range of bacterial variation is to be established. In this study, we investigated whether strain-specific gene usage is a source of bacterial variation in E. coli.

Our starting point for examining this question arose from recent studies of the polymorphism of the RpoS sigma factor in isolates of E. coli and Salmonella (11, 31). If a central regulator of stress resistance genes (RpoS or ^S [24, 40]) is not conserved, then how constant is gene usage on a global scale? It is evident from both laboratory studies and the occurrence of rpoS mutations in natural populations that regulatory divergence can arise and flourish in particular environments (11). In this study, we found that natural regulatory settings are far from uniform within a species and include a wide range of possibilities.

A significant level of control over expression of multiple genes in bacteria involves RNA polymerase sigma factors, which partition transcription to different bacterial promoters (13, 17). The concentration of a sigma factor, such as ^S, controls general stress resistance, starvation survival (16), and gene expression under nutrient limitation (10). In addition, because ^S competes for a fixed amount of RNA polymerase, the level of ^S also inversely influences the expression of other factor-controlled genes, including housekeeping genes (8, 26). Within this expanding model of cellular control through factor competition (20, 21), we investigated whether RpoS protein levels also influenced additional phenotypic and nutritional abilities of various E. coli strains. As shown below, an unexpected inverse relationship between stress resistance and nutritional capabilities was found in different strains. Furthermore, a molecular explanation of strain variation can now be offered on the basis of the equally unexpected variation in the endogenous concentration of sigma factors within a species. The numerous implications of these findings for understanding bacterial diversity and evolution are discussed below.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Strains and strain construction. All bacterial strains used in this study are shown in Table 1. P1 transduction (29) with P1 cml clr1000 grown on ZK1171 was used to introduce rpoS::Tn10 into BW2952 and MG1655. lac⁺ derivatives of BW2952, BW3709, ZK126, and ZK1171 were made by P1 transduction with P1 cml clr1000 grown on MG1655.

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TABLE 1. Strains used in this study

To study nondomesticated E. coli strains, the extensive collection of P. Reeves (Sydney, Australia) was surveyed for rpoS-related properties. Forty-one pathogenic and EcoR isolates were screened (34). Of these, only 16 strains were RpoS⁺ as determined by the glycogen screening test described below. In further phenotypic screening, isolates EcoR38 and EcoR10 and O157:H7 isolate M534 were found to exhibit the range of properties shown by K-12 strains MG1655, ZK126, and BW2952 and were used for further experiments. An rpoS mutation could not be introduced into the P1-resistant non-K-12 strains by transduction, so rpoS null mutants of M534 and EcoR38 were isolated directly from chemostat cultures as previously described (31) to obtain strains BW3737 and BW3736, respectively.

Growth medium and culture conditions. The medium used in chemostat cultures was minimal medium A (29). The carbon source in all cases was glucose, which was present at a concentration of 0.02 or 0.04% (wt/vol) in the feed medium in glucose-limiting experiments. For batch cultures and agar plates, glucose or acetate was included at a concentration of 0.2% (wt/vol). Eighty-milliliter chemostat cultures were set up as described previously (31). The dilution rates were set to 0.1 h^–1 (doubling time, 6.9 h). The culture densities were between 1.9 x 10⁸ and 2.1 x 10⁸ bacteria ml^–1.

To assess the metabolism of 95 substrates by the strains in a Biolog GN2 MicroPlate (Oxoid Ltd., Sydney, Australia) (3), the manufacturer's instructions were followed. Positive readings were defined as optical densities at 600 nm of >0.2 after 24 h of incubation.

Detection of rpoS status. rpoS mutants were distinguished from wild-type strains by staining glycogen in colonies on Luria agar plates. The plates were incubated overnight at 37°C and then left at 4°C for 24 h before they were flooded with concentrated iodine as previously described (31).

rpoS amplification and DNA sequencing. A 1,302-bp fragment containing the rpoS gene was amplified from chemostat isolates by PCR by using two external primers, RpoSF1 (5'-CGGACCTTTTATTGTGCACA-3') and RpoSR1 (5'-TGATTACCTGAGTGCCTACG-3'), and an internal primer, RpoSI (5'-CTGTTAACGGCCGAAGAAGA-3'), as previously described (31).

