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Journal of Bacteriology, September 2004, p. 6335-6339, Vol. 186, No. 18
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.18.6335-6339.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Processing of the Tail Lysozyme (gp5) of Bacteriophage T4

Nanzhang Ye^1* and Naoki Nemoto²

Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Midoriku, Yokohama,¹ and Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Japan²

Received 9 October 2003/ Accepted 14 June 2004

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The processing site of gp5 has been determined to be between residues Val-390 and His-391, instead of Ser-351 and Ala-352 as previously reported (H. Kanamaru, N. C. Gassner, N. Ye, S. Takeda, and F. Arisaka, J. Bacteriol. 181:2739-2744). Moreover, the maturation of gp5 is abolished by null mutations in other hub genes, indicating that cleavage requires the interactions of several baseplate proteins.

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gp5 is an essential structural component in the hub of T4 baseplates (10). Expression of a cloned gene 5 resulted in the synthesis of a protein with a mass of about 63 kDa, consistent with the size predicted from the gene sequence (14) but about 20 kDa greater than the size of gp5 present in the hub. This led to the suggestion that a 20-kDa fragment was proteolytically removed from the gp5 precursor during assembly of the baseplate and that at least one phage protein was required for processing (14). A recent report (9) contradicting earlier results (14) suggested that the 75-kDa precursor was synthesized from a cloned gene 5 and that it was cleaved spontaneously or by a host enzyme at residue 351. To resolve this issue, N-terminal and C-terminal sequence analysis was performed on mature gp5 isolated from T4 tube baseplates, and polypeptides synthesized from several cloned truncated derivatives of gene 5 were compared with the mature gp5.

N-terminal sequence analysis of the gene products detached from tube baseplates and C-terminal sequence analysis of mature gp5. All the media and buffers used in this study were described previously (1, 2, 19). Phage tails and tube baseplates were prepared according to published procedures (15, 21). Purified tube baseplates were treated with 6 M urea at 37°C for 15 min or sodium dodecyl sulfate (SDS) sample buffer at 70 and 90°C for 3 min each and were subjected to SDS-polyacrylamide gel electrophoresis (PAGE) as described by Laemmli (13). Urea treatment overcame the problem of gp5 and gp48 comigrating and being difficult to resolve (15). As shown in Fig. 1, when the tube baseplates were treated with 6 M urea, gp5 as well as gp29, gp27, gp26, gp53, gp3, and gp25 was released from the tube baseplates but gp48 was not (Fig. 1, lane A). Because all hub proteins except gp28, the inside wedge proteins gp53 and gp25 (which are close to the hub), and the tail tip protein gp3 were released from baseplates, the results showed that the centers of the baseplates including the hubs were dissociated during urea treatment. Since gp28 was not detected in this experiment, the results indicated that gp28 is not a hub structural protein, as reported previously (10, 11). When the tube baseplates were treated with SDS buffer at 70°C, two bands appeared in the 24-kDa region (Fig. 1, lane B), the upper band being gp11 and the lower band being gp26 (confirmed by N-terminal sequence analysis) (Table 1). When the tube baseplates were treated at 90°C, the gp26 band comigrated with gp11 (Fig. 1, lane C). All the observed molecular weights of the gene products are close to the values calculated except for gp5 because of its processing. After electrophoresis, the proteins were transferred from the gel to a polyvinylidene difluoride (PVDF) membrane (20), and the bands of interest were subjected to automatic Edman degradation for N-terminal sequence analysis. The N-terminal sequences are almost identical to the DNA-deduced sequences except that the five N-terminal amino acid residues (MYEYK) are removed from gp26 (Table 1). Met-1 is retained or removed, consistent with reference 3. It was reported that the eight N-terminal residues of gp11 were cleaved (5) and gp26 was not located in the baseplate (10), but the present results showed that only Met-1 of gp11 is removed and gp26 is a component of the tail. One reason for the discrepancy may be interference from gp26, which comigrates with gp11 (Fig. 1, lane C).

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FIG. 1. SDS-PAGE analysis of gene products from tube baseplates. The polyacrylamide concentration was 12.5%. Tube baseplates were treated with 6 M urea at 37°C for 15 min (lane A), with SDS buffer at 70°C for 3 min (lane B), or with SDS buffer at 90°C for 3 min (lane C). Lane D, protein molecular weight standards.

