This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miethke, M.
Right arrow Articles by Marahiel, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miethke, M.
Right arrow Articles by Marahiel, M. A.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2006, p. 8655-8657, Vol. 188, No. 24
0021-9193/06/$08.00+0     doi:10.1128/JB.01049-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Iron Starvation Triggers the Stringent Response and Induces Amino Acid Biosynthesis for Bacillibactin Production in Bacillus subtilis{triangledown} ,{dagger}

Marcus Miethke,1 Helga Westers,2 Evert-Jan Blom,2 Oscar P. Kuipers,2 and Mohamed A. Marahiel1*

Fachbereich Chemie/Biochemie der Philipps-Universität Marburg, Hans-Meerwein-Str., D-35032 Marburg, Germany,1 Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, the Netherlands2

Received 18 July 2006/ Accepted 20 September 2006


arrow
ABSTRACT
 
Iron deprivation in bacteria causes the derepression of genes controlled by the ferric uptake regulator (Fur). The present microarray analysis of iron-starved Bacillus subtilis cells grown in minimal medium unveils additional physiological effects on a large number of genes linked to stringent-response regulation and to genes involved in amino acid biosynthesis associated with pathways essential for bacillibactin production.


arrow
TEXT
 
Iron is an essential cofactor in various biosynthetic and bioenergetic pathways. In many bacteria, the Fur-dependent derepression of iron acquisition genes is a common strategy used to overcome iron starvation (13). The Fur regulon of B. subtilis comprises 39 genes coding mainly for siderophore biosynthesis and several iron transporters (1). The Escherichia coli Fur protein controls an even larger regulon of similar constitution and was shown to regulate the cellular iron-protein content (17). This study investigated the consequences of permanent iron depletion under nonrich growth conditions on global gene expression and shows a complex physiological response beyond Fur regulation.

For our analyses, Bacillus subtilis strain ATCC 21332 (sfp+) (7) was grown in a defined medium without (iron-depleted cultures) or with (iron-replete cultures) the addition of 10 µM FeSO4 as described previously (18). The growth rates of iron-depleted and iron-replete cultures were similar until the late exponential phase. The growth of the iron-depleted culture then declined and, in contrast to that of the iron-replete culture, catechol siderophore secretion greatly increased (see Fig. S1 in the supplemental material), confirming iron as the limiting nutrient in the late exponential phase. At this time point (optical density at 600 nm, ~0.35), the mRNA populations of iron-depleted and iron-replete cultures were compared by microarray analysis (essentially performed as described previously [15; see also technical details in the supplemental material]). Genes of several prominent functional and regulatory classes exhibited iron-dependent repression or induction (see Tables S1 and S2 in the supplemental material). As expected, iron starvation led to induction of the Fur regulon, although there were slight differences from previous studies of Fur regulation carried out with B. subtilis 168 (sfp0) in broth medium (1). Interestingly, among the Fur-dependent ABC transporters, the feuABC genes for ferri-bacillibactin uptake (18, 20) showed the strongest induction. In contrast, the fhuBGC-fhuD genes for ferrichrome uptake (24) were only slightly induced and the yfmCDEF and yfiYZ-yfhA ferric iron transporter genes were not induced. This might indicate a hierarchical expression of iron transporters when dominant iron chelators such as bacillibactin are present. Additionally, 24 genes were upregulated that were reported to be regulated by the transcriptional repressor CodY (19). It has been suggested that derepression of GTP-activated CodY is mediated by a decreasing cellular GTP pool upon RelA-dependent formation of (p)ppGpp, the second messenger of the stringent response (21). To investigate this relationship more closely, we compared our transcriptome data with a global stringent-response analysis (8). Indeed, there was a high coincidence of gene regulation between the two studies. Among the genes earlier described to be either positively or negatively RelA regulated, we found 19 and 28 genes to be up- and downregulated during iron starvation, respectively. Furthermore, among the genes that were reported to be affected independently of RelA during the stringent response, we found 26 genes showing a similar iron-dependent repression or induction. These genes mainly belonged to the functional categories of amino acid, purine, and pyrimidine biosynthesis. To confirm these and further results of the microarray analysis, iron-dependent repression or induction of selected genes was subsequently compared by an independent dot blot analysis (Fig. 1; see technical details in the supplemental material). The specific transcript detection showed the same expression pattern for both the RelA-dependent genes rpsP and ald and the CodY-dependent genes lpdV and yurO, as revealed by the transcriptome analysis.


