This Article Abstract Full Text (PDF) Supplemental material Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kocan, M. Articles by Bott, M. Search for Related Content PubMed PubMed Citation Articles by Kocan, M. Articles by Bott, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 2006, p. 724-732, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.724-732.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Two-Component Systems of Corynebacterium glutamicum: Deletion Analysis and Involvement of the PhoS-PhoR System in the Phosphate Starvation Response

Martina Koan, Steffen Schaffer,^, Takeru Ishige, Ulrike Sorger-Herrmann, Volker F. Wendisch, and Michael Bott^*

Institut für Biotechnologie 1, Forschungszentrum Jülich, D-52425 Jülich, Germany

Received 15 July 2005/ Accepted 22 October 2005

	ABSTRACT

Top Abstract Introduction Materials and Methods Results Discussion References

 
Corynebacterium glutamicum contains genes for 13 two-component signal transduction systems. In order to test for their essentiality and involvement in the adaptive response to phosphate (P_i) starvation, a set of 12 deletion mutants was constructed. One of the mutants was specifically impaired in its ability to grow under P_i limitation, and therefore the genes lacking in this strain were named phoS (encoding the sensor kinase) and phoR (encoding the response regulator). DNA microarray analyses with the C. glutamicum wild type and the phoRS mutant supported a role for the PhoRS system in the adaptation to P_i starvation. In contrast to the wild type, the phoRS mutant did not induce the known P_i starvation-inducible (psi) genes within 1 hour after a shift from P_i excess to P_i limitation, except for the pstSCAB operon, which was still partially induced. This indicates an activator function for PhoR and the existence of at least one additional regulator of the pst operon. Primer extension analysis of selected psi genes (pstS, ugpA, phoR, ushA, and nucH) confirmed the microarray data and provided evidence for positive autoregulation of the phoRS genes.

	INTRODUCTION

Top Abstract Introduction Materials and Methods Results Discussion References

 
Phosphorus (P) is an essential nutrient for all cells and required, e.g., for the biosynthesis of nucleotides, DNA, and RNA and in addition for the functional regulation of protein activity by phosphorylation. The common phosphorus source is inorganic phosphate (P_i), and cells have developed mechanisms for the acquisition, assimilation, and storage of phosphate. Under phosphate starvation, many bacteria induce the synthesis of proteins that enable them to use the limiting phosphate resources more efficiently and to make alternative phosphorus sources accessible. The corresponding genes are collectively named P_i starvation-inducible genes, or psi genes. The phosphate starvation response, in particular its regulation, has been most carefully studied in Escherichia coli (33) and Bacillus subtilis (11). In both species, two-component signal transduction systems consisting of a histidine kinase and a response regulator play a prominent role.

In E. coli, induction of the P_i starvation genes is dependent on the PhoR-PhoB two-component system. Under P_i limitation, the histidine kinase PhoR phosphorylates the response regulator PhoB, and PhoBP in turn activates transcription of at least 31 genes, which form the Pho regulon (33). The genes include the phoBR operon; the pstSCAB-phoU operon, encoding an ABC transporter for high-affinity P_i uptake and a regulatory protein; the ugpBAECQ operon, encoding an sn-glycerol 3-phosphate ABC uptake system and glycerophosphoryl diester phosphodiesterase; the phoA-psiF operon, encoding alkaline phosphatase and a protein of unknown function; phoE, encoding an anion-specific porin; phoH, encoding an ATP-binding protein of unknown function; and the phnCDEFGHIJKLMNOP operon, encoding proteins involved in the uptake of phosphonates and their degradation via the C-P lyase pathway. Thus, when P_i is scarce, E. coli takes up P_i by an ATP-driven high-affinity transport system, mobilizes P_i outside the cytoplasm by phosphatases and esterases, and forms proteins for the uptake and degradation of organophosphates and phosphonates.

In B. subtilis, the response to P_i starvation is more complex, since it involves the induction both of a specific Pho regulon and of the ^B-dependent general stress response. The Pho regulon is controlled by a two-component system composed of the histidine kinase PhoR and the response regulator PhoP. It includes the phoPR operon; phoA and phoB for two alkaline phosphatases; phoD for an alkaline phosphatase/phosphodiesterase with a putative role in cell wall teichoic-acid turnover; the pstSACB1B2 operon; the tuaABCDEFGH operon, encoding proteins which are responsible for the synthesis of teichuronic acid, which replaces the teichoic acid in the cell walls of phosphate-starved cells; glpQ, encoding glycerophosphoryl diester phosphodiesterase; and ydhF, encoding a lipoprotein (2, 11). The tagAB and tagDEF operons, which encode protein involved in teichoic acid biosynthesis, are repressed by PhoP under P_i starvation (20). Besides PhoR-PhoP, the ResD-ResE two-component system, the response regulator Spo0A, and the AbrB regulator are also involved in the control of Pho regulon genes (11).

