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Journal of Bacteriology, January 2007, p. 363-368, Vol. 189, No. 2
0021-9193/07/$08.00+0     doi:10.1128/JB.01344-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Minimum Length Requirement of the Flexible N-Terminal Translocation Subdomain of Colicin E3

Onkar Sharma and William A. Cramer^*

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907

Received 23 August 2006/ Accepted 20 October 2006

Top Abstract Introduction Materials and Methods Results Discussion References

 
The 315-residue N-terminal T domain of colicin E3 functions in translocation of the colicin across the outer membrane through its interaction with outer membrane proteins including the OmpF porin. The first 83 residues of the T domain are known from structure studies to be disordered. This flexible translocation subdomain contains the TolB box (residues 34 to 46) that must cross the outer membrane in an early translocation event, allowing the colicin to bind to the TolB protein in the periplasm. In the present study, it was found that cytotoxicity of the colicin requires a minimum length of 19 to 23 residues between the C terminus (residue 46) of the TolB box and the end of the flexible subdomain (residue 83). Colicin E3 molecules of sufficient length display normal binding to TolB and occlusion of OmpF channels in vitro. The length of the N-terminal subdomain is critical because it allows the TolB box to cross the outer membrane and interact with TolB. It is proposed that the length constraint is a consequence of ordered structure in the downstream segment of the T domain (residues 84 to 315) that prevents its insertion through the outer membrane via a translocation pore that includes OmpF.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Transport of proteins across membranes in import and secretion is known to require an assembly of integral membrane proteins that provides passage across the hydrophobic membrane (29, 36). Such an assembly is utilized by colicins, which parasitize receptors in the outer membrane to gain entry into the cell (8, 27, 28, 46). Colicins are highly specific in that they kill only those cells that (i) contain the receptor protein to which colicin binds and (ii) do not produce the cognate immunity protein, providing a selective advantage to the colicin-producing cell in a competitive environment (37).

Colicin production is under SOS control, forming part of the stress response of the cell (20, 43). Colicins are produced in complex with a small (molecular mass of 10 kDa) immunity protein that protects the cell from both exogenous and endogenous colicins (7, 22). Colicins have been divided into two groups based on the protein translocation network utilized to cross the outer membrane. Group A colicins, such as the E colicins, utilize the Tol system, consisting of TolA, TolB, TolQ, TolR, and Pal proteins (15, 28, 31). Group B colicins, such as colicins B, D, Ia, and Ib, utilize the Ton system consisting of TonB, ExbB, and ExbD proteins (8, 16). Colicin E3, a 16S rRNase (5), possesses a domain structure (34, 35, 41) as found in other colicins (1, 2, 13, 14, 45). It consists of three separate domains that have distinct roles. Its 315-residue N-terminal T domain functions in cell translocation and entry (1, 2, 41). The central 135-residue receptor (R) domain binds to BtuB (25), and the C-terminal domain contains the catalytic activity (34, 35, 41). The first step of E3 entry into the cell involves binding of the R domain to BtuB (25), a 22-stranded ß-barrel outer membrane receptor whose metabolic function is transport of cyanocobalamin (vitamin B₁₂) across the outer membrane (10, 17, 24). It has been inferred that colicin is translocated across the outer membrane through OmpF (21, 26, 47), a very abundant (10⁵ copies/cell) (33) outer membrane porin (12, 32), and binds to TolB protein in the periplasmic space (6). However, the details of colicin entry through OmpF are largely unknown. The cytotoxicity of colicin E3 is dependent on the interaction of the periplasmic TolB protein with the TolB box spanning residues 34 to 46 (18, 19), which is contained in the 83-residue N-terminal translocation subdomain—the T83 segment. It is likely that the high Gly content of this subdomain and its resulting flexibility (11, 41) allow it to traverse the outer membrane through a translocation complex that contains OmpF (25, 47) and to interact with the TolB protein in the periplasmic space (6).

