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Journal of Bacteriology, December 2004, p. 7881-7887, Vol. 186, No. 23
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.23.7881-7887.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Purification and Initial Characterization of the Salmonella enterica PduO ATP:Cob(I)alamin Adenosyltransferase

Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida,¹ Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa²

Received 7 July 2004/ Accepted 27 August 2004

	ABSTRACT

Top Abstract Introduction Materials and Methods Results and Discussion References

 
The PduO enzyme of Salmonella enterica is an ATP:cob(I)alamin adenosyltransferase that catalyzes the final step in the conversion of vitamin B₁₂ to coenzyme B₁₂. The primary physiological role of this enzyme is to support coenzyme B₁₂-dependent 1,2-propanediol degradation, and bioinformatic analysis has indicated that it has two domains. Here the PduO adenosyltransferase was produced in Escherichia coli, solubilized from inclusion bodies, purified to apparent homogeneity, and partially characterized biochemically. The K_m values of PduO for ATP and cob(I)alamin were 19.8 and 4.5 µM, respectively, and the enzyme V_max was 243 nmol min^–1 mg of protein^–1. Further investigations showed that PduO was active with ATP and partially active with deoxy-ATP, but lacked measurable activity with other nucleotides. ³¹P nuclear magnetic resonance established that triphosphate was a product of the PduO reaction, and kinetic studies indicated a ternary complex mechanism. A series of truncated versions of the PduO protein were produced in Escherichia coli, partially purified, and used to show that adenosyltransferase activity is associated with the N-terminal domain. The N-terminal domain was purified to near homogeneity and shown to have biochemical properties and kinetic constants similar to those of the full-length enzyme. This indicated that the C-terminal domain was not directly involved in catalysis or substrate binding and may have another role.

	INTRODUCTION

Top Abstract Introduction Materials and Methods Results and Discussion References

 
Coenzyme B₁₂ (adenosyl-cobalamin [AdoCbl]) and closely related corrinoid compounds have a broad but uneven distribution in nature (1, 20, 24). These coenzymes are used by higher animals and many bacteria, but are apparently absent from plants and fungi. The primary physiological role of AdoCbl is in the catabolism of small molecules, including certain amino acids and carbohydrates Many prokaryotes synthesize this coenzyme de novo; however, higher animals and numerous other microbes depend on the assimilation of exogenous complex precursors such as vitamin B₁₂ (CNCbl).

The proposed pathways for CNCbl assimilation are thought to be similar in prokaryotes and eukaryotes (Fig. 1). The pathway initiates with the hydrolytic removal of the cyano-group by ß-ligand transferase to form HOCbl or glutathionylcobalamin. ß-Ligand transferase activity has been detected in rat liver, rabbit spleen, human leukocytes, human fibroblasts, and Propionibacterium shermanii and Clostridium tetanomorphum cell extracts (4, 18, 33); however, this enzyme or its encoding gene has not been isolated from any system (18). Assimilation proceeds with two one-electron reductions of the cobalt atom of HOCbl (which is in the 3⁺ oxidation state) to form cob(II)alamin and then cob(I)alamin. The reductases that catalyze these reactions have been identified in a variety of systems by biochemical assay, but genetic evidence supporting their roles in AdoCbl synthesis has not been reported (9, 13, 21, 28, 30-32). The final step in CNCbl assimilation is catalyzed by an ATP:cob(I)alamin adenosyltransferase which transfers a 5'-deoxyadenosyl moiety from ATP to the cobalt atom of cob(I)alamin, to form AdoCbl (5, 7, 10, 13, 25, 27). In addition to its role in the assimilation of CNCbl, this pathway is also needed for recycling of HOCbl formed from the breakdown of AdoCbl, which is unstable in vivo (12).

View larger version (23K): [in this window] [in a new window]   FIG. 1. Proposed pathway for the conversion of CNCbl to AdoCbl. This pathway is needed for the conversion of exogenous complex cobalamin precursors into AdoCbl as well as for the recycling of complex precursors generated intracellularly due to the breakdown of AdoCbl during catalysis.