Corynebacterium glutamicum is a gram-positive bacterium belonging to the order Actinomycetales and has gained considerable interest because of its use in the large-scale biotechnological production of L-glutamate and L-lysine (6). Phosphorus constitutes 1.5% to 2.1% of the cell dry weight of C. glutamicum (18), part of which is present as polyphosphate (16, 24). In a previous study, we analyzed the P_i starvation response of C. glutamicum by using whole-genome DNA microarrays (12). The comparison of the mRNA profiles before and at different times after a shift from P_i excess to P_i starvation led to the identification of a group of genes that are specifically required to cope with a limited P_i supply. This group includes the pstSCAB operon, encoding an ABC transporter for high-affinity P_i uptake; the ugpAEBC operon, encoding an ABC transporter for the uptake of glycerol 3-phosphate; glpQ1, encoding a glycerophosphoryl diester phosphodiesterase; ushA, encoding a secreted enzyme with UDP sugar hydrolase and 5'-nucleotidase activity (27); nucH, encoding a putative secreted nuclease which possibly serves a role in liberating P_i from extracellular nucleic acids; NCgl2959/Cg3393, which may encode a cell wall-associated phosphatase (34); phoH1, encoding an ATPase of unknown function; and the pctABCD operon, encoding an ABC transport system which might be involved in the uptake of a yet-unknown phosphorus-containing compound (12).

In parallel to the elucidation of the P_i starvation stimulon of C. glutamicum, we initiated studies aimed at the identification of the regulators controlling the P_i starvation response. Evidence derived from other bacteria showed that two-component regulatory systems are prime candidates for this function. Here we present evidence that one of the 13 two-component systems of C. glutamicum, named PhoS-PhoR, is involved in the adaptation of C. glutamicum to limiting P_i concentrations.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results Discussion References

 
Bacterial strains and growth conditions. Bacterial strains, plasmids, and oligonucleotides used or constructed for this work are listed in Tables S1, S2, and S3, respectively (see the supplemental material). For the construction of plasmids, E. coli DH5 was used as a host and routinely grown aerobically at 37°C on a rotary shaker (120 rpm) in LB medium (10 g/liter tryptone, 5 g/liter yeast extract, 5 g/liter NaCl) or on LB agar plates (LB medium with 1.5% [wt/vol] agar). C. glutamicum ATCC 13032 (1) and its derivatives were cultivated aerobically on a rotary shaker (120 rpm) at 30°C either in LB medium, in brain heart infusion medium supplemented with 0.5 M sucrose, in CGIII medium (21), or in CGXII minimal medium (15) containing 30 mg/liter protocatechuic acid as an iron chelator and 40 g/liter glucose as a carbon and energy source. CGXII medium contains 13 mM phosphate in the forms of KH₂PO₄ and K₂HPO₄ as its sole phosphorus source. In order to obtain phosphate-limited conditions (0.065 mM), the concentration of these two compounds was reduced from 1 g/liter to 0.005 g/liter. If appropriate, kanamycin was added to a final concentration of 25 µg/ml (C. glutamicum) or 50 µg/ml (E. coli).

For an analysis of the influence of different phosphate concentrations on the growth of C. glutamicum strains, they were first grown overnight in CGIII medium. After being washed, the cells were cultured for 24 h in CGXII medium under P_i-limiting conditions (0.065 mM) and then inoculated into CGXII medium containing either 0.065 mM phosphate (P_i limitation) or 13 mM phosphate (P_i excess). Growth was followed by a measurement of the optical density at 600 nm (OD₆₀₀).

For an analysis of the response of C. glutamicum to a shift from P_i excess to P_i limitation by DNA microarray or primer extension analysis, cells of the wild type or the phoRS mutant were first precultured in CGIII medium and then grown for 24 h in CGXII medium under P_i-sufficient conditions and finally inoculated into the same medium to an initial OD₆₀₀ of 0.6. Exponentially growing cells from this P_i-sufficient culture (OD₆₀₀, 4 to 5) were harvested and washed. RNA was prepared from one aliquot, whereas the other aliquot was used to inoculate cultures with a medium containing a limiting P_i concentration (0.065 mM). RNA was prepared 10, 30, 60, or 90 min after the P_i downshift.

Construction of C. glutamicum deletion mutants. Deletion mutants of C. glutamicum lacking citAB, cgtSR1, cgtRS2, cgtRS3, cgtRS5, cgtRS6, cgtSR7, cgtSR8, cgtRS9, cgtSR10, or cgtSR11 were constructed via a two-step homologous-recombination procedure involving crossover PCR (19) and the suicide vector pK19mobsacB (28) as described previously (23). The deletions were verified by PCR using primers annealing outside the regions involved in recombination (e.g., oligonucleotides cgtRS3-out-fw and cgtRS3-out-rv). In the cases of the phoRS (=cgtRS3), citAB, and cgtRS9 mutants, Southern blot analysis was also performed (23) and confirmed the deletions (data not shown).