ß-Galactosidase and catalase assays. Five-milliliter samples were removed from chemostat cultures, and ß-galactosidase activity was measured as described by Miller (29) by using sodium dodecyl sulfate and chloroform-treated cells. KatE/hydroperoxidase II catalase activity was assayed as described by Visick and Clarke (42).

Quantitation of RNA polymerase subunits. Bacteria were harvested from 1-day-old chemostats, extracted, and analyzed by using the standard quantitative immunoblot system (19). Probing was performed with antibodies against purified RpoA, RpoD, or RpoS in parallel with known amounts of purified RNA polymerase subunits. The data presented below are means from three blots of each of two independent samples.

Tolerance to external stress. Assays were conducted with 1-day-old chemostat cultures (31) of each strain. To test acid resistance in rich media, the percentage of survivors was measured after 30 min of exposure to Luria broth acidified to pH 1 with HCl. Bacteria were plated directly onto nutrient agar plates, and dilutions were counted after overnight incubation at 37°C. Survival of bacteria in water was assessed after 15 h of incubation at 25°C.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Strain variation in metabolism and stress resistance. We compared six E. coli strains, all rpoS⁺, for metabolism of 95 substrates in a Biolog assay (3). Several strains utilized 47 to 50 substrates, but BW2952 and M534 metabolized only 31 and 24 substrates, respectively (see Table S2 in the supplemental material). To test the possible role of ^S in metabolism, rpoS-defective derivatives of the strains were also assayed. Strikingly, the number of substrates metabolized by M534 and BW2952 greatly increased upon introduction of an rpoS mutation (Fig. 1A). The nutritional profiles of the rpoS disruption mutants were generally similar. Some individual metabolic differences were found and were probably due to structural gene differences between strains (35), but the results in Fig. 1 suggest that RpoS has a pleiotropic effect on the metabolic capability of certain bacteria. The substrates that were poorly utilized by both BW2952 and M534, whose metabolism was stimulated by an rpoS disruption, included D-melibiose, ß-methyl-D-glucoside, L-rhamnose, D-sorbitol, acetic acid, D-galacturonic acid, succinic acid, bromosuccinic acid, L-alanine, L-alanyl-glycine, L-asparagine, L-aspartic acid, and DL--glycerol phosphate. The complete Biolog results are shown in Table S2 in the supplemental material.

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FIG. 1. Strain variation in substrate utilization and the role of RpoS. (A) Numbers of carbon sources metabolized by the strains. A total of 95 substrates were examined with a Biolog GN2 MicroPlate (Oxoid Ltd.) (3). (B) Resistance to exposure to pH 1 for 30 min. (C) Starvation survival after 15 h of incubation in water at 25°C. Assays were conducted with 1-day-old chemostat (31) samples of each strain, and survival was measured by determining viable counts. The error bars indicate the standard deviations based on two replicate experiments. The rpoS status of each strain is indicated by a plus sign or a minus sign. The designations of the rpoS derivatives of the parental strains are as follows: BW3737 (M534), BW3709 (BW2952), ZK1171 (ZK126), BW3736 (EcoR38), and BW3708 (MG1655).

Interestingly, the metabolic capabilities were inversely related to the stress resistance properties of the six strains. Consistent with previous surveys, E. coli isolates are not uniformly stress resistant (1, 43) and as shown in Fig. 1B and C, the nutritionally versatile strains, such as MG1655 and EcoR10, were the strains that were most sensitive to stress. Conversely, the nutritionally restricted strains were the most stress resistant. An rpoS mutation disrupted resistance to starvation and the osmotic shock that would be experienced during incubation in water, as expected from the established role of RpoS (16). Similarly, resistance to acid was also low in rpoS mutants.