Here, we report a facile procedure for the purification of the S. enterica PduO adenosyltransferase and partially characterize the purified enzyme biochemically. Investigations of truncated versions of the PduO enzyme confirm that adenosyltransferase activity is associated with its N-terminal domain. Kinetic studies indicate that the PduO enzyme is sufficient to mediate AdoCbl synthesis in vivo and that the enzyme uses a ternary complex mechanism. In addition, PduO is shown to have stringent nucleotide specificity and triphosphate is established as a reaction product.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results and Discussion References

 
Chemicals and reagents. Titanium (III) citrate was prepared as described previously (3). Hydroxycobalamin, sodium pyrophosphate, sodium triphosphate, and orthophosphate were from Sigma Chemical Company, St. Louis, Mo. Restriction enzymes and T4 DNA ligase were from New England Biolabs, Beverly, Mass. All other chemicals were from Fisher Scientific, Norcross, Ga.

Molecular techniques. Agarose gel electrophoresis was performed as described previously (16). Plasmid DNA was purified with QIAGEN (Chatsworth, Calif.) products according to the manufacturer's instructions. Following restriction enzyme digestion or PCR amplification, DNA was purified by phenol-chloroform extraction followed by ethanol precipitation or by use of QIAGEN gel extraction kits. Restriction digests were carried out according to standard protocols (16). For ligation of DNA fragments, T4 DNA ligase (New England Biolabs) was used according to the manufacturer's instructions. Bacteria were transformed by electroporation as described previously (14). Luria-Bertani (LB) medium containing antibiotic(s) was used to select for transformed cells, and single-colony isolates were used for analysis.

P22 transduction. Transductional crosses were performed as described previously with phage P22 HT105/1 int-201, a mutant phage that has high transducing ability (23). Transductants were selected with LB medium containing ampicillin (100 µg/ml). For the preparation of P22 transducing lysates from S. enterica TR6579, overnight cultures were grown with LB medium supplemented with 11 mM glucose and 11 mM galactose.

Protein techniques. Protein concentration was determined with Bio-Rad protein assay reagent according to the manufacturer's instructions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed with Bio-Rad Ready gels and Mini-protean II electrophoresis cells run at 200 V for 45 min with a Bio-Rad Power Pac 300. Following electrophoresis, proteins were stained with Coomassie brilliant blue R-250.

Cloning of truncated versions of the pduO gene for protein expression. DNA for cloning truncated versions of the pduO gene was obtained via PCR amplification of plasmid pMGS-2 (14) using forward primer 5'-GGAATTCAGATCTTATGGCGATTTATACCCGAAC-3' and reverse primer 5'-GCCGCCAAGCTTTCAAATCTCTTCGCTGCTGATGTA-3', 5'-GCCGCCAAGCTTTCACAGATAGCGCTTGCTAACTTC-3', 5'-GCCGCCAAGCTTTCAGTCAACGATGCTCACGACTAC-3', or 5'-GCCGCCAAGCTTTCAAAAGGTGACCACTTTTCCCTG-3'. The C-terminal domain of the pduO gene was amplified with primers 5'-GGAATTCAGATCTTATGCACAGCAAGGAGACGACGC-3' and 5'-GGAATTCAAGCTTGGTTTCGAGTTCAGAAGTATTC-3'. Pfu DNA polymerase (Stratagene, La Jolla, Calif.) was employed for the amplification. Amplified DNA was purified, restricted, and ligated to plasmid pTA925, a modified T7 expression vector (14) that had been previously digested with restriction enzymes BglII and HindIII, and gel purified. The ligation mixture was used to transform Escherichia coli DH5. LB medium containing kanamycin (25 µg/ml) was used to select transformants. Plasmid DNA was purified from selected transformants and screened by digestion with BglII and HindIII. Plasmids releasing fragments of the expected sizes (280, 565, 700, 889, and 462 bp) were used to transform the expression strain E. coli BL21(DE3) RIL (Stratagene). The DNA sequence of each clone was verified, and the resulting strains (BE294 to BE298) were used for production of truncated versions of the PduO enzyme. For complementation studies, truncated versions of the pduO gene were subcloned from the expression vectors into pLAC22 by using BglII and HindIII restriction sites. Vector pLAC22 allows the expression of cloned genes to be very stringently regulated (29). The resulting plasmids were used to transform S. enterica TR6579 by electroporation. Then, P22 transduction was used to transfer the subclones into S. enterica BE121 (cobA pduO) (14), and the resulting strains (BE299 to BE303) were used for complementation studies.