Construction of plasmid pEKEx2-phoRS. The phoRS coding sequence was amplified from the chromosomal DNA of the C. glutamicum wild type by PCR using the oligonucleotides phoRS-SbfI-fw and phoRS-KpnI-rv (Table S3 in the supplemental material) and the Expand High Fidelity PCR system (Roche Diagnostics, Mannheim, Germany). The primer phoRS-SbfI-fw introduces an SbfI restriction site 40 bp upstream of the phoR start codon. The primer phoRS-KpnI-rv introduces a KpnI restriction site after the stop codon of phoS and adds eight codons (WSHPQFEK) before the stop codon, leading to a PhoS protein with a carboxy-terminal StrepTag-II (29). The resulting PCR product (2,263 bp) was cleaved with SbfI and KpnI, gel purified using the QIAEX kit (QIAGEN, Hilden, Germany), and cloned into the E. coli-C. glutamicum shuttle vector pEKEx2 (7) cut with the same enzymes, resulting in plasmid pEKEx2-phoRS. DNA sequence analysis confirmed that the cloned phoRS genes were identical to the published wild-type sequence (13).

Preparation of total RNA for DNA microarray and primer extension experiments. RNA from C. glutamicum was prepared from 20-ml culture aliquots using the RNeasy kit (QIAGEN, Hilden, Germany) with on-column DNase I treatment as described previously (22). Isolated RNA samples were checked for purity by denaturing formaldehyde agarose gel electrophoresis, quantified by UV spectrophotometry, and stored at –70°C until use.

DNA microarray analysis. Whole-genome DNA microarrays for C. glutamicum were generated as described previously (12, 17). Identical amounts (20 to 25 µg) of total RNA were used for random hexamer-primed synthesis of fluorescently labeled cDNA by reverse transcription with Superscript II (Invitrogen, Karlsruhe, Germany) and the fluorescent nucleotide analogues FluoroLink Cy3-dUTP (green) and Cy5-dUTP (red) (Amersham Pharmacia, Little Chalfont, United Kingdom) as described previously (17). The labeled cDNA probes were purified and concentrated by using Microcon YM-30 filter units (Millipore, Bedford, Mass.). Subsequently, the mixed Cy3- and Cy5-labeled cDNAs containing 1.2 µg poly(A)/ml (Sigma, Munich, Germany) as a competitor, 30 mM HEPES, and 0.3% sodium dodecyl sulfate in 3x SSC (1x SSC is 0.15 M NaCl plus 0.015 M sodium citrate) were hybridized to whole-genome arrays in a humid chamber for 5 to 16 h at 65°C. After hybridization, the arrays were washed in a mixture of 1x SSC and 0.03% sodium dodecyl sulfate and finally in 0.05x SSC. Immediately after stringent washing, the fluorescence intensities at 635 and 532 nm were determined using a GenePix 4000 laser scanner (Axon, Inc., Union City, Calif.), and the images were processed by using GenePix 3.0 software. Data were normalized to the average ratio for C. glutamicum genomic DNA. The normalized ratio of median fluorescence was taken to reflect the relative RNA abundance for spots with green or red fluorescent signals that were at least threefold greater than the median fluorescence background signal. For statistical analysis of the gene expression data, P values for the independent replicate experiments were calculated based on the Student t test by using log-transformed fluorescence ratios for individual genes on the one hand and for genomic DNA on the other hand. In a first series of experiments (performed in triplicate), the mRNA levels of the phoRS mutant 10, 30, and 60 min after a shift from P_i excess (13 mM) to P_i limitation (0.065 mM) were compared to the mRNA levels before the shift (zero time). In a second series of experiments, the mRNA levels of the wild type were compared with the mRNA levels of the phoRS mutant before and 60 min after a shift from P_i excess to P_i limitation.

Primer extension analysis. Nonradioactive primer extension analysis of the genes pstS, ugpA, phoR, ushA, and nucH was performed as described previously (8, 9) using IRD800-labeled oligonucleotides (Table S3 in the supplemental material) and 20 µg isolated RNA as a template. The lengths of the primer extension products were determined by running the four lanes of a DNA-sequencing reaction mixture set up using the same oligonucleotide as that usedfor reverse transcription alongside the primer extension products. The templates for DNA sequencing were obtained by PCR using the oligonucleotide pairspstS_promreg_fw/pstS_promreg_rv, ugpA_promreg_ fw/ugpA_promreg_rv,phoR_promreg_fw/phoR_promreg_rv, ushA1/ushA2, and nucH1/nucH2 (for sequences, see Table S3 in the supplemental material).