Acetate was one of the substrates whose metabolism was stimulated by an rpoS disruption. A further indication of the role of ^S in nutrition came from prolonged incubation of the E. coli K-12 isolates on acetate plates (Fig. 2). BW2952 showed much poorer growth than MG1655, which is consistent with the Biolog data. Growth of ZK126 was partially impaired on acetate plates. However, after 5 days, individual colonies that grew faster appeared in the BW2952 streak lines on acetate medium. All of these colonies proved to be rpoS mutants (data not shown). Growth of a defined rpoS derivative of the BW2952 strain, as well as ZK126 (Fig. 2), on acetate was much faster, so the suppression of metabolic capacity by RpoS could be overcome by rpoS mutations.

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FIG. 2. Strain variation in the growth of E. coli K-12 with acetate as the sole carbon source. The rpoS status of each E. coli K-12 strain is indicated by a plus sign or a minus sign. The designations of the rpoS derivatives of the parental strains are as follows: BW3709 (BW2952), ZK1171 (ZK126), and BW3708 (MG1655). The RpoS/RpoD protein ratio in acetate-grown bacteria is indicated for each of the rpoS+ strains; the ratios for the rpoS strains were less 0.03. For quantitation of RpoS and RpoD levels in acetate cultures we used the standard quantitative immunoblot system (19).

Sigma factor levels in strains of E. coli. To test the basis of the differences in metabolic and stress properties among the RpoS⁺ strains, the endogenous levels of the RNA polymerase components and factors (40) were measured in the strains, as shown in Fig. 2 and 3. In quantitating the concentration of the ^S factor relative to the concentration of a core subunit (RpoA) or the housekeeping-metabolic factor (RpoD), it was clear that the RpoD/RpoA ratio was relatively constant (Fig. 3). In contrast, the amount of ^S varied, and the organisms with a low RpoS/RpoD ratio were more proficient in acetate utilization and metabolism generally. Unexpectedly, the three K-12 strains shown in Fig. 2 differed in the proportion of the sigma factor over a sixfold range during growth on acetate despite having identical rpoS sequences (results not shown). The difference in RpoS levels was also not confined to acetate medium, and the concentrations of RpoS protein were markedly different in isolates at identical steady-state growth rates in a glucose-limited chemostat (Fig. 3). Especially interesting was the relationship among stress sensitivity, metabolic capacity, and the endogenous level of RpoS.

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FIG. 3. Strain variation in levels of sigma factors. (A) Quantitation of RpoD relative to core subunit RpoA. (B and C) RpoS/RpoA (B) and RpoS/RpoD (C) ratios of 1-day chemostat samples determined as described by Jishage and Ishihama (19) by using antibodies against purified RpoA, RpoD, or RpoS in parallel with known amounts of purified RNA polymerase subunits. Error bars represent the standard deviations from three blots of each of two independent samples.

Transcriptional effects of distinct RpoS/RpoD ratios. The most likely way that RpoS levels influenced metabolic and stress capabilities was through altered patterns of transcription. The effect of having distinct steady-state RpoS levels in the six isolates was revealed by comparing the expression of housekeeping genes transcribed by using RpoD (^D or ⁷⁰) with the expression of genes expressed through RpoS or ^S (Fig. 4). Consistent with the ^S/ ^D ratios in Fig. 3, quantitation of expression of a ^D-dependent gene, lacZ, showed that there was a trend towards increasing lacZ expression with decreasing ^S in strains, and the highest levels of LacZ were in rpoS mutants (Fig. 4A). Conversely, when katE, an rpoS-dependent gene (30), was examined, the levels of expression were highest in the high RpoS strains (Fig. 4B) (EcoR38 was anomalous in not having KatE activity). There was a good correlation between the expression patterns and the stress and metabolism capabilities of the six strains.