The present studies show that the peptide segment (TolB extensor) that separates the C-terminal end of the TolB box, residue 46, from the end of the N-terminal flexible subdomain, residue 83, has a minimum length. The length of the segment is 37 residues (residue 47 to 83) in the wild-type protein. However, the minimum length of this TolB extensor that is required for cytotoxic activity is between 19 and 23 residues. It is proposed that this length constraint results from the existence of a folded conformational state of the downstream region (84 to 315) of the T domain that blocks its transfer across the outer membrane through the translocation pore (Fig. 1A and B).

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FIG. 1. Models for different conformational (folding) states of colicin E3 when it binds to, and inserts into, a translocation pore that utilizes OmpF. (A) T domain is fully/partially folded when colicin interacts with the TolB protein. The immunity protein may or may not be bound to colicin E3 when it interacts with TolB protein. In this case, the TolB extensor region (37 residues), extending from residue 46 to 83, has no extra length, thus causing steric limitations reflected in the requirement for a minimum length of the TolB extensor. The R domain is depicted to be folded as a coiled coil. (B) Colicin/T domain is unfolded when it interacts with TolB. In this case, there is extra length in the TolB extensor. The coiled coil of the R domain is depicted as partly unfolded. (C) Outer membrane BtuB/OmpF translocon for the import of colicin E3. Abbreviations: C, catalytic domain; R, receptor binding domain; T, translocation domain; Imm, immunity protein; OM, outer membrane; PGL, peptidoglycan layer.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Bacterial strains, media, and plasmids. Escherichia coli XL1-Blue strain was used as the host strain for cloning of mutations and deletions. All cloning was done in the pET41b vector, with the colicin constructs containing a His₈ tag at the C terminus of the immunity protein. E. coli BL21(DE3) was the host strain for the expression vector pET41b. In the pET41b vector, protein expression is under the control of a strong isopropyl-ß-D-thiogalactopyranoside (IPTG)-inducible T7 RNA polymerase promoter. All cultures were grown in LB media or on LB agar plates supplemented with antibiotic when required.

Mutagenesis and protein purification. All cloning and mutagenesis was done using standard protocols. Colicin E3 was first amplified using upstream (Nde primer) and downstream (Xho primer) primers and then cloned between the NdeI-XhoI sites of the expression vector pET41b, so that ImmE3 contained a C-terminal polyhistidine tag to facilitate protein purification (pWC41E3). All mutagenesis experiments were carried out using this construct by an overlap two-step PCR method (38). The first and second PCR were set up, respectively, using (i) the mutation containing primers and the corresponding upstream (Nde) or downstream (Xho) primer or (ii) the Nde/Xho primers and the purified products of the first PCR as the template. This PCR product was then digested with NdeI-XhoI and cloned in pET41b. All constructs contained a C-terminal His₈ tag on ImmE3. Purification of overexpressed colicin E3 constructs was carried out with a Ni-charged iminodiacetic acid-agarose column. The colicin E3 deletion constructs generated for the present study are E3 65-73 and E3 72-80, with 9 residues deleted; a 14-residue deletion construct, E3 65-78; and E3 65-83, with 19 residues missing from the TolB extensor region. Further, E3 60-64/65-78, with 14 residues deleted and residues 60 to 64 mutated (S60A, G61S, H62A, G63S, N64A) was created. Also, 5 residues were added to E3 65-83 to generate the construct E3 79-83/65-78 which can also be considered a construct with 65 to 78 residues deleted and residues 79 to 83 mutated (G79S, N80A, L81A, S82A, A83S).

Plasmid pRJ379 containing C-terminally His-tagged TolB in the pET21d vector (9) was a generous gift from R. James and was used for the overexpression and purification of TolB. Purification of TolB was done by metal affinity chromatography using an Ni-charged iminodiacetic acid-agarose column.