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

 
In silico and deletion analyses of C. glutamicum two-component systems. During the annotation of the C. glutamicum genome sequence (13), genes encoding 13 sensor kinases and 13 response regulators were identified. In Fig. 1, maps of the corresponding genomic loci and the designations given to the corresponding genes are shown. As is evident from this figure, each sensor kinase gene is paired with a response regulator gene, suggesting that the corresponding proteins form the cognate partners of a typical two-component signal transduction system. No orphan sensor kinase or response regulator genes are present in the genome. Bioinformatic analysis indicated that each of the 13 sensor kinases possesses at least one transmembrane helix, indicating that all are integral membrane proteins (Table S4 in the supplemental material). All sensor kinases contained the two domains that are characteristic for these proteins, i.e., the histidine kinase acceptor domain containing the phosphorylated histidine residue and the ATP-binding domain. Analysis of the response regulators predicted that all contain the characteristic amino-terminal receiver domain and a carboxy-terminal DNA-binding domain. Thus, all two-component systems of C. glutamicum appear to have roles in gene regulation.

View larger version (31K): [in this window] [in a new window]   FIG. 1. Maps of the 13 C. glutamicum genome loci containing genes for two-component signal transduction systems. The different colors indicate different functions of the encoded proteins or different localizations of proteins with unknown functions. The numbers are derived from GenBank accession no. BX927147 (13). put., putative; Me, metal; assoc., associated; biosynth., biosynthesis; mech.-sens., mechano-sensitive; CoA, coenzyme A; transp., transporter.

For functional analysis of the two-component systems, deletion mutants, each lacking one pair of sensor kinase/response regulator genes, were constructed using a previously established method (23). In these deletion mutants, only the first six codons of the promoter-proximal gene and the last 12 codons of the promoter-distal gene are still present, whereas the intervening region is replaced by a 21-bp sequence tag. For 12 two-component systems, the corresponding deletion strains could be obtained, showing that these genes are not essential (Table S1 in the supplemental material). In the case of the cgtSR4 genes, however, no deletion mutants were obtained, suggesting that cgtS4 and/or cgtR4 is essential.

Screening of two-component deletion mutants for growth under P_i limitation. In order to determine whether one of the C. glutamicum two-component systems is involved in the phosphate starvation response, the set of 12 deletion mutants was tested for growth in glucose minimal medium containing different P_i concentrations. One deletion strain (cgtRS3 mutant) showed a growth defect under P_i-limiting conditions (0.065 mM) but not under P_i excess (13 mM), indicating that the cgtRS3 genes might have a specific function in the P_i starvation response. When the cgtRS3 mutant was transformed with a plasmid containing the cgtRS3 genes (pEXEx2-phoRS), the growth defect under P_i limitation was abolished and the complemented strain grew almost like the wild type (Fig. 2). This experiment confirmed that the growth phenotype of the mutant was caused by the deletion of the cgtRS3 genes. In this work the corresponding genes were renamed phoR for phosphate response regulator and phoS for phosphate sensor kinase and the mutant was renamed the phoRS mutant. The coding regions of phoR and phoS are separated by 8 bp, suggesting cotranscription of the two genes.

View larger version (11K): [in this window] [in a new window]   FIG. 2. Growth of the C. glutamicum 13032/pEKEx2 (), phoRS/pEKEx2 (), and phoRS/pEKEx2-phoRS (•) strains in CGXII minimum medium with 40 g/liter glucose and 0.065 mM phosphate. Cells were precultured in CGXII medium with 0.065 mM phosphate for 24 h.

Gene expression changes in the phoRS mutant after a shift from P_i-sufficient to P_i-limiting conditions. The growth defect under P_i-limiting conditions indicated that the phoRS mutant is impaired in its ability to induce the phosphate starvation genes (see the introduction). Therefore, the changes in gene expression occurring 10, 30, and 60 min after a shift from P_i excess to P_i starvation were analyzed as described previously for the wild type (12) and as outlined in Materials and Methods. Overall, 34 and 47 genes of the C. glutamicum phoRS mutant showed a 2-fold-increased and a 2-fold-decreased mRNA level, respectively, at least at one time point after the shift (Table 1). Except for the pst genes, none of the prominent phosphate starvation-inducible genes, e.g., the ugpAEBC operon, glpQ1, phoH1, ushA, or nucH, were induced either 10, 30, or 60 min after the shift (Fig. 3B). This is in marked contrast to the situation in the wild type (Fig. 3A) and supports a critical role for the PhoRS two-component system in the phosphate starvation response of C. glutamicum. In the case of the pstSCAB operon, a twofold- to fivefold-increased mRNA level was observed within 1 hour after the shift. Since this induction level is lower than in the wild type, the data suggest also that the pst operon is controlled by the PhoRS system.

View this table: [in this window] [in a new window]   TABLE 1. Genes showing 2-fold-altered mRNA ratios in the C. glutamicum phoRS mutant 10, 30, or 60 min after a shift from Pi excess to Pi limitation

View larger version (26K): [in this window] [in a new window]   FIG. 3. Relative mRNA levels of phosphate starvation-inducible genes in the C. glutamicum wild type (A) and the phoRS strain (B). The ratios represent the mRNA levels 10 min (white bars), 30 min (gray bars), and 60 min (black bars) after the onset of Pi starvation versus the mRNA level immediately before the onset of Pi starvation. The experiment was performed in triplicate, and average mRNA ratios were calculated. The criterion used for selection of RNA ratios was a signal-to-noise ratio of 3 for either Cy3 or Cy5 fluorescence. The data for the wild type (A) were taken from our previous studies (12).

