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Journal of Bacteriology, January 2007, p. 455-463, Vol. 189, No. 2
0021-9193/07/$08.00+0     doi:10.1128/JB.01388-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Heme Concentration Dependence and Metalloporphyrin Inhibition of the System I and II Cytochrome c Assembly Pathways ^,

Cynthia L. Richard-Fogal, Elaine R. Frawley, Robert E. Feissner, and Robert G. Kranz^*

Department of Biology, Campus Box 1137, Washington University, 1 Brookings Drive, St. Louis, Missouri 63130¹

Received 30 August 2006/ Accepted 21 October 2006

Top Abstract Introduction Materials and Methods Results Discussion References

 
Studies have indicated that specific heme delivery to apocytochrome c is a critical feature of the cytochrome c biogenesis pathways called system I and II. To determine directly the heme requirements of each system, including whether other metal porphyrins can be incorporated into cytochromes c, we engineered Escherichia coli so that the natural system I (ccmABCDEFGH) was deleted and exogenous porphyrins were the sole source of porphyrins (hemA). The engineered E. coli strains that produced recombinant system I (from E. coli) or system II (from Helicobacter) facilitated studies of the heme concentration dependence of each system. Using this exogenous porphyrin approach, it was shown that in system I the levels of heme used are at least fivefold lower than the levels used in system II, providing an important advantage for system I. Neither system could assemble holocytochromes c with other metal porphyrins, suggesting that the attachment mechanism is specific for Fe protoporphyrin. Surprisingly, Zn and Sn protoporphyrins are potent inhibitors of the pathways, and exogenous heme competes with this inhibition. We propose that the targets are the heme binding proteins in the pathways (CcmC, CcmE, and CcmF for system I and CcsA for system II).

Top Abstract Introduction Materials and Methods Results Discussion References

 
Cytochromes c are electron transport proteins that are essential for most aerobic and anaerobic respiratory chains, as well as many other cellular processes, such as photosynthesis and apoptosis. These cytochromes are distinguished from other cytochromes by the covalent linkage of the heme vinyl groups to specific cysteine residues present in the CXXCH motif of apocytochrome c. Three different c-type cytochrome biogenesis pathways have been described, and they are called systems I, II, and III (for reviews, see references 33, 47, and 51) (systems I and II are shown in Fig. 1). System III is the simplest of the three systems and consists of a single protein, cytochrome c heme lyase, which is found in the mitochondria of certain eukaryotes (19, 40, 46). System II consists of four proteins and is found in gram-positive bacteria (35, 44), ß- and -proteobacteria (4, 30), plant chloroplasts (32, 59), and cyanobacteria (52). The most complex of the three systems, system I, consists of at least eight proteins encoded by the ccm genes and is found in - and -proteobacteria (5, 15, 41, 43), archaea (1, 26), and plant mitochondria (7, 31). Systems I and II deliver heme to the site of assembly, maintain apocytochrome c in a reduced state, and facilitate energy-independent (2, 3), covalent ligation of the heme to apocytochrome c.

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FIG. 1. Diagram of the current working model for c-type cytochrome biogenesis by systems I and II. Also shown is the ChuA heme porin pathway used in this study and the first dedicated step in heme biosynthesis. indicates targets for inhibitors used in the current study.

Progress has been made in studies on the function of many assembly proteins, but the reasons for the evolution of these three systems remain major questions. Previously, we have shown that CcmE of system I can function as a heme reservoir (24). It was also shown that the ferrochelatase inhibitor N-methyl protoporphyrin (NMPP) was able to inhibit recombinant system II at lower concentrations than it inhibited system I, leading to speculation that system I might be able to use levels of endogenous heme that are lower than the levels that system II can use. Theoretically, heme delivery (or flux) could be the limiting factor in cytochrome c biogenesis pathways under certain environmental conditions. We have recently proposed that the CcmA₂B₁C₁ release complex of system I (23) is required for using low levels of heme in the cell. This suggests that heme is bound with high affinity to CcmC and that ATP hydrolysis by the ATP binding cassette (ABC) subunit CcmA is necessary to release this molecule (as holoCcmE) to cytochrome c synthetase (CcmF/H). System II, a genetically distinct system for synthesizing holocytochromes c, does not have an ABC transporter complex or a heme chaperone, such as holoCcmE (see Fig. 1 for the model). Rather, system II has a protein called CcsA with at least six membrane-spanning helices and a periplasmic WWD domain (a putative heme binding site), like that present in CcmC or CcmF. It is possible that system II possesses a low-affinity heme binding site (in CcsA) from which direct ligation to apocytochrome c occurs (24, 28).

To directly examine the different heme affinities for the two pathways, we engineered Escherichia coli with its eight ccm genes deleted so that endogenous heme was not synthesized (by deleting hemA). Expression of chuA, encoding an outer membrane porin selective for porphyrins, allowed the ccm hemA strain (RK105) to grow like the wild type with the addition of exogenous heme. Using this approach and recombinant systems I and II, the heme dependence of each system was quantitated. As shown here with metal porphyrins, it is feasible to analyze alternative porphyrins for attachment requirements or for the discovery of pathway inhibitors.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Bacterial growth conditions. All E. coli strains used in this work were grown aerobically (with shaking at 300 rpm) at 37°C in Luria-Bertani media (Difco). Antibiotics (Sigma-Aldrich) were used at the following concentrations: carbenicillin, 100 µg ml^–1; tetracycline, 15 µg ml^–1; chloramphenicol, 20 µg ml^–1; kanamycin, 100 µg ml^–1; and ampicillin, 100 µg ml^–1. Aminolevulinic acid (ALA) (Sigma) was used at a concentration of 50 µg ml^–1 (300 µM), unless indicated otherwise. The metalloporphyrins zinc(II) protoporphyrin IX (ZnPPIX), tin(IV) protoporphyrin IX (SnPPIX), and cobalt(III) protoporphyrin IX (CoPPIX) were obtained from Frontier Scientific (Logan, UT) and were dissolved in 0.1 N NaOH to obtain 10-mg ml^–1 stock solutions. Hemin (heme; Frontier Scientific) was dissolved in 50% dimethyl sulfoxide to obtain a 10-mg ml^–1 stock solution.