Cytotoxicity. Cytotoxicity of the different colicin E3 constructs was determined by analyzing the effect of colicin constructs on the growth rate of colicin-sensitive E. coli K17 indicator cells. One hundred milliliters of LB media was inoculated with an overnight culture of the indicator cells and grown to an OD₆₀₀ (optical density at 600 nm) of 0.1. The culture was then aliquoted, and a different colicin construct was added to each sample at a concentration of 3.2 nM. Cell growth was monitored by measuring the OD₆₀₀ at 1-h intervals.

Cytotoxicity of the E3 constructs was also compared by analysis of colony-forming ability. E. coli K17 cells were grown at 37°C under aerobic shaking conditions to an OD₆₀₀ of 0.1, when 3.2 nM colicin was added. Cells were incubated for 1 h and then diluted and transferred to LB agar plates, from which the relative colony-forming ability was measured. The effective multiplicity (m) was calculated from the relative colony-forming ability that resulted from the cytotoxic action of each colicin construct, assuming exponential loss of the surviving cell population (S) relative to the untreated control cells (S_o) as a function of added colicin (i.e., S/S_o = e^–m). It is recognized that cell survival is not truly an exponential process at low levels of cell survival. Thus, the multiplicity, m, is used as a qualitative measure of activity of the different constructs.

TolB binding. TolB binding to the various colicin constructs was analyzed by gel filtration chromatography, using the different elution patterns of E3 and TolB proteins on a Superdex 75 column (total column volume = 23.6 ml). Each colicin construct was mixed with TolB protein in a 1:1 molar ratio (18 µM), and the binding reaction was allowed to proceed for 10 min. This was followed by separation on the Superdex 75 column and determination of the protein peaks in the elution profile. The total protein in each peak, represented by the area under that peak, was calculated for each colicin construct.

Occlusion of OmpF. The E3 constructs were used to study occlusion of OmpF channels incorporated into planar bilayers as described previously (47). The synthetic phospholipids DOPC and DOPE were mixed in a 1:1 molar (total concentration, 10 mg/ml) ratio, dissolved in n-decane, and then used to form planar bilayers (4, 42). Bilayers were formed by painting lipids using a brush technique (30) on a 0.45-mm aperture in a partition separating two 1-ml chambers containing 10 mM HEPES (pH 7.0), 0.1 M KCl at 23°C. Occlusion was studied by measuring the current across this aperture with Ag/AgCl electrodes which are immersed into the two chambers, using a Warner BC-525C amplifier (Hamden, CT). To form channels, OmpF (1 pg/ml to 1 ng/ml) was added to the cis side of the membrane, and the solution was stirred until channels appeared. A transmembrane potential was applied to the cis side, with the trans side at electrical ground. A final concentration of 0.5 to 1 µg/ml of the colicin construct whose occlusion activity was to be tested was added to the trans or cis side of the aperture.

Top Abstract Introduction Materials and Methods Results Discussion References

 
TolB binding. To specifically study the role of the length of the TolB extensor region (segment 47 to 83) in colicin cytotoxicity, it was necessary to make deletions that would not affect the affinity of colicin E3 for TolB. Otherwise, any decrease in cytotoxicity might be ambiguous, as it would include effects of both length and TolB binding affinity. Therefore, deletions in the TolB extensor were made as far downstream from the TolB box as possible. A colicin construct was first allowed to interact with TolB, following which the complex was separated on a Superdex 75 column. The peak fractions of colicin E3 (and constructs) and TolB protein appeared, respectively, at elution volumes of 8.2 ± 0.3 ml and 11.0 ± 0.1 ml. The colicin/TolB complex eluted at a volume of 8.0 ± 0.35 ml. Because the peak of the colicin-TolB complex overlapped that of the colicin alone, the amount of free TolB (area under of curve of TolB elution peak) was used to measure the TolB binding affinity of the colicin. Deletion of 14 (65-78) or 19 (65-83) residues from the TolB extensor region did not affect binding to TolB. Two additional constructs were tested that were important in the cytotoxicity studies (see below). Mutation of 5 residues, N- and C-terminal to the 65-78 deletion, in the two mutants, E3 60-64/65-78 and E3 79-83/65-78, did not affect binding to TolB. All of the deletion constructs except E3 43-52 displayed wild-type affinity for TolB (Fig. 2). There was almost no binding of E3 43-52 to TolB, which is expected, as this construct lacks the complete TolB box that spans residues 34 to 46 (19). The Trp39Ala (W39A) mutant was a negative control, as modifications of the TolB box prevent binding to TolB (9). Studies on the question of a critical length of the N-terminal domain were confined to the segment spanning residues 60 to 80 because deletions/mutations in this region did not affect TolB binding.