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TABLE 1. N-terminal sequence analysis of some gene products detached from tube baseplates

For C-terminal sequence analysis of mature gp5, the gels were stained after electrophoresis (22) and gp5 was extracted from the excised bands (43 kDa) with a 50 mM (NH₄)HCO₃ solution containing 1% SDS at 4°C for 12 h three times. Approximately 680 pmol (quantitative analysis as described in reference 17) of gp5 was extracted from the gels and incubated with 1.2 U of carboxypeptidase (Sigma) at 25°C. The incubation was stopped (7) at 0, 15, 30, and 45 min. One cycle of Edman degradation was carried out for each sample. The results showed that carboxypeptidase digestion produced Val, Tyr, Pro, Tyr, and Glu in sequence, and all PTH amino acid peaks showed equivalent molar yields. There was only one sequence, Glu-Tyr-Pro-Tyr-Val, at positions 386 to 390, consistent with that deduced from the DNA sequence. None of the peaks corresponding to amino acids adjacent to the C-terminal Ser-351 reported in reference 9 were found. These findings indicated that the mature gp5 has a C-terminal Val-390, and the cleavage occurs between Val-390 and His-391, not between Ser-351 and Ala-352. The calculated molecular weight of 42,900 is consistent with the observed value, 42,000 to 43,000. The calculated isoelectric point of the mature gp5 was close to 8, similar to the reported value (15).

Expression and detection of gp5 polypeptides. Three truncated derivatives of gene 5 were cloned (16). After induction, they produced gp5 derivatives with C-terminal Ser-360, Val-380, or Val-390 (18). Because the pET-17b vector used in this study expresses an N-terminal fusion protein, the ribosomal binding site and other unnecessary sequences were removed from the vector by restriction with XbaI and EcoRI. The XbaI site and ribosome binding site sequences were included in the 5' primer upstream of the gene 5 start codon. The stop codon and the EcoRI site were included in three distinct 3' primers for PCR amplification of the three gene 5-truncated derivatives that expressed 360, 380, or 390 amino acid polypeptides. T4 dcDNA (Takara, Kyoto, Japan) was used as a template for PCR. The primers were synthesized by Bio/Can Scientific or Applied Biosystems Japan Inc. The three gp5-derived polypeptides were examined by Western blot analysis (6, 20) by using antiserum against gp5 (maintained and used in this laboratory). As shown in Fig. 2, the gp5 C-terminal Val-390 derivative comigrated with mature gp5 obtained from tube baseplates, consistent with the assignment of this cleavage site in gp5.

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FIG. 2. Recombinant gene 5-expressed protein products detected by Western blot analysis. After electrophoresis on SDS-12.5% PAGE, proteins were transferred to PVDF membranes. Lane A, Prestained protein molecular weight standards; lane B, gp5 separated from tube baseplates; lane C, sample from induced E. coli BL21(DE3) cells containing recombinant gene 5 with the codon for C-terminal Ser-360; lane D, sample from uninduced E. coli BL21(DE3) cells containing recombinant gene 5 with the codon for C-terminal Ser-360; lane E, sample from induced E. coli BL21(DE3) cells containing recombinant gene 5 with the codon for C-terminal Val-380; lane F, sample from uninduced E. coli BL21(DE3) cells containing recombinant gene 5 with the codon for C-terminal Val-380; lane G, sample from induced E. coli BL21(DE3) cells containing recombinant gene 5 with the codon for C-terminal Val-390; lane H, sample from uninduced E. coli BL21(DE3) cells containing recombinant gene 5 with the codon for C-terminal Val-390.

Examination of the processed gp5 from amber lysates. To identify the T4 gene products responsible for gp5 processing, T4 amber mutants with mutations in the baseplate genes were used to infect Escherichia coli BE (sup⁰) for preparation of amber lysates (1, 15), and mature gp5 was detected by Western blot analysis. We first interpreted the upper band in the blots (Fig. 3 and 4) as the uncleaved gp5 precursor or, perhaps, a cross-reacting E. coli protein. The results shown in Fig. 4 reveal that this band is gp23 because it has the correct size, 49 kDa (4), and it did not appear in the 23^– lane (Fig. 4, lane G). This indicated that gp23 has some amino acid sequences similar to gp5, because gp23 cross-reacted, as shown in Table 2 and Fig. 5. Since only the 26am N131 lysate gave rise to mature gp5, these results suggested that gp5 processing is a result of the interactions of several baseplate gene products and does not involve an E. coli enzyme or autolysis. If the processing occurred either via an E. coli enzyme or autolytically, the mature gp5 should be detected in all lysates (except for gene 5 amber lysate); however, this was not the case. On the other hand, because the gene 26 amber mutant is unable to form hubs (10) and the mature gp5 was detected in this lysate, gp5 processing may occur before hub formation. This suggests that gp5 enters the hub pathway after processing.