Construction of E. coli strain RK105. A derivative of E. coli ccm (RK103; eight ccm genes replaced with a kanamycin cassette [24]) with the hemA gene replaced with a kanamycin resistance cassette was constructed by the procedure of Datsenko and Wanner (14). PCR products were generated with template plasmid pKD4 containing a kanamycin resistance cassette that was flanked by FLP recombinase target sites by using a pair of oligonucleotide primers, 5'-CTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTGTGTAGGCTGGAGCTGCTTC-3' and 5'-TGATGTACTGCTACTCCAGCCCGAGGCTGTCGCGCAGAATCATATGAATATCCTCCTTAG-3'. These oligonucleotide primers included sequences identical to the N-terminal and C-terminal coding sequences, respectively, of the E. coli hemA gene. The 3' ends of the oligonucleotides also included pKD4 priming sites P1 and P2, respectively. The PCR product was introduced into E. coli ccm (RK104) cured for kanamycin resistance and expressing the phage Red recombinase encoded on pKD46. Transformants were selected on media with kanamycin and ALA at 37°C. Several colonies that were resistant to kanamycin but sensitive to ampicillin (indicating a loss of pKD46) were screened by PCR with oligonucleotide primers flanking the recombination site to identify strains with the hemA gene deleted.

Construction of pRGK368. Cultures of E. coli RK103 containing pRGK334 (system II from Helicobacter pylori) (24), pRGK332 (cytochrome c₄:6xHis) (24), and pHPEX2 (55) yielded inconsistent levels of cytochrome c₄:6xHis for unknown reasons. However, the ccsBA gene cloned from Helicobacter hepaticus yielded consistent levels of cytochrome c₄:6xHis even in the presence of pHPEX2. Therefore, in the studies described here we used the H. hepaticus ccsBA-encoded system II (pRGK368) (see below), unless indicated otherwise. E. coli strain TB1 was used as the initial host for cloning. pGex-4T-1 (Amersham Biosciences)-derived vectors have an N-terminal glutathione S-transferase fusion to the insert under control of the isopropyl-ß-D-thiogalactopyranoside (IPTG)-inducible tac promoter. The H. hepaticus fused ccsBA coding region was PCR amplified from genomic DNA with a pair of oligonucleotide primers, 5'-CGCGGATCCATGATGAATATAATTAAAACACTTTTTTGTT-3' and 5'-CCGCTCGAGTTATAAATGGGGCATATCAAGCACT-3'. The amplified product was cut with BamHI and XhoI and ligated into pGEX-4T-1 to generate pRGK368 (system II plasmid). The expression of ccsBA from pRGK368 was verified by complementation of the ccm phenotype of E. coli ccm (RK103 with ccmA-H deleted) (24) containing pRGK332 (cytochrome c₄:6xHis) (data not shown).

Heme addition experiments. Cultures of E. coli RK105 (ccm hemA) harboring pRGK333 (system I; ccmA-H genes from E. coli) (24) or pRGK368 (system II), pRGK332 (cytochrome c₄:6xHis), and pHPEX2 (for expression of the ChuA outer membrane heme porphyrin receptor [55]) were grown in media overnight in the presence of 300 µM ALA. One-hundred-milliliter cultures were started from the overnight culture with 1% inocula in media devoid of ALA by incubation for 2.5 h. After 2.5 h, the 100-ml cultures had exhausted their cellular supply of ALA and required exogenous heme (or ALA) for further growth. The cultures in which the supply of ALA was exhausted were divided into 5-ml aliquots to which IPTG (1 mM; to induce synthesis of the system I proteins) and heme (0 µM to 100 µM; Frontier Scientific) were added. We discovered that heme at a concentration greater than 60 µM precipitated over time with no IPTG (1 mM) present and that heme at a concentration greater than 100 µM precipitated in the presence of IPTG. After 1 h, arabinose (0.2%; to induce synthesis of cytochrome c₄:6xHis) was added, and the cultures were grown for an additional 3 h. Cells were harvested, and protein was extracted using the B-PER protein extraction reagent (Pierce) as previously described (22). Cytochrome c₄:6xHis was purified from 200 µg of total protein over nickel affinity resin (Novagen) with two washes (10 column volumes each), one consisting of 0.5 M NaCl/20 mM Tris-HCl (pH 7.9) containing 5 mM imidazole and one consisting of 0.5 M NaCl/20 mM Tris-HCl (pH 7.9) containing 60 mM imidazole, to eliminate free heme, and then it was eluted with 100 mM EDTA. Twenty microliters of each sample was subjected to sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE), transferred to nitrocellulose, and heme stained as previously described (21). Curve fitting and heme recovery calculations were performed with the Origin scientific and analysis software package (OriginLab) using the Hill equation as the curve fit model.