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FIG. 2. TolB binding affinity of colicin constructs measured by the area under the elution peaks formed by colicin/TolB complex separated on a Superdex 75 column, as described in Materials and Methods. The affinity is normalized to the total amount of TolB added (1.0 on the ordinate).

Cytotoxicity. Cytotoxicity, used as an indicator of the relative activity of the constructs obtained from mutations in the colicin N-terminal subdomain, was assayed through the effect of colicin on the growth of sensitive cells (Fig. 3A and B) and the relative colony forming ability (Fig. 3C). Growth curves of wild-type cells to which no colicin or colicin E3 (3.2 nM) was added are compared (Fig. 3A). Deletion of 29 residues (E3 55-83) or 19 residues (E3 65-83) abolished cytotoxicity so that the growth curve was the same as the control to which no colicin was added (Fig. 3A). However, deletion of 9 residues (E3 65-73 and E3 72-80) at either of the two positions in the extensor region had a negligible effect on colicin cytotoxicity (Fig. 3A). Increasing the length of the deletion to 14 residues (E3 65-78) resulted in a small decrease (7% decrease in the calculated multiplicity, m) in cytotoxicity compared to the wild-type colicin, as shown in the comparison of growth curve inhibition (Fig. 3B) and the effective multiplicities (Fig. 3C). Addition of 5 residues to the 19 residue deletion construct E3 65-83, to generate a 14 residue deletion construct E3 79-83/65-78, restored essentially all activity compared with E3 65-78, which also has 14 residues deleted in the TolB extensor region (Fig. 3B and C). The 5 residues (Ser, Ala, Ala, Ala, Ser), added to the C-terminal end of the 65-83 deletion, are different from the residues present in the wild-type E3 (Gly, Asn, Leu, Ser, Ala). Thus, because deleting 19 residues from the 37-residue extensor abolishes all activity and removal of 14 residues allows almost full cytotoxicity, the minimum length of the TolB extensor is 19 to 23 residues. Deletions that further shorten this segment abolish colicin cytotoxicity.

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FIG. 3. Effect of the various colicin constructs on the growth of E. coli K17 indicator cells. (A) Colicins were added at a concentration of 3.2 nM. E3 55-83, E3 65-83, and E3 43-52 did not inhibit cell growth, while E3 65-73 and E3 72-80 were as active as wild-type E3. (B) E3 65-78 and E3 79-83/65-78 had a similar activity, which was slightly less than that of wild-type E3. E3 60-64/65-78 showed decreased cytotoxicity compared to E3 65-78. Error bars are contained within symbols if they are not explicitly displayed. (C) Effective multiplicity of colicin and constructs that inhibited cell growth in panel B. *, The m value and fractional survival for E3 65-83 have been assumed to be similar to the control in which no colicin was added on the basis of their identical growth curves and is shown here for purposes of comparison.

OmpF binding site. A binding site for OmpF has been proposed in the region 60 to 80 of colicin E9 (21). No such binding site was found between residues 65 to 83 of colicin E3 because deletions or mutations in this region did not affect colicin cytotoxicity. However, when residues 60 to 64 were mutated (S60A, G61S, H62A, G63S, N64A) in the construct E3 60-64/65-78, there was a decrease of 40% in the effective multiplicity, m, compared to colicin E3 65-78 and E3 79-83/65-78 (Fig. 3B and C). Thus, it cannot be excluded that residues 60 to 64 could participate in binding to OmpF.