Figure 1
View larger version (26K):
[in this window]
[in a new window]

  		FIG. 1. Dot blot analysis of selected genes. Shown are the relative transcriptional levels of cells cultured in minimal medium under iron-starved (–) or iron-replete (+) conditions. Panels: A, Fur-regulated genes (as controls); B, amino acid biosynthesis genes of the threonine (yclM, hom), serine/glycine (yoaD), and glutamate (gltA) pathways; C, stringent-response-regulated genes; D, CodY-regulated genes; E, genes of the tricarboxylic acid cycle. The arrows indicate increased ({uparrow}), decreased ({downarrow}), or equal (->) transcript amounts that were detected during iron starvation in comparison with iron-replete conditions.

Several enzymes in amino acid biosynthesis pathways are iron dependent, and iron limitation may subsequently cause amino acid starvation. The most abundant amino acid in both gram-positive and gram-negative bacteria is glutamate. Especially Bacillus spp. need a large intracellular glutamate pool (40 to >100 mM) for vegetative growth and adaptational processes (25). Since B. subtilis lacks an anabolic glutamate dehydrogenase (3), glutamate synthesis is strictly iron dependent as iron is needed to assemble the iron-sulfur cluster bound to glutamate synthase as a cofactor (26). The gltA and gltB genes coding for the large and small chains of the B. subtilis glutamate synthase, respectively, were downregulated during iron depletion (see also Fig. 1). The expression of the gltAB operon, which is triple regulated by TnrA (4), GltC (6), and CcpA (9), depends on a sufficient supply of ammonium and glucose (5, 28). Since both ammonium and glucose were present at nonlimiting concentrations in the minimal medium used, the underexpression of gltAB seems to be a direct result of low iron availability. Furthermore, we found that the citB gene coding for the iron-dependent B. subtilis aconitase involved in substrate supply for GltAB was also downregulated by iron depletion, as observed in previous work (1). citB repression seems to be directly iron dependent, since further genes of the tricarboxylic acid cycle coding for non-iron-dependent enzymes were not affected, such as citZ, which is regulated by the same carbon and nitrogen sources as citB (see also Fig. 1) (14, 22). Altogether, gltAB and citB downregulation indicates an iron-dependent bottleneck of glutamate synthesis. A recent study demonstrating SpoT-dependent (p)ppGpp accumulation during iron limitation in E. coli (27) might indicate similar effects in gram-negative bacteria. However, the stringent response observed in B. subtilis during iron starvation is most likely the consequence of deficiencies in iron-dependent amino acid biosynthesis.