Production of recombinant PduO and truncated versions. Protein expression strains were grown in 1-liter baffled Erlenmeyer flasks containing 400 ml of LB broth and 25-µg/ml kanamycin. A 2-ml LB overnight culture was used as the inoculum, and cultures were incubated at 30°C in a gyratory water bath (New Brunswick) with shaking at 250 rpm, until an optical density at 600 nm of 0.5 was attained. The expression of recombinant protein was induced by the addition of isopropyl-ß-D-galactopyranoside (IPTG) to a final concentration of 1 mM, and cultures were incubated at 30°C for an additional 3 h after IPTG addition. Both the full-length protein and its truncated version produced inclusion bodies when expressed at high levels.

Purification of recombinant PduO and PduO^1-185 enzymes. Cells from 400-ml cultures were collected by centrifugation for 15 min at 10,500 x g with a Beckman (Fullerton, Calif.) J2-HS centrifuge and a Beckman JA-10 rotor. The pellet was suspended in 3 ml of buffer A (50 mM potassium phosphate at pH 7.5, 20 mM sodium chloride, 1 mM Pefabloc protease inhibitor [Pentapharm, Ltd., Basel, Switzerland]) and lysed by single pass through a French press at 137.9 Mpa. Insoluble material was collected by centrifugation at 27,500 x g for 30 min with a Beckman JA-20 rotor. The supernatant was discarded, and the insoluble pellet was treated with Bacterial Protein Extraction Reagent II (B-PER II; Pierce, Rockford, Ill.) according to the manufacturer's "midi-prep" protocol with the following modifications. The pellet was initially suspended in 5 ml of B-PER II, and following lysozyme treatment, 15 ml of 1:20 dilute B-PER II solution was added. The inclusion bodies were washed twice with 20 ml of 1:20 dilute B-PER II solution supplemented with 5 mM dithiothreitol and washed once with buffer A. The inclusion bodies were harvested by centrifugation and suspended in solubilization buffer [25 mM 2-(cyclohexylamino) ethanesulphonic acid (CHES) at pH 8.5, 1 mM dithiothreitol] to a final concentration of 5 mg/ml; solubilization was carried out overnight at 0°C. Insoluble material was separated from soluble protein by centrifugation at 27,500 x g for 10 min. The supernatant was recovered and filtered with a 0.22-µm-pore cellulose-acetate filter. The filtrate was applied to a 25-ml bed-volume phenyl Sepharose HP column (Amersham Biosciences, Uppsala, Sweden) that was equilibrated with 25 mM CHES at pH 8.5. PduO or PduO^1-185 were eluted with a linear gradient from 25 mM to 750 mM CHES at pH 8.5. Fractions containing the enzyme of interest were combined and concentrated with a Vivascience 10,000-molecular-weight-cutoff concentrator.

Partial purification of recombinant PduO and its truncated versions. For determination of the domain(s) required for adenosyltransferase activity, PduO and its truncated versions were partially purified by extraction of inclusion bodies using B-PER II followed by solubilization with CHES as described above for purification of recombinant PduO.

Adenosyltransferase assays. Adenosyltransferase assays were performed at 37°C and pH 8.0 with 50 µg of recombinant protein using the continuous spectrophotometric method described previously (14). Briefly, reaction mixtures were prepared in an anaerobic hood and contained 200 mM Tris-HCl at pH 8.0, 5 mM KCl, 2.5 mM MgCl₂, 0.5 mM ATP, 0.05 mM HOCbl, and 1 mM titanium (III) citrate in a final volume of 2 ml. The rate of AdoCbl production was calculated by using the average of at least three determinations.