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FIG. 4. Strain variation in gene expression. (A) Expression of lacZ as determined by quantitating ß-galactosidase activity. (B) Specific activity of KatE/hydroperoxidase II (42) of chemostat samples of each strain. The rpoS status of each strain is indicated by a plus sign or a minus sign. The designations of the rpoS derivatives of the parental strains are as follows: BW3737 (M534), BW3709 (BW2952), ZK1171 (ZK126), BW3736 (EcoR38), and BW3708 (MG1655). The error bars indicate the standard deviations based on two replicates.

Strain variation in mutational adaptation and competitive ability. RpoS levels in different strains of E. coli influenced two other bacterial characteristics. First, the mutational adaptation pathway of strains growing under nutrient limitation (10) was initiated differently. Under experimental evolution conditions (32), as shown in Fig. 5, some strains, including strain BW2952 studied previously (11, 31), rapidly accumulated rpoS mutations in chemostats under glucose limitation. ZK126 accumulated rpoS mutations more slowly, whereas populations of MG1655 did not acquire rpoS mutations. Again, there was a good correlation between RpoS and ^S-dependent transcriptional patterns and the rate of mutation accumulation; the strains with high ^S levels were under stronger pressure to lose RpoS in a nutrient-stressed situation. These results parallel the acetate mutation selection results shown in Fig. 2.

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FIG. 5. Selection for loss of RpoS in glucose-limited populations: appearance of rpoS mutations in chemostat populations provided with 0.02% glucose at a dilution rate of 0.1 h–1. The proportion of mutants was measured as described by Notley-McRobb et al. (31). The error bars indicate standard deviations based on counts from two or three replicate populations.

An important ecological characteristic of bacteria is the ability to compete for low levels of nutrients (9). As shown in Fig. 6, the RpoS status is a major determinant of fitness in a low-nutrient environment. The BW2952 strain with a high level of ^S was initially outcompeted in a glucose-limited environment compared to MG1655 (Fig. 6A), so not only was the BW2952 strain more restricted in terms of nutritional range, but it also had a lower fitness for glucose. After further growth, the appearance of rpoS derivatives in the BW2952 subpopulation increased the competitiveness of the clone, whereas no rpoS mutants of the MG1655 bacteria appeared. The proportion of the BW2952 clone continued to increase due to the accumulation of further mutations described elsewhere (32). When competition experiments were started with rpoS derivatives of BW2952 and MG1655, there was no initial difference in fitness, suggesting that the two strains had similar metabolic potentials once the constraint imposed by RpoS was removed (Fig. 6).

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FIG. 6. Role of RpoS in competition for low nutrient concentrations. (A) Chemostat cultures operating at a dilution rate of 0.1 h–1 with 0.04% glucose in the feed medium were inoculated with equal proportions of 1-day chemostat-grown cultures of either MG1655 (rpoS+) () and BW2952 (rpoS+) (•) or MG1655 (rpoS) () and BW2952 (rpoS) (). The proportion of each strain in the population was determined by plating the appropriate dilution onto Luria agar and selective media. (B) Appearance of rpoS mutations in BW2952 in the experiment whose results are shown in panel A. The proportion of RpoS+ cells in the population was determined by staining with iodine. The error bars indicate the standard deviations for three replicate counts.

Top Abstract Introduction Materials and Methods Results Discussion References

 
The distinct levels of RpoS in different strains were a major source of phenotypic differences in six strains of E. coli. Our results show that even the metabolic profile of bacteria is subject to regulatory variation. This has major implications for microbiology, in which nutrition is often used to type organisms. Our results indicate that the ability to use or not use groups of substrates may be simply a question of global regulation.

Another unexpected conclusion from this study is that a regulatory setting affects both the competitiveness of a bacterium for specific substrates and also its range of substrates. Strains such as EcoR10 and MG1655 are the best specialists for using glucose and also have the broadest nutritional profile. This finding is novel in ecological terms, as generalist and specialist strategies are considered mutually exclusive in ecology (22).