Occlusion of OmpF channels. To further characterize the role of residues 60 to 80, E3 deletion constructs were also tested for their ability to occlude ion channels of OmpF porin incorporated in planar bilayer membranes. Previously, it has been shown that colicin E3 (but not E1), added from the trans side of the membrane, is able to specifically occlude OmpF channels on application of cis-negative transmembrane potential (47). The occlusion effect was negated when the transmembrane potential was changed to cis positive. Residues 5 and/or 7 were shown to be essential for occlusion of OmpF (47), and the colicin-OmpF interaction is almost abolished (reduced by 85%) when the first 7 residues are deleted (21). The ability of the different E3 constructs to occlude OmpF is summarized in Fig. 4C. E3 55-83, in which most of the TolB extensor is deleted, was able to occlude OmpF when added from the trans side but not from the cis side (Fig. 4), as was observed for wild-type E3. Thus, the interaction of residues 60 to 80 with OmpF is weak or negligible. The occlusion of OmpF is dependent on residues 5 and/or 7, near the N terminus of the 83-residue translocation subdomain, rather than those in the segment of residues 60 to 80 near its C terminus.

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FIG. 4. E3 55-83 occludes OmpF channels in planar bilayers. OmpF was added to the cis side of the membrane and the solution stirred until channels appeared. The transmembrane current was recorded upon application of transmembrane potentials, 50mV and –50mV (sign of the potential is that of cis side). (A) E3 55-83 was added to the cis side, and no occlusion was seen upon application of potential. (B) When the colicin was added from the trans side, it occluded OmpF channels upon application of a cis-negative potential. The occlusion effect was negated upon changing the potential to cis positive. (C) OmpF occlusion by colicin E3 deletion constructs. 1, The presence (+) or absence (–) of occlusion denotes the ability of 0.5 to 1 µg/ml of colicin to block OmpF ion channels.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Formation of the E3/BtuB/OmpF/TolB translocon. The peripheral nature of binding of the R domain of colicin E3 to BtuB seen in the X-ray structure of the complex (25), as well as the absence of channels formed by BtuB in planar bilayers in the presence or absence of colicin (47), precludes the possibility of colicin entry through any hypothetical pore of BtuB. Thus, a secondary receptor, including OmpF and perhaps other outer membrane proteins such as the OmpC porin (32), is needed through which colicin can cross the outer membrane. Genetic evidence exists for a role of OmpF in a translocon for colicin import across the outer membrane (3). OmpF is abundant in the outer membrane (10⁵ copies/cell), and occlusion of OmpF channels incorporated in planar bilayers with colicin E3 has been well characterized (47). The existence of a colicin translocon involving the BtuB receptor, the bound colicin, and OmpF or an OmpF cluster has been postulated (21, 25, 47).