Kinetic analyses. The adenosyltransferase reaction has two substrates. Therefore, in kinetic analyses, one substrate was used in excess while the concentration of the other was varied. To determine the dependence of adenosyltransferase activity on ATP concentration, hydroxycobalamin was used at 0.05 mM (11 times the K_m), and the ATP concentration was varied from 0 to 0.2 mM. When the effect of cob(I)alamin concentration on reaction rate was determined, ATP was used at 0.4 mM (20 times the K_m) and cob(I)alamin was varied from 0 to 0.05 mM. Assays contained 50 µg of purified PduO or PduO^1-185, and initial reaction rates were measured. (Typically <0.5% of the substrate was consumed during the assay period.) Each experiment was repeated three times.

³¹P NMR spectroscopy. The products of the PduO reaction were analyzed by ³¹P nuclear magnetic resonance (NMR) spectroscopy. The adenosyltransferase assay was performed in an anaerobic test tube containing 200 mM Tris-HCl at pH 8.0, 5 mM KCl, 2.5 mM MgCl₂, 2.0 mM ATP, 2.0 mM HOCbl, 5 mM titanium (III) citrate, and 500 µg of PduO protein in a final volume of 5 ml. The reaction mixtures were incubated in the dark at 37°C for 2 h. Samples from the reaction mixture were transferred to 12-mm NMR tubes. EDTA was added to a final concentration of 75 mM, and 0.5 ml of 100% D₂O was added. ³¹P NMR spectra of reference compounds and adenosyltransferase reaction products were obtained with a 7.0-T magnet (IFAS NMR Facility, University of Florida, Gainesville) and a modified Nicolet NT300 spectrometer (6) at the following settings: frequency, 121.469 MHz; excitation pulse width, 15 µs; pulse repetition delay, 6 s; and spectral width, 10 kHz. Spectra were Fourier transformed with Felix analytical software (Molecular Simulations, Inc., San Diego, Calif.). Chemical shifts were referenced to an inorganic phosphoric acid internal standard, which was set to 0.0 ppm.

	RESULTS AND DISCUSSION

Top Abstract Introduction Materials and Methods Results and Discussion References

 
Purification of PduO adenosyltransferase. The S. enterica PduO protein was produced at high levels in E. coli, using a modified T7 expression vector as previously described (14). When produced in this manner, the PduO protein is found in the insoluble fraction of cell extracts but is catalytically active (14). The PduO adenosyltransferase was solubilized in an active form by using CHES buffer, which has detergent-like properties, and purified to near homogeneity by hydrophobic interaction chromatography. Following the phenyl Sepharose chromatography, only fractions of the highest purity were saved for further study. The progress of the purification was monitored by SDS-PAGE and enzymatic assay (Table 1). Following staining with Coomassie brilliant blue, the purified PduO protein appeared homogenous (not shown). The yield was 23%, and the fold purification was 1.4. The relatively low fold purification is a consequence of the fact that the inclusion bodies were composed primarily of the PduO enzyme. The finding that the yield was >100% following solubilization with CHES suggests that the activity of PduO is inhibited somewhat by incorporation into inclusion bodies. Overall, the purification allowed the isolation of 20 mg of purified PduO in a 2-day period.

Linearity and requirements of the PduO reaction. The effect of enzyme concentration on the rate of adenosyltransfer was examined. Adenosyltransferase activity was directly proportional to the PduO concentration in the range of 10 to 200 µg of protein (rate, 197 nmol min^–1 mg^–1; and R² = 0.9928). The omission of PduO, ATP, HOCbl, or titanium (III) citrate—the reducing agent that converts HOCbl to cob(I)alamin—eliminated measurable activity.