NMPP inhibition experiments. Inhibition experiments were performed as previously described (24), with a few modifications. One-hundred-milliliter cultures of E. coli RK103 containing pRGK333 (system I), pRGK332 (cytochrome c₄:6xHis), and pHPEX2 (55) were inoculated (1%, vol/vol) from an overnight culture and grown with aeration at 37°C to an optical density at 600 nm (OD₆₀₀) of approximately 0.5. The mid-log-phase cultures were divided into 5-ml aliquots, to which IPTG (1 mM; to induce synthesis of the system I proteins and the chuA gene) and NMPP (0 µM to 100 µM; Frontier Scientific) were added. After 1 h, 0.2% arabinose was added (to induce synthesis of Bordetella pertussis cytochrome c₄:6xHis), and the cultures were incubated for an additional 3 h. After cells were harvested by centrifugation, soluble protein was extracted with the B-PER protein extraction reagent (Pierce) as previously described (22). Cytochrome c₄:6xHis was purified from 200 µg of total protein by using nickel affinity resin (Novagen), and 20 µl of each sample was subjected to SDS-PAGE, transferred to nitrocellulose, and heme stained as described below.

Metalloprotoporphyrin IX addition experiments. Overnight cultures of E. coli strain RK103 harboring either pRGK333 (system I) or pRGK368 (system II), pRGK332 (cytochrome c₄:6xHis), and pHPEX2 (55) were diluted into fresh media (containing the appropriate antibiotics and 8 µM hemin for the E. coli RK105 strain) and incubated with aeration at 37°C until the OD₆₀₀ was approximately 0.5. The mid-log-phase cultures were divided into 5-ml aliquots, to which IPTG (1 mM) and a metalloprotoporphyrin IX [Zn(II), Sn(IV), or Co(III) at the concentrations indicated below] were added. Arabinose (0.2%) was added after 1 h, and the cultures were incubated for an additional 3 h. Cells were harvested, and the soluble protein was extracted using B-PER (22). Cytochrome c₄:6xHis was purified from 300 µg of total protein, and 20 µl was subjected to SDS-PAGE and transferred to nitrocellulose. Prior to heme staining and cytochrome c₄ immunoblotting, the nitrocellulose blots were screened for fluorescence with an LAS-1000 plus luminescent image analyzer charge-coupled device camera system (Fujifilm, Tokyo, Japan).

NMPP-ZnPPIX combined inhibition experiments. Overnight cultures of the E. coli RK103 strain harboring pRGK333 (system I), pRGK332 (cytochrome c₄:6xHis), and pHPEX2 were diluted into 100 ml of fresh media containing the appropriate antibiotics and incubated at 37°C with aeration until the OD₆₀₀ was approximately 0.5. The cultures were divided into 5-ml aliquots, IPTG (1 mM) and NMPP (60 µM and 100 µM) were added to separate cultures, and the cultures were incubated for 30 min. ZnPPIX (5 µM) was then added to each culture, and the cultures were incubated for an additional 30 min. Arabinose (0.2%) was added, and the cultures were incubated for an additional 3 h. Cells were harvested by centrifugation, and the soluble protein was extracted with B-PER, as previously described (22). The soluble protein was processed as described above for the metalloprotoporphyrin addition experiments.

Other methods. Protein concentrations were determined with a bicinchoninic acid assay kit (Pierce), using bovine serum albumin as a standard. Heme staining and Western blotting were performed as previously described (21), using the SuperSignal Femto chemiluminescent substrate (Pierce). For heme stain quantitation we used LOLITA II (low light test array; raytest USA, Wilmington, NC) for standardization of detection of light intensity with an LAS-1000 plus luminescent image analyzer charge-coupled device camera system. The reduced (10 mM sodium dithionite) and oxidized (10 mM ammonium persulfate) absorption spectra were obtained with a Shimadzu UV-2101PC scanning spectrophotometer using nickel affinity-purified holocytochrome c₄:6xHis.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Exogenous porphyrin approach: characterization of E. coli ccm hemA with a porphyrin porin. Previously, we deleted the ccmA-H genes in E. coli using a kanamycin resistance cassette (RK103) (24). To construct a heme-dependent strain, we initially screened for excision of this kanamycin resistance cassette. With the ccm kanamycin-sensitive strain (RK104), the hemA gene was replaced with a kanamycin resistance cassette. The hemA gene codes for glutamyl-tRNA reductase, the first committed enzyme in heme biosynthesis, and E. coli cells lacking hemA require exogenous ALA for growth (58) (Fig. 1). The ccm hemA strain (RK105) requires ALA in the growth medium to form colonies (not shown). RK105 with recombinant system I (pRGK333) or system II (pRGK334) also requires exogenous ALA for growth (see Fig. S1 in the supplemental material). To determine if exogenous ALA facilitates synthesis of cytochromes c, we transformed these strains with pRGK332, which expresses a C-terminally hexahistidine-tagged B. pertussis cytochrome c₄ under control of an arabinose-inducible promoter (24). E. coli RK105 with pRGK333 or pRGK334 and pRGK332 was unable to synthesize holocytochrome c₄:6xHis in the presence of 60 µM ALA (Fig. 2, lanes 1, 2, 6, and 7) but was able to synthesize holocytochrome c₄:6xHis in the presence of higher ALA concentrations (Fig. 2, lanes 3, 4, 5, 8, 9, and 10). As noted previously (24), in addition to the diheme 24-kDa cytochrome c₄, a proteolyzed monoheme 12-kDa cytochrome c₄:6xHis* was produced. We concluded that exogenous ALA is required for growth and holocytochrome c production in RK105.