In a second set of DNA microarray experiments, the mRNA levels of the wild type and the phoRS mutant were directly compared before and 60 min after a shift from P_i excess (13 mM) to P_i limitation (0.065 mM). As expected from the previous experiment, the majority of phosphate starvation-inducible genes (pstS, pstA, pstB, ugpA, ugpE, ugpB, ugpC, glpQ1, phoH1, ushA, nucH, pctB, pctC, and cg3393) showed higher mRNA levels in the wild type than in the phoRS mutant after the shift (data not shown).

Primer extension analysis. In order to verify the DNA microarray data and to determine the transcriptional start sites, primer extension analyses were performed for prominent psi genes, i.e., the pstSCAB operon, the upgAEBC operon, the phoRS operon, ushA, and nucH (Fig. 4). In these experiments, RNA of the wild type and of the phoRS mutant grown either under P_i excess (13 mM) or under P_i limitation (0.065 mM) for 10 min (phoRS), 60 min (pst, ugp), or 90 min (ushA, nucH) were used. The growth conditions and the preparation of RNA were identical to those in DNA microarray experiments. In the case of the wild type, primer extension products were observed for all five tested genes with RNA from cells grown under P_i limitation but not with RNA from cells grown under P_i excess, confirming that the expression of these genes is induced under P_i limitation. In the case of the phoRS mutant, primer extension products for ugpA, phoR, ushA, and nucH were observed neither with RNA from cells grown under P_i excess nor with RNA from cells grown under P_i limitation. Again, this confirms the results of the microarray data, which indicate that induction of these genes under P_i starvation is dependent on the PhoRS two-component system. In the case of pstS, a primer extension product was obtained with RNA from phoRS cells grown under P_i limitation but not with RNA from phoRS cells grown under P_i excess. The signal intensity of the pstS primer extension product was lower in the phoRS mutant than in the wild type (Fig. 4A), which is in agreement with the DNA microarray data, where the induction ratio was lower in the phoRS strain than in the wild type. The primer extension data thus support the assumption that induction of the pstSCAB operon by P_i starvation is dependent on PhoRS and one or several additional regulators which are not yet known. The fact that no primer extension product was obtained for phoR with RNA from phoRS cells grown under P_i limitation suggests a positive autoregulation of the phoRS genes.

View larger version (61K): [in this window] [in a new window]   FIG. 4. Comparison of mRNA levels and determination of the transcriptional start sites of the C. glutamicum genes pstS (A), ugpA (B), phoR (C), ushA (D), and nucH (E) by primer extension analysis. The reverse transcriptase reactions were performed with the oligonucleotides pstS_prext2, ugpA_prext2, phoR_prext1b, ushA80prext, and nucH90prext for these four genes, respectively, and 20 µg of total RNA was isolated from the following strains: the wild type grown under phosphate excess (lane 1); the wild type 60 min (A and B), 10 min (C), or 90 min (D and E) after a shift from 13 mM Pi to 0.065 mM Pi (lane 2); the phoRS mutant grown under Pi excess (lane 3); and the phoRS mutant 60 min (A and B), 10 min (C), or 90 min (D and E) after a shift from 13 mM Pi to 0.065 mM Pi (lane 4). The transcriptional start sites are indicated by asterisks. The corresponding sequencing reactions were generated by using the same IRD-800-labeled oligonucleotide as in the primer extension reactions as well as PCR products which cover the region of the respective transcriptional start site as the template DNA. In panel F, the promoter regions derived from the primer extension studies were aligned. The transcriptional start points are shown in bold and underlined, the proposed start codons are indicated in bold, and putative –10 regions are shaded in gray.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

 
In many bacteria whose phosphate starvation response has been studied in some detail, e.g., E. coli, B. subtilis, Synechocystis spp. (32), or Streptomyces spp. (30, 31), two-component signal transduction systems play a central regulatory role in this process. In the work presented here, phenotypic and genetic evidence that this is also the case in C. glutamicum was obtained. Screening of 12 C. glutamicum deletion mutants, each lacking one specific two-component system, revealed that one of them, the phoRS strain, is specifically impaired in its ability to grow under P_i limitation. Complementation showed that the phoRS deletion is responsible for the growth impairment of this strain. Independent evidence for an involvement of the phoRS genes in the P_i starvation response was obtained previously during the elucidation of the phosphate starvation stimulon of C. glutamicum by DNA microarrays (12). In these studies, the mRNA levels of phoS and phoR were strongly increased (fivefold and eightfold, respectively) 10 min after a shift from P_i excess to P_i limitation but reached preshift levels within 60 to 90 min. Of the other 24 genes encoding proteins of two-component systems, only cgtR9 also showed a >4-fold-altered expression after the shift. Its mRNA level was sevenfold increased 30 min after the shift and remained increased up to 120 min after the shift (12). Preliminary results suggest that the CgtSR9 two-component system is involved in copper metabolism, since the cgtSR9 mutant has a lower copper resistance than the wild type (M. Brocker and M. Bott, unpublished data). It therefore seems likely that the CgtSR9 two-component system is responsible for the induction of the genes involved in copper metabolism (subcluster 2) that were identified in the hierarchical cluster analysis (12). Consequently, PhoRS is the only two-component system of C. glutamicum whose synthesis is rapidly and transiently induced in response to a shift to P_i limitation.