Divalent metal ion requirements of the PduO reaction. Adenosyltransferase assays were performed under standard conditions, except that Mg²⁺ was omitted or replaced with other divalent metal ions. Without added divalent metal, adenosyltransferase activity of 91 nmol min^–1 mg^–1 (32%) was observed. The highest activities were obtained with Mg²⁺, Mn²⁺, or Co²⁺ (285, 277, or 266 nmol of AdoCbl min^–1 mg^–1, respectively). Added Ca²⁺ had little effect on activity (81 nmol min^–1 mg^–1), and Cd²⁺, Zn²⁺, Ni²⁺, or Cu²⁺ abolished measurable activity.

The divalent metal ion requirements of PduO are similar to those reported for the T. acidophilum adenosyltransferase, which was also most active with Mg²⁺ (22). However, they are somewhat different from results obtained with the CobA and C. tetanomorphum enzymes, both of which showed maximal activity when Mn²⁺ was included in the reaction mixture and partial activity with Mg²⁺ or Co²⁺ (25, 27).

The N-terminal domain of PduO has adenosyltransferase activity. Bioinformatic analyses indicated that the S. enterica PduO protein has two domains (14). The N-terminal domain corresponds to amino acids 1 to 185, and the C-terminal domain is composed of amino acids 186 to 336. To investigate the functions of these domains, a series of truncated versions of the PduO protein were produced in E. coli, partially purified, and assayed for adenosyltransferase activity. The polypeptides produced were composed of the first 90, 185, 230, and 293 amino acids of the PduO protein (PduO^1-90, PduO^1-185, PduO^1-230, and PduO^1-293). Each protein was partially purified from inclusion bodies by treatment with B-PER II followed by solubilization with CHES buffer, and SDS-PAGE indicated that each protein was 80 to 90% pure (Fig. 2). Enzyme assays demonstrated that PduO^1-185, PduO^1-230, and PduO^1-193 (each of which has deletions of all or part of the C-terminal domain) had adenosyltransferase activity, with turnover numbers similar to that of the full-length PduO protein (Table 2). The specific activities of these truncated proteins are higher than that of the full-length protein because of their reduced molecular masses. In contrast, disruption of the N-terminal domain (PduO^1-90) abolished measurable adenosyltransferase activity. Thus, in vitro studies indicated that adenosyltransferase activity of PduO is associated with its N-terminal domain.

View larger version (76K): [in this window] [in a new window]   FIG. 2. SDS-PAGE analysis of solubilized truncated versions of the PduO protein. Each lane contains 4 µg of recombinant protein. Lane 1, molecular mass markers; lane 2, PduO; lane 3, PduO1-293; lane 4, PduO1-230; lane 5, PduO1-185, lane 6, PduO1-90. PduO and its truncated versions were partially purified as described in Materials and Methods.

Kinetic measurements. In vitro assays indicated that both PduO and PduO^1-185 followed Michaelis-Menten kinetics with respect to both ATP and cob(I)alamin. Using the Eadie-Hofstee method, the K_m values of PduO and PduO^1-185 were determined to be 19.8 and 19.3 µM for ATP and 4.5 and 4.3 µM for cob(I)alamin, respectively. The V_maxs were 243 nmol min^–1 mg^–1 for PduO and 452 nmol min^–1 mg^–1 for PduO^1-185. The turnover numbers (k_cat) for PduO and PduO^1-185 were similar (9.64 and 9.04 min^–1), indicating that the higher V_max of PduO^1-185 was the result of its lower molecular mass but otherwise was not related to deletion of the C-terminal domain.

The kinetic constants for PduO are comparable to those previously reported for bacterial adenosyltransferase enzymes, for which specific activities range from 53 to 619 nmol min^–1 mg^–1 and K_m values ranged from 2.8 to 110 µM for ATP and from 3 to 5.2 µM for cob(I)alamin (22, 25, 27). These values are appropriate to the physiological role of adenosyltransferase enzymes, since cellular levels of cobalamin are typically 1 to 10 µM in prokaryotic organisms and ATP concentrations are in the low millimolar range (1, 24). Thus, the kinetic properties of PduO indicate that this enzyme is sufficient to meet cellular needs for AdoCbl synthesis.