Minimum length requirement of the TolB extensor. The T and R domains of nuclease colicins E2, E3, E6, E7, and E9 are highly conserved (39), suggesting that they use similar mechanisms to import C domains with different nuclease activities. The nearly identical N-terminal 83-residue subdomain of these colicins (39) is remarkable in that it is highly flexible due to the large number of glycines (40% of residues are Gly) and contains the residues required for interaction with the Tol system. It has been proposed that flexibility of the N-terminal region enables it to cross the outer membrane and interact with the Tol system in the periplasm (11, 44). It has been found in the present study that a critical aspect for such an interaction, along with the flexibility, is the length of the peptide segment separating the Tol recognition sequence from rest of the colicin. The requirement of a minimum length of this TolB extensor in colicin E3 was determined from the properties of mutations/deletions in the 60- to 80-residue region downstream and distal to the TolB box. Binding to TolB was assessed to ensure that deletions in this region do not affect interaction with TolB. Experiments with E3 deletion constructs show that although deletions of 9 and 14 residues did not affect cytotoxicity, deletion of 19 residues in this region causes loss of cytotoxic function. When 5 residues are added back to the 19 residue deletion construct, the colicin regains its cytotoxicity, suggesting that length, rather than details of the sequence, in this region is the critical parameter. It has been shown in a previous study that a deletion construct E9 60-80 (a 21-residue deletion) did not bind to OmpF and was inactive, suggesting a second binding site for OmpF (21) in addition to an N-terminal site that includes residues 5 and/or 7 (47). However, the role of residues 60 to 80 in binding to OmpF is not clear. According to Housden et al. (21), this region is responsible for only about 15% of the colicin-OmpF interaction, with residues 1 to 7 being responsible for the remaining 85%, with deletions between residues 7 and 53 having a negligible effect on the colicin-OmpF binding. Furthermore, we have shown that deletions/mutations in the entire 60- to 80-residue region had no effect on occlusion of OmpF by colicin E3 in planar bilayer experiments. In light of the above results, it is concluded that the loss of cytotoxicity of E9 60-80 arises from the length of the deletion rather than deletion of a putative OmpF binding site. Mutation of residues 60 to 64 caused a somewhat decreased cytotoxicity, reflected in a decrease of the effective multiplicity from 14 to 7.4 (Fig. 3B and C), suggesting that these residues could participate in a weak binding to OmpF. An ordered or partially folded structure of the C-terminal segment (residues 84 to 315) of the translocation domain could explain the minimum length requirement of the subdomain found in the present study (Fig. 1A and B). An ordered T domain can result in a steric constraint on its insertion into the OmpF translocation pore and a length restriction in the TolB extensor region. The minimum length of the TolB extensor was determined to be between 19 and 23 residues.

Function of the downstream T domain: mechanism of unfolding. The limiting size of a channel through OmpF is 7 by 11 Å (12, 23), which could, in principle, allow for the threading of an unfolded polypeptide but not a folded protein. The present studies indicate that at least 60 residues of the N-terminal subdomain are able to insert through the OmpF complex to contact TolB in the periplasmic space. It is inferred that at some distance downstream of this inserted segment, there is an ordered structure in the T domain that prevents further insertion of the colicin. This leads to the following questions. (i) What is the location of this first folded domain in the T-domain? (ii) What is the function of the remaining downstream T domain, spanning residues 84 to 315, which seems not to be required for the initial insertion into the receptor? (iii) What is the mechanism of, and energy source for, unfolding of the colicin, especially of the C domain whose entry into the cell is absolutely required for cytotoxicity?

Events in colicin import across the outer membrane. It is not known whether the whole colicin or only the C domain of colicin E3 enters the cell. There is evidence that a proteolytic cleavage takes place between the R and C domains of colicin E7 during its entry into the cell (40). If such an event also occurs in colicin E3, as might reasonably be inferred, the C domain would be the only part of the colicin entering the cell.

In conclusion, the events believed to occur in colicin E3 import through the outer membrane BtuB/OmpF (and perhaps OmpC) translocon are summarized (Fig. 1C) as follows: colicin binds to BtuB; OmpF is recruited into the translocon through interaction of the T domain N terminus with OmpF; interaction of T83 with TolB in the periplasm; unfolding of the C-domain and its insertion into an OmpF channel (48); proteolytic cleavage of colicin between the C and R domains, allowing entry of the C domain into the cytoplasm, where it acts cytotoxically as an endoribonuclease.

 
These studies were supported by a grant from the NIH (GM18457) and the Henry Koffler Professorship.

We thank S. D. Zakharov and M. V. Zhalnina for helpful discussions. We also thank R. James and C. Kleanthous for providing us with the plasmid pRJ379.

 
* Corresponding author. Mailing address: Department of Biological Sciences, Purdue University, West Lafayette, IN 47907. Phone: (765) 494-4956. Fax: (765) 496-1189. E-mail: waclab{at}purdue.edu.

Published ahead of print on 3 November 2006.

Top Abstract Introduction Materials and Methods Results Discussion References

 

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Journal of Bacteriology, January 2007, p. 363-368, Vol. 189, No. 2
0021-9193/07/$08.00+0     doi:10.1128/JB.01344-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

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