Transcriptome comparisons with DNA microarrays of the phoRS mutant before and after a shift from P_i excess to P_i limitation showed that in contrast to what occurs in the wild type, none of the characteristic psi genes except pstSCAB (e.g., ugpAEBC, glpQ1, phoH, ushA, or nucH) was induced within 60 min after the shift (Fig. 3). The results of the microarrays regarding the expression pattern of the pst, ugp, and phoRS genes were confirmed independently by primer extension analyses (Fig. 4), which also provided evidence for positive autoregulation of the phoRS genes. The induction of the pstSCAB genes in the phoRS mutant suggests the presence of one or more additional regulators which are not yet known. A situation where more than one regulator is involved in the phosphate starvation response has also been found in B. subtilis (4).

Bioinformatic analyses revealed that the PhoS and PhoR proteins of C. glutamicum are highly conserved within the suborder Corynebacterineae. The sensor kinase PhoS orthologs from Corynebacterium efficiens (locus tag CE2493), Corynebacterium diphtheriae (DIP1935), Mycobacterium avium subsp. paratuberculosis (MAP0592), and Mycobacterium tuberculosis (Rv0758) possess 73%, 51%, 44%, and 44% sequence identity, respectively. The response regulator PhoR orthologs from C. efficiens (CE494), C. diphtheriae (DIP1936), Mycobacterium avium subsp. paratuberculosis (MAP0591), and Mycobacterium tuberculosis (Rv0757) possess 91%, 81%, 65%, and 65% sequence identity, respectively. However, an involvement of theseorthologs in phosphate regulation has not been shown. The results provided here for C. glutamicum certainly support such a function.

In summary, our results show that 12 of the 13 two-component systems of C. glutamicum are not essential, and they provide clear evidence for an involvement of the C. glutamicum PhoRS two-component system in the adaptation to phosphate-limiting conditions. Our future studies will focus on the identification of the direct target genes and the binding site of the response regulator PhoR.

	ACKNOWLEDGMENTS

 
This work was supported by the German Ministry of Education and Research (BMBF) within the framework of genome research on prokaryotic organisms (GenoMik) through grant 031U113D/031U213D.

We thank Hermann Sahm for continuous support.

	FOOTNOTES

 
* Corresponding author. Mailing address: Institut für Biotechnologie 1, Forschungszentrum Jülich, D-52425 Jülich, Germany. Phone: 49 2461 61 5515. Fax: 49 2461 61 2710. E-mail: m.bott{at}fz-juelich.de.

Supplemental material for this article may be found at http://jb.asm.org/.

These authors contributed equally to this work.

Present address: Project House ProFerm, Degussa AG, D-63457 Hanau, Germany.

	REFERENCES

Top Abstract Introduction Materials and Methods Results Discussion References

 