Recently, ferri-bacillibactin was shown to be the major endogenously derived iron source of B. subtilis during iron starvation (18). The utilization of this nonribosomally produced catecholic trilactone (2,3-dihydroxybenzoate-glycine-threonine)3 siderophore is mediated by the Fur-regulated FeuABC uptake system and the BesA trilactone hydrolase (18, 20). While the dhbACEBF operon coding for 2,3-dihydroxybenzoate synthesis (23) and bacillibactin assembly (16) is also controlled by Fur (1), iron-dependent regulation of primary metabolic genes involved in bacillibactin synthesis was not reported. In total, our transcriptome study revealed 11 amino acid biosynthesis genes that were more than 40% upregulated during iron starvation. Strikingly, all of them code for enzymes that are essential for the synthesis of the bacillibactin precursors threonine and glycine, as shown schematically in Fig. 2. Threonine synthesis starting from aspartate needs five enzymatic activities. Five genes coding for four of these activities were upregulated: lysC (aspartokinase II) and yclM (aspartokinase III, thrD), encoding two isozymes for the initial reaction (2, 10), as well as hom, thrB, and thrC, coding for homoserine dehydrogenase, homoserine kinase, and threonine synthase, respectively. In the synthesis pathway leading from 3-phosphoglycerate via serine to glycine, the yoaD gene, coding for a putative paralog of the initial enzyme SerA, was upregulated. The genes yclM, hom, and yoaD were selected for dot blot analysis (Fig. 1). In the amino acid biosynthesis network, the threonine, serine/glycine, and cysteine/methionine pathways are interdependent. In total, there are seven specific enzymatic activities needed for cysteine/methionine synthesis. The genes yjcI, yjcJ, yitJ (12), and cysE, coding for four of these activities, were upregulated. Additionally, yxjG, coding for a protein similar to the methionine synthase MetE, possibly provides a further activity to this pathway. Altogether, 9 out of the 11 genes are either S box (yjcI, yjcJ, yitJ, yoaD, and yxjG) or T box (hom, thrB, thrC, and cysE) regulated (11, 12). Furthermore, yjcI, yjcJ, yitJ, and yoaD were shown to be induced RelA independently during the stringent response (8). Thus, in addition to these regulatory mechanisms, it is tempting to speculate that there could be a specific link between iron starvation and/or bacillibactin synthesis and the threonine, serine/glycine, and cysteine/methionine pathways. However, because of the moderate induction of the precursor biosynthesis genes, a more obvious explanation might be the occurrence of S- and T-box-dependent feedback regulation(s) caused by the consumption of threonine, glycine, and serine (as glycine precursor) in bacillibactin synthesis, thus leaving a "regulatory footprint" in the primary metabolism. However, this is the first time that siderophore synthesis-dependent regulation in the primary metabolism was observed, underlining the importance of bacillibactin as a major iron deficiency rescue system in B. subtilis.


Figure 2
View larger version (47K):
[in this window]
[in a new window]

  		FIG. 2. Scheme of bacillibactin synthesis. The bacillibactin precursors glycine and threonine derive from the primary metabolism. The synthesis of 2,3-dihydroxybenzoate (DHB) and the nonribosomally catalyzed bacillibactin assembly are Fur regulated in the secondary metabolism. The numbers shown indicate the numbers of genes that were upregulated during iron starvation. The total numbers of activities involved in the presented pathway sections are in parentheses. reg., regulation.

In conclusion, the results of this study show the relevance of both culture medium composition and the capability of siderophore production to global gene expression during iron starvation and establish novel iron-dependent functional and regulatory connections between differentially classified genes.


arrow
ACKNOWLEDGMENTS
 
We thank the group of E. Bremer (Marburg) for help with RNA preparation and chemiluminescence detection. Anne de Jong, Siger Holsappel, and Anne Sadewasser are acknowledged for technical assistance.

This work was supported by EC grant LSHG-CT-2004-503468.


arrow
FOOTNOTES
 
* Corresponding author. Mailing address: Philipps-Universität Marburg, FB Chemie-Biochemie, Hans-Meerwein-Str., D-35032 Marburg, Germany. Phone: 49 6421 282 5722. Fax: 49 6421 282 2191. E-mail: marahiel{at}chemie.uni-marburg.de. Back

{triangledown} Published ahead of print on 29 September 2006. Back

{dagger} Supplemental material for this article may be found at http://jb.asm.org/. Back