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FIG. 2. Heme staining of cytochrome c4 with increasing concentrations of ALA. E. coli RK105 cultures containing pRGK333 (lanes 1 to 5) or pRGK334 (lanes 6 to 10) were diluted into fresh media without ALA, depleting the intracellular ALA. ALA was added (concentrations are indicated above the lanes) along with 1 mM IPTG for 1 h. Cytochrome c4:6xHis was induced with 0.2% arabinose for 3 h, and soluble B-PER protein extracts were prepared. System I indicates pRGK333, System II indicates pRGK334, and c4:6xHis indicates pRGK332. cyt c4, cytochrome c4; cyt c4*, cytochrome c4*.

To determine if exogenous heme rather than ALA could support growth, a culture of RK105 in which ALA was exhausted was supplemented with different concentrations of heme (up to 100 µM). Growth was not detected until 4 or 5 days after the original inoculation, even at high heme concentrations. No holocytochrome c₄:6xHis was detected in cultures that contained either the system I or system II plasmid and the cytochrome c₄:6xHis reporter under these conditions.

Previous reports have indicated that the outer membranes of some strains of E. coli are poorly permeable to heme (8, 38, 57), and for this reason we expressed the heme porin (chuA) from pHPEX2 (55). The gene was obtained from E. coli O157:H7 and was expressed from the IPTG-inducible lacUV5 promoter. ChuA is a member of a class of relatively nonspecific enterobacterial heme receptors that are TonB dependent and facilitate heme acquisition (Fig. 1) (references 49 and 53 and references therein). ChuA, by analogy with the Yersinia enterocolitica HemR outer membrane heme receptor, recognizes free heme and heme bound to a variety of proteins (49). Growth of E. coli RK105 containing either pRGK333 or pRGK334 and pHPEX2 was dependent on exogenous heme (Fig. 3). Expression of chuA (pHPEX2) allowed growth at nearly wild-type levels, suggesting that there was efficient uptake of exogenous heme, whereas when the ALA in the culture was depleted and heme was not added, no growth was detectable (Fig. 3). pHPEX2 also facilitated the heme-dependent production of B. pertussis cytochrome c₄:6xHis when the plasmids with system I or system II were present (see below).

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FIG. 3. Growth of E. coli RK105 with exogenous heme. Overnight cultures of E. coli RK105 (ccm hemA) or cultures containing pHPEX2 and pRGK333 (A) or pRGK334 (B) were diluted into media without ALA or heme. The cultures were incubated aerobically at 37°C for 2.5 h to exhaust the ALA. Heme was added at the concentrations indicated, and growth (A600) was measured. For reference, the growth of E. coli RK103 (wild type) containing either pRGK333 or pRGK334 is shown (). SysI, system I; SysII, system II.

System I acquires heme at significantly lower concentrations than system II acquires heme. To directly examine the differences in heme acquisition between systems I and II, we added various concentrations of exogenous heme to E. coli RK105 containing pHPEX2, pRGK332, and one of the system plasmids. (As noted in Materials and Methods, in these studies we used a system II ccsBA fusion from H. hepaticus, encoded by pRGK368, which is compatible with pHPEX2, which resulted in consistent levels of the cytochrome c₄:6xHis reporter.) Overnight starter cultures (grown in the presence of ALA) were used to inoculate media devoid of ALA and incubated for 2.5 h to exhaust the cellular ALA (and heme); IPTG, arabinose, and heme were then added. The cytochrome c₄ acted as a trap, allowing quantitation of the levels of heme that fluxed through each system. Cytochrome c₄:6xHis was purified over nickel chelating resin to eliminate free heme, and both the 24-kDa and 12-kDa forms were quantitated by heme staining (Fig. 4A and C). For system I, since maximum synthesis of holocytochrome c₄:6xHis occurs with less than 10 µM heme, in all subsequent experiments using system I we used heme levels between 0 µM and 10 µM. Averaged over four trials for system I, the holocytochrome c₄:6xHis levels were restored to 50% of the maximum levels achieved with exogenous heme when we used 1.9 ± 0.4 µM heme (Fig. 4B). For experiments with system II (CcsBA), increases in the holocytochrome c₄:6xHis signal were also observed as the heme concentration increased, and heme concentrations of 30 µM to 40 µM resulted in maximum synthesis (Fig. 4C). Averaged over five trials, the holocytochrome c₄:6xHis levels reached 50% of the maximum levels when we used 18.6 µM ± 8.1 µM heme for system II (Fig. 4D). We concluded that the apparent affinity for heme of system I is at least fivefold higher than the apparent affinity for heme of system II.

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FIG. 4. Exogenous heme acquisition profiles for system I and system II. An E. coli heme auxotroph (RK105) containing pRGK333 or pRGK368, pRGK332, and pHPEX2 (outer membrane heme porin) was grown and treated as described in Materials and Methods. (A and C) Heme staining of holocytochrome c4 from representative trials with system I (A) and system II (C). (B and D) Curve fits to average heme staining intensity expressed as a percentage of the maximum signal intensity with respect to the heme concentrations for system I (n = 4) (B) and system II (n = 5) (D). cytc4, cytochrome c4.

Zinc protoporphyrin IX is not incorporated into the B. pertussis cytochrome c₄:6xHis biogenesis reporter. It has not been determined whether any of the three cytochrome c biogenesis systems can incorporate other metal porphyrins into the CXXCH motif of a c-type cytochrome. If they can, then iron itself is not critical for the attachment mechanism. The porphyrin porins like ChuA also facilitate permeability of other metal porphyrins (48). Using the approach described here, we tested whether heme can be replaced by other metalloporphyrins in the c-type cytochrome (cytochrome c₄:6xHis) reporter. We chose three metalloporphyrins to screen for incorporation into cytochrome c₄:6xHis: ZnPPIX, SnPPIX, and CoPPIX. These three metal protoporphyrins were selected because all three metals have been successfully inserted in vitro into horse heart cytochrome c by directly replacing the natural iron with the metal (17, 54).