These results also have an impact on our molecular understanding of trade-offs in evolution, which are characterized by the inability of an organism to optimize different traits simultaneously (7, 38). The inverse relationship between nutrition and stress resistance exhibited by bacteria with low and high levels of ^S is not a nutrition-nutrition trade-off like that between R and k strategists (25) or a specialist-generalist balance (22), but it is a novel stress protection-nutrition SPANC ("self-preservation and nutritional competence") trade-off. Our results are also consistent with the conclusion that there is no expected trade-off in fitness between adapting to low concentrations of nutrients and adapting to high concentrations of nutrients (41). Transcriptional competition between factors (8, 26) and the different RpoS/RpoD levels provide a molecular explanation for the set SPANC balance for different isolates.

Historically, it is important that in gene expression studies with E. coli K-12 workers have used numerous genetic backgrounds, including the MG1655, MC4100, and W3110 lineages used here, but our results suggest that RNA polymerase differences need to be considered before strains are interchanged or compared. Indeed, there was a previously noted discrepancy in sigma factor content even within the W3110 lineage (19). It is also relevant that recent results showed that underproduction of RpoD mimics a stringent response (27), which may also partially be the situation in the strains with high ^S levels. In turn, this may be relevant to the finding that growth rate variation is due to differences in ribosomal function (28), which is in turn subject to stringent control (6). Even more intriguingly, the ratios of other sigma factors may also be subject to trade-offs, because the ⁵⁴ content of some W3110 strains was also not constant (19).

From our survey, there is insufficient evidence to suggest that particular ^S levels are associated with particular taxonomic groups or virotypes of E. coli. If anything, the evidence points the other way, with a wide range of settings found even within the taxonomic A subgroup (36), including EcoR10 and the three K-12 strains. Still, a more systematic study is needed to test this point. More speculatively, the variation in factor levels is likely to be variation that can arise frequently, and it occurred independently in the three K-12 lineages, as can happen during prolonged laboratory storage (19, 39). Adaptation of the SPANC balance is therefore likely to be common in nature.

So far, no explanation for what fixes the discrete but distinct RpoS levels in the different strains is available. At least in the three K-12 strains with identical rpoS sequences, the influence on RpoS levels must be extragenic. Complicating matters is the finding that more than one regulatory element may differentiate the strains with low and high RpoS levels because there are numerous, complex inputs for controlling the level of this factor in the cell (15). Several regulators control each stage of rpoS transcription and translation and ^S protein stability (18). Detailed investigation of each input is needed to identify the causes of RpoS variation. Intracellular ppGpp was a potential source of variation in RpoS levels, particularly as BW2952 (an MC4100 derivative) has a known relA1 mutation. However, when ppGpp levels were compared by the method of Rudd et al. (37), there was no correlation between ppGpp levels and RpoS levels. BW2952 had low ppGpp levels but high RpoS levels, whereas M534 had high levels of both. Likewise, the ppGpp level in EcoR10 was lower than the ppGpp level in MG1655, but both strains had low RpoS levels (results not shown). Hence, ppGpp levels are nonuniform in different strains but do not solely explain the RpoS differences observed.

Nevertheless, it is also clear that intragenic changes in rpoS can influence all the properties discussed above. Leaky rpoS mutations that exhibit partial stress resistance are also known to be selected in particular environments (12, 31); these isolates also show altered transcription patterns and partial increases in metabolic versatility (results not shown). The rpoS isolates in population samples (11) also add to the SPANC diversity of bacteria, and rpoS mutants are the best-adapted organisms nutritionally (Fig. 1 and 2). Hence, the SPANC setting of members of E. coli can be adjusted by both extragenic and intragenic rpoS polymorphisms.