arrow
REFERENCES
 
    1
  1. Baichoo, N., T. Wang, R. Ye, and J. D. Helmann. 2002. Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon. Mol. Microbiol. 45:1613-1629.[CrossRef][Medline]
    2. 2
  2. Belitsky, B. R. 2002. Biosynthesis of amino acids of the glutamate and aspartate families, alanine, and polyamines, p. 203-231. In A. L. Sonenshein (ed.), Bacillus subtilis and its closest relatives. ASM Press, Washington, D.C.
    3. 3
  3. Belitsky, B. R., and A. L. Sonenshein. 1998. Role and regulation of Bacillus subtilis glutamate dehydrogenase genes. J. Bacteriol. 180:6298-6305.[Abstract/Free Full Text]
    4. 4
  4. Belitsky, B. R., L. V. Wray, Jr., S. H. Fisher, D. E. Bohannon, and A. L. Sonenshein. 2000. Role of TnrA in nitrogen source-dependent repression of Bacillus subtilis glutamate synthase gene expression. J. Bacteriol. 182:5939-5947.[Abstract/Free Full Text]
    5. 5
  5. Bohannon, D. E., M. S. Rosenkrantz, and A. L. Sonenshein. 1985. Regulation of Bacillus subtilis glutamate synthase genes by the nitrogen source. J. Bacteriol. 163:957-964.[Abstract/Free Full Text]
    6. 6
  6. Bohannon, D. E., and A. L. Sonenshein. 1989. Positive regulation of glutamate biosynthesis in Bacillus subtilis. J. Bacteriol. 171:4718-4727.[Abstract/Free Full Text]
    7. 7
  7. Cooper, D. G., C. R. Macdonald, S. J. Duff, and N. Kosaric. 1981. Enhanced production of surfactin from Bacillus subtilis by continuous product removal and metal cation additions. Appl. Environ. Microbiol. 42:408-412.[Abstract/Free Full Text]
    8. 8
  8. Eymann, C., G. Homuth, C. Scharf, and M. Hecker. 2002. Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis. J. Bacteriol. 184:2500-2520.[Abstract/Free Full Text]
    9. 9
  9. Faires, N., S. Tobisch, S. Bachem, I. Martin-Verstraete, M. Hecker, and J. Stülke. 1999. The catabolite control protein CcpA controls ammonium assimilation in Bacillus subtilis. J. Mol. Microbiol. Biotechnol. 1:141-148.[Medline]
    10. 10
  10. Graves, L. M., and R. L. Switzer. 1990. Aspartokinase III, a new isozyme in Bacillus subtilis 168. J. Bacteriol. 172:218-223.[Abstract/Free Full Text]
    11. 11
  11. Grundy, F. J., and T. M. Henkin. 1998. The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria. Mol. Microbiol. 30:737-749.[CrossRef][Medline]
    12. 12
  12. Grundy, F. J., and T. M. Henkin. 2002. Synthesis of serine, glycine, cysteine, and methionine, p. 245-254. In A. L. Sonenshein (ed.), Bacillus subtilis and its closest relatives. ASM Press, Washington, D.C.
    13. 13
  13. Hantke, K. 2001. Iron and metal regulation in bacteria. Curr. Opin. Microbiol. 4:172-177.[CrossRef][Medline]
    14. 14
  14. Jourlin-Castelli, C., N. Mani, M. M. Nakano, and A. L. Sonenshein. 2000. CcpC, a novel regulator of the LysR family required for glucose repression of the citB gene in Bacillus subtilis. J. Mol. Biol. 295:865-878.[CrossRef][Medline]
    15. 15
  15. Lulko, A. T., G. Buist, J. Kok, and O. P. Kuipers. Transcriptome analysis of temporal regulation of carbon-metabolism by CcpA in Bacillus subtilis. J. Mol. Biol. Biotechnol., in press.
    16. 16
  16. May, J. J., T. M. Wendrich, and M. A. Marahiel. 2001. The dhb operon of Bacillus subtilis encodes the biosynthetic template for the catecholic siderophore 2,3-dihydroxybenzoate-glycine-threonine trimeric ester bacillibactin. J. Biol. Chem. 276:7209-7217.[Abstract/Free Full Text]
    17. 17
  17. McHugh, J. P., F. Rodriguez-Quinones, H. Abdul-Tehrani, D. A. Svistunenko, R. K. Poole, C. E. Cooper, and S. C. Andrews. 2003. Global iron-dependent gene regulation in Escherichia coli. A new mechanism for iron homeostasis. J. Biol. Chem. 278:29478-29486.[Abstract/Free Full Text]
    18. 18