Specificity for the donor nucleotide. The abilities of PduO and PduO^1-185 to use nucleotides other than ATP as the donor molecule for the ß ligand of cobalamin were measured. PduO and PduO^1-185 showed no measurable catalytic activity when adenine, adenosine, AMP, ADP, CTP, GTP, UTP, or 2'-deoxyadenosine was used in place of ATP, even after prolonged incubation (up to 30 min) at 37°C. The PduO adenosyltransferase displayed partial activity (21%) when 2'-deoxyadenosine-5'-triphosphate (dATP) was used in place of ATP (44 compared to 210 nmol min^–1 mg^–1).

The findings that PduO has stringent nucleotide specificity and partial activity (21%) with dATP are similar to results recently reported for the adenosyltransferase from the archaeon T. acidophilum (22). The archaeal enzyme is 177 amino acids in length and 27% identical to the N-terminal domain of the PduO enzyme. It differs from the PduO enzyme in that it lacks a C-terminal domain and is not known to be associated with a polyhedral body (22). Nonetheless, these results are suggestive that the PduO and T. acidophilum enzymes may bind ATP in a similar manner.

In contrast to the PduO and T. acidophilum adenosyltransferases, the CobA enzyme has a relatively low nucleotide specificity and is inactive with dATP under standard assay conditions (8). Previously the three-dimensional structure of the CobA adenosyltransferase complexed with MgATP was determined (2). The CobA protein contains a modified Walker A motif, defined by residues Gly³⁶ to Thr⁴³ (GNGKGKTT), which associates with the -, ß-, and -phosphates of ATP via extensive hydrogen bonding (2). Fewer hydrogen bonds are found between the adenosine moiety and the CobA protein, which may explain its relatively low nucleotide specificity (2, 8). In addition, the -phosphate and 2'-OH of ATP form an intramolecular hydrogen bond which is proposed to be critical for catalysis, accounting for the lack of activity of CobA with dATP (8). In contrast, the sequences of the PduO and T. acidophilum adenosyltransferases have no distinguishable P-loop or Walker motif, and both have partial activity with dATP, indicating an alternative mode of ATP binding as well as possible differences in the catalytic mechanisms (22).

Triphosphate is the product of the PduO adenosyltransferase reaction. Prior studies demonstrated that AdoCbl was a product of the PduO adenosyltransferase reaction, but the fate of the triphosphate moiety of ATP was not determined (14). To address this question, ³¹P NMR spectra were determined for the substrates and products of the PduO reaction (Fig. 3). Fig. 3A shows the ³¹P NMR spectrum of an adenosyltransferase reaction mixture prior to addition of the PduO enzyme. The triplet centered at –21.8 ppm corresponds to the ß-phosphate of ATP; the two doublets centered at –6.2 ppm and –11.2 ppm correspond to the - and -phosphates of ATP, respectively. The singlet at –0.7 ppm results from the phosphate group that is in the nucleotide loop of the cobalamin. Fig. 3B shows the complete reaction mixture after 2 h of incubation at 37°C with 500 µg of purified PduO. The doublet corresponding to the -phosphate of ATP (–11.2 ppm) is absent, indicating that the reaction went to completion. The triplet centered at –20.7 ppm and the doublet centered at –6 ppm of the complete reaction mixture correlate well with the triplet at –20.3 ppm and the doublet at –5.8 ppm of the sodium triphosphate standard (Fig. 3C). The singlet at –1.4 ppm corresponds to the phosphate group of the nucleotide loop of AdoCbl, since for the authentic AdoCbl standard, this phosphate had a chemical shift of –1.4 ppm. The shift of this resonance upfield from –0.7 ppm to –1.4 ppm was likely caused by the change in conformation of the corrin ring that occurs when the 5'-deoxyadenosyl group is added to the ß-axial position. It is also of note that the spectrum of the completed reaction (Fig. 3B) lacked a singlet at –6.7 ppm which is characteristic of pyrophosphate, demonstrating that this compound was not a major reaction product. Hence, these results indicate that triphosphate and AdoCbl are the products of the PduO reaction and that significant hydrolysis of the triphosphate to orthophosphate and pyrophosphate did not occur.