In summary, a factor protein that is associated with RNA polymerase and central to global gene expression is present at various endogenous levels in a species. Given that multiple factors are universal in bacteria, it is highly likely that such variations are common in the prokaryotic world and that variation in genome usage extends to bacteria, as well as to higher organisms (4). The regulatory variation resulting from set levels of RpoS provides a means of broadening the ecological and phenotypic properties of a species. These results suggest that polymorphic regulation is central to understanding the phenotypic properties of bacteria, bacterial strain variation, and the trade-offs between environmentally useful characteristics. Finally, the SPANC trade-off may be a more general kind of evolutionary adaptation that may be important for free-living organisms that encounter nonconstant environments. Speculatively, the availability of multiple SPANC settings can be a considerable advantage to a species by broadening its niche, so individuals with narrow SPANC specialization may fill environments with particular stress-nutrition combinations.

 
We thank Etsuko Koshio for some of the assays and Paul Rainey, Mike Cashel, and Andy Holmes for constructive comments.

We also thank the Australian Research Council for funding support.

 
* Corresponding author. Mailing address: School of Molecular and Microbial Biosciences G08, The University of Sydney, Sydney, NSW 2006, Australia. Phone: (61) (2) 9351 4277. Fax: (61) (2) 9351 4571. E-mail: tferenci{at}mail.usyd.edu.au.

Supplemental material for this article may be found at http://jb.asm.org/.

Top Abstract Introduction Materials and Methods Results Discussion References

 