To determine if ZnPPIX, SnPPIX, or CoPPIX could be inserted in place of heme into cytochrome c₄:6xHis, we grew cultures of E. coli RK105 containing pRGK333 (system I), pRGK332 (cytochrome c₄:6xHis), and pHPEX2 with heme to an OD₆₀₀ of 0.5. Then IPTG and metalloporphyrin (8 µM, 12.5 µM, or 25 µM) were added, and the cultures were incubated for 1 h. Arabinose was added to a concentration of 0.2% to induce the synthesis of cytochrome c₄:6xHis, and the cultures were incubated for three more hours before cells were harvested. As expected, heme was detected on cytochrome c₄:6xHis when either 8 µM or 25 µM heme alone was added (Fig. 5, lanes 1 and 2, respectively). When ZnPPIX was present along with 8 µM heme, decreasing amounts of holocytochrome c₄ were detected as the ZnPPIX concentration increased (Fig. 5, lanes 3 to 5). With 25 µM ZnPPIX (Fig. 5, lane 5) the level of heme on cytochrome c₄:6xHis was barely detectable. Concentrations of ZnPPIX greater than 25 µM did not further reduce the level of detectable heme (not shown). SnPPIX also reduced the level of detectable heme on cytochrome c₄:6xHis (see below), but CoPPIX (at concentrations up to 100 µM) had no affect. It is possible that ZnPPIX (or SnPPIX), when present with 8 µM heme, competes with heme for ChuA-dependent transport into the cell. We therefore tested an E. coli ccm strain (RK103) in which there is endogenous heme production to determine if ZnPPIX (or SnPPIX) reduced the holocytochrome c₄ levels detectable by heme staining. When cultures of E. coli RK103 with pRGK333 (system I), pRGK332, and pHPEX2 were treated with ZnPPIX, the level of holocytochrome c₄:6xHis decreased as the concentration of ZnPPIX was increased (Fig. 6A, lanes 1 to 5). For E. coli RK103 with pRGK368 (system II), pRGK332, and pHPEX2 there were also decreases in the level of holocytochrome c₄:6xHis with even lower concentrations of ZnPPIX (Fig. 6B, lanes 1 to 10) (note that the levels of ZnPPIX were nanomolar).

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FIG. 5. Heme staining of holocytochrome c4:6xHis with increasing concentrations of ZnPPIX. E. coli RK105 cultures containing pRGK333, pRGK332, and pHPEX2 were grown in the presence of 8 µM heme (lanes 1 and 3 to 5) or 25 µM heme (lane 2) to an OD600 of approximately 0.5. ZnPPIX was added (lanes 3 to 5), and 1 mM IPTG was added to induce synthesis of the system I proteins. The cultures were incubated for 1 h, and 0.2% arabinose was added for 3 h to induce the synthesis of cytochrome c4. The concentrations (in µM) of heme and ZnPPIX are indicated above the lanes. SysI, pRGK333; c4:6xHis, pRGK332; cyt c4, cytochrome c4.

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FIG. 6. Heme staining for detection of holocytochrome c4 synthesis in the presence of ZnPPIX. E. coli RK103 cultures containing either pRGK333, pRGK332, and pHPEX2 (A) or pRGK368, pRGK332, and pHPEX2 (B) were grown and treated as described in Materials and Methods. The concentrations of ZnPPIX are indicated above the lanes. SysI, pRGK333; SysII, pRGK368; c4:6xHis, pRGK332; ccm, E. coli RK103; cyt c4, cytochrome c4; cyt c4*, cytochrome c4*.

For SnPPIX and CoPPIX, decreases in the level of holocytochrome c₄:6xHis were observed as the concentration of SnPPIX was increased (see Fig. S2A, lanes 1 to 5, and Fig. 2B, lanes 1 to 10, in the supplemental material). Addition of CoPPIX (at concentrations up to 100 µM) resulted in no decrease, on average, in the level of heme on cytochrome c₄:6xHis, suggesting that CoPPIX is not incorporated and is not an inhibitor (see below).

Based on the results described above, we decided to focus on the effects of ZnPPIX, which at low concentrations significantly reduced the holo(heme)cytochrome c levels detected by heme staining. ZnPPIX does not react with the chemiluminescent substrate used to stain heme on holocytochrome c₄ (unpublished). The absence of heme staining could indicate that ZnPPIX either (i) competes with heme and is incorporated into cytochrome c₄:6xHis or (ii) specifically inhibits c-type cytochrome biogenesis (i.e., attachment of heme).