View larger version (15K): [in this window] [in a new window]   FIG. 3. 31P NMR spectra of a PduO reaction. All chemical shifts are referenced to H3PO4, which is set to 0 ppm. (A) Reaction mixture prior to addition of PduO containing 2 mM ATP and 2 mM HOCbl; chemical shifts observed at –6.2 ppm, –11.2 ppm, and –21.8 ppm correspond to the -, -, and ß-phosphates of ATP, respectively; the singlet at –0.7 ppm corresponds to the phosphate of the nucleotide loop of HOCbl. (B) Complete reaction after 2 h of incubation with 500 µg of purified PduO. (C) Inorganic triphosphate standard (0.1 M; center phosphate, triplet centered at –20.3 ppm; outer phosphates, doublet centered at –5.8 ppm).

Inhibition of the PduO reaction by nucleotides other than ATP. To test for possible inhibitory effects of nucleotides other than ATP on the PduO reaction, adenosyltransferase assays were conducted that contained 50 µM ATP and 100 µM competing nucleotide. PduO retained 95% activity when AMP, CTP, GTP, or UTP was added to reaction mixtures (Fig. 4). When adenine, adenosine, or 2'-deoxyadenosine was included, 90% activity was retained. However, only 10% activity remained when ADP was included, and kinetic analyses in which the concentrations of both ATP and ADP were varied indicated a K_i of 4.9 µM (data not shown). The finding that ADP is inhibitory whereas AMP is not suggests a role for the ß-phosphate in the binding of ATP to the PduO adenosyltransferase.

View larger version (64K): [in this window] [in a new window]   FIG. 4. Inhibition of PduO by nucleotides other than ATP and adenine-containing compounds. Adenosyltransferase assays were performed with 25 µg of purified PduO protein. The concentration of ATP used was 50 µM, and the concentration of the inhibitor was 100 µM. The chart shows the percentage of adenosyltransferase remaining in the presence of inhibitor. 2' d'Ado, 2'-deoxyadenosine.

Evidence for a ternary complex. In order to determine whether a covalent intermediate existed in the reaction mechanism, the effects of various concentrations of both ATP and HOCbl on the rate of adenosyltransfer were examined. For these studies, HOCbl was used at a concentration of 2, 4, 6, 8, 10, or 20 µM, as the ATP concentration was varied from 10 to 100 µM, and the data were analyzed by the double-reciprocal method of Lineweaver and Burk (Fig. 5). Results showed that both the slope and the x intercept of the Lineweaver-Burk plot vary with the concentration of either ATP or HOCbl. This suggests that PduO employs a ternary complex reaction mechanism rather than a substituted enzyme mechanism. Many ATP-dependent enzymes employ random ternary complex mechanisms during catalysis, and this may be the case for the PduO adenosyltransferase, although these studies do not rule out an ordered ternary complex mechanism.

View larger version (34K): [in this window] [in a new window]   FIG. 5. Double-reciprocal plots showing the effect of varying both ATP and HOCbl concentrations on the rate of adenosyltransferase activity. Adenosyltransferase assays were performed with 25 µg of purified PduO protein. HOCbl (HOB12) was held constant at 2, 4, 6, 8, 10, or 20 µM, while the concentration of ATP was varied from 10 to 100 µM. The experiment was repeated three times with similar results, and the average of the three experiments is shown.

	ACKNOWLEDGMENTS

 
This work was supported by grant DK064771 from the National Institutes of Health.

We thank J. F. Preston, D. L Purich, and K. T. Shanmugam for invaluable assistance.

	FOOTNOTES

 
* Corresponding author. Mailing address: Iowa State University, Department of Biochemistry, Biophysics and Molecular Biology, 2164 MBB, Ames, IA 50011. Phone: (515) 294-4165. Fax: (515) 294-0453. E-mail: bobik{at}iastate.edu.

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