1
1. Benito, A., G. Ventoura, M. Casadei, T. Robinson, and B. Mackey. 1999. Variation in resistance of natural isolates of Escherichia coli O157 to high hydrostatic pressure, mild heat, and other stresses. Appl. Environ. Microbiol. 65:1564-1569.[Abstract/Free Full Text]
2
2. Blattner, F. R., G. Plunkett, C. A. Bloch, N. T. Perna, V. Burland, M. Riley, J. Colladovides, J. D. Glasner, C. K. Rode, G. F. Mayhew, J. Gregor, N. W. Davis, H. A. Kirkpatrick, M. A. Goeden, D. J. Rose, B. Mau, and Y. Shao. 1997. The complete genome sequence of Escherichia coli K-12. Science 277:1453-1462.[Abstract/Free Full Text]
3
3. Bochner, B. R., P. Gadzinski, and E. Panomitros. 2001. Phenotype MicroArrays for high-throughput phenotypic testing and assay of gene function. Genome Res. 11:1246-1255.[Abstract/Free Full Text]
4
4. Carroll, S. B. 2003. Genetics and the making of Homo sapiens. Nature 422:849-857.[CrossRef][Medline]
5
5. Casabadan, M. J. 1976. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and mu. J. Mol. Biol. 104:541-555.[CrossRef][Medline]
6
6. Cashel, M., D. R. Gentry, V. J. Hernandez, and D. Vinella. 1996. The stringent response, p. 1458-1496. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
7
7. Elena, S. F., and R. E. Lenski. 2003. Evolution experiments with microorganisms: the dynamics and genetic bases of adaptation. Nat. Rev. Genet. 4:457-469.[Medline]
8
8. Farewell, A., K. Kvint, and T. Nystrom. 1998. Negative regulation by RpoS—a case of sigma factor competition. Mol. Microbiol. 29:1039-1051.[CrossRef][Medline]
9
9. Ferenci, T. 1996. Adaptation to life at micromolar nutrient levels: the regulation of Escherichia coli glucose transport by endoinduction and cAMP. FEMS Microbiol. Rev. 18:301-317.[CrossRef][Medline]
10
10. Ferenci, T. 1999. Regulation by nutrient limitation. Curr. Opin. Microbiol. 2:208-213.[CrossRef][Medline]
11
11. Ferenci, T. 2003. What is driving the acquisition of mutS and rpoS polymorphisms in Escherichia coli? Trends Microbiol. 11:457-461.[CrossRef][Medline]
12
12. Finkel, S. E., E. R. Zinser, and R. Kolter. 2000. Long-term survival and evolution in the stationary phase, p. 231-238. In Bacterial stress responses. American Society for Microbiology, Washington, D.C.
13
13. Gruber, T. M., and C. A. Gross. 2003. Multiple sigma subunits and the partitioning of bacterial transcription space. Annu. Rev. Microbiol. 57:441-466.[CrossRef][Medline]
14
14. Hacker, J., U. Hentschel, and U. Dobrindt. 2003. Prokaryotic chromosomes and disease. Science 301:790-793.[Abstract/Free Full Text]
15
15. Hengge-Aronis, R. 2002. Signal transduction and regulatory mechanisms involved in control of the ^S (RpoS) subunit of RNA polymerase. Microbiol. Mol. Biol. Rev. 66:373-395.[Abstract/Free Full Text]
16
16. Hengge-Aronis, R. 1993. Survival of hunger and stress: the role of rpoS in early stationary phase gene regulation in E. coli. Cell 72:165-168.[CrossRef][Medline]
17
17. Ishihama, A. 2000. Functional modulation of Escherichia coli RNA polymerase. Annu. Rev. Microbiol. 54:499-518.
18
18. Jenal, U., and R. Hengge-Aronis. 2003. Regulation by proteolysis in bacterial cells. Curr. Opin. Microbiol. 6:163-172.[CrossRef][Medline]
19
19. Jishage, M., and A. Ishihama. 1997. Variation in RNA polymerase sigma subunit composition within different stocks of Escherichia coli W3110. J. Bacteriol. 179:959-963.[Abstract/Free Full Text]
20
20. Jishage, M., K. Kvint, V. Shingler, and T. Nystrom. 2002. Regulation of sigma factor competition by the alarmone ppGpp. Genes Dev. 16:1260-1270.[Abstract/Free Full Text]
21
21. Laurie, A. D., L. M. D. Bernardo, C. C. Sze, E. Skarfstad, A. Szalewska-Palasz, T. Nystrom, and V. Shingler. 2003. The role of the alarmone (p)ppGpp in sigma(N) competition for core RNA polymerase. J. Biol. Chem. 278:1494-1503.[Abstract/Free Full Text]
22
22. Levins, R. 1968. Evolution in changing environments: some theoretical explorations. Princeton University Press, Princeton, N.J.
23
23. Liu, X. Q., and T. Ferenci. 2001. An analysis of multifactorial influences on the transcriptional control of ompF and ompC porin expression under nutrient limitation. Microbiology 147:2981-2989.[Abstract/Free Full Text]
24
24. Loewen, P. C., and R. Hengge-Aronis. 1994. The role of the sigma factor sigma(S) (KatF) in bacterial global regulation. Annu. Rev. Microbiol. 48:53-80.[Medline]
25
25. MacArthur, R. H., and E. O. Wilson. 1967. The theory of island biogeography. Princeton University Press, Princeton, N.J.
26