Cytochrome c₄ covalently bound to ZnPPIX would be highly fluorescent, a property of ZnPPIX proteins (20). We were unable to detect fluorescence of ZnPPIX in purified cytochrome c₄:6xHis preparations in these experiments, suggesting that ZnPPIX is not incorporated. To confirm that ZnPPIX is not incorporated into cytochrome c₄, we obtained visible absorption spectra of nickel-purified cytochrome c₄:6xHis isolated from E. coli RK103 harboring pRGK333 (system I), pRGK332 (cytochrome c₄:6xHis), and pHPEX2 grown with 8 µM ZnPPIX (Fig. 7A). Note that at this concentration approximately 20% of the wild-type level of holocytochrome was produced (Fig. 6A, lane 3). The sodium hydrosulfite-reduced peak at 552 nm is characteristic of cytochrome c₄ with a c-type linkage to heme (9, 10). If ZnPPIX had been incorporated, we would have expected ß and peaks at 549 and 585 nm, respectively (54). In addition, electrospray ionization-mass spectrometry (ESI-MS) analysis of the same protein preparation showed that holocytochrome c₄* (12-kDa proteolytic fragment) contained only heme, as ZnPPIX incorporation would have yielded a protein that was 10 mass units larger (Fig. 7B and inset). These results confirm that ZnPPIX is not incorporated into cytochrome c₄:6xHis but instead inhibits biogenesis.

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FIG. 7. Reduced and oxidized absorption spectra and ESI-MS analysis of cytochrome c4 produced in the presence of ZnPPIX. Overnight cultures of E. coli RK103 containing pRGK333 (system I), pRGK332 (cytochrome c4:6xHis), and pHPEX2 were diluted into fresh media and grown to the mid-log phase. ZnPPIX (8 µM) and IPTG (1 mM) were added, and the cultures were incubated for an additional 1 h. Arabinose (0.2%) was added for 3 h to induce synthesis of cytochrome c4:6xHis, and soluble B-PER extracts were prepared. (A) Reduced (sodium hydrosulfite) and oxidized (ammonium persulfate) absorption spectra. (B) ESI-MS analysis.

We also performed cytochrome c₄ immunoblotting for nickel affinity-purified cytochrome c₄:6xHis from cultures grown as described above in the presence of SnPPIX. It has been documented that apocytochromes c in mutants in which heme is unable to attach are susceptible to natural proteases, presumably because they do not fold properly (4, 18, 29). This has been shown with the B. pertussis cytochrome c₄:6xHis reporter used in these studies (4, 24). Therefore, if SnPPIX is incorporated into apocytochrome c, we would expect that the holocytochrome would be stable and not subject to natural proteolysis. When antiserum to B. pertussis cytochrome c₄ was used (see Fig. S2 in the supplemental material), decreases in the levels of cytochrome c₄:6xHis were detected for both system I (see Fig. S2A, lanes 6 to 10, in the supplemental material) and system II (see Fig. S2B, lanes 11 to 20, in the supplemental material).

ZnPPIX and SnPPIX are specific inhibitors of system I and system II. Our results suggest that ZnPPIX and SnPPIX are not incorporated into cytochrome c₄:6xHis by either system but instead inhibit biogenesis. The inhibition of holocytochrome c₄:6xHis synthesis with system I required significantly higher concentrations of ZnPPIX and SnPPIX than the inhibition of holocytochrome c₄:6xHis synthesis with system II required in these experiments (Fig. 6; see Fig. S2 in the supplemental material). The concentration of SnPPIX required for inhibition of holocytochrome c₄:6xHis synthesis was also higher than the concentration of ZnPPIX required for inhibition of holocytochrome c₄:6xHis synthesis for both systems. The concentration of ZnPPIX required for 50% inhibition of system I was approximately 2 µM and the concentration of ZnPPIX required for 50% inhibition of system II was approximately 25 nM, whereas the concentration of SnPPIX required for 50% inhibition of system I was approximately 65 µM and the concentration of SnPPIX required for 50% inhibition of system II was approximately 18 µM.

To determine if ZnPPIX affects some basic cellular processes, growth studies were performed with E. coli RK103 harboring pRGK333 (system I), pRGK332 (cytochrome c₄:6xHis), and pHPEX2 in the presence and absence of ZnPPIX. In cultures with either 12.5 µM or 25 µM ZnPPIX there was no decrease in the growth rate or yield compared to the growth rate or yield for the same strain without ZnPPIX (see Fig. S3 in the supplemental material). ZnPPIX has previously been shown to inhibit the final enzyme in heme biosynthesis, ferrochelatase, from mice at a K_i of 4.5 µM (13); however, because no growth defect was observed with 12.5 µM or 25 µM ZnPPIX, we concluded that ferrochelatase was not inhibited in our system with these concentrations of ZnPPIX. In addition, using a plasmid with cytochrome c₄:Pho (B. pertussis cytochrome c₄ alkaline phosphatase fusion [24]) that was induced with arabinose, alkaline phosphatase was detectable at equivalent levels in the presence of ZnPPIX (see Fig. S4A, lanes 6 and 7, in the supplemental material). These results indicate that ZnPPIX does not inhibit transcription, translation, or secretion of cytochrome c₄, further suggesting that ZnPPIX specifically inhibits some step(s) in c-type cytochrome biogenesis.