1. Abe, S., K. Takayama, and S. Kinoshita. 1967. Taxonomical studies on glutamic acid producing bacteria. J. Gen. Appl. Microbiol. 13:279-301.
2. Antelmann, H., C. Scharf, and M. Hecker. 2000. Phosphate starvation-inducible proteins of Bacillus subtilis: proteomics and transcriptional analysis. J. Bacteriol. 182:4478-4490.[Abstract/Free Full Text]
3. Aravind, L., and C. P. Ponting. 1999. The cytoplasmic helical linker domain of receptor histidine kinase and methyl-accepting proteins is common to many prokaryotic signalling proteins. FEMS Microbiol. Lett. 176:111-116.[CrossRef][Medline]
4. Birkey, S. M., W. Liu, X. H. Zhang, M. F. Duggan, and F. M. Hulett. 1998. Pho signal transduction network reveals direct transcriptional regulation of one two-component system by another two-component regulator: Bacillus subtilis PhoP directly regulates production of ResD. Mol. Microbiol. 30:943-953.[CrossRef][Medline]
5. Bott, M., M. Meyer, and P. Dimroth. 1995. Regulation of anaerobic citrate metabolism in Klebsiella pneumoniae. Mol. Microbiol. 18:533-546.[CrossRef][Medline]
6. Eggeling, L., and M. Bott (ed.). 2005. Handbook of Corynebacterium glutamicum. CRC Press, Boca Raton, Fla.
7. Eikmanns, B. J., E. Kleinertz, W. Liebl, and H. Sahm. 1991. A family of Corynebacterium glutamicum/Escherichia coli shuttle vectors for cloning, controlled gene expression, and promoter probing. Gene 102:93-98.[CrossRef][Medline]
8. Engels, S., C. Ludwig, J.-E. Schweitzer, C. Mack, M. Bott, and S. Schaffer. 2005. The transcriptional activator ClgR controls transcription of genes involved in proteolysis and DNA repair in Corynebacterium glutamicum. Mol. Microbiol. 57:576-591.[CrossRef][Medline]
9. Engels, S., J. E. Schweitzer, C. Ludwig, M. Bott, and S. Schaffer. 2004. clpC and clpP1P2 gene expression in Corynebacterium glutamicum is controlled by a regulatory network involving the transcriptional regulators ClgR and HspR as well as the ECF sigma factor ^H. Mol. Microbiol. 52:285-302.[CrossRef][Medline]
10. Gerharz, T., S. Reinelt, S. Kaspar, L. Scapozza, and M. Bott. 2003. Identification of basic amino acid residues important for citrate binding by the periplasmic receptor domain of the sensor kinase CitA. Biochemistry 42:5917-5924.[CrossRef][Medline]
11. Hulett, F. M. 2002. The Pho regulon, p. 193-201. In J. A. Sonenshein and R. M. Losick (ed.), Bacillus subtilis and its closest relatives: from genes to cells. ASM Press, Washington, D.C.
12. Ishige, T., M. Krause, M. Bott, V. F. Wendisch, and H. Sahm. 2003. The phosphate starvation stimulon of Corynebacterium glutamicum determined by DNA microarray analyses. J. Bacteriol. 185:4519-4529.[Abstract/Free Full Text]
13. Kalinowski, J., B. Bathe, D. Bartels, N. Bischoff, M. Bott, A. Burkovski, N. Dusch, L. Eggeling, B. J. Eikmanns, L. Gaigalat, A. Goesmann, M. Hartmann, K. Huthmacher, R. Krämer, B. Linke, A. C. McHardy, F. Meyer, B. Möckel, W. Pfefferle, A. Pühler, D. A. Rey, C. Rückert, O. Rupp, H. Sahm, V. F. Wendisch, I. Wiegrabe, and A. Tauch. 2003. The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins. J. Biotechnol. 104:5-25.[CrossRef][Medline]
14. Kaspar, S., R. Perozzo, S. Reinelt, M. Meyer, K. Pfister, L. Scapozza, and M. Bott. 1999. The periplasmic domain of the histidine autokinase CitA functions as a highly specific citrate receptor. Mol. Microbiol. 33:858-872.[CrossRef][Medline]
15. Keilhauer, C., L. Eggeling, and H. Sahm. 1993. Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon. J. Bacteriol. 175:5595-5603.[Abstract/Free Full Text]
16. Lambert, C., D. Weuster-Botz, R. Weichenhain, E. W. Kreutz, A. A. De Graaf, and S. M. Schoberth. 2002. Monitoring of inorganic polyphosphate dynamics in Corynebacterium glutamicum using a novel oxygen sparger for real time P-31 in vivo NMR. Acta Biotechnol. 22:245-260.[CrossRef]
17. Lange, C., D. Rittmann, V. F. Wendisch, M. Bott, and H. Sahm. 2003. Global expression profiling and physiological characterization of Corynebacterium glutamicum grown in the presence of L-valine. Appl. Environ. Microbiol. 69:2521-2532.[Abstract/Free Full Text]
18. Liebl, W. 2005. Corynebacterium taxonomy, p. 9-34. In L. Eggeling and M. Bott (ed.), Handbook of Corynebacterium glutamicum. CRC Press, Boca Raton, Fla.
19. Link, A. J., D. Phillips, and G. M. Church. 1997. Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J. Bacteriol. 179:6228-6237.[Abstract/Free Full Text]
20. Liu, W., S. Eder, and F. M. Hulett. 1998. Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoPP. J. Bacteriol. 180:753-758.[Abstract/Free Full Text]
21. Menkel, E., G. Thierbach, L. Eggeling, and H. Sahm. 1989. Influence of increased aspartate availability on lysine formation by a recombinant strain of Corynebacterium glutamicum and utilization of fumarate. Appl. Environ. Microbiol. 55:684-688.[Abstract/Free Full Text]
22. Möker, N., M. Brocker, S. Schaffer, R. Krämer, S. Morbach, and M. Bott. 2004. Deletion of the genes encoding the MtrA-MtrB two-component system of Corynebacterium glutamicum has a strong influence on cell morphology, antibiotics susceptibility and expression of genes involved in osmoprotection. Mol. Microbiol. 54:420-438.[CrossRef][Medline]
23. Niebisch, A., and M. Bott. 2001. Molecular analysis of the cytochrome bc₁-aa₃ branch of the Corynebacterium glutamicum respiratory chain containing an unusual diheme cytochrome c₁. Arch. Microbiol. 175:282-294.[CrossRef][Medline]
24. Pallerla, S. R., S. Knebel, T. Polen, P. Klauth, J. Hollender, V. F. Wendisch, and S. M. Schoberth. 2005. Formation of volutin granules in Corynebacterium glutamicum. FEMS Microbiol. Lett. 243:133-140.[CrossRef][Medline]
25. Patek, M., J. Nesvera, A. Guyonvarch, O. Reyes, and G. Leblon. 2003. Promoters of Corynebacterium glutamicum. J. Biotechnol. 104:311-323.[CrossRef][Medline]
26. Reinelt, S., E. Hofmann, T. Gerharz, M. Bott, and D. R. Madden. 2003. The structure of the periplasmic ligand-binding domain of the sensor kinase CitA reveals the first extracellular PAS domain. J. Biol. Chem. 278:39189-39196.[Abstract/Free Full Text]
27. Rittmann, D., U. Sorger-Herrmann, and V. F. Wendisch. 2005. Phosphate starvation-inducible gene ushA encodes a 5' nucleotidase required for growth of Corynebacterium glutamicum on media with nucleotides as the phosphorus source. Appl. Environ. Microbiol. 71:4339-4344.[Abstract/Free Full Text]
28. Schäfer, A., A. Tauch, W. Jäger, J. Kalinowski, G. Thierbach, and A. Pühler. 1994. Small mobilizable multipurpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145:69-73.[CrossRef][Medline]
29. Skerra, A., and T. G. Schmidt. 2000. Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins. Methods Enzymol. 326:271-304.[Medline]
30. Sola-Landa, A., R. S. Moura, and J. F. Martin. 2003. The two-component PhoR-PhoP system controls both primary metabolism and secondary metabolite biosynthesis in Streptomyces lividans. Proc. Natl. Acad. Sci. USA 100:6133-6138.[Abstract/Free Full Text]
31. Sola-Landa, A., A. Rodriguez-Garcia, E. Franco-Dominguez, and J. F. Martin. 2005. Binding of PhoP to promoters of phosphate-regulated genes in Streptomyces coelicolor: identification of PHO boxes. Mol. Microbiol. 56:1373-1385.[CrossRef][Medline]
32. Suzuki, S., A. Ferjani, I. Suzuki, and N. Murata. 2004. The SphS-SphR two component system is the exclusive sensor for the induction of gene expression in response to phosphate limitation in Synechocystis. J. Biol. Chem. 279:13234-13240.[Abstract/Free Full Text]
33. Wanner, B. L. 1996. Phosphorus assimilation and control of the phosphate regulon, p. 1357-1381. In F. C. Neidhardt (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 1. ASM Press, Washington, D.C.
34. Wendisch, V. F., and M. Bott. 2005. Phosphorus metabolism, p. 377-396. In L. Eggeling and M. Bott (ed.), Handbook of Corynebacterium glutamicum. CRC Press, Boca Raton, Fla.