26. Maeda, H., N. Fujita, and A. Ishihama. 2000. Competition among seven Escherichia coli sigma subunits: relative binding affinities to the core RNA polymerase. Nucleic Acids Res. 28:3497-3503.[Abstract/Free Full Text]
27
27. Magnusson, L. U., T. Nystrom, and A. Farewell. 2003. Underproduction of sigma(70) mimics a stringent response—a proteome approach. J. Biol. Chem. 278:968-973.[Abstract/Free Full Text]
28
28. Mikkola, R., and C. G. Kurland. 1992. Selection of laboratory wild-type phenotype from natural isolates of Escherichia coli in chemostats. Mol. Biol. Evol. 9:394-402.[Abstract]
29
29. Miller, J. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
30
30. Mulvey, M. R., J. Switala, A. Borys, and P. C. Loewen. 1990. Regulation of transcription of katE and katF in Escherichia coli. J. Bacteriol. 172:6713-6720.[Abstract/Free Full Text]
31
31. Notley-McRobb, L., T. King, and T. Ferenci. 2002. rpoS mutations and loss of general stress resistance in Escherichia coli populations as a consequence of conflict between competing stress responses. J. Bacteriol. 184:806-811.[Abstract/Free Full Text]
32
32. Notley-McRobb, L., S. Seeto, and T. Ferenci. 2003. The influence of cellular physiology on the initiation of mutational pathways in Escherichia coli populations. Proc. R. Soc. London Ser. B Biol. Sci. 270:843-848.[Medline]
33
33. Ochman, H., J. G. Lawrence, and E. A. Groisman. 2000. Lateral gene transfer and the nature of bacterial innovation. Nature 405:299-304.[CrossRef][Medline]
34
34. Ochman, H., and R. K. Selander. 1984. Standard reference strains of Escherichia coli from natural populations. J. Bacteriol. 157:690-693.[Abstract/Free Full Text]
35
35. Peters, J. E., T. E. Thate, and N. L. Craig. 2003. Definition of the Escherichia coli MC4100 genome by use of a DNA array. J. Bacteriol. 185:2017-2021.[Abstract/Free Full Text]
36
36. Pupo, G. M., D. K. R. Karaolis, R. T. Lan, and P. R. Reeves. 1997. Evolutionary relationships among pathogenic and nonpathogenic Escherichia coli strains inferred from multilocus enzyme electrophoresis and mdh sequence studies. Infect. Immun. 65:2685-2692.[Abstract]
37
37. Rudd, K. E., B. R. Bochner, M. Cashel, and J. R. Roth. 1985. Mutations in the spoT gene of Salmonella typhimurium: effects on his operon expression. J. Bacteriol. 163:534-542.[Abstract/Free Full Text]
38
38. Stearns, S. M. 1992. The evolution of life histories. Oxford University Press, Oxford, United Kingdom.
39
39. Sutton, A., R. Buencamino, and A. Eisenstark. 2000. rpoS mutants in archival cultures of Salmonella enterica serovar Typhimurium. J. Bacteriol. 182:4375-4379.[Abstract/Free Full Text]
40
40. Tanaka, K., Y. Takayanagi, N. Fujita, A. Ishihama, and H. Takahashi. 1993. Heterogeneity of the principal sigma factor in Escherichia coli: the rpoS gene product, sigma 38, is a second principal sigma factor of RNA polymerase in stationary-phase Escherichia coli. Proc. Natl. Acad. Sci. USA 90:3511-3515. (Erratum, 90:8303.)[Free Full Text]
41
41. Velicer, G. J., and R. E. Lenski. 1999. Evolutionary trade-offs under conditions of resource abundance and scarcity: experiments with bacteria. Ecology 80:1168-1179.[CrossRef]
42
42. Visick, J. E., and S. Clarke. 1997. RpoS- and OxyR-independent induction of hpi catalase at stationary phase in Escherichia coli and identification of rpoS mutations in common laboratory strains. J. Bacteriol. 179:4158-4163.[Abstract/Free Full Text]
43
43. Waterman, S. R., and P. L. Small. 1996. Characterization of the acid resistance phenotype and rpoS alleles of Shiga-like toxin-producing Escherichia coli. Infect. Immun. 64:2808-2811.[Abstract]
44
44. Welch, R. A., V. Burland, G. Plunkett, P. Redford, P. Roesch, D. Rasko, E. L. Buckles, S. R. Liou, A. Boutin, J. Hackett, D. Stroud, G. F. Mayhew, D. J. Rose, S. Zhou, D. C. Schwartz, N. T. Perna, H. L. T. Mobley, M. S. Donnenberg, and F. R. Blattner. 2002. Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli. Proc. Natl. Acad. Sci. USA 99:17020-17024.[Abstract/Free Full Text]
45
45. Zambrano, M. M., D. A. Siegele, M. Almiron, A. Tormo, and R. Kolter. 1993. Microbial competition: Escherichia coli mutants that take over stationary phase cultures. Science 259:1757-1760.[Abstract/Free Full Text]

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Journal of Bacteriology, September 2004, p. 5614-5620, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5614-5620.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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