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FIG. 3. Western blot analysis of processed gp5 from lysates. After electrophoresis on SDS-8% PAGE, proteins were transferred to PVDF membranes. Lane A, 25am N67 lysate; lane B, 26am N131 lysate; lane C, 27am N120 lysate; lane D, 28am A452 lysate; lane E, 29am B7 lysate; lane F, tube baseplates; lane G, 5am B256 lysate; lane H, 48am NO22X lysate; lane I, 51am S29 lysate.

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FIG. 4. Western blot analysis of processed gp5 from lysates. After electrophoresis on SDS-8% PAGE, proteins were transferred to PVDF membranes. Lane A, prestained protein molecular mass standards (Bio-Rad Laboratories); lane B, 25am N67 lysate; lane C, 26am N131 lysate; lane D, 28am A452 lysate; lane E, 29am B7 lysate; lane F, 5am N135 lysate; lane G, 23am H11 lysate (tails); lane H, uninfected E. coli BE (sup0); lane I, 51am S29 lysate.

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TABLE 2. Amino acid fragments with similar sequences in gp5 and gp23

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FIG. 5. Similar regions of the amino acid sequence between gp5 (') and gp23 ("). The ratio of identical amino acid pairs in whole gp5/gp23 (1'/46" to 390'/435") is 8.2%, much higher than 5.0% in two common proteins. When the number of conservative changes is included, the total similarity ratio of amino acid pairs between gp5 and gp23 reaches 30.0%. In the main region from 155'/200" to 362'/407" (208 amino acid pairs, the region indicated by arrows), the ratio of identical amino acid pairs in gp5 and gp23 is 11.5% and the total similarity ratio including conservative changes is 35.1%. Colons indicate identical amino acids. Periods indicate conservative changes.

Western blot analysis of gp26. The assignment of gp26 as a band in SDS-PAGE had some ambiguity because gp26 comigrated with gp11 (Fig. 1). To identify gp26 on SDS-PAGE, an antiserum against a gp26 C-terminal peptide was prepared according to Iwai et al. (8), and Western blot analysis was carried out. As shown in Fig. 6, the 24-kDa band was found to be gp26, which was confirmed by N-terminal sequence analysis (Table 1). The molecular mass of gp26 was similar to that deduced from the nucleotide sequence, not 41 kDa as reported (12), and it is unlikely that the peptide cleaved from precursor gp5 was transferred to gp26 (14).

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FIG. 6. Western blot analysis of gp26. The tube baseplate proteins were transferred from SDS-12.5% PAGE gels to PVDF membranes. Lane A, gp26 band detected with antiserum against the C-terminal peptide of gp26; lane B, gp5 band detected with antiserum against gp5; lane C, gp26 and gp5 bands detected with a mixture of the two antisera.

In conclusion, these results demonstrate that gp5 is processed proteolytically, with a cleavage between Val-390 and His-391, resulting in the loss of 20 kDa and the conversion of the 63-kDa precursor gp5 to the 43-kDa mature gp5 found in the baseplate, and support the earlier sug-estion (14) that processing of gp5 depends on other T4 genes.

 
* Corresponding author. Present address: Nansho Kano, Department of Ecology, Malcom Co., Ltd., 15-10 Honmachi 4-chome, Shibuya-ku, Tokyo 151-0071, Japan. Phone: 81 3 3320 5611. Fax: 81 3 3320 5615. E-mail: nyeco{at}hotmail.com.

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Journal of Bacteriology, September 2004, p. 6335-6339, Vol. 186, No. 18
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.18.6335-6339.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ye, N. Articles by Nemoto, N. Search for Related Content PubMed PubMed Citation Articles by Ye, N. Articles by Nemoto, N.