This article has been cited by other articles:

• Fiedler, T., Mix, M., Meyer, U., Mikkat, S., Glocker, M. O., Bahl, H., Fischer, R.-J. (2008). The Two-Component System PhoPR of Clostridium acetobutylicum Is Involved in Phosphate-Dependent Gene Regulation. J. Bacteriol. 190: 6559-6567 [Abstract] [Full Text]  
• Schaaf, S., Bott, M. (2007). Target Genes and DNA-Binding Sites of the Response Regulator PhoR from Corynebacterium glutamicum. J. Bacteriol. 189: 5002-5011 [Abstract] [Full Text]  
• Moker, N., Kramer, J., Unden, G., Kramer, R., Morbach, S. (2007). In Vitro Analysis of the Two-Component System MtrB-MtrA from Corynebacterium glutamicum. J. Bacteriol. 189: 3645-3649 [Abstract] [Full Text]  
• Yukawa, H., Omumasaba, C. A., Nonaka, H., Kos, P., Okai, N., Suzuki, N., Suda, M., Tsuge, Y., Watanabe, J., Ikeda, Y., Vertes, A. A., Inui, M. (2007). Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R. Microbiology 153: 1042-1058 [Abstract] [Full Text]  
• Mascher, T., Helmann, J. D., Unden, G. (2006). Stimulus Perception in Bacterial Signal-Transducing Histidine Kinases. Microbiol. Mol. Biol. Rev. 70: 910-938 [Abstract] [Full Text]  
• Wennerhold, J., Bott, M. (2006). The DtxR Regulon of Corynebacterium glutamicum.. J. Bacteriol. 188: 2907-2918 [Abstract] [Full Text]  

This Article Abstract Full Text (PDF) Supplemental material Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kocan, M. Articles by Bott, M. Search for Related Content PubMed PubMed Citation Articles by Kocan, M. Articles by Bott, M.