ZnPPIX specifically inhibits system I c-type cytochrome biogenesis after holoCcmE synthesis. To begin to examine where ZnPPIX inhibits system I c-type cytochrome biogenesis, we assayed for the presence of heme on cytochrome c₄:6xHis when both NMPP and ZnPPIX were included in the culture. NMPP is a strong inhibitor of ferrochelatase and was used to control cellular heme levels (12, 25, 39). With NMPP inhibition in E. coli (RK103) containing pRGK333 (system I) and pRGK332 (cytochrome c₄:6xHis), holocytochrome c synthesis was reduced to a basal level of approximately 38% (24). This result was shown to be due to the ability of the CcmE protein to act as a reservoir for heme, permitting residual (38%) synthesis of holocytochrome c₄:6xHis when 100 µM NMPP was added to the culture (24). Thus, in these experiments holoCcmE was present at levels that allowed 38% holocytochrome c₄ production when ferrochelatase was inhibited. If ZnPPIX inhibits synthesis significantly more than NMPP inhibits synthesis, it must act after the formation of this heme reservoir since some holoCcmE was present at the time of ZnPPIX addition (Fig. 1). Cultures of E. coli (RK103) containing pRGK333 (system I), pRGK332 (cytochrome c₄:6xHis), and pHPEX2 were grown and treated as described in Materials and Methods (NMPP-ZnPPIX combined inhibition experiments). In addition, individual inhibition experiments were performed with cultures that contained NMPP or ZnPPIX (Fig. 8). When both NMPP and ZnPPIX (Fig. 8) were present, the holocytochrome c₄:6xHis levels dropped from approximately 54% with 60 µM NMPP alone (Fig. 8) to approximately 7% with 60 µM NMPP and 5 µM ZnPPIX and from approximately 30% with 100 µM NMPP (Fig. 8) to 0% with 100 µM NMPP and 5 µM ZnPPIX. These results are consistent with previous data showing that ZnPPIX inhibits holocytochrome c₄:6xHis production so that the levels are nearly undetectable (Fig. 6A, lane 5) and inhibits this production considerably more than NMPP inhibits it.

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FIG. 8. Holocytochrome c4 synthesis in the presence of ZnPPIX and NMPP. E. coli RK103 cultures containing pRGK333 (system I), pRGK332 (cytochrome c4:6xHis), and pHPEX2 were grown and treated as described in Materials and Methods. Independent (NMPP and ZnPPIX) inhibition experiments were also performed. Holocytochrome c4 was quantified on the basis of heme staining intensity (in arbitrary units) in three independent trials.

Our results suggest that ZnPPIX inhibits downstream of holoCcmE since this "heme reservoir" is no longer available for cytochrome c₄ biogenesis. Thus, it is feasible that the WWD-containing protein CcmF is the target for ZnPPIX (see Discussion) (Fig. 1). If the target for ZnPPIX is a heme binding protein (site), then exogenous heme should compete with this inhibition (i.e., ZnPPIX binding). To test this hypothesis, we used the arabinose-inducible cytochrome c₄:Pho fusion, whose holocytochrome c₄ component can be assayed by heme staining in E. coli RK103 harboring system I and pHPEX2. In cultures grown with ZnPPIX and increasing concentrations of exogenous heme there were corresponding increases in holocytochrome c₄:Pho (see Fig. S4B, lanes 1 to 5, in the supplemental material). The same heme-stained polypeptides were confirmed to be full-length cytochrome c₄:Pho fusion proteins by Western blotting (see Fig. S4A, lanes 1 to 5, in the supplemental material). We also demonstrated this heme competition with ZnPPIX using the cytochrome c₄:6xHis reporter. In the presence of 8 µM ZnPPIX (system I) (see Fig. S4C, left panel, in the supplemental material) or 2 µM ZnPPIX (system II) (see Fig. S4C, right panel, in the supplemental material), increasing concentrations of exogenous heme resulted in corresponding increases in holocytochrome c₄:6xHis levels. We concluded that heme is able to compete with ZnPPIX inhibition and that ZnPPIX is a specific inhibitor of c-type cytochrome biogenesis by either system I or system II.

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Heme affinities of system I compared to those of system II. We have shown previously that when heme biosynthesis is inhibited by the ferrochelatase inhibitor NMPP, cytochrome c assembly persists at higher inhibitor concentrations with system I than with system II (24). We concluded that lower endogenous levels of heme can be used by the system I pathway. However, with this method we were not able to directly measure heme levels, and the use of inhibitors clearly has caveats. For example, the possibility that NMPP interferes with cytochrome c biogenesis directly could not be ruled out. Here we developed an approach to directly control heme levels and quantify the product of biogenesis, cytochrome c. We found that the level of exogenous heme utilization by system I is at least fivefold lower than the level of exogenous heme utilization by system II. The heme concentration dependence curves (Fig. 4) also reveal differences in kinetics between system I and system II. System I synthesis exhibits a sigmoidal curve and is saturated at low heme levels (around 3 µM), whereas system II synthesis shows a linear response with a reduced slope, more reminiscent of a diffusional process. We argue that these heme dependence results represent a true reflection of the relative differences between systems I and II. Such comparisons must be performed with the same genetic background, as was done here. With two different organisms, each with a natural system I or system II, it would be impossible to ensure or even expect that exogenous heme levels reflect the same internal levels (for any given concentration).

With respect to the study with E. coli described here, it is likely that the recombinant, functional system II operates in an environment similar to its natural environment. Other proteobacteria (e.g., B. pertussis and Helicobacter) probably obtained their natural system II genes by lateral transfer (30), indicating that the gram-negative periplasm and inner membrane are appropriate environments. In fact, recently it has been discovered that Bordetella parapertussis has the genes for both system I and system II (47), making it even more likely that the gram-negative envelope provides an environment suitable for the use of either system. Clearly, selective pressures have maintained one system or the other, but only in this rare case have they maintained both. Here we propose that heme availability in natural habitats is an important pressure.

Because heme biosynthesis depends on central metabolism (e.g., glutamate or succinyl coenzyme A and glycine), a limiting factor for heme biosynthesis is often the free iron levels (27, 42, 56). Based on the presence of the "heme reservoir" CcmE (24) and the fivefold-higher affinity for heme, we speculate that organisms in which heme synthesis is limited, such as organisms faced with low-iron environments, would have an advantage using system I. Concerning advantages for system II, the synthesis of fewer accessory components (and not requiring ATP hydrolysis for heme flux through the system) may confer benefits to some organisms, particularly when they do not need to scavenge for heme or iron.