  18. Miethke, M., O. Klotz, U. Linne, J. J. May, C. L. Beckering, and M. A. Marahiel. 2006. Ferri-bacillibactin uptake and hydrolysis in Bacillus subtilis. Mol. Microbiol. 61:1413-1427.[CrossRef][Medline]
    19. 19
  19. Molle, V., Y. Nakaura, R. P. Shivers, H. Yamaguchi, R. Losick, Y. Fujita, and A. L. Sonenshein. 2003. Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis. J. Bacteriol. 185:1911-1922.[Abstract/Free Full Text]
    20. 20
  20. Ollinger, J., K. B. Song, H. Antelmann, M. Hecker, and J. D. Helmann. 2006. Role of the Fur regulon in iron transport in Bacillus subtilis. J. Bacteriol. 188:3664-3673.[Abstract/Free Full Text]
    21. 21
  21. Ratnayake-Lecamwasam, M., P. Serror, K. W. Wong, and A. L. Sonenshein. 2001. Bacillus subtilis CodY represses early-stationary-phase genes by sensing GTP levels. Genes Dev. 15:1093-1103.[Abstract/Free Full Text]
    22. 22
  22. Rosenkrantz, M. S., D. W. Dingman, and A. L. Sonenshein. 1985. Bacillus subtilis citB gene is regulated synergistically by glucose and glutamine. J. Bacteriol. 164:155-164.[Abstract/Free Full Text]
    23. 23
  23. Rowland, B. M., T. H. Grossman, M. S. Osburne, and H. W. Taber. 1996. Sequence and genetic organization of a Bacillus subtilis operon encoding 2,3-dihydroxybenzoate biosynthetic enzymes. Gene 178:119-123.[CrossRef][Medline]
    24. 24
  24. Schneider, R., and K. Hantke. 1993. Iron-hydroxamate uptake systems in Bacillus subtilis: identification of a lipoprotein as part of a binding protein-dependent transport system. Mol. Microbiol. 8:111-121.[Medline]
    25. 25
  25. Schreier, H. J. 1993. Biosynthesis of glutamine and glutamate and the assimilation of ammonia, p. 281-298. In A. L. Sonenshein (ed.), Bacillus subtilis and other gram-positive bacteria. American Society for Microbiology, Washington, D.C.
    26. 26
  26. van den Heuvel, R. H., D. Ferrari, R. T. Bossi, S. Ravasio, B. Curti, M. A. Vanoni, F. J. Florencio, and A. Mattevi. 2002. Structural studies on the synchronization of catalytic centers in glutamate synthase. J. Biol. Chem. 277:24579-24583.[Abstract/Free Full Text]
    27. 27
  27. Vinella, D., C. Albrecht, M. Cashel, and R. D'Ari. 2005. Iron limitation induces SpoT-dependent accumulation of ppGpp in Escherichia coli. Mol. Microbiol. 56:958-970.[CrossRef][Medline]
    28. 28
  28. Wacker, I., H. Ludwig, I. Reif, H. M. Blencke, C. Detsch, and J. Stülke. 2003. The regulatory link between carbon and nitrogen metabolism in Bacillus subtilis: regulation of the gltAB operon by the catabolite control protein CcpA. Microbiology 149:3001-3009.[Abstract/Free Full Text]

Journal of Bacteriology, December 2006, p. 8655-8657, Vol. 188, No. 24
0021-9193/06/$08.00+0     doi:10.1128/JB.01049-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gaballa, A., Antelmann, H., Aguilar, C., Khakh, S. K., Song, K.-B., Smaldone, G. T., Helmann, J. D. (2008). The Bacillus subtilis iron-sparing response is mediated by a Fur-regulated small RNA and three small, basic proteins. Proc. Natl. Acad. Sci. USA 105: 11927-11932 [Abstract] [Full Text]  
  • Chavez de Paz, L. E., Hamilton, I. R., Svensater, G. (2008). Oral bacteria in biofilms exhibit slow reactivation from nutrient deprivation. Microbiology 154: 1927-1938 [Abstract] [Full Text]  
  • Balado, M., Osorio, C. R., Lemos, M. L. (2008). Biosynthetic and regulatory elements involved in the production of the siderophore vanchrobactin in Vibrio anguillarum. Microbiology 154: 1400-1413 [Abstract] [Full Text]  

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miethke, M.
Right arrow Articles by Marahiel, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miethke, M.
Right arrow Articles by Marahiel, M. A.

Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»