Files in this Data Supplement:

• Supplemental file 1 - Supplemental methods; legends to Fig. S1 to S5
  MS Word document, 30K.
• Supplemental file 2 - Table S1, strains and sequence accession numbers used for MLSA; Table S2, primers for the MLSA for Vibrionaceae; Table S3, pairwise amino acid sequence divergence and estimated radiation times among clades; Fig. S1, chromosomal locations of gene fragments; Fig. S2, unrooted phylogenetic tree of 58 members of the Vibrionaceae; Fig. S3, clades with species not included in the analysis based on nine loci; Fig. S4, splits network; Fig. S5, interspecies resolution of nine loci available for MLSA of Vibrionaceae
  Zipped PowerPoint document, 187K.