Metal porphyrins as inhibitors but not prosthetic groups for cytochrome c assembly. While replacement of the central Fe in horse heart cytochrome c with Zn (54), Co (16), and Sn (54) by in vitro manipulation has been demonstrated, the use of alternative metal porphyrins by cytochrome c biogenesis systems has not been tested. Here various methods to detect ZnPPIX (and SnPPIX) insertion into apocytochrome c by either the system I or II cytochrome c biogenesis pathway were employed. No evidence for insertion was obtained, suggesting that there is specificity for iron protoporphyrin IX (FePPIX), at least for system I and system II. This inability may be related to the specific system I and system II attachment mechanism(s). Because little is known about the synthetase mechanism, a future goal is to understand the specific need for FePPIX rather than other metal protoporphyrins.

Surprisingly, ZnPPIX and SnPPIX inhibit cytochrome c biogenesis. Heme competes with these inhibitors (see Fig. S4B and C in the supplemental material), suggesting that proteins in the pathway that bind heme are the likely targets. It has also been suggested that heme binding proteins are targets of noniron metal porphyrins by Stojiljkovic and colleagues, who found that certain noniron metal porphyrins have antibacterial properties (48). It is important to note that the targets that these authors proposed (e.g., oxidases) are not the same targets that we describe here. For example, Stojiljkovic et al. showed that gallium protoporhyrin IX inhibits E. coli growth only under aerobic iron-limiting conditions. However, wild-type E. coli normally does not synthesize c-type cytochromes under any aerobic conditions. We observed no inhibition of E. coli growth with ZnPPIX under aerobic conditions in liquid media (even with a porphyrin porin). This suggests that the heme biosynthetic enzyme ferrochelatase was not the target in our studies. Ferrochelatase from mice is inhibited by ZnPPIX with a K_i of 4.5 µM (13), but apparently the bovine enzyme is not significantly inhibited (11). The E. coli enzyme has not been tested to our knowledge. Stojiljkovic and colleagues (48) showed that there was growth inhibition of Staphylococcus aureus by gallium protoporhyrin IX and ZnPPIX but not by SnPPIX. S. aureus does not have c-type cytochromes (or system I or II [34]), and thus their putative target(s) are clearly different than the targets studied here. It is feasible that the inhibition observed by Stojiljkovic et al. (48) was due to the previously demonstrated inhibition of ferrochelatase activity (13).

The system I pathway has three possible sites of interaction with ZnPPIX: the proposed heme interacting proteins CcmC and CcmF (via the WWD domain) and the "heme reservoir" on the chaperone protein CcmE. Here we show that ZnPPIX further reduces the incomplete NMPP inhibition of system I holocytochrome c biosynthesis to zero, thus inhibiting downstream of the CcmE reservoir (e.g., CcmF). (These experiments did not rule out the possibility that there is an additional interaction of ZnPPIX with the CcmC and/or the CcmE proteins.) In the case of system II, we postulate that heme diffuses to the periplasmic WWD domain of CcsA for ligation to apocytochrome c. Thus, there is only one likely target for ZnPPIX/SnPPIX inhibition in the system II pathway, which is the WWD domain of CcsA.

ZnPPIX and SnPPIX are potent inhibitors of another heme binding enzyme, heme oxygenase (HO-1) (37), which is responsible for the oxidative cleavage of heme that yields free iron, carbon monoxide, and biliverdin (50). Metalloporphyrins compete with FePPIX for binding to the heme-binding pocket of HO-1, but due to the closed shell nature of zinc and tin, these metalloporphyrins cannot undergo oxidation-reduction reactions, and thus they act as inhibitors of HO-1 rather than substrates (reference 36 and references therein). CoPPIX, recognized mainly for its ability to induce HO-1 synthesis by an unknown mechanism (45), is also a weak inhibitor of HO-1 enzymatic activity (37). Metalloporphyrins have also been found to irreversibly inhibit both caspase-3 and caspase-8, neither of which is a heme binding protein, by binding to the active site and blocking the binding of a specific substrate (for example, caspase-3 with poly-ADP ribose polymerase) (6). This inhibition was found to be mainly dependent on the porphyrin ring structure as metal-free protopophyrin IX also exhibited inhibition, but a role for the central metal ion could not be ruled out (6). Inhibition of cytochrome c assembly pathways I and II adds two more specific targets for metal porphyrins. The approach used here should facilitate further analysis of cytochrome c synthetase mechanisms and discovery of other inhibitors of these systems.

 
We thank Douglas Goodwin, Auburn University, for providing plasmid pHPEX2 and James Fox, Massachusetts Institute of Technology, for providing H. hepaticus.

This work was supported by NIH grant GM47909 to R.G.K.

 
* Corresponding author. Mailing address: Department of Biology, Campus Box 1137, Washington University, 1 Brookings Drive, St. Louis, MO 63130. Phone: (314) 935-4278. Fax: (314) 935-4432. E-mail: kranz{at}biology.wustl.edu.

Published ahead of print on 3 November 2006.

Supplemental material for this article may be found at http://jb.asm.org/.

Present address: Department of Pharmacology and Physiology, University of Rochester, Rochester, NY 14642.

Top Abstract Introduction Materials and Methods Results Discussion References

 

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Journal of Bacteriology, January 2007, p. 455-463, Vol. 189, No. 2
0021-9193/07/$08.00+0     doi:10.1128/JB.